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Protein

Phosphoglycerate kinase 1

Gene

Pgk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.By similarity

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm10358), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm3839)
  2. Phosphoglycerate kinase (Pgk1), Phosphoglycerate kinase 2 (Pgk2), Phosphoglycerate kinase 1 (Pgk1), Phosphoglycerate kinase (Pgk1)
  3. no protein annotated in this organism
  4. Gamma-enolase (Eno2), Beta-enolase (Eno3), Alpha-enolase (Eno1), Enolase 4 (Eno4)
  5. Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKLR (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKM (Pkm)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Binding sitei123SubstrateBy similarity1
Binding sitei171SubstrateBy similarity1
Binding sitei220ATPBy similarity1
Binding sitei313ATP; via carbonyl oxygenBy similarity1
Binding sitei344ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi373 – 376ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.3 3474
ReactomeiR-MMU-70171 Glycolysis
R-MMU-70263 Gluconeogenesis
SABIO-RKiP09411
UniPathwayiUPA00109; UER00185

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase 1 (EC:2.7.2.3)
Gene namesi
Name:Pgk1
Synonyms:Pgk-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:97555 Pgk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001458352 – 417Phosphoglycerate kinase 1Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei6N6-succinyllysineCombined sources1
Modified residuei11N6-acetyllysineCombined sources1
Modified residuei48N6-acetyllysine; alternateBy similarity1
Modified residuei48N6-succinyllysine; alternateCombined sources1
Modified residuei75N6-acetyllysineBy similarity1
Modified residuei76PhosphotyrosineCombined sources1
Modified residuei86N6-acetyllysineBy similarity1
Modified residuei91N6-acetyllysineCombined sources1
Modified residuei97N6-acetyllysineBy similarity1
Modified residuei131N6-acetyllysine; alternateBy similarity1
Modified residuei131N6-malonyllysine; alternateBy similarity1
Modified residuei146N6-acetyllysineBy similarity1
Modified residuei191N6-succinyllysineCombined sources1
Modified residuei196PhosphotyrosineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1
Modified residuei203PhosphoserineCombined sources1
Modified residuei267N6-acetyllysineBy similarity1
Modified residuei291N6-acetyllysineCombined sources1
Modified residuei361N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP09411
MaxQBiP09411
PaxDbiP09411
PeptideAtlasiP09411
PRIDEiP09411

2D gel databases

COMPLUYEAST-2DPAGEiP09411
REPRODUCTION-2DPAGEiIPI00555069
P09411
SWISS-2DPAGEiP09411

PTM databases

iPTMnetiP09411
PhosphoSitePlusiP09411
SwissPalmiP09411

Expressioni

Tissue specificityi

Testis, lung, brain, skeletal muscle, liver, intestine, and kidney (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000062070
CleanExiMM_PGK1
ExpressionAtlasiP09411 baseline and differential
GenevisibleiP09411 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi202133, 8 interactors
DIPiDIP-51710N
IntActiP09411, 9 interactors
MINTiP09411
STRINGi10090.ENSMUSP00000136544

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Beta strandi18 – 22Combined sources5
Beta strandi33 – 35Combined sources3
Helixi38 – 52Combined sources15
Beta strandi56 – 61Combined sources6
Turni73 – 75Combined sources3
Helixi79 – 89Combined sources11
Beta strandi93 – 95Combined sources3
Beta strandi99 – 101Combined sources3
Helixi102 – 109Combined sources8
Beta strandi115 – 118Combined sources4
Helixi122 – 124Combined sources3
Turni126 – 129Combined sources4
Beta strandi130 – 133Combined sources4
Beta strandi139 – 141Combined sources3
Helixi144 – 155Combined sources12
Beta strandi159 – 163Combined sources5
Helixi166 – 168Combined sources3
Helixi174 – 177Combined sources4
Beta strandi184 – 186Combined sources3
Helixi188 – 202Combined sources15
Beta strandi206 – 212Combined sources7
Helixi218 – 220Combined sources3
Helixi221 – 228Combined sources8
Beta strandi232 – 236Combined sources5
Helixi238 – 240Combined sources3
Helixi241 – 249Combined sources9
Helixi260 – 263Combined sources4
Helixi266 – 276Combined sources11
Beta strandi279 – 281Combined sources3
Beta strandi284 – 293Combined sources10
Beta strandi298 – 302Combined sources5
Turni303 – 305Combined sources3
Beta strandi312 – 316Combined sources5
Helixi318 – 330Combined sources13
Beta strandi332 – 338Combined sources7
Helixi346 – 348Combined sources3
Helixi350 – 364Combined sources15
Beta strandi368 – 376Combined sources9
Beta strandi389 – 394Combined sources6
Helixi396 – 403Combined sources8
Helixi409 – 412Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4O3FX-ray2.11A1-417[»]
ProteinModelPortaliP09411
SMRiP09411
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 26Substrate bindingBy similarity3
Regioni63 – 66Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiKOG1367 Eukaryota
COG0126 LUCA
GeneTreeiENSGT00390000008820
HOGENOMiHOG000227107
HOVERGENiHBG008177
InParanoidiP09411
KOiK00927
OMAiDMIFDIG
OrthoDBiEOG091G08S6
PhylomeDBiP09411
TreeFamiTF300489

