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P09440

- C1TM_YEAST

UniProt

P09440 - C1TM_YEAST

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Protein
C-1-tetrahydrofolate synthase, mitochondrial
Gene
MIS1, YBR084W, YBR0751
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei226 – 2261NADP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi201 – 2033NADP By similarity
Nucleotide bindingi408 – 4158ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. formate-tetrahydrofolate ligase activity Source: SGD
  3. methenyltetrahydrofolate cyclohydrolase activity Source: SGD
  4. methylenetetrahydrofolate dehydrogenase (NADP+) activity Source: SGD

GO - Biological processi

  1. folic acid biosynthetic process Source: SGD
  2. histidine biosynthetic process Source: UniProtKB-KW
  3. methionine biosynthetic process Source: UniProtKB-KW
  4. nucleobase-containing compound metabolic process Source: SGD
  5. purine nucleotide biosynthetic process Source: UniProtKB-KW
  6. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YBR084W-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
C-1-tetrahydrofolate synthase, mitochondrial
Short name:
C1-THF synthase
Including the following 3 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Formyltetrahydrofolate synthetase (EC:6.3.4.3)
Gene namesi
Name:MIS1
Ordered Locus Names:YBR084W
ORF Names:YBR0751
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR084w.
SGDiS000000288. MIS1.

Subcellular locationi

Mitochondrion 3 Publications

GO - Cellular componenti

  1. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434Mitochondrion1 Publication
Add
BLAST
Chaini35 – 975941C-1-tetrahydrofolate synthase, mitochondrialUniRule annotation
PRO_0000034052Add
BLAST

Proteomic databases

MaxQBiP09440.
PaxDbiP09440.
PeptideAtlasiP09440.
PRIDEiP09440.

Expressioni

Gene expression databases

GenevestigatoriP09440.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RPB5P204341EBI-3903,EBI-15781
SAT4P253331EBI-3903,EBI-16492
YHR080CP388001EBI-3903,EBI-24597

Protein-protein interaction databases

BioGridi32788. 95 interactions.
DIPiDIP-6724N.
IntActiP09440. 50 interactions.
MINTiMINT-660665.
STRINGi4932.YBR084W.

Structurei

3D structure databases

ProteinModelPortaliP09440.
SMRiP09440. Positions 37-326, 344-975.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 343309Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseUniRule annotation
Add
BLAST
Regioni83 – 875Substrate binding By similarity
Regioni130 – 1323Substrate binding By similarity
Regioni301 – 3055Substrate binding By similarity
Regioni344 – 975632Formyltetrahydrofolate synthetaseUniRule annotation
Add
BLAST

Domaini

This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0190.
GeneTreeiENSGT00750000117947.
HOGENOMiHOG000040280.
KOiK00288.
OMAiLPTHAGF.
OrthoDBiEOG7K0ZMJ.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 1 hit.
HAMAPiMF_01543. FTHFS.
MF_01576. THF_DHG_CYH.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
PS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09440-1 [UniParc]FASTAAdd to Basket

