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P09436

- SYIC_YEAST

UniProt

P09436 - SYIC_YEAST

Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

ILS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei605 – 6051ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: SGD

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28968-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:ILS1
    Ordered Locus Names:YBL076C
    ORF Names:YBL0734
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL076c.
    SGDiS000000172. ILS1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10721072Isoleucine--tRNA ligase, cytoplasmicPRO_0000098604Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei829 – 8291Phosphoserine1 Publication
    Modified residuei1059 – 10591Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP09436.
    PaxDbiP09436.
    PeptideAtlasiP09436.
    PRIDEiP09436.

    Expressioni

    Gene expression databases

    GenevestigatoriP09436.

    Interactioni

    Protein-protein interaction databases

    BioGridi32626. 37 interactions.
    DIPiDIP-6388N.
    IntActiP09436. 10 interactions.
    MINTiMINT-628035.
    STRINGi4932.YBL076C.

    Structurei

    3D structure databases

    ProteinModelPortaliP09436.
    SMRiP09436. Positions 37-784.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.
    GeneTreeiENSGT00550000074921.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG7FNCGS.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09436-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSESNAHFSF PKEEEKVLSL WDEIDAFHTS LELTKDKPEF SFFDGPPFAT     50
    GTPHYGHILA STIKDIVPRY ATMTGHHVER RFGWDTHGVP IEHIIDKKLG 100
    ITGKDDVFKY GLENYNNECR SIVMTYASDW RKTIGRLGRW IDFDNDYKTM 150
    YPSFMESTWW AFKQLHEKGQ VYRGFKVMPY STGLTTPLSN FEAQQNYKDV 200
    NDPAVTIGFN VIGQEKTQLV AWTTTPWTLP SNLSLCVNAD FEYVKIYDET 250
    RDRYFILLES LIKTLYKKPK NEKYKIVEKI KGSDLVGLKY EPLFPYFAEQ 300
    FHETAFRVIS DDYVTSDSGT GIVHNAPAFG EEDNAACLKN GVISEDSVLP 350
    NAIDDLGRFT KDVPDFEGVY VKDADKLIIK YLTNTGNLLL ASQIRHSYPF 400
    CWRSDTPLLY RSVPAWFVRV KNIVPQMLDS VMKSHWVPNT IKEKRFANWI 450
    ANARDWNVSR NRYWGTPIPL WVSDDFEEVV CVGSIKELEE LTGVRNITDL 500
    HRDVIDKLTI PSKQGKGDLK RIEEVFDCWF ESGSMPYASQ HYPFENTEKF 550
    DERVPANFIS EGLDQTRGWF YTLAVLGTHL FGSVPYKNVI VSGIVLAADG 600
    RKMSKSLKNY PDPSIVLNKY GADALRLYLI NSPVLKAESL KFKEEGVKEV 650
    VSKVLLPWWN SFKFLDGQIA LLKKMSNIDF QYDDSVKSDN VMDRWILASM 700
    QSLVQFIHEE MGQYKLYTVV PKLLNFIDEL TNWYIRFNRR RLKGENGVED 750
    CLKALNSLFD ALFTFVRAMA PFTPFLSESI YLRLKEYIPE AVLAKYGKDG 800
    RSVHFLSYPV VKKEYFDEAI ETAVSRMQSV IDLGRNIREK KTISLKTPLK 850
    TLVILHSDES YLKDVEALKN YIIEELNVRD VVITSDEAKY GVEYKAVADW 900
    PVLGKKLKKD AKKVKDALPS VTSEQVREYL ESGKLEVAGI ELVKGDLNAI 950
    RGLPESAVQA GQETRTDQDV LIIMDTNIYS ELKSEGLARE LVNRIQKLRK 1000
    KCGLEATDDV LVEYELVKDT IDFEAIVKEH FDMLSKTCRS DIAKYDGSKT 1050
    DPIGDEEQSI NDTIFKLKVF KL 1072
    Length:1,072
    Mass (Da):122,984
    Last modified:July 1, 1989 - v1
    Checksum:i75E07F3B0A4DBBFF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti551 – 5511D → E in AAA34712. (PubMed:3311074)Curated
    Sequence conflicti588 – 5881N → KS AA sequence (PubMed:3311074)Curated
    Sequence conflicti693 – 6953DRW → AEM in AAA34712. (PubMed:3311074)Curated
    Sequence conflicti706 – 7061F → V in AAA34712. (PubMed:3311074)Curated
    Sequence conflicti747 – 7471G → V in AAA34712. (PubMed:3311074)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07886 Genomic DNA. Translation: CAA30733.1.
    M19992 Genomic DNA. Translation: AAA34712.1.
    X79489 Genomic DNA. Translation: CAA56034.1.
    Z35838 Genomic DNA. Translation: CAA84898.1.
    BK006936 Genomic DNA. Translation: DAA07046.1.
    PIRiS14459. SYBYI4.
    RefSeqiNP_009477.1. NM_001178316.1.

    Genome annotation databases

    EnsemblFungiiYBL076C; YBL076C; YBL076C.
    GeneIDi852202.
    KEGGisce:YBL076C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07886 Genomic DNA. Translation: CAA30733.1 .
    M19992 Genomic DNA. Translation: AAA34712.1 .
    X79489 Genomic DNA. Translation: CAA56034.1 .
    Z35838 Genomic DNA. Translation: CAA84898.1 .
    BK006936 Genomic DNA. Translation: DAA07046.1 .
    PIRi S14459. SYBYI4.
    RefSeqi NP_009477.1. NM_001178316.1.

    3D structure databases

    ProteinModelPortali P09436.
    SMRi P09436. Positions 37-784.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32626. 37 interactions.
    DIPi DIP-6388N.
    IntActi P09436. 10 interactions.
    MINTi MINT-628035.
    STRINGi 4932.YBL076C.

    Proteomic databases

    MaxQBi P09436.
    PaxDbi P09436.
    PeptideAtlasi P09436.
    PRIDEi P09436.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL076C ; YBL076C ; YBL076C .
    GeneIDi 852202.
    KEGGi sce:YBL076C.

    Organism-specific databases

    CYGDi YBL076c.
    SGDi S000000172. ILS1.

    Phylogenomic databases

    eggNOGi COG0060.
    GeneTreei ENSGT00550000074921.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG7FNCGS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28968-MONOMER.

    Miscellaneous databases

    NextBioi 970697.
    PROi P09436.

    Gene expression databases

    Genevestigatori P09436.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)."
      Martindale D.W., Gu Z.M., Csank C.
      Curr. Genet. 15:99-106(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204626 / S288c / A364A.
    2. "Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1). DNA-sequence, amino-acid sequence of proteolytic peptides of the enzyme and comparison of the structure to those of other known aminoacyl-tRNA synthetases."
      Englisch U., Englisch E., Markmeyer P., Schischkoff J., Sternbach H., Kratzin H., Cramer F.
      Biol. Chem. Hoppe-Seyler 368:971-979(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 204508 / S288c.
    3. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
      Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
      Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-829 AND SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYIC_YEAST
    AccessioniPrimary (citable) accession number: P09436
    Secondary accession number(s): D6VPS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 143 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 23300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3