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P09411

- PGK1_MOUSE

UniProt

P09411 - PGK1_MOUSE

Protein

Phosphoglycerate kinase 1

Gene

Pgk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 4 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei39 – 391SubstrateBy similarity
    Binding sitei123 – 1231SubstrateBy similarity
    Binding sitei171 – 1711SubstrateBy similarity
    Binding sitei220 – 2201ATPBy similarity
    Binding sitei313 – 3131ATP; via carbonyl oxygenBy similarity
    Binding sitei344 – 3441ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi373 – 3764ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. phosphoglycerate kinase activity Source: UniProtKB

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    2. phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKP09411.
    UniPathwayiUPA00109; UER00185.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglycerate kinase 1 (EC:2.7.2.3)
    Gene namesi
    Name:Pgk1
    Synonyms:Pgk-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:97555. Pgk1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 417416Phosphoglycerate kinase 1PRO_0000145835Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei6 – 61N6-succinyllysine1 Publication
    Modified residuei11 – 111N6-acetyllysine1 Publication
    Modified residuei48 – 481N6-acetyllysine; alternateBy similarity
    Modified residuei48 – 481N6-succinyllysine; alternate1 Publication
    Modified residuei75 – 751N6-acetyllysineBy similarity
    Modified residuei76 – 761Phosphotyrosine1 Publication
    Modified residuei86 – 861N6-acetyllysineBy similarity
    Modified residuei91 – 911N6-acetyllysine1 Publication
    Modified residuei97 – 971N6-acetyllysineBy similarity
    Modified residuei131 – 1311N6-acetyllysine; alternateBy similarity
    Modified residuei131 – 1311N6-malonyllysine; alternateBy similarity
    Modified residuei146 – 1461N6-acetyllysineBy similarity
    Modified residuei191 – 1911N6-succinyllysine1 Publication
    Modified residuei196 – 1961PhosphotyrosineBy similarity
    Modified residuei199 – 1991N6-acetyllysineBy similarity
    Modified residuei203 – 2031Phosphoserine2 Publications
    Modified residuei267 – 2671N6-acetyllysineBy similarity
    Modified residuei291 – 2911N6-acetyllysine1 Publication
    Modified residuei361 – 3611N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP09411.
    PaxDbiP09411.
    PRIDEiP09411.

    2D gel databases

    COMPLUYEAST-2DPAGEP09411.
    REPRODUCTION-2DPAGEIPI00555069.
    P09411.
    SWISS-2DPAGEP09411.

    PTM databases

    PhosphoSiteiP09411.

    Expressioni

    Gene expression databases

    BgeeiP09411.
    CleanExiMM_PGK1.
    GenevestigatoriP09411.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi202133. 3 interactions.
    IntActiP09411. 7 interactions.
    MINTiMINT-1854650.

    Structurei

    3D structure databases

    ProteinModelPortaliP09411.
    SMRiP09411. Positions 5-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 263Substrate bindingBy similarity
    Regioni63 – 664Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the phosphoglycerate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0126.
    GeneTreeiENSGT00390000008820.
    HOGENOMiHOG000227107.
    HOVERGENiHBG008177.
    InParanoidiP09411.
    KOiK00927.
    OMAiASCCAKW.
    OrthoDBiEOG74R1QN.
    PhylomeDBiP09411.
    TreeFamiTF300489.

    Family and domain databases

    Gene3Di3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPiMF_00145. Phosphoglyc_kinase.
    InterProiIPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view]
    PANTHERiPTHR11406. PTHR11406. 1 hit.
    PfamiPF00162. PGK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000724. Pgk. 1 hit.
    PRINTSiPR00477. PHGLYCKINASE.
    SUPFAMiSSF53748. SSF53748. 1 hit.
    PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09411-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC    50
    LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAAELKSLLG KDVLFLKDCV 100
    GPEVENACAN PAAGTVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIDAF 150
    RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA 200
    LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN 250
    MEIGTSLYDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ 300
    ATVASGIPAG WMGLDCGTES SKKYAEAVGR AKQIVWNGPV GVFEWEAFAR 350
    GTKSLMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL 400
    ELLEGKVLPG VDALSNV 417
    Length:417
    Mass (Da):44,550
    Last modified:May 1, 2007 - v4
    Checksum:i5E2EE194FF9D8CEE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti56 – 561K → N in AAA70267. (PubMed:3542714)Curated
    Sequence conflicti184 – 1841K → R in BAE26693. (PubMed:16141072)Curated
    Sequence conflicti265 – 2651I → V in BAE37790. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15668 mRNA. Translation: AAA70267.1.
    AK145846 mRNA. Translation: BAE26693.1.
    AK167710 mRNA. Translation: BAE39754.1.
    AK167459 mRNA. Translation: BAE39544.1.
    AK167441 mRNA. Translation: BAE39527.1.
    AK133877 mRNA. Translation: BAE21906.1.
    AK164440 mRNA. Translation: BAE37790.1.
    BX469914 Genomic DNA. Translation: CAM17784.1.
    BC083355 mRNA. Translation: AAH83355