Reviewed, UniProtKB/Swiss-Prot P09439 (LOX2_SOYBN)

Names and origin

Protein names

Recommended name:
    Seed lipoxygenase-2
      Short name=L-2
    EC=1.13.11.12

Gene names

Name:	LOX1.2
Synonyms:	LOX2

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	865 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
Catalytic activity	Linoleate + O2 = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Miscellaneous	Soybean contains at least 4 distinct isoenzymes, L-1, L-2, L-3a and L-3b in dry seeds, and at least two distinct isozymes in the hypocotyl/radicle region of the seedling stem.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.
Sequence caution	The sequence described in Ref.2 differs from that shown. Reason: Frameshift at positions 691 and 865.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 865	865	Seed lipoxygenase-2		PRO_0000220718
Regions
	Domain	50 – 175	126	PLAT
	Domain	178 – 865	688	Lipoxygenase
Sites
	Metal binding	527	1	Iron; catalytic By similarity
	Metal binding	532	1	Iron; catalytic By similarity
	Metal binding	718	1	Iron; catalytic By similarity
	Metal binding	722	1	Iron; catalytic By similarity
	Metal binding	865	1	Iron; via carboxylate; catalytic By similarity
Experimental info
	Sequence conflict	263 – 264	2	KP → NL Ref.2
	Sequence conflict	313	1	D → Y Ref.2
	Sequence conflict	400	1	L → P Ref.2
	Sequence conflict	428	1	L → H Ref.2
	Sequence conflict	486	1	D → G Ref.2
	Sequence conflict	502	1	V → G Ref.2
	Sequence conflict	534	1	V → L Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P09439-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 74CBD32E3E4A0C85	FASTA	865	97,146
	10 20 30 40 50 60 MFSVPGVSGI LNRGGGHKIK GTVVLMRKNV LDFNSVADLT KGNVGGLIGT GLNVVGSTLD 70 80 90 100 110 120 NLTAFLGRSV ALQLISATKP LANGKGKVGK DTFLEGIIVS LPTLGAGESA FNIQFEWDES 130 140 150 160 170 180 MGIPGAFYIK NYMQVEFYLK SLTLEDVPNQ GTIRFVCNSW VYNTKLYKSV RIFFANHTYV 190 200 210 220 230 240 PSETPAALVG YREEELKNLR GDGKGERKEH DRIYDYDVYN DLGNPDHGEN FARPILGGSS 250 260 270 280 290 300 THPYPRRGRT GRYPTRKDQN SEKPGEVYVP RDENFGHLKS SDFLAYGIKS LSQYVLPAFE 310 320 330 340 350 360 SVFDLNFTPN EFDSFQDVRD LHEGGIKLPT EVISTIMPLP VVKELFRTDG EQVLKFPPPH 370 380 390 400 410 420 VIQVSKSAWM TDEEFAREMV AGVNPCVIRG LQEFPPKSNL DPTIYGEQTS KITADALDLD 430 440 450 460 470 480 GYTVDEALAS RRLFMLDYHD VFMPYIRRIN QTYAKAYATR TILFLRENGT LKPVAIELSL 490 500 510 520 530 540 PHPAGDLSGA VSQVILPAKE GVESTIWLLA KAYVVVNDSC YHQLMSHWLN THAVIEPFII 550 560 570 580 590 600 ATNRHLSALH PIYKLLTPHY RDTMNINALA RQSLINADGI IEKSFLPSKH SVEMSSAVYK 610 620 630 640 650 660 NWVFTDQALP ADLIKRGVAI KDPSAPHGLR LLIEDYPYAV DGLEIWAAIK TWVQEYVSLY 670 680 690 700 710 720 YARDDDVKPD SELQQWWKEA VEKGHGDLKD KPWWPKLQTI EELVEICTII IWTASALHAA 730 740 750 760 770 780 VNFGQYPYGG FILNRPTSSR RLLPEKGTPE YEEMVKSHQK AYLRTITSKF QTLVDLSVIE 790 800 810 820 830 840 ILSRHASDEV YLGQRDNPHW TSDSKALQAF QKFGNKLKEI EEKLARKNND QSLSNRLGPV 850 860 QLPYTLLHPN SEGLTCRGIP NSISI

References

[1]	"Primary structure of soybean lipoxygenase L-2." Shibata D., Steczko J., Dixon J.E., Andrews P.C., Hermodson M., Axelrod B. J. Biol. Chem. 263:6816-6821(1988) [PubMed: 2834391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Two soybean seed lipoxygenase nulls accumulate reduced levels of lipoxygenase transcripts." Start W.G., Ma Y., Polacco J.C., Hildebrand D.F., Freyer G.A., Altschuler M. Plant Mol. Biol. 7:11-23(1986) [Agricola: IND87003970] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 231-865.
[3]	"Nucleotide sequences of a soybean lipoxygenase gene and the short intergenic region between an upstream lipoxygenase gene." Shibata D., Kato T., Tanaka K. Plant Mol. Biol. 16:353-359(1991) [PubMed: 1909908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 859-865.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03211 mRNA. Translation: AAA33987.1. X56139 Genomic DNA. Translation: CAA39605.1.
PIR	DASYL1. A28161.
3D structure databases
SMR	P09439. Positions 15-865.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.12. 299.
Gene expression databases
Genevestigator	P09439.
Family and domain databases
InterPro	IPR008976. Lipase_LipOase. IPR000907. LipOase. IPR013819. LipOase_C. IPR020834. LipOase_CS. IPR020833. LipOase_Fe_BS. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX2_SOYBN

Accession

Primary (citable) accession number: P09439

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	February 9, 2010
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)