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Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

ILS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei605 – 6051ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: SGD

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28968-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ILS1
Ordered Locus Names:YBL076C
ORF Names:YBL0734
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL076c.
SGDiS000000172. ILS1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10721072Isoleucine--tRNA ligase, cytoplasmicPRO_0000098604Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei829 – 8291Phosphoserine1 Publication
Modified residuei1059 – 10591Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP09436.
PaxDbiP09436.
PeptideAtlasiP09436.
PRIDEiP09436.

Expressioni

Gene expression databases

GenevestigatoriP09436.

Interactioni

Protein-protein interaction databases

BioGridi32626. 38 interactions.
DIPiDIP-6388N.
IntActiP09436. 10 interactions.
MINTiMINT-628035.
STRINGi4932.YBL076C.

Structurei

3D structure databases

ProteinModelPortaliP09436.
SMRiP09436. Positions 37-784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074921.
HOGENOMiHOG000246403.
InParanoidiP09436.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG7FNCGS.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09436-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSESNAHFSF PKEEEKVLSL WDEIDAFHTS LELTKDKPEF SFFDGPPFAT
60 70 80 90 100
GTPHYGHILA STIKDIVPRY ATMTGHHVER RFGWDTHGVP IEHIIDKKLG
110 120 130 140 150
ITGKDDVFKY GLENYNNECR SIVMTYASDW RKTIGRLGRW IDFDNDYKTM
160 170 180 190 200
YPSFMESTWW AFKQLHEKGQ VYRGFKVMPY STGLTTPLSN FEAQQNYKDV
210 220 230 240 250
NDPAVTIGFN VIGQEKTQLV AWTTTPWTLP SNLSLCVNAD FEYVKIYDET
260 270 280 290 300
RDRYFILLES LIKTLYKKPK NEKYKIVEKI KGSDLVGLKY EPLFPYFAEQ
310 320 330 340 350
FHETAFRVIS DDYVTSDSGT GIVHNAPAFG EEDNAACLKN GVISEDSVLP
360 370 380 390 400
NAIDDLGRFT KDVPDFEGVY VKDADKLIIK YLTNTGNLLL ASQIRHSYPF
410 420 430 440 450
CWRSDTPLLY RSVPAWFVRV KNIVPQMLDS VMKSHWVPNT IKEKRFANWI
460 470 480 490 500
ANARDWNVSR NRYWGTPIPL WVSDDFEEVV CVGSIKELEE LTGVRNITDL
510 520 530 540 550
HRDVIDKLTI PSKQGKGDLK RIEEVFDCWF ESGSMPYASQ HYPFENTEKF
560 570 580 590 600
DERVPANFIS EGLDQTRGWF YTLAVLGTHL FGSVPYKNVI VSGIVLAADG
610 620 630 640 650
RKMSKSLKNY PDPSIVLNKY GADALRLYLI NSPVLKAESL KFKEEGVKEV
660 670 680 690 700
VSKVLLPWWN SFKFLDGQIA LLKKMSNIDF QYDDSVKSDN VMDRWILASM
710 720 730 740 750
QSLVQFIHEE MGQYKLYTVV PKLLNFIDEL TNWYIRFNRR RLKGENGVED
760 770 780 790 800
CLKALNSLFD ALFTFVRAMA PFTPFLSESI YLRLKEYIPE AVLAKYGKDG
810 820 830 840 850
RSVHFLSYPV VKKEYFDEAI ETAVSRMQSV IDLGRNIREK KTISLKTPLK
860 870 880 890 900
TLVILHSDES YLKDVEALKN YIIEELNVRD VVITSDEAKY GVEYKAVADW
910 920 930 940 950
PVLGKKLKKD AKKVKDALPS VTSEQVREYL ESGKLEVAGI ELVKGDLNAI
960 970 980 990 1000
RGLPESAVQA GQETRTDQDV LIIMDTNIYS ELKSEGLARE LVNRIQKLRK
1010 1020 1030 1040 1050
KCGLEATDDV LVEYELVKDT IDFEAIVKEH FDMLSKTCRS DIAKYDGSKT
1060 1070
DPIGDEEQSI NDTIFKLKVF KL
Length:1,072
Mass (Da):122,984
Last modified:July 1, 1989 - v1
Checksum:i75E07F3B0A4DBBFF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti551 – 5511D → E in AAA34712. (PubMed:3311074)Curated
Sequence conflicti588 – 5881N → KS AA sequence (PubMed:3311074)Curated
Sequence conflicti693 – 6953DRW → AEM in AAA34712. (PubMed:3311074)Curated
Sequence conflicti706 – 7061F → V in AAA34712. (PubMed:3311074)Curated
Sequence conflicti747 – 7471G → V in AAA34712. (PubMed:3311074)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07886 Genomic DNA. Translation: CAA30733.1.
M19992 Genomic DNA. Translation: AAA34712.1.
X79489 Genomic DNA. Translation: CAA56034.1.
Z35838 Genomic DNA. Translation: CAA84898.1.
BK006936 Genomic DNA. Translation: DAA07046.1.
PIRiS14459. SYBYI4.
RefSeqiNP_009477.1. NM_001178316.1.

Genome annotation databases

EnsemblFungiiYBL076C; YBL076C; YBL076C.
GeneIDi852202.
KEGGisce:YBL076C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07886 Genomic DNA. Translation: CAA30733.1.
M19992 Genomic DNA. Translation: AAA34712.1.
X79489 Genomic DNA. Translation: CAA56034.1.
Z35838 Genomic DNA. Translation: CAA84898.1.
BK006936 Genomic DNA. Translation: DAA07046.1.
PIRiS14459. SYBYI4.
RefSeqiNP_009477.1. NM_001178316.1.

3D structure databases

ProteinModelPortaliP09436.
SMRiP09436. Positions 37-784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32626. 38 interactions.
DIPiDIP-6388N.
IntActiP09436. 10 interactions.
MINTiMINT-628035.
STRINGi4932.YBL076C.

Proteomic databases

MaxQBiP09436.
PaxDbiP09436.
PeptideAtlasiP09436.
PRIDEiP09436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL076C; YBL076C; YBL076C.
GeneIDi852202.
KEGGisce:YBL076C.

Organism-specific databases

CYGDiYBL076c.
SGDiS000000172. ILS1.

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074921.
HOGENOMiHOG000246403.
InParanoidiP09436.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG7FNCGS.

Enzyme and pathway databases

BioCyciYEAST:G3O-28968-MONOMER.

Miscellaneous databases

NextBioi970697.
PROiP09436.

Gene expression databases

GenevestigatoriP09436.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)."
    Martindale D.W., Gu Z.M., Csank C.
    Curr. Genet. 15:99-106(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204626 / S288c / A364A.
  2. "Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1). DNA-sequence, amino-acid sequence of proteolytic peptides of the enzyme and comparison of the structure to those of other known aminoacyl-tRNA synthetases."
    Englisch U., Englisch E., Markmeyer P., Schischkoff J., Sternbach H., Kratzin H., Cramer F.
    Biol. Chem. Hoppe-Seyler 368:971-979(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 204508 / S288c.
  3. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
    Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
    Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-829 AND SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYIC_YEAST
AccessioniPrimary (citable) accession number: P09436
Secondary accession number(s): D6VPS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 7, 2015
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 23300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.