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P09436 (SYIC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase, cytoplasmic

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ILS1
Ordered Locus Names:YBL076C
ORF Names:YBL0734
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1072 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Subcellular location

Cytoplasm HAMAP-Rule MF_02003.

Miscellaneous

Present with 23300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10721072Isoleucine--tRNA ligase, cytoplasmic HAMAP-Rule MF_02003
PRO_0000098604

Regions

Motif47 – 5711"HIGH" region HAMAP-Rule MF_02003
Motif602 – 6065"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site6051ATP By similarity

Amino acid modifications

Modified residue8291Phosphoserine Ref.7
Modified residue10591Phosphoserine Ref.7 Ref.8

Experimental info

Sequence conflict5511D → E in AAA34712. Ref.2
Sequence conflict5881N → KS AA sequence Ref.2
Sequence conflict693 – 6953DRW → AEM in AAA34712. Ref.2
Sequence conflict7061F → V in AAA34712. Ref.2
Sequence conflict7471G → V in AAA34712. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09436 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 75E07F3B0A4DBBFF

FASTA1,072122,984
        10         20         30         40         50         60 
MSESNAHFSF PKEEEKVLSL WDEIDAFHTS LELTKDKPEF SFFDGPPFAT GTPHYGHILA 

        70         80         90        100        110        120 
STIKDIVPRY ATMTGHHVER RFGWDTHGVP IEHIIDKKLG ITGKDDVFKY GLENYNNECR 

       130        140        150        160        170        180 
SIVMTYASDW RKTIGRLGRW IDFDNDYKTM YPSFMESTWW AFKQLHEKGQ VYRGFKVMPY 

       190        200        210        220        230        240 
STGLTTPLSN FEAQQNYKDV NDPAVTIGFN VIGQEKTQLV AWTTTPWTLP SNLSLCVNAD 

       250        260        270        280        290        300 
FEYVKIYDET RDRYFILLES LIKTLYKKPK NEKYKIVEKI KGSDLVGLKY EPLFPYFAEQ 

       310        320        330        340        350        360 
FHETAFRVIS DDYVTSDSGT GIVHNAPAFG EEDNAACLKN GVISEDSVLP NAIDDLGRFT 

       370        380        390        400        410        420 
KDVPDFEGVY VKDADKLIIK YLTNTGNLLL ASQIRHSYPF CWRSDTPLLY RSVPAWFVRV 

       430        440        450        460        470        480 
KNIVPQMLDS VMKSHWVPNT IKEKRFANWI ANARDWNVSR NRYWGTPIPL WVSDDFEEVV 

       490        500        510        520        530        540 
CVGSIKELEE LTGVRNITDL HRDVIDKLTI PSKQGKGDLK RIEEVFDCWF ESGSMPYASQ 

       550        560        570        580        590        600 
HYPFENTEKF DERVPANFIS EGLDQTRGWF YTLAVLGTHL FGSVPYKNVI VSGIVLAADG 

       610        620        630        640        650        660 
RKMSKSLKNY PDPSIVLNKY GADALRLYLI NSPVLKAESL KFKEEGVKEV VSKVLLPWWN 

       670        680        690        700        710        720 
SFKFLDGQIA LLKKMSNIDF QYDDSVKSDN VMDRWILASM QSLVQFIHEE MGQYKLYTVV 

       730        740        750        760        770        780 
PKLLNFIDEL TNWYIRFNRR RLKGENGVED CLKALNSLFD ALFTFVRAMA PFTPFLSESI 

       790        800        810        820        830        840 
YLRLKEYIPE AVLAKYGKDG RSVHFLSYPV VKKEYFDEAI ETAVSRMQSV IDLGRNIREK 

       850        860        870        880        890        900 
KTISLKTPLK TLVILHSDES YLKDVEALKN YIIEELNVRD VVITSDEAKY GVEYKAVADW 

       910        920        930        940        950        960 
PVLGKKLKKD AKKVKDALPS VTSEQVREYL ESGKLEVAGI ELVKGDLNAI RGLPESAVQA 

       970        980        990       1000       1010       1020 
GQETRTDQDV LIIMDTNIYS ELKSEGLARE LVNRIQKLRK KCGLEATDDV LVEYELVKDT 

      1030       1040       1050       1060       1070 
IDFEAIVKEH FDMLSKTCRS DIAKYDGSKT DPIGDEEQSI NDTIFKLKVF KL 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)."
Martindale D.W., Gu Z.M., Csank C.
Curr. Genet. 15:99-106(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204626 / S288c / A364A.
[2]"Structure of the yeast isoleucyl-tRNA synthetase gene (ILS1). DNA-sequence, amino-acid sequence of proteolytic peptides of the enzyme and comparison of the structure to those of other known aminoacyl-tRNA synthetases."
Englisch U., Englisch E., Markmeyer P., Schischkoff J., Sternbach H., Kratzin H., Cramer F.
Biol. Chem. Hoppe-Seyler 368:971-979(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 204508 / S288c.
[3]"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-829 AND SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1059, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X07886 Genomic DNA. Translation: CAA30733.1.
M19992 Genomic DNA. Translation: AAA34712.1.
X79489 Genomic DNA. Translation: CAA56034.1.
Z35838 Genomic DNA. Translation: CAA84898.1.
BK006936 Genomic DNA. Translation: DAA07046.1.
PIRSYBYI4. S14459.
RefSeqNP_009477.1. NM_001178316.1.

3D structure databases

ProteinModelPortalP09436.
SMRP09436. Positions 11-784.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32626. 37 interactions.
DIPDIP-6388N.
IntActP09436. 10 interactions.
MINTMINT-628035.
STRING4932.YBL076C.

Proteomic databases

PaxDbP09436.
PeptideAtlasP09436.
PRIDEP09436.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL076C; YBL076C; YBL076C.
GeneID852202.
KEGGsce:YBL076C.

Organism-specific databases

CYGDYBL076c.
SGDS000000172. ILS1.

Phylogenomic databases

eggNOGCOG0060.
GeneTreeENSGT00550000074921.
HOGENOMHOG000246403.
KOK01870.
OMADWNLSRS.
OrthoDBEOG7FNCGS.

Enzyme and pathway databases

BioCycYEAST:G3O-28968-MONOMER.

Gene expression databases

GenevestigatorP09436.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970697.
PROP09436.

Entry information

Entry nameSYIC_YEAST
AccessionPrimary (citable) accession number: P09436
Secondary accession number(s): D6VPS6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries