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Protein

Nuclear factor 1 A-type

Gene

Nfia

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi1 – 194194CTF/NF-IPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • positive regulation of transcription, DNA-templated Source: RGD
  • response to organic cyclic compound Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor 1 A-type
Short name:
NF1-A
Short name:
Nuclear factor 1/A
Alternative name(s):
CCAAT-box-binding transcription factor
Short name:
CTF
Nuclear factor I/A
Short name:
NF-I/A
Short name:
NFI-A
TGGCA-binding protein
Gene namesi
Name:Nfia
Synonyms:Nf1l21
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3170. Nfia.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Nuclear factor 1 A-typePRO_0000100193Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei258 – 2581PhosphoserineCombined sources
Modified residuei265 – 2651PhosphoserineBy similarity
Modified residuei280 – 2801PhosphoserineCombined sources
Modified residuei287 – 2871PhosphoserineCombined sources
Modified residuei300 – 3001PhosphoserineCombined sources
Modified residuei305 – 3051PhosphoserineCombined sources
Modified residuei319 – 3191PhosphoserineCombined sources
Modified residuei360 – 3601PhosphoserineBy similarity
Modified residuei469 – 4691PhosphoserineBy similarity
Modified residuei471 – 4711PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09414.
PRIDEiP09414.

PTM databases

iPTMnetiP09414.
PhosphoSiteiP09414.

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004017.

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF/NF-I family.PROSITE-ProRule annotation
Contains 1 CTF/NF-I DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3663. Eukaryota.
ENOG410Y16G. LUCA.
HOGENOMiHOG000013028.
HOVERGENiHBG006561.
InParanoidiP09414.
KOiK09168.
PhylomeDBiP09414.

Family and domain databases

InterProiIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERiPTHR11492. PTHR11492. 1 hit.
PfamiPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
[Graphical view]
PROSITEiPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09414-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSPLCLTQD EFHPFIEALL PHVRAFAYTW FNLQARKRKY FKKHEKRMSK
60 70 80 90 100
EEERAVKDEL LSEKPEVKQK WASRLLAKLR KDIRPEYRED FVLTVTGKKP
110 120 130 140 150
PCCVLSNPDQ KGKMRRIDCL RQADKVWRLD LVMVILFKGI PLESTDGERL
160 170 180 190 200
VKSPQCSNPG LCVQPHHIGV SVKELDLYLA YFVHAADSSQ SESPSQPSDA
210 220 230 240 250
DIKDQPENGH LGFQDSFVTS GVFSVTELVR VSQTPIAAGT GPNFSLSDLE
260 270 280 290 300
SSSYYSMSPG AMRRSLPSTS STSSTKRLKS VEDEMDSPGE EPFYTGQGRS
310 320 330 340 350
PGSGSQSSGW HEVEPGMPSP TTLKKSEKSG FSSPSPSQTS SLGTAFTQHH
360 370 380 390 400
RPVITGPRAS PHATPSTLHF PTSPIIQQPG PYFSHPAIRY HPQETLKEFV
410 420 430 440 450
QLVCPDAGQQ AGQVGFLNPN GSSQGKVHNP FLPTPMLPPP PPPPMARPVP
460 470 480 490 500
LPMPDTKPPT TSTEGGAASP TSPTYSTPST SPANRFVSVG PRDPSFVNIP

QQTQSWYLG
Length:509
Mass (Da):55,976
Last modified:November 1, 1997 - v2
Checksum:i59120C7090229F87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84209 mRNA. Translation: CAA58995.1.
D78017 mRNA. Translation: BAA11203.1.
AB060652 mRNA. Translation: BAB43904.1.
X13167 mRNA. Translation: CAA31565.1.
PIRiJC5428.
RefSeqiNP_037120.1. NM_012988.2.
UniGeneiRn.10550.
Rn.216378.

Genome annotation databases

GeneIDi25492.
KEGGirno:25492.
UCSCiRGD:3170. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84209 mRNA. Translation: CAA58995.1.
D78017 mRNA. Translation: BAA11203.1.
AB060652 mRNA. Translation: BAB43904.1.
X13167 mRNA. Translation: CAA31565.1.
PIRiJC5428.
RefSeqiNP_037120.1. NM_012988.2.
UniGeneiRn.10550.
Rn.216378.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004017.

PTM databases

iPTMnetiP09414.
PhosphoSiteiP09414.

Proteomic databases

PaxDbiP09414.
PRIDEiP09414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25492.
KEGGirno:25492.
UCSCiRGD:3170. rat.

Organism-specific databases

CTDi4774.
RGDi3170. Nfia.

Phylogenomic databases

eggNOGiKOG3663. Eukaryota.
ENOG410Y16G. LUCA.
HOGENOMiHOG000013028.
HOVERGENiHBG006561.
InParanoidiP09414.
KOiK09168.
PhylomeDBiP09414.

Miscellaneous databases

PROiP09414.

Family and domain databases

InterProiIPR000647. CTF/NFI.
IPR020604. CTF/NFI_DNA-bd-dom.
IPR019739. CTF/NFI_DNA-bd_CS.
IPR019548. CTF/NFI_DNA-bd_N.
IPR003619. MAD_homology1_Dwarfin-type.
[Graphical view]
PANTHERiPTHR11492. PTHR11492. 1 hit.
PfamiPF00859. CTF_NFI. 1 hit.
PF03165. MH1. 1 hit.
PF10524. NfI_DNAbd_pre-N. 1 hit.
[Graphical view]
SMARTiSM00523. DWA. 1 hit.
[Graphical view]
PROSITEiPS00349. CTF_NFI_1. 1 hit.
PS51080. CTF_NFI_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A complex interplay of positive and negative elements is responsible for the different transcriptional activity of liver NF1 variants."
    Monaci P., Nuzzo M., Stampfli S., Tollervey D., de Simone V., Nicosia A.
    Mol. Biol. Rep. 21:147-158(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Nuclear factor 1 family proteins bind to the silencer element in the rat glutathione transferase P gene."
    Osada S., Daimon S., Ikeda T., Nishihara T., Yano K., Yamasaki M., Imagawa M.
    J. Biochem. 121:355-363(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "Identification of transcription factor in the promoter region of rat regucalcin gene: binding of nuclear factor I-A1 to TTGGC motif."
    Misawa H., Yamaguchi M.
    J. Cell. Biochem. 84:795-802(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Purification of a NF1-like DNA-binding protein from rat liver and cloning of the corresponding cDNA."
    Paonessa G., Gounari F., Frank R., Cortese R.
    EMBO J. 7:3115-3123(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-509, PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-258; SER-280; SER-287; SER-300; SER-305 AND SER-319, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNFIA_RAT
AccessioniPrimary (citable) accession number: P09414
Secondary accession number(s): Q54A99, Q63782
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.