Reviewed, UniProtKB/Swiss-Prot P09436 (SYIC_YEAST)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ILS1
Ordered Locus Names:	YBL076C
ORF Names:	YBL0734

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1072 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm.
Miscellaneous	Present with 23300 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from direct assay. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct isoleucine-tRNA ligase activity Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-18718,EBI-18718

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1072	1072	Isoleucyl-tRNA synthetase, cytoplasmic		PRO_0000098604
Regions
	Motif	47 – 57	11	"HIGH" region
	Motif	602 – 606	5	"KMSKS" region
Sites
	Binding site	605	1	ATP By similarity
Amino acid modifications
	Modified residue	186	1	Phosphothreonine Ref.7
	Modified residue	406	1	Phosphothreonine Ref.7
	Modified residue	829	1	Phosphoserine Ref.7
	Modified residue	1059	1	Phosphoserine Ref.7 Ref.6
Experimental info
	Sequence conflict	551	1	D → E in AAA34712. Ref.2
	Sequence conflict	588	1	N → KS AA sequence Ref.2
	Sequence conflict	693 – 695	3	DRW → AEM in AAA34712. Ref.2
	Sequence conflict	706	1	F → V in AAA34712. Ref.2
	Sequence conflict	747	1	G → V in AAA34712. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P09436-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 75E07F3B0A4DBBFF	FASTA	1,072	122,984
	10 20 30 40 50 60 MSESNAHFSF PKEEEKVLSL WDEIDAFHTS LELTKDKPEF SFFDGPPFAT GTPHYGHILA 70 80 90 100 110 120 STIKDIVPRY ATMTGHHVER RFGWDTHGVP IEHIIDKKLG ITGKDDVFKY GLENYNNECR 130 140 150 160 170 180 SIVMTYASDW RKTIGRLGRW IDFDNDYKTM YPSFMESTWW AFKQLHEKGQ VYRGFKVMPY 190 200 210 220 230 240 STGLTTPLSN FEAQQNYKDV NDPAVTIGFN VIGQEKTQLV AWTTTPWTLP SNLSLCVNAD 250 260 270 280 290 300 FEYVKIYDET RDRYFILLES LIKTLYKKPK NEKYKIVEKI KGSDLVGLKY EPLFPYFAEQ 310 320 330 340 350 360 FHETAFRVIS DDYVTSDSGT GIVHNAPAFG EEDNAACLKN GVISEDSVLP NAIDDLGRFT 370 380 390 400 410 420 KDVPDFEGVY VKDADKLIIK YLTNTGNLLL ASQIRHSYPF CWRSDTPLLY RSVPAWFVRV 430 440 450 460 470 480 KNIVPQMLDS VMKSHWVPNT IKEKRFANWI ANARDWNVSR NRYWGTPIPL WVSDDFEEVV 490 500 510 520 530 540 CVGSIKELEE LTGVRNITDL HRDVIDKLTI PSKQGKGDLK RIEEVFDCWF ESGSMPYASQ 550 560 570 580 590 600 HYPFENTEKF DERVPANFIS EGLDQTRGWF YTLAVLGTHL FGSVPYKNVI VSGIVLAADG 610 620 630 640 650 660 RKMSKSLKNY PDPSIVLNKY GADALRLYLI NSPVLKAESL KFKEEGVKEV VSKVLLPWWN 670 680 690 700 710 720 SFKFLDGQIA LLKKMSNIDF QYDDSVKSDN VMDRWILASM QSLVQFIHEE MGQYKLYTVV 730 740 750 760 770 780 PKLLNFIDEL TNWYIRFNRR RLKGENGVED CLKALNSLFD ALFTFVRAMA PFTPFLSESI 790 800 810 820 830 840 YLRLKEYIPE AVLAKYGKDG RSVHFLSYPV VKKEYFDEAI ETAVSRMQSV IDLGRNIREK 850 860 870 880 890 900 KTISLKTPLK TLVILHSDES YLKDVEALKN YIIEELNVRD VVITSDEAKY GVEYKAVADW 910 920 930 940 950 960 PVLGKKLKKD AKKVKDALPS VTSEQVREYL ESGKLEVAGI ELVKGDLNAI RGLPESAVQA 970 980 990 1000 1010 1020 GQETRTDQDV LIIMDTNIYS ELKSEGLARE LVNRIQKLRK KCGLEATDDV LVEYELVKDT 1030 1040 1050 1060 1070 IDFEAIVKEH FDMLSKTCRS DIAKYDGSKT DPIGDEEQSI NDTIFKLKVF KL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)." Martindale D.W., Gu Z.M., Csank C. Curr. Genet. 15:99-106(1989) [PubMed: 2663194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204626 / S288c / A364A.
[2]	"Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1). DNA-sequence, amino-acid sequence of proteolytic peptides of the enzyme and comparison of the structure to those of other known aminoacyl-tRNA synthetases." Englisch U., Englisch E., Markmeyer P., Schischkoff J., Sternbach H., Kratzin H., Cramer F. Biol. Chem. Hoppe-Seyler 368:971-979(1987) [PubMed: 3311074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 204508 / S288c.
[3]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1059, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186; THR-406; SER-829 AND SER-1059, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07886 Genomic DNA. Translation: CAA30733.1. M19992 Genomic DNA. Translation: AAA34712.1. X79489 Genomic DNA. Translation: CAA56034.1. Z35838 Genomic DNA. Translation: CAA84898.1.
PIR	SYBYI4. S14459.
RefSeq	NP_009477.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6388N.
IntAct	P09436. 21 interactions.
STRING	P09436.
Proteomic databases
PeptideAtlas	P09436.
PRIDE	P09436.
Genome annotation databases
Ensembl	YBL076C; YBL076C; YBL076C; Saccharomyces cerevisiae. [Genome view]
GeneID	852202.
KEGG	sce:YBL076C.
NMPDR	fig|4932.3.peg.163.
Organism-specific databases
CYGD	YBL076c.
SGD	S000000172. ILS1.
Phylogenomic databases
HOGENOM	P09436.
OMA	KENGAFT
OrthoDB	EOG90VX67
Enzyme and pathway databases
BRENDA	6.1.1.5. 250.
Gene expression databases
ArrayExpress	P09436.
Genevestigator	P09436.
GermOnline	YBL076C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970697.

Entry information

Entry name

SYIC_YEAST

Accession

Primary (citable) accession number: P09436

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 24, 2009
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)