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Protein

Heat shock protein SSA3

Gene

SSA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: UniProtKB-KW
  • unfolded protein binding Source: SGD

GO - Biological processi

  • misfolded or incompletely synthesized protein catabolic process Source: SGD
  • protein folding Source: SGD
  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28967-MONOMER.
ReactomeiR-SCE-3371453. Regulation of HSF1-mediated heat shock response.
R-SCE-3371568. Attenuation phase.
R-SCE-3371571. HSF1-dependent transactivation.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein SSA3
Gene namesi
Name:SSA3
Ordered Locus Names:YBL075C
ORF Names:YBL06.07, YBL0610
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL075C.
SGDiS000000171. SSA3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 649649Heat shock protein SSA3PRO_0000078387Add
BLAST

Proteomic databases

MaxQBiP09435.
PeptideAtlasiP09435.
PRIDEiP09435.

2D gel databases

UCD-2DPAGEP09435.

PTM databases

iPTMnetiP09435.

Interactioni

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi32627. 93 interactions.
DIPiDIP-2266N.
IntActiP09435. 31 interactions.
MINTiMINT-633718.

Structurei

3D structure databases

ProteinModelPortaliP09435.
SMRiP09435. Positions 4-609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

GeneTreeiENSGT00840000130142.
HOGENOMiHOG000228135.
InParanoidiP09435.
KOiK03283.
OMAiEASTDEY.
OrthoDBiEOG728916.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09435-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRAVGIDLG TTYSCVAHFS NDRVEIIAND QGNRTTPSYV AFTDTERLIG
60 70 80 90 100
DAAKNQAAIN PHNTVFDAKR LIGRKFDDPE VTTDAKHFPF KVISRDGKPV
110 120 130 140 150
VQVEYKGETK TFTPEEISSM VLSKMKETAE NYLGTTVNDA VVTVPAYFND
160 170 180 190 200
SQRQATKDAG TIAGMNVLRI INEPTAAAIA YGLDKKGRAE HNVLIFDLGG
210 220 230 240 250
GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDNRLVNHLA TEFKRKTKKD
260 270 280 290 300
ISNNQRSLRR LRTAAERAKR ALSSSSQTSI EIDSLFEGMD FYTSLTRARF
310 320 330 340 350
EELCADLFRS TLEPVEKVLK DSKLDKSQID EIVLVGGSTR IPKIQKLVSD
360 370 380 390 400
FFNGKEPNRS INPDEAVAYG AAVQAAILTG DQSTKTQDLL LLDVAPLSLG
410 420 430 440 450
IETAGGIMTK LIPRNSTIPT KKSETFSTYA DNQPGVLIQV FEGERTRTKD
460 470 480 490 500
NNLLGKFELS GIPPAPRGVP QIDVTFDIDA NGILNVSALE KGTGKSNKIT
510 520 530 540 550
ITNDKGRLSK DDIDRMVSEA EKYRADDERE AERVQAKNQL ESYAFTLKNT
560 570 580 590 600
INEASFKEKV GEDDAKRLET ASQETIDWLD ASQAASTDEY KDRQKELEGI
610 620 630 640
ANPIMTKFYG AGAGAGPGAG ESGGFPGSMP NSGATGGGED TGPTVEEVD
Length:649
Mass (Da):70,547
Last modified:October 1, 1993 - v3
Checksum:iF4B136759B7D4636
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti620 – 6201G → A in CAA30732 (PubMed:2663194).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97225 Unassigned DNA. Translation: AAC37398.1.
Z35836 Genomic DNA. Translation: CAA84896.1.
Z26879 Genomic DNA. Translation: CAA81523.1.
D90455 Genomic DNA. No translation available.
M36115 Genomic DNA. Translation: AAA35097.1.
X07886 Genomic DNA. Translation: CAA30732.1. Sequence problems.
BK006936 Genomic DNA. Translation: DAA07047.1.
PIRiS36753.
RefSeqiNP_009478.1. NM_001178315.1.

Genome annotation databases

EnsemblFungiiYBL075C; YBL075C; YBL075C.
GeneIDi852203.
KEGGisce:YBL075C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97225 Unassigned DNA. Translation: AAC37398.1.
Z35836 Genomic DNA. Translation: CAA84896.1.
Z26879 Genomic DNA. Translation: CAA81523.1.
D90455 Genomic DNA. No translation available.
M36115 Genomic DNA. Translation: AAA35097.1.
X07886 Genomic DNA. Translation: CAA30732.1. Sequence problems.
BK006936 Genomic DNA. Translation: DAA07047.1.
PIRiS36753.
RefSeqiNP_009478.1. NM_001178315.1.

3D structure databases

ProteinModelPortaliP09435.
SMRiP09435. Positions 4-609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32627. 93 interactions.
DIPiDIP-2266N.
IntActiP09435. 31 interactions.
MINTiMINT-633718.

PTM databases

iPTMnetiP09435.

2D gel databases

UCD-2DPAGEP09435.

Proteomic databases

MaxQBiP09435.
PeptideAtlasiP09435.
PRIDEiP09435.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL075C; YBL075C; YBL075C.
GeneIDi852203.
KEGGisce:YBL075C.

Organism-specific databases

EuPathDBiFungiDB:YBL075C.
SGDiS000000171. SSA3.

Phylogenomic databases

GeneTreeiENSGT00840000130142.
HOGENOMiHOG000228135.
InParanoidiP09435.
KOiK03283.
OMAiEASTDEY.
OrthoDBiEOG728916.

Enzyme and pathway databases

BioCyciYEAST:G3O-28967-MONOMER.
ReactomeiR-SCE-3371453. Regulation of HSF1-mediated heat shock response.
R-SCE-3371568. Attenuation phase.
R-SCE-3371571. HSF1-dependent transactivation.
R-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.

Miscellaneous databases

NextBioi970700.
PROiP09435.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular evolution of the HSP70 multigene family."
    Boorstein W.R., Ziegelhoffer T., Craig E.A.
    J. Mol. Evol. 38:1-17(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4."
    Logghe M., Molemans F., Fiers W., Contreras R.
    Yeast 10:1093-1100(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-628.
    Strain: ATCC 204508 / S288c.
  5. "AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae."
    Nakazawa N., Harashima S., Oshima Y.
    Mol. Cell. Biol. 11:5693-5700(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-205.
  6. "Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae."
    Boorstein W.R., Craig E.A.
    Mol. Cell. Biol. 10:3262-3267(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
  7. "Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)."
    Martindale D.W., Gu Z.M., Csank C.
    Curr. Genet. 15:99-106(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 602-649.
    Strain: ATCC 204626 / S288c / A364A.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHSP73_YEAST
AccessioniPrimary (citable) accession number: P09435
Secondary accession number(s): D6VPS7, P18862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1993
Last modified: May 11, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6440 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.