P09416 (MYC_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myc proto-oncogene protein

Alternative name(s):
Proto-oncogene c-Myc
Transcription factor p64

Gene names

Name:

Myc

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7 By similarity. Interacts with PIM2 By similarity. Interacts with NO66 By similarity.
Subcellular location	Nucleus › nucleoplasm By similarity. Nucleus › nucleolus By similarity.
Post-translational modification	Phosphorylated by PRKDC. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome By similarity. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC. Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence By similarity. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. Also polyubiquitinated by the DCX(TRUSS) complex By similarity.
Involvement in disease	Overexpression of C-Myc is implicated in the etiology of a variety of hematopoietic tumors.
Biotechnological use	POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Ref.4
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.
Sequence caution	The sequence AAH91699.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Disease	Proto-oncogene
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation Glycoprotein Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell apoptotic process Inferred from electronic annotation. Source: Compara DNA-dependent transcription, initiation Inferred from direct assay Ref.1. Source: RGD G0 to G1 transition Inferred from expression pattern PubMed 23119170. Source: RGD MAPK cascade Inferred from sequence or structural similarity. Source: UniProtKB activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara amino acid transport Inferred from mutant phenotype PubMed 22842522. Source: RGD apoptotic DNA fragmentation Inferred from electronic annotation. Source: Compara branching involved in ureteric bud morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB canonical Wnt receptor signaling pathway involved in positive regulation of wound healing Inferred from mutant phenotype PubMed 21975427. Source: RGD cell proliferation Inferred from mutant phenotype PubMed 20639453. Source: RGD cellular iron ion homeostasis Inferred from sequence or structural similarity. Source: UniProtKB cellular response to carbohydrate stimulus Inferred from expression pattern PubMed 20694826. Source: RGD cellular response to drug Inferred from electronic annotation. Source: Compara cellular response to estrogen stimulus Inferred from expression pattern PubMed 21980073. Source: RGD cellular response to hydrostatic pressure Inferred from expression pattern PubMed 22644739. Source: RGD cellular response to interleukin-1 Inferred from expression pattern PubMed 22706317. Source: RGD chromatin remodeling Inferred from sequence or structural similarity. Source: UniProtKB detection of mechanical stimulus involved in sensory perception of sound Inferred from electronic annotation. Source: Compara embryo development Inferred from expression pattern PubMed 23077551. Source: RGD flavonoid metabolic process Inferred from expression pattern PubMed 22528217PubMed 22819302. Source: RGD fungal-type cell wall polysaccharide metabolic process Inferred from expression pattern PubMed 22060291. Source: RGD glucose metabolic process Inferred from mutant phenotype PubMed 12480946. Source: RGD glycoprotein metabolic process Inferred from expression pattern PubMed 22445893PubMed 22639698. Source: RGD hypothalamus development Inferred from expression pattern PubMed 23291449. Source: RGD inner mitochondrial membrane organization Inferred from mutant phenotype PubMed 22629444. Source: RGD intrinsic apoptotic signaling pathway Inferred from electronic annotation. Source: Compara lactic acid secretion Inferred from mutant phenotype PubMed 22842522. Source: RGD middle ear morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of cell death Inferred from mutant phenotype PubMed 22100782. Source: RGD negative regulation of cell division Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of glucose import Inferred from mutant phenotype PubMed 22842522. Source: RGD negative regulation of monocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of protein binding Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 9924025. Source: UniProtKB pathogenesis Traceable author statement PubMed 11687580. Source: RGD pigmentation Inferred from electronic annotation. Source: Compara positive regulation of ATP biosynthetic process Inferred from mutant phenotype PubMed 22629444. Source: RGD positive regulation of B cell apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of DNA biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell cycle Inferred from mutant phenotype PubMed 22210745. Source: RGD positive regulation of cellular respiration Inferred from mutant phenotype PubMed 22842522. Source: RGD positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of epithelial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of fibroblast proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of glycolysis Inferred from mutant phenotype PubMed 22842522. Source: RGD positive regulation of metanephric cap mesenchymal cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of mitochondrial membrane potential Inferred from mutant phenotype PubMed 22629444. Source: RGD positive regulation of response to DNA damage stimulus Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 12480946. Source: RGD protein processing Inferred from electronic annotation. Source: Compara pyruvate transport Inferred from mutant phenotype PubMed 22842522. Source: RGD regulation of mitotic cell cycle Inferred from mutant phenotype PubMed 12070150. Source: RGD regulation of oxidative phosphorylation Inferred from mutant phenotype PubMed 22629444. Source: RGD regulation of telomere maintenance Inferred from sequence or structural similarity. Source: UniProtKB release of cytochrome c from mitochondria Inferred from electronic annotation. Source: Compara response to DNA damage stimulus Inferred from sequence or structural similarity. Source: UniProtKB response to alkaloid Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from expression pattern PubMed 23027806. Source: RGD response to gamma radiation Inferred from sequence or structural similarity. Source: UniProtKB response to wounding Inferred from expression pattern PubMed 20694826. Source: RGD skeletal system morphogenesis Inferred from electronic annotation. Source: Compara transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15139290. Source: RGD transformation of host cell by virus Inferred from mutant phenotype PubMed 16256070. Source: RGD
