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P09437

- CYB2_HANAN

UniProt

P09437 - CYB2_HANAN

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Protein

Cytochrome b2, mitochondrial

Gene
CYB2
Organism
Hansenula anomala (Yeast) (Candida pelliculosa)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+.

Cofactori

FMN By similarity.
Binds 1 heme B (iron-protoporphyrin IX) group non-covalently per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151Iron (heme axial ligand) By similarity
Metal bindingi138 – 1381Iron (heme axial ligand) By similarity
Binding sitei208 – 2081Heme b By similarity
Binding sitei357 – 3571Heme b By similarity
Active sitei432 – 4321Proton acceptor By similarity
Binding sitei435 – 4351Substrate By similarity

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. heme binding Source: InterPro
  3. L-lactate dehydrogenase (cytochrome) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Flavoprotein, FMN, Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b2, mitochondrial (EC:1.1.2.3)
Alternative name(s):
L-lactate dehydrogenase [Cytochrome]
L-lactate ferricytochrome C oxidoreductase
Short name:
L-LCR
Gene namesi
Name:CYB2
OrganismiHansenula anomala (Yeast) (Candida pelliculosa)
Taxonomic identifieri4927 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeWickerhamomyces

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial intermembrane space Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7373MitochondrionAdd
BLAST
Chaini74 – 573500Cytochrome b2, mitochondrialPRO_0000006479Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP09437.
SMRiP09437. Positions 82-567.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 15778Cytochrome b5 heme-bindingAdd
BLAST
Domaini182 – 542361FMN hydroxy acid dehydrogenaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF01070. FMN_dh. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09437-1 [UniParc]FASTAAdd to Basket

« Hide

MFKSQLRTAT ARSSFRSLAS KLNPQRFNSS KTPLLNATRG SNRSKNSLIA    50
LAISLSAVSS SYYLYQKDKF ISADVPHWKD IELTPEIVSQ HNKKDDLWVV 100
LNGQVYDLTD FLPNHPGGQK IIIRYAGKDA TKIFVPIHPP DTIEKFIPPE 150
KHLGPLVGEF EQEEEELSDE EIDRLERIER KPPLSQMINL HDFETIARQI 200
LPPPALAYYC SAADDEVTLR ENHNAYHRIF FNPKILIDVK DVDISTEFFG 250
EKTSAPFYIS ATALAKLGHP EGEVAIAKGA GREDVVQMIS TLASCSFDEI 300
ADARIPGQQQ WYQLYVNADR SITEKAVRHA EERGMKGLFI TVDAPSLGRR 350
EKDMKMKFEA DSDVQGDDED IDRSQGASRA LSSFIDPSLS WKDIAFIKSI 400
TKMPIVIKGV QRKEDVLLAA EHGLQGVVLS NHGGRQLDYT RAPVEVLAEV 450
MPILKERGLD QKIDIFVDGG VRRGTDVLKA LCLGAKGVGL GRPFLYAMSS 500
YGDKGVTKAI QLLKDEIEMN MRLLGVNKIE ELTPELLDTR SIHNRAVPVA 550
KDYLYEQNYQ RMSGAEFRPG IED 573
Length:573
Mass (Da):64,202
Last modified:January 1, 1990 - v2
Checksum:i83EEF645C580BC8E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16051 Genomic DNA. Translation: CAA34183.1.
PIRiS06600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16051 Genomic DNA. Translation: CAA34183.1 .
PIRi S06600.

3D structure databases

ProteinModelPortali P09437.
SMRi P09437. Positions 82-567.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.120.10. 1 hit.
3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view ]
Pfami PF00173. Cyt-b5. 1 hit.
PF01070. FMN_dh. 1 hit.
[Graphical view ]
SUPFAMi SSF55856. SSF55856. 1 hit.
PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the Hansenula anomala gene encoding flavocytochrome b2 (L-lactate:cytochrome c oxidoreductase)."
    Risler Y., Tegoni M., Gervais M.
    Nucleic Acids Res. 17:8381-8381(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structural basis for the kinetic differences between flavocytochromes b2 from the yeasts Hansenula anomala and Saccharomyces cerevisiae."
    Black M.T., Gunn F.J., Chapman S.K., Reid G.A.
    Biochem. J. 263:973-976(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Amino-acid sequence of the cytochrome-b5-like heme-binding domain from Hansenula anomala flavocytochrome b2."
    Haumont P.-Y., Thomas M.-A., Labeyrie F., Lederer F.
    Eur. J. Biochem. 169:539-546(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 80-163.

Entry informationi

Entry nameiCYB2_HANAN
AccessioniPrimary (citable) accession number: P09437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 1, 1990
Last modified: October 16, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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