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Protein

Baseplate wedge protein gp25

Gene

25

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Baseplate protein that is located next to the tail tube (inner baseplate). Involved in sheath assembly. The gp25-(gp6)2-gp7 module is involved in sheath contraction (PubMed:27193680).2 Publications

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate wedge protein gp25Curated
Alternative name(s):
Outer wedge of baseplate protein
Protein Gp25
Gene namesi
Name:25
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • virus tail, baseplate Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Viral baseplate protein, Viral tail protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001650131 – 132Baseplate wedge protein gp25Add BLAST132

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homodimer. Interacts with gp53 and with the (gp6)2-gp7 heterotrimeric molecule; The gp25-(gp6)2-gp7 module is involved in sheath contraction (PubMed:27193680). Part of the baseplate macromolecular complex which consists of gp5, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube).3 Publications

Structurei

Secondary structure

1132
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 41Combined sources21
Helixi59 – 63Combined sources5
Helixi69 – 84Combined sources16
Beta strandi88 – 98Combined sources11
Turni99 – 102Combined sources4
Beta strandi103 – 114Combined sources12
Beta strandi120 – 126Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IV5electron microscopy4.11BD/DG/FJ/IC/T/q1-132[»]
5IV7electron microscopy6.77BD/DF/GA/O/e/u1-132[»]
5IW9X-ray2.47A/B2-132[»]
ProteinModelPortaliP09425.
SMRiP09425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GpW/Gp25 family.Curated

Phylogenomic databases

KOiK06903.

Family and domain databases

InterProiIPR007048. GpW/Gp25/IraD.
[Graphical view]
PfamiPF04965. GPW_gp25. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANINKLYSD IDPEMKMDWN KDVSRSLGLR SIKNSLLGII TTRKGSRPFD
60 70 80 90 100
PEFGCDLSDQ LFENMTPLTA DTVERNIESA VRNYEPRIDK LAVNVIPVYD
110 120 130
DYTLIVEIRF SVIDNPDDIE QIKLQLASSN RV
Length:132
Mass (Da):15,095
Last modified:July 1, 1989 - v1
Checksum:i16C2803EE5B12B0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05134 Genomic DNA. Translation: CAA28777.1.
M77695 Genomic DNA. Translation: AAA32549.1.
AF158101 Genomic DNA. Translation: AAD42433.1.
X04856 Genomic DNA. Translation: CAA28547.1.
PIRiJF0054. GYBPT4.
RefSeqiNP_049800.1. NC_000866.4.

Genome annotation databases

GeneIDi1258633.
KEGGivg:1258633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05134 Genomic DNA. Translation: CAA28777.1.
M77695 Genomic DNA. Translation: AAA32549.1.
AF158101 Genomic DNA. Translation: AAD42433.1.
X04856 Genomic DNA. Translation: CAA28547.1.
PIRiJF0054. GYBPT4.
RefSeqiNP_049800.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IV5electron microscopy4.11BD/DG/FJ/IC/T/q1-132[»]
5IV7electron microscopy6.77BD/DF/GA/O/e/u1-132[»]
5IW9X-ray2.47A/B2-132[»]
ProteinModelPortaliP09425.
SMRiP09425.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258633.
KEGGivg:1258633.

Phylogenomic databases

KOiK06903.

Family and domain databases

InterProiIPR007048. GpW/Gp25/IraD.
[Graphical view]
PfamiPF04965. GPW_gp25. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBP25_BPT4
AccessioniPrimary (citable) accession number: P09425
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.