« Hide

MLSRLSLLSN SRAFQQARWR IYRLKVSPTV HASQYHILSG RKLAQSIREK    50
ANDEIQAIKL KHPNFKPTLK IIQVGARPDS STYVRMKLKA SKDSNVDCII 100
EKLPAEITEV ELLKKISDIN DDDSIHGLLI QLPLPRHLDE TTITNAVDFK 150
KDVDGFHRYN AGELAKKGGK PYFIPCTPYG CMKLLEEAHV KLDGKNAVVL 200
GRSSIVGNPI ASLLKNANAT VTVCHSHTRN IAEVVSQADI VIAACGIPQY 250
VKSDWIKEGA VVIDVGINYV PDISKKSGQK LVGDVDFDSV KEKTSYITPV 300
PGGVGPMTVA MLVSNVLLAA KRQFVESEKL PVIKPLPLHL ESPVPSDIDI 350
SRAQSPKHIK QVAEELGIHS HELELYGHYK AKISPNIFKR LESRENGKYV 400
LVAGITPTPL GEGKSTTTMG LVQALSAHLG KPSIANVRQP SLGPTLGVKG 450
GAAGGGYAQV IPMDEFNLHL TGDIHAISAA NNLLAAAIDT RMFHEATQKN 500
DSTFYKRLVP RKKGIRKFTP SMQRRLKRLD IEKEDPDALT PEEVKRFARL 550
NINPDTITIR RVVDINDRML RQITIGEAAT EKGFTRTTGF DITVASELMA 600
ILALSKSLHE MKERIGRMVI GADYDNKPVT VEDIGCTGAL TALLRDAIKP 650
NLMQTLEGTP VMVHAGPFAN ISIGASSVIA DLMALKLVGS EKNPLNDKNI 700
HEPGYVVTEA GFDFAMGGER FFDIKCRSSG LVPDAVVLVA TVRALKSHGG 750
APNVKPGQSL PKEYTEENID FVAKGVSNLV KQIENIKTFG IPVVVAINRF 800
ETDSQAEIEV IKKAALNAGA SHAVTSNHWM EGGKGAVELA HAVVDATKEP 850
KNFNFLYDVN SSIEDKLTSI VQKMYGGAKI EVSPEAQKKI DTYKKQGFGN 900
LPICIAKTQY SLSHDPSLKG VPRGFTFPIR DVRASIGAGY LYALAAEIQT 950
IPGLSTYAGY MAVEVDDDGE IEGLF 975
Length:975
Mass (Da):106,217
Last modified:July 1, 1989 - v1
Checksum:iB9421D4F8981531F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z35953 Genomic DNA. Translation: CAA85029.1.
J03724 Genomic DNA. Translation: AAA34781.1.
BK006936 Genomic DNA. Translation: DAA07203.1.
PIRiA28174.
RefSeqiNP_009640.1. NM_001178432.1.

Genome annotation databases

EnsemblFungiiYBR084W; YBR084W; YBR084W.
GeneIDi852378.
KEGGisce:YBR084W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z35953 Genomic DNA. Translation: CAA85029.1 .
J03724 Genomic DNA. Translation: AAA34781.1 .
BK006936 Genomic DNA. Translation: DAA07203.1 .
PIRi A28174.
RefSeqi NP_009640.1. NM_001178432.1.

3D structure databases

ProteinModelPortali P09440.
SMRi P09440. Positions 37-326, 344-975.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32788. 95 interactions.
DIPi DIP-6724N.
IntActi P09440. 50 interactions.
MINTi MINT-660665.
STRINGi 4932.YBR084W.

Proteomic databases

MaxQBi P09440.
PaxDbi P09440.
PeptideAtlasi P09440.
PRIDEi P09440.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR084W ; YBR084W ; YBR084W .
GeneIDi 852378.
KEGGi sce:YBR084W.

Organism-specific databases

CYGDi YBR084w.
SGDi S000000288. MIS1.

Phylogenomic databases

eggNOGi COG0190.
GeneTreei ENSGT00750000117947.
HOGENOMi HOG000040280.
KOi K00288.
OMAi LPTHAGF.
OrthoDBi EOG7K0ZMJ.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci YEAST:YBR084W-MONOMER.

Miscellaneous databases

NextBioi 971174.

Gene expression databases

Genevestigatori P09440.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
3.40.50.720. 1 hit.
HAMAPi MF_01543. FTHFS.
MF_01576. THF_DHG_CYH.
InterProi IPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view ]
Pfami PF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view ]
PRINTSi PR00085. THFDHDRGNASE.
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
PS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase."
    Shannon K.W., Rabinowitz J.C.
    J. Biol. Chem. 263:7717-7725(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Purification and characterization of a mitochondrial isozyme of C1-tetrahydrofolate synthase from Saccharomyces cerevisiae."
    Shannon K.W., Rabinowitz J.C.
    J. Biol. Chem. 261:12266-12271(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 35-74, CHARACTERIZATION, SUBCELLULAR LOCATION, SUBUNIT.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiC1TM_YEAST
AccessioniPrimary (citable) accession number: P09440
Secondary accession number(s): D6VQ83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 14, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11400 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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