Family and domain databases

CDDicd00318 Phosphoglycerate_kinase, 1 hit
Gene3Di3.40.50.1260, 4 hits
HAMAPiMF_00145 Phosphoglyc_kinase, 1 hit
InterProiView protein in InterPro
IPR001576 Phosphoglycerate_kinase
IPR015911 Phosphoglycerate_kinase_CS
IPR015824 Phosphoglycerate_kinase_N
IPR036043 Phosphoglycerate_kinase_sf
PANTHERiPTHR11406 PTHR11406, 1 hit
PfamiView protein in Pfam
PF00162 PGK, 1 hit
PIRSFiPIRSF000724 Pgk, 1 hit
PRINTSiPR00477 PHGLYCKINASE
SUPFAMiSSF53748 SSF53748, 1 hit
PROSITEiView protein in PROSITE
PS00111 PGLYCERATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC
60 70 80 90 100
LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAAELKSLLG KDVLFLKDCV
110 120 130 140 150
GPEVENACAN PAAGTVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIDAF
160 170 180 190 200
RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA
210 220 230 240 250
LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN
260 270 280 290 300
MEIGTSLYDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ
310 320 330 340 350
ATVASGIPAG WMGLDCGTES SKKYAEAVGR AKQIVWNGPV GVFEWEAFAR
360 370 380 390 400
GTKSLMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL
410
ELLEGKVLPG VDALSNV
Length:417
Mass (Da):44,550
Last modified:May 1, 2007 - v4
Checksum:i5E2EE194FF9D8CEE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56K → N in AAA70267 (PubMed:3542714).Curated1
Sequence conflicti184K → R in BAE26693 (PubMed:16141072).Curated1
Sequence conflicti265I → V in BAE37790 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15668 mRNA Translation: AAA70267.1
AK145846 mRNA Translation: BAE26693.1
AK167710 mRNA Translation: BAE39754.1
AK167459 mRNA Translation: BAE39544.1
AK167441 mRNA Translation: BAE39527.1
AK133877 mRNA Translation: BAE21906.1
AK164440 mRNA Translation: BAE37790.1
BX469914 Genomic DNA Translation: CAM17784.1
BC083355 mRNA Translation: AAH83355.1
BC108372 mRNA Translation: AAI08373.1
M18735 Genomic DNA Translation: AAA39919.1
X55309 Genomic DNA Translation: CAA39013.1
X15339 Genomic DNA Translation: CAA33391.1
CCDSiCCDS30339.1
PIRiA25567
RefSeqiNP_032854.2, NM_008828.3
UniGeneiMm.316355
Mm.336204
Mm.336205

Genome annotation databases

EnsembliENSMUST00000081593; ENSMUSP00000080302; ENSMUSG00000062070
GeneIDi18655
KEGGimmu:18655
UCSCiuc009ubo.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPGK1_MOUSE
AccessioniPrimary (citable) accession number: P09411
Secondary accession number(s): Q3TPE6, Q3UKV8, Q5XJE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 1, 2007
Last modified: March 28, 2018
This is version 163 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health