Reviewed, UniProtKB/Swiss-Prot P09415 (RL18_BACST)

Names and origin

Protein names

Recommended name:
    50S ribosomal protein L18
Alternative name(s):
    BL22

Gene names

Name:

rplR

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. HAMAP MF_01337
Subunit structure	Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18 subcomplex. In B.stearothermophilus only 2 proteins, L5 and L18 have been shown to be part of this subcomplex, unlike the case in E.coli and T.thermophilus where L25 (TL5) is also found. HAMAP MF_01337
Post-translational modification	The protein, when overexpressed in E.coli, contains a phosphoserine, which is required for the protein to bind to 5S rRNA (Ref.4). It has been suggested, based solely on amino acid conservation, that this occurs on Ser-57. HAMAP MF_01337
Sequence similarities	Belongs to the ribosomal protein L18P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 120

120

50S ribosomal protein L18 HAMAP MF_01337

PRO_0000131215

Secondary structure

1

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120

Helix Strand Turn

Details...

Beta strand

30 – 36

7

Beta strand

39 – 45

7

Beta strand

53 – 57

5

Turn

61 – 63

3

Helix

71 – 88

18

Beta strand

89 – 91

3

Helix

107 – 117

11

Sequences

	Sequence		Length	Mass (Da)
	P09415-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 1E58742142882667	FASTA	120	13,472
	10 20 30 40 50 60 MITKVDRNAV RKKRHARIRK KIFGTTERPR LSVFRSNKHI YAQIIDDTKS ATIVSASTLD 70 80 90 100 110 120 KEFGLDSTNN IEAAKKVGEL VAKRALEKGI KQVVFDRGGY LYHGRVKALA DAAREAGLEF

References

[1]	"The complete amino acid sequences of the 5 S rRNA binding proteins L5 and L18 from the moderate thermophile Bacillus stearothermophilus ribosome." Kimura J., Kimura M. FEBS Lett. 210:85-90(1987) [PubMed: 3542562] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIB 8924.
[2]	"Cloning, sequencing, and overexpression of genes for ribosomal proteins from Bacillus stearothermophilus." Ramakrishnan V., Gerchman S.E. J. Biol. Chem. 266:880-885(1991) [PubMed: 1985969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Isolation and characterization of 5S RNA-protein complexes from Bacillus stearothermophilus and Escherichia coli ribosomes." Horne J.R., Erdmann V.A. Mol. Gen. Genet. 119:337-344(1972) [PubMed: 4567807] [Abstract] Cited for: ISOLATION OF 5S RRNA-PROTEIN COMPLEXES.
[4]	"Phosphorylation of ribosomal protein L18 is required for its folding and binding to 5S rRNA." Bloemink M.J., Moore P.B. Biochemistry 38:13385-13390(1999) [PubMed: 10529214] [Abstract] Cited for: PHOSPHORYLATION.
[5]	"The solution structure of ribosomal protein L18 from Bacillus stearothermophilus." Turner C.F., Moore P.B. J. Mol. Biol. 335:679-684(2004) [PubMed: 14687565] [Abstract] Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M57624 Genomic DNA. Translation: AAA22702.1. Sequence problems.

PIR

R5BS8F. B29102.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OVY	NMR	-	A	1-120	[»]

ModBase

Search...

Family and domain databases

HAMAP

MF_01337.
[Tree]

InterPro

IPR005484. Ribosomal_L18/L5.
IPR004389. Ribosomal_L18_bac.
[Graphical view]

Pfam

PF00861. Ribosomal_L18p. 1 hit.
[Graphical view]

ProDom

PD001394. Ribosomal_L18p. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00060. L18_bact. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RL18_BACST

Accession

Primary (citable) accession number: P09415

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)