Reviewed, UniProtKB/Swiss-Prot P09435 (HSP73_YEAST)

Names and origin

Protein names

Recommended name:
    Heat shock protein SSA3

Gene names

Name:	SSA3
Ordered Locus Names:	YBL075C
ORF Names:	YBL0610, YBL06.07

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cytoplasm.
Miscellaneous	Present with 6440 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	SRP-dependent cotranslational protein targeting to membrane, translocation Inferred from mutant phenotype. Source: SGD protein folding Inferred from genetic interaction. Source: SGD response to stress Inferred from mutant phenotype. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from genetic interaction. Source: SGD unfolded protein binding Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Heat shock protein SSA3		PRO_0000078387
Amino acid modifications
	Modified residue	417	1	Phosphothreonine Ref.8
Experimental info
	Sequence conflict	620	1	G → A in CAA30732. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P09435-1 [UniParc]. Last modified October 1, 1993. Version 3. Checksum: F4B136759B7D4636	FASTA	649	70,547
	10 20 30 40 50 60 MSRAVGIDLG TTYSCVAHFS NDRVEIIAND QGNRTTPSYV AFTDTERLIG DAAKNQAAIN 70 80 90 100 110 120 PHNTVFDAKR LIGRKFDDPE VTTDAKHFPF KVISRDGKPV VQVEYKGETK TFTPEEISSM 130 140 150 160 170 180 VLSKMKETAE NYLGTTVNDA VVTVPAYFND SQRQATKDAG TIAGMNVLRI INEPTAAAIA 190 200 210 220 230 240 YGLDKKGRAE HNVLIFDLGG GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDNRLVNHLA 250 260 270 280 290 300 TEFKRKTKKD ISNNQRSLRR LRTAAERAKR ALSSSSQTSI EIDSLFEGMD FYTSLTRARF 310 320 330 340 350 360 EELCADLFRS TLEPVEKVLK DSKLDKSQID EIVLVGGSTR IPKIQKLVSD FFNGKEPNRS 370 380 390 400 410 420 INPDEAVAYG AAVQAAILTG DQSTKTQDLL LLDVAPLSLG IETAGGIMTK LIPRNSTIPT 430 440 450 460 470 480 KKSETFSTYA DNQPGVLIQV FEGERTRTKD NNLLGKFELS GIPPAPRGVP QIDVTFDIDA 490 500 510 520 530 540 NGILNVSALE KGTGKSNKIT ITNDKGRLSK DDIDRMVSEA EKYRADDERE AERVQAKNQL 550 560 570 580 590 600 ESYAFTLKNT INEASFKEKV GEDDAKRLET ASQETIDWLD ASQAASTDEY KDRQKELEGI 610 620 630 640 ANPIMTKFYG AGAGAGPGAG ESGGFPGSMP NSGATGGGED TGPTVEEVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular evolution of the HSP70 multigene family." Boorstein W.R., Ziegelhoffer T., Craig E.A. J. Mol. Evol. 38:1-17(1994) [PubMed: 8151709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4." Logghe M., Molemans F., Fiers W., Contreras R. Yeast 10:1093-1100(1994) [PubMed: 7992509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-628. Strain: ATCC 204508 / S288c.
[4]	"AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae." Nakazawa N., Harashima S., Oshima Y. Mol. Cell. Biol. 11:5693-5700(1991) [PubMed: 1922071] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-205.
[5]	"Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces cerevisiae." Boorstein W.R., Craig E.A. Mol. Cell. Biol. 10:3262-3267(1990) [PubMed: 2188113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
[6]	"Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)." Martindale D.W., Gu Z.M., Csank C. Curr. Genet. 15:99-106(1989) [PubMed: 2663194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 602-649. Strain: ATCC 204626 / S288c / A364A.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-417, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M97225 Unassigned DNA. Translation: AAC37398.1. Z35836 Genomic DNA. Translation: CAA84896.1. Z26879 Genomic DNA. Translation: CAA81523.1. D90455 Genomic DNA. No translation available. M36115 Genomic DNA. Translation: AAA35097.1. X07886 Genomic DNA. Translation: CAA30732.1. Sequence problems.
PIR	S36753.
RefSeq	NP_009478.1.
3D structure databases
SMR	P09435. Positions 4-551.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2266N.
IntAct	P09435. 29 interactions.
STRING	P09435.
Proteomic databases
PeptideAtlas	P09435.
PRIDE	P09435.
Genome annotation databases
Ensembl	YBL075C; YBL075C; YBL075C; Saccharomyces cerevisiae. [Genome view]
GeneID	852203.
KEGG	sce:YBL075C.
NMPDR	fig|4932.3.peg.164.
Organism-specific databases
CYGD	YBL075c.
SGD	S000000171. SSA3.
Phylogenomic databases
HOGENOM	P09435.
OMA	NTINEAS
OrthoDB	EOG99CRR1
Gene expression databases
ArrayExpress	P09435.
Genevestigator	P09435.
GermOnline	YBL075C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR001023. Hsp70. IPR013126. Hsp_70. [Graphical view]
PANTHER	PTHR19375. Hsp70. 1 hit.
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970700.

Entry information

Entry name

HSP73_YEAST

Accession

Primary (citable) accession number: P09435
Secondary accession number(s): P18862

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)