Cellular_component	axon Inferred from electronic annotation. Source: Compara cytoplasm Inferred from direct assay PubMed 9130602. Source: RGD nuclear body Inferred from electronic annotation. Source: Compara nucleolus Inferred from sequence or structural similarity. Source: UniProtKB nucleoplasm Inferred from sequence or structural similarity. Source: UniProtKB spindle Inferred from electronic annotation. Source: Compara
Molecular_function	E-box binding Inferred from sequence or structural similarity. Source: UniProtKB core promoter proximal region sequence-specific DNA binding Inferred from electronic annotation. Source: Compara double-stranded DNA binding Inferred from direct assay PubMed 15139290. Source: RGD protein complex binding Inferred from sequence or structural similarity. Source: UniProtKB sequence-specific DNA binding Inferred from direct assay PubMed 15139290PubMed 17341548. Source: RGD sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 15139290. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Myc proto-oncogene protein		PRO_0000127300
Regions
	Domain	354 – 406	53	bHLH
	Region	413 – 434	22	Leucine-zipper
	Compositional bias	34 – 37	4	Poly-Gln
	Compositional bias	89 – 92	4	Poly-Gly
	Compositional bias	218 – 223	6	Poly-Ser
Amino acid modifications
	Modified residue	6	1	Phosphoserine By similarity
	Modified residue	58	1	Phosphothreonine; by GSK3; alternate By similarity
	Modified residue	62	1	Phosphoserine; by DYRK2, GSK3 and CDK2 By similarity
	Modified residue	71	1	Phosphoserine By similarity
	Modified residue	144	1	N6-acetyllysine; by PCAF By similarity
	Modified residue	149	1	N6-acetyllysine By similarity
	Modified residue	158	1	N6-acetyllysine; by PCAF By similarity
	Modified residue	162	1	Phosphoserine By similarity
	Modified residue	275	1	N6-acetyllysine; by PCAF By similarity
	Modified residue	317	1	N6-acetyllysine; by PCAF By similarity
	Modified residue	323	1	N6-acetyllysine; by PCAF By similarity
	Modified residue	329	1	Phosphoserine; by PIM2; in vitro By similarity
	Modified residue	371	1	N6-acetyllysine; by PCAF By similarity
	Glycosylation	58	1	O-linked (GlcNAc); alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09416 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 7547DCCECF74554F	FASTA	439	48,898
	10 20 30 40 50 60 MPLNVSFANR NYDLDYDSVQ PYFICDEEEN FYHQQQQSEL QPPAPSEDIW KKFELLPTPP 70 80 90 100 110 120 LSPSRRSGLC SPSYVAVATS FSPREDDDGG GGNFSTADQL EMMTELLGGD MVNQSFICDP 130 140 150 160 170 180 DDETFIKNII IQDCMWSGFS AAAKLVSEKL ASYQAARKDS TSLSPARGHS VCSTSSLYLQ 190 200 210 220 230 240 DLTAAASECI DPSVVFPYPL NDSSSPKSCT SSDSTAFSSS SDSLLSSESS PRATPEPLVL 250 260 270 280 290 300 HEETPPTTSS DSEEEQDDEE EIDVVSVEKR QPPAKRSESG SSPSRGHSKP PHSPLVLKRC 310 320 330 340 350 360 HVSTHQHNYA APPSTRKDYP AAKRAKLDSG RVLKQISNNR KCSSPRSSDT EENDKRRTHN 370 380 390 400 410 420 VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT AYILSVQADE HKLISEKDLL 430 RKRREQLKHK LEQLRNSGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of rat c-myc and adjacent regions." Hayashi K., Makino R., Kawamura H., Arisawa A., Yoneda K. Nucleic Acids Res. 15:6419-6436(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Shull J.D., Buckles L.K. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ACI/SegHsd and Brown Norway/SsNHsd. Tissue: Spleen.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	"Direct reprogramming of rat neural precursor cells and fibroblasts into pluripotent stem cells." Chang M.Y., Kim D., Kim C.H., Kang H.C., Yang E., Moon J.I., Ko S., Park J., Park K.S., Lee K.A., Hwang D.Y., Chung Y., Lanza R., Kim K.S. PLoS ONE 5:E9838-E9838(2010) [PubMed] [Europe PMC] [Abstract] Cited for: BIOTECHNOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00396 Genomic DNA. Translation: CAA68459.2. AY679729 mRNA. Translation: AAT92511.1. AY679730 mRNA. Translation: AAT92512.1. BC091699 mRNA. Translation: AAH91699.2. Different initiation.
IPI	IPI00214655.
PIR	TVRTMC. A26801.
RefSeq	NP_036735.2. NM_012603.2.
UniGene	Rn.12072.
3D structure databases
ProteinModelPortal	P09416.
SMR	P09416. Positions 353-434.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-28140N.
MINT	MINT-4508782.
PTM databases
PhosphoSite	P09416.
Proteomic databases
PaxDb	P09416.
PRIDE	P09416.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24577.
KEGG	rno:24577.
UCSC	RGD:3130. rat.
Organism-specific databases
CTD	4609.
RGD	3130. Myc.
Phylogenomic databases
eggNOG	NOG42590.
HOGENOM	HOG000043075.
HOVERGEN	HBG000472.
InParanoid	P09416.
KO	K04377.
OrthoDB	EOG42RD7N.
Gene expression databases
ArrayExpress	P09416.
Genevestigator	P09416.
GermOnline	ENSRNOG00000004500. Rattus norvegicus.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. IPR003327. Myc-LZ. IPR002418. Tscrpt_reg_Myc. IPR012682. Tscrpt_reg_Myc_N. [Graphical view]
Pfam	PF00010. HLH. 1 hit. PF02344. Myc-LZ. 1 hit. PF01056. Myc_N. 1 hit. [Graphical view]
PIRSF	PIRSF001705. Myc_protein. 1 hit.
PRINTS	PR00044. LEUZIPPRMYC.
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603730.

Entry information

Entry name

MYC_RAT

Accession

Primary (citable) accession number: P09416
Secondary accession number(s): Q6B500

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program