Isoleucine--tRNA ligase, cytoplasmic - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P09436 (SYIC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 133. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase, cytoplasmic
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ILS1
Ordered Locus Names:	YBL076C
ORF Names:	YBL0734

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1072 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm.
Miscellaneous	Present with 23300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement PubMed 10588711. Source: SGD isoleucyl-tRNA aminoacylation Inferred from direct assay PubMed 782885. Source: SGD regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytosol Inferred from direct assay PubMed 7619074. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from direct assay PubMed 7619074 PubMed 782885. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1072

1072

Isoleucine--tRNA ligase, cytoplasmic

PRO_0000098604

Regions

Motif

47 – 57

"HIGH" region

Motif

602 – 606

"KMSKS" region

Sites

Binding site

605

ATP By similarity

Amino acid modifications

Modified residue

186

Phosphothreonine Ref.8

Modified residue

406

Phosphothreonine Ref.8

Modified residue

829

Phosphoserine Ref.8

Modified residue

1059

Phosphoserine Ref.7 Ref.8

Experimental info

Sequence conflict

551

D → E in AAA34712. Ref.2

Sequence conflict

588

N → KS AA sequence Ref.2

Sequence conflict

693 – 695

DRW → AEM in AAA34712. Ref.2

Sequence conflict

706

F → V in AAA34712. Ref.2

Sequence conflict

747

G → V in AAA34712. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P09436 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 75E07F3B0A4DBBFF
Show »

FASTA

1,072

122,984

        10         20         30         40         50         60 
MSESNAHFSF PKEEEKVLSL WDEIDAFHTS LELTKDKPEF SFFDGPPFAT GTPHYGHILA 

        70         80         90        100        110        120 
STIKDIVPRY ATMTGHHVER RFGWDTHGVP IEHIIDKKLG ITGKDDVFKY GLENYNNECR 

       130        140        150        160        170        180 
SIVMTYASDW RKTIGRLGRW IDFDNDYKTM YPSFMESTWW AFKQLHEKGQ VYRGFKVMPY 

       190        200        210        220        230        240 
STGLTTPLSN FEAQQNYKDV NDPAVTIGFN VIGQEKTQLV AWTTTPWTLP SNLSLCVNAD 

       250        260        270        280        290        300 
FEYVKIYDET RDRYFILLES LIKTLYKKPK NEKYKIVEKI KGSDLVGLKY EPLFPYFAEQ 

       310        320        330        340        350        360 
FHETAFRVIS DDYVTSDSGT GIVHNAPAFG EEDNAACLKN GVISEDSVLP NAIDDLGRFT 

       370        380        390        400        410        420 
KDVPDFEGVY VKDADKLIIK YLTNTGNLLL ASQIRHSYPF CWRSDTPLLY RSVPAWFVRV 

       430        440        450        460        470        480 
KNIVPQMLDS VMKSHWVPNT IKEKRFANWI ANARDWNVSR NRYWGTPIPL WVSDDFEEVV 

       490        500        510        520        530        540 
CVGSIKELEE LTGVRNITDL HRDVIDKLTI PSKQGKGDLK RIEEVFDCWF ESGSMPYASQ 

       550        560        570        580        590        600 
HYPFENTEKF DERVPANFIS EGLDQTRGWF YTLAVLGTHL FGSVPYKNVI VSGIVLAADG 

       610        620        630        640        650        660 
RKMSKSLKNY PDPSIVLNKY GADALRLYLI NSPVLKAESL KFKEEGVKEV VSKVLLPWWN 

       670        680        690        700        710        720 
SFKFLDGQIA LLKKMSNIDF QYDDSVKSDN VMDRWILASM QSLVQFIHEE MGQYKLYTVV 

       730        740        750        760        770        780 
PKLLNFIDEL TNWYIRFNRR RLKGENGVED CLKALNSLFD ALFTFVRAMA PFTPFLSESI 

       790        800        810        820        830        840 
YLRLKEYIPE AVLAKYGKDG RSVHFLSYPV VKKEYFDEAI ETAVSRMQSV IDLGRNIREK 

       850        860        870        880        890        900 
KTISLKTPLK TLVILHSDES YLKDVEALKN YIIEELNVRD VVITSDEAKY GVEYKAVADW 

       910        920        930        940        950        960 
PVLGKKLKKD AKKVKDALPS VTSEQVREYL ESGKLEVAGI ELVKGDLNAI RGLPESAVQA 

       970        980        990       1000       1010       1020 
GQETRTDQDV LIIMDTNIYS ELKSEGLARE LVNRIQKLRK KCGLEATDDV LVEYELVKDT 

      1030       1040       1050       1060       1070 
IDFEAIVKEH FDMLSKTCRS DIAKYDGSKT DPIGDEEQSI NDTIFKLKVF KL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)." Martindale D.W., Gu Z.M., Csank C. Curr. Genet. 15:99-106(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204626 / S288c / A364A.
[2]	"Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1). DNA-sequence, amino-acid sequence of proteolytic peptides of the enzyme and comparison of the structure to those of other known aminoacyl-tRNA synthetases." Englisch U., Englisch E., Markmeyer P., Schischkoff J., Sternbach H., Kratzin H., Cramer F. Biol. Chem. Hoppe-Seyler 368:971-979(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 204508 / S288c.
[3]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1059, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186; THR-406; SER-829 AND SER-1059, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07886 Genomic DNA. Translation: CAA30733.1. M19992 Genomic DNA. Translation: AAA34712.1. X79489 Genomic DNA. Translation: CAA56034.1. Z35838 Genomic DNA. Translation: CAA84898.1. BK006936 Genomic DNA. Translation: DAA07046.1.
PIR	SYBYI4. S14459.
RefSeq	NP_009477.1. NM_001178316.1.
3D structure databases
ProteinModelPortal	P09436.
SMR	P09436. Positions 37-784.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6388N.
IntAct	P09436. 16 interactions.
MINT	MINT-628035.
STRING	4932.YBL076C.
Proteomic databases
PaxDb	P09436.
PeptideAtlas	P09436.
PRIDE	P09436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL076C; YBL076C; YBL076C.
GeneID	852202.
KEGG	sce:YBL076C.
Organism-specific databases
CYGD	YBL076c.
SGD	S000000172. ILS1.
Phylogenomic databases
eggNOG	COG0060.
GeneTree	ENSGT00550000074921.
HOGENOM	HOG000246403.
KO	K01870.
OMA	SRSRYWG.
OrthoDB	EOG4G1QQG.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28968-MONOMER.
Gene expression databases
Genevestigator	P09436.
GermOnline	YBL076C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970697.

Entry information

Entry name

SYIC_YEAST

Accession

Primary (citable) accession number: P09436
Secondary accession number(s): D6VPS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 29, 2013
This is version 133 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents