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Protein

Baseplate wedge protein gp25

Gene

25

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Baseplate protein that is part of the outer wedges of the baseplate (PubMed:15315755). Probably plays a role as a connector between the central and peripheral parts of the baseplate. Involved in the tail assembly.1 PublicationBy similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate wedge protein gp25Curated
Alternative name(s):
Outer wedge of baseplate protein
Protein Gp25
Gene namesi
Name:25
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Viral baseplate protein, Viral tail protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 132132Baseplate wedge protein gp25PRO_0000165013Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homodimer. Part of the baseplate macromolecular complex which consists of gp5, gp26, gp27, gp28, gp29 (hub); gp6, gp7, gp8, gp10, gp11, gp25, gp53 (wedge); gp9, gp12, gp48 and gp54 (PubMed:2403438, PubMed:21129200).1 Publication2 Publications

Structurei

Secondary structure

1
132
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 4020Combined sources
Helixi60 – 634Combined sources
Helixi69 – 8416Combined sources
Beta strandi88 – 9811Combined sources
Turni99 – 1024Combined sources
Beta strandi103 – 11210Combined sources
Beta strandi115 – 12612Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HRZX-ray2.47A/B1-132[»]
ProteinModelPortaliP09425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GpW/Gp25 family.Curated

Phylogenomic databases

KOiK06903.

Family and domain databases

Gene3Di3.10.450.40. 1 hit.
InterProiIPR015801. Cu_amine_oxidase_N2/3.
IPR007048. GpW/Gp25/IraD.
[Graphical view]
PfamiPF04965. GPW_gp25. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANINKLYSD IDPEMKMDWN KDVSRSLGLR SIKNSLLGII TTRKGSRPFD
60 70 80 90 100
PEFGCDLSDQ LFENMTPLTA DTVERNIESA VRNYEPRIDK LAVNVIPVYD
110 120 130
DYTLIVEIRF SVIDNPDDIE QIKLQLASSN RV
Length:132
Mass (Da):15,095
Last modified:July 1, 1989 - v1
Checksum:i16C2803EE5B12B0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05134 Genomic DNA. Translation: CAA28777.1.
M77695 Genomic DNA. Translation: AAA32549.1.
AF158101 Genomic DNA. Translation: AAD42433.1.
X04856 Genomic DNA. Translation: CAA28547.1.
PIRiJF0054. GYBPT4.
RefSeqiNP_049800.1. NC_000866.4.

Genome annotation databases

GeneIDi1258633.
KEGGivg:1258633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05134 Genomic DNA. Translation: CAA28777.1.
M77695 Genomic DNA. Translation: AAA32549.1.
AF158101 Genomic DNA. Translation: AAD42433.1.
X04856 Genomic DNA. Translation: CAA28547.1.
PIRiJF0054. GYBPT4.
RefSeqiNP_049800.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HRZX-ray2.47A/B1-132[»]
ProteinModelPortaliP09425.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258633.
KEGGivg:1258633.

Phylogenomic databases

KOiK06903.

Family and domain databases

Gene3Di3.10.450.40. 1 hit.
InterProiIPR015801. Cu_amine_oxidase_N2/3.
IPR007048. GpW/Gp25/IraD.
[Graphical view]
PfamiPF04965. GPW_gp25. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: D.
  2. "Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair gene uvsY belong to spatially and temporally overlapping transcription units."
    Gruidl M.E., Chen T.C., Gargano S., Storlazzi A., Cascino A., Mosig G.
    Virology 184:359-369(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Sequence and transcripts of the bacteriophage T4 DNA repair gene uvsY."
    Gruidl M.E., Mosig G.
    Genetics 114:1061-1079(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-132.
  5. "Structure of the bacteriophage T4 baseplate as determined by chemical cross-linking."
    Watts N.R., Coombs D.H.
    J. Virol. 64:143-154(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SUBUNIT.
  6. Cited for: REVIEW.
  7. Cited for: REVIEW ON FUNCTION.
  8. "The baseplate wedges of bacteriophage T4 spontaneously assemble into hubless baseplate-like structure in vitro."
    Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.
    J. Mol. Biol. 395:349-360(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  9. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host."
    Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V., Rossmann M.G.
    Cell 118:419-429(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF THE CONTRACTED TAIL, SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiBP25_BPT4
AccessioniPrimary (citable) accession number: P09425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 27, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.