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Protein

C-1-tetrahydrofolate synthase, mitochondrial

Gene

MIS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei226NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi201 – 203NADPBy similarity3
Nucleotide bindingi408 – 415ATPBy similarity8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • formate-tetrahydrofolate ligase activity Source: SGD
  • methenyltetrahydrofolate cyclohydrolase activity Source: SGD
  • methylenetetrahydrofolate dehydrogenase (NADP+) activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YBR084W-MONOMER.
BRENDAi3.5.4.9. 984.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
C-1-tetrahydrofolate synthase, mitochondrial
Short name:
C1-THF synthase
Including the following 3 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Formyltetrahydrofolate synthetase (EC:6.3.4.3)
Gene namesi
Name:MIS1
Ordered Locus Names:YBR084W
ORF Names:YBR0751
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR084W.
SGDiS000000288. MIS1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34Mitochondrion1 PublicationAdd BLAST34
ChainiPRO_000003405235 – 975C-1-tetrahydrofolate synthase, mitochondrialAdd BLAST941

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei346PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP09440.
PRIDEiP09440.

PTM databases

iPTMnetiP09440.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi32788. 96 interactors.
DIPiDIP-6724N.
IntActiP09440. 51 interactors.
MINTiMINT-660665.

Structurei

3D structure databases

ProteinModelPortaliP09440.
SMRiP09440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 343Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd BLAST309
Regioni83 – 87Substrate bindingBy similarity5
Regioni130 – 132Substrate bindingBy similarity3
Regioni301 – 305Substrate bindingBy similarity5
Regioni344 – 975Formyltetrahydrofolate synthetaseAdd BLAST632

Domaini

This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00860000133777.
HOGENOMiHOG000040280.
InParanoidiP09440.
KOiK00288.
OMAiDGFHRYN.
OrthoDBiEOG092C0B4M.

Family and domain databases

CDDicd00477. FTHFS. 1 hit.
Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 1 hit.
HAMAPiMF_01543. FTHFS. 1 hit.
MF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
PS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09440-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSRLSLLSN SRAFQQARWR IYRLKVSPTV HASQYHILSG RKLAQSIREK
60 70 80 90 100
ANDEIQAIKL KHPNFKPTLK IIQVGARPDS STYVRMKLKA SKDSNVDCII
110 120 130 140 150
EKLPAEITEV ELLKKISDIN DDDSIHGLLI QLPLPRHLDE TTITNAVDFK
160 170 180 190 200
KDVDGFHRYN AGELAKKGGK PYFIPCTPYG CMKLLEEAHV KLDGKNAVVL
210 220 230 240 250
GRSSIVGNPI ASLLKNANAT VTVCHSHTRN IAEVVSQADI VIAACGIPQY
260 270 280 290 300
VKSDWIKEGA VVIDVGINYV PDISKKSGQK LVGDVDFDSV KEKTSYITPV
310 320 330 340 350
PGGVGPMTVA MLVSNVLLAA KRQFVESEKL PVIKPLPLHL ESPVPSDIDI
360 370 380 390 400
SRAQSPKHIK QVAEELGIHS HELELYGHYK AKISPNIFKR LESRENGKYV
410 420 430 440 450
LVAGITPTPL GEGKSTTTMG LVQALSAHLG KPSIANVRQP SLGPTLGVKG
460 470 480 490 500
GAAGGGYAQV IPMDEFNLHL TGDIHAISAA NNLLAAAIDT RMFHEATQKN
510 520 530 540 550
DSTFYKRLVP RKKGIRKFTP SMQRRLKRLD IEKEDPDALT PEEVKRFARL
560 570 580 590 600
NINPDTITIR RVVDINDRML RQITIGEAAT EKGFTRTTGF DITVASELMA
610 620 630 640 650
ILALSKSLHE MKERIGRMVI GADYDNKPVT VEDIGCTGAL TALLRDAIKP
660 670 680 690 700
NLMQTLEGTP VMVHAGPFAN ISIGASSVIA DLMALKLVGS EKNPLNDKNI
710 720 730 740 750
HEPGYVVTEA GFDFAMGGER FFDIKCRSSG LVPDAVVLVA TVRALKSHGG
760 770 780 790 800
APNVKPGQSL PKEYTEENID FVAKGVSNLV KQIENIKTFG IPVVVAINRF
810 820 830 840 850
ETDSQAEIEV IKKAALNAGA SHAVTSNHWM EGGKGAVELA HAVVDATKEP
860 870 880 890 900
KNFNFLYDVN SSIEDKLTSI VQKMYGGAKI EVSPEAQKKI DTYKKQGFGN
910 920 930 940 950
LPICIAKTQY SLSHDPSLKG VPRGFTFPIR DVRASIGAGY LYALAAEIQT
960 970
IPGLSTYAGY MAVEVDDDGE IEGLF
Length:975
Mass (Da):106,217
Last modified:July 1, 1989 - v1
Checksum:iB9421D4F8981531F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35953 Genomic DNA. Translation: CAA85029.1.
J03724 Genomic DNA. Translation: AAA34781.1.
BK006936 Genomic DNA. Translation: DAA07203.1.
PIRiA28174.
RefSeqiNP_009640.1. NM_001178432.1.

Genome annotation databases

EnsemblFungiiYBR084W; YBR084W; YBR084W.
GeneIDi852378.
KEGGisce:YBR084W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35953 Genomic DNA. Translation: CAA85029.1.
J03724 Genomic DNA. Translation: AAA34781.1.
BK006936 Genomic DNA. Translation: DAA07203.1.
PIRiA28174.
RefSeqiNP_009640.1. NM_001178432.1.

3D structure databases

ProteinModelPortaliP09440.
SMRiP09440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32788. 96 interactors.
DIPiDIP-6724N.
IntActiP09440. 51 interactors.
MINTiMINT-660665.

PTM databases

iPTMnetiP09440.

Proteomic databases

MaxQBiP09440.
PRIDEiP09440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR084W; YBR084W; YBR084W.
GeneIDi852378.
KEGGisce:YBR084W.

Organism-specific databases

EuPathDBiFungiDB:YBR084W.
SGDiS000000288. MIS1.

Phylogenomic databases

GeneTreeiENSGT00860000133777.
HOGENOMiHOG000040280.
InParanoidiP09440.
KOiK00288.
OMAiDGFHRYN.
OrthoDBiEOG092C0B4M.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciYEAST:YBR084W-MONOMER.
BRENDAi3.5.4.9. 984.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.

Miscellaneous databases

PROiP09440.

Family and domain databases

CDDicd00477. FTHFS. 1 hit.
Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 1 hit.
HAMAPiMF_01543. FTHFS. 1 hit.
MF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
PS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC1TM_YEAST
AccessioniPrimary (citable) accession number: P09440
Secondary accession number(s): D6VQ83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.