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Protein

Mannitol-1-phosphate 5-dehydrogenase

Gene

mtlD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi3 – 14NADBy similarityAdd BLAST12

GO - Molecular functioni

  • coenzyme binding Source: InterPro
  • mannitol-1-phosphate 5-dehydrogenase activity Source: EcoCyc

GO - Biological processi

  • mannitol catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:MANNPDEHYDROG-MONOMER.
ECOL316407:JW3574-MONOMER.
MetaCyc:MANNPDEHYDROG-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannitol-1-phosphate 5-dehydrogenase (EC:1.1.1.17)
Gene namesi
Name:mtlD
Ordered Locus Names:b3600, JW3574
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10616. mtlD.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001707031 – 382Mannitol-1-phosphate 5-dehydrogenaseAdd BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei269N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP09424.
PaxDbiP09424.
PRIDEiP09424.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4261273. 5 interactors.
DIPiDIP-10268N.
IntActiP09424. 11 interactors.
MINTiMINT-1240338.
STRINGi511145.b3600.

Structurei

3D structure databases

ProteinModelPortaliP09424.
SMRiP09424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mannitol dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4107SQ3. Bacteria.
COG0246. LUCA.
HOGENOMiHOG000271391.
InParanoidiP09424.
KOiK00009.
OMAiQHHEDPL.
PhylomeDBiP09424.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00196. Mannitol_dehydrog. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR023028. Mannitol_1_phos_5_DH.
IPR000669. Mannitol_DH.
IPR013118. Mannitol_DH_C.
IPR023027. Mannitol_DH_CS.
IPR013131. Mannitol_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01232. Mannitol_dh. 1 hit.
PF08125. Mannitol_dh_C. 1 hit.
[Graphical view]
PRINTSiPR00084. MTLDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00974. MANNITOL_DHGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKALHFGAGN IGRGFIGKLL ADAGIQLTFA DVNQVVLDAL NARHSYQVHV
60 70 80 90 100
VGETEQVDTV SGVNAVSSIG DDVVDLIAQV DLVTTAVGPV VLERIAPAIA
110 120 130 140 150
KGQVKRKEQG NESPLNIIAC ENMVRGTTQL KGHVMNALPE DAKAWVEEHV
160 170 180 190 200
GFVDSAVDRI VPPSASATND PLEVTVETFS EWIVDKTQFK GALPNIPGME
210 220 230 240 250
LTDNLMAFVE RKLFTLNTGH AITAYLGKLA GHQTIRDAIL DEKIRAVVKG
260 270 280 290 300
AMEESGAVLI KRYGFDADKH AAYIQKILGR FENPYLKDDV ERVGRQPLRK
310 320 330 340 350
LSAGDRLIKP LLGTLEYGLP HKNLIEGIAA AMHFRSEDDP QAQELAALIA
360 370 380
DKGPQAALAQ ISGLDANSEV VSEAVTAYKA MQ
Length:382
Mass (Da):41,139
Last modified:October 1, 1994 - v3
Checksum:i1AC44028C150A7B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5H → V AA sequence (PubMed:8899705).Curated1
Sequence conflicti14 – 15GF → SL AA sequence (PubMed:8899705).Curated2
Sequence conflicti86A → R (PubMed:3135464).Curated1
Sequence conflicti86A → R (PubMed:1964486).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51359 Genomic DNA. Translation: CAA35744.1.
U00039 Genomic DNA. Translation: AAB18577.1.
U00096 Genomic DNA. Translation: AAC76624.1.
AP009048 Genomic DNA. Translation: BAE77693.1.
X06794 Genomic DNA. Translation: CAA29954.1. Sequence problems.
U03845 Genomic DNA. Translation: AAA92661.1.
PIRiB65160.
RefSeqiNP_418057.1. NC_000913.3.
WP_000645439.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76624; AAC76624; b3600.
BAE77693; BAE77693; BAE77693.
GeneIDi948117.
KEGGiecj:JW3574.
eco:b3600.
PATRICi32122683. VBIEscCol129921_3718.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51359 Genomic DNA. Translation: CAA35744.1.
U00039 Genomic DNA. Translation: AAB18577.1.
U00096 Genomic DNA. Translation: AAC76624.1.
AP009048 Genomic DNA. Translation: BAE77693.1.
X06794 Genomic DNA. Translation: CAA29954.1. Sequence problems.
U03845 Genomic DNA. Translation: AAA92661.1.
PIRiB65160.
RefSeqiNP_418057.1. NC_000913.3.
WP_000645439.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP09424.
SMRiP09424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261273. 5 interactors.
DIPiDIP-10268N.
IntActiP09424. 11 interactors.
MINTiMINT-1240338.
STRINGi511145.b3600.

Proteomic databases

EPDiP09424.
PaxDbiP09424.
PRIDEiP09424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76624; AAC76624; b3600.
BAE77693; BAE77693; BAE77693.
GeneIDi948117.
KEGGiecj:JW3574.
eco:b3600.
PATRICi32122683. VBIEscCol129921_3718.

Organism-specific databases

EchoBASEiEB0611.
EcoGeneiEG10616. mtlD.

Phylogenomic databases

eggNOGiENOG4107SQ3. Bacteria.
COG0246. LUCA.
HOGENOMiHOG000271391.
InParanoidiP09424.
KOiK00009.
OMAiQHHEDPL.
PhylomeDBiP09424.

Enzyme and pathway databases

BioCyciEcoCyc:MANNPDEHYDROG-MONOMER.
ECOL316407:JW3574-MONOMER.
MetaCyc:MANNPDEHYDROG-MONOMER.

Miscellaneous databases

PROiP09424.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00196. Mannitol_dehydrog. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR023028. Mannitol_1_phos_5_DH.
IPR000669. Mannitol_DH.
IPR013118. Mannitol_DH_C.
IPR023027. Mannitol_DH_CS.
IPR013131. Mannitol_DH_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01232. Mannitol_dh. 1 hit.
PF08125. Mannitol_dh_C. 1 hit.
[Graphical view]
PRINTSiPR00084. MTLDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00974. MANNITOL_DHGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTLD_ECOLI
AccessioniPrimary (citable) accession number: P09424
Secondary accession number(s): Q2M7R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.