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P09450 (JUNB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor jun-B
Alternative name(s):
MyD21
Gene names
Name:Junb
Synonyms:Jun-b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Subunit structure

Binds DNA as a homodimer or as a heterodimer with another member of the Jun/Fos family By similarity. Interacts with NFE2 (via its WW domains) By similarity.

Subcellular location

Nucleus.

Induction

By growth factors.

Post-translational modification

Ubiquitinated by ITCH, leading to its degradation By similarity.

Sequence similarities

Belongs to the bZIP family. Jun subfamily.

Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   PTMAcetylation
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular process

Inferred from direct assay PubMed 11818961. Source: MGI

cellular response to calcium ion

Inferred from direct assay PubMed 19303854. Source: MGI

cellular response to hormone stimulus

Inferred from electronic annotation. Source: Ensembl

decidualization

Inferred from mutant phenotype PubMed 10022836. Source: MGI

embryonic process involved in female pregnancy

Inferred from mutant phenotype PubMed 10022836. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 10022836. Source: MGI

labyrinthine layer blood vessel development

Inferred from mutant phenotype PubMed 10022836. Source: MGI

osteoblast differentiation

Inferred from mutant phenotype PubMed 14769860. Source: MGI

osteoblast proliferation

Inferred from mutant phenotype PubMed 14769860. Source: MGI

osteoclast differentiation

Inferred from mutant phenotype PubMed 14769860. Source: MGI

positive regulation of cell differentiation

Inferred from direct assay PubMed 11456449. Source: MGI

regulation of cell cycle

Inferred from direct assay PubMed 11818961. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 11818961. Source: MGI

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to corticosterone

Inferred from electronic annotation. Source: Ensembl

response to cytokine

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to light stimulus

Inferred from electronic annotation. Source: Ensembl

response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

response to peptide hormone

Inferred from electronic annotation. Source: Ensembl

response to progesterone

Inferred from electronic annotation. Source: Ensembl

transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

trophectodermal cell differentiation

Inferred from direct assay PubMed 11456449. Source: MGI

vasculogenesis

Inferred from mutant phenotype PubMed 10022836. Source: MGI

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12220541. Source: MGI

RNA polymerase II regulatory region sequence-specific DNA binding

Inferred from direct assay PubMed 19289495. Source: MGI

double-stranded DNA binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 19578362PubMed 21572431PubMed 22983707PubMed 22992523PubMed 22992524. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Transcription factor jun-B
PRO_0000076439

Regions

Domain265 – 32864bZIP
Region265 – 29228Basic motif By similarity
Region293 – 32129Leucine-zipper By similarity

Amino acid modifications

Modified residue1021Phosphothreonine By similarity
Modified residue1041Phosphothreonine By similarity
Modified residue1171Phosphoserine By similarity
Modified residue2371N6-acetyllysine Ref.7
Modified residue2481Phosphoserine By similarity
Modified residue2521Phosphothreonine By similarity
Modified residue2561Phosphoserine Ref.6

Experimental info

Sequence conflict531P → S in BAC40473. Ref.3
Sequence conflict2001G → R in AAA74916. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09450 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 6F52D4FECC32E234

FASTA34435,765
        10         20         30         40         50         60 
MCTKMEQPFY HDDSYAAAGY GRSPGSLSLH DYKLLKPTLA LNLADPYRGL KGPGARGPGP 

        70         80         90        100        110        120 
EGSGAGSYFS GQGSDTGASL KLASTELERL IVPNSNGVIT TTPTPPGQYF YPRGGGSGGG 

       130        140        150        160        170        180 
TGGGVTEEQE GFADGFVKAL DDLHKMNHVT PPNVSLGASG GPQAGPGGVY AGPEPPPVYT 

       190        200        210        220        230        240 
NLSSYSPASA PSGGSGTAVG TGSSYPTATI SYLPHAPPFA GGHPAQLGLS RGASAFKEEP 

       250        260        270        280        290        300 
QTVPEARSRD ATPPVSPINM EDQERIKVER KRLRNRLAAT KCRKRKLERI ARLEDKVKTL 

       310        320        330        340 
KAENAGLSSA AGLLREQVAQ LKQKVMTHVS NGCQLLLGVK GHAF 

« Hide

References

« Hide 'large scale' references
[1]"A gene activated by growth factors is related to the oncogene v-jun."
Ryder K., Lau L.F., Nathans D.
Proc. Natl. Acad. Sci. U.S.A. 85:1487-1491(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complex genetic organization of junB: multiple blocks of flanking evolutionarily conserved sequence at the murine and human junB loci."
Phinney D.G., Tseng S.W., Ryder K.
Genomics 28:228-234(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
Tissue: Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[5]"Complexity of the immediate early response of myeloid cells to terminal differentiation and growth arrest includes ICAM-1, Jun-B and histone variants."
Lord K.A., Hoffman-Liebermann B., Liebermann D.A.
Oncogene 5:387-396(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-30.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-256, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-237, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03236 mRNA. Translation: AAA39343.1.
U20735 Genomic DNA. Translation: AAA74916.1.
AK088643 mRNA. Translation: BAC40473.1.
BC003790 mRNA. Translation: AAH03790.1.
X54332 mRNA. No translation available.
CCDSCCDS22488.1.
PIRTVMSJB. A28963.
RefSeqNP_032442.1. NM_008416.3.
UniGeneMm.1167.

3D structure databases

ProteinModelPortalP09450.
SMRP09450. Positions 269-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200872. 4 interactions.
DIPDIP-1069N.
IntActP09450. 1 interaction.

PTM databases

PhosphoSiteP09450.

Proteomic databases

PRIDEP09450.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000064922; ENSMUSP00000064680; ENSMUSG00000052837.
GeneID16477.
KEGGmmu:16477.
UCSCuc009mop.1. mouse.

Organism-specific databases

CTD3726.
MGIMGI:96647. Junb.

Phylogenomic databases

eggNOGNOG283376.
HOGENOMHOG000006648.
HOVERGENHBG001722.
InParanoidP09450.
KOK09028.
OMALNAYCPN.
OrthoDBEOG75MVXV.
PhylomeDBP09450.

Gene expression databases

ArrayExpressP09450.
BgeeP09450.
CleanExMM_JUNB.
GenevestigatorP09450.

Family and domain databases

Gene3D1.10.880.10. 1 hit.
InterProIPR004827. bZIP.
IPR005643. JNK.
IPR002112. Leuzip_Jun.
IPR008917. TF_DNA-bd.
[Graphical view]
PfamPF00170. bZIP_1. 1 hit.
PF03957. Jun. 1 hit.
[Graphical view]
PRINTSPR00043. LEUZIPPRJUN.
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMSSF47454. SSF47454. 1 hit.
PROSITEPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio289767.
PROP09450.
SOURCESearch...

Entry information

Entry nameJUNB_MOUSE
AccessionPrimary (citable) accession number: P09450
Secondary accession number(s): Q8C2G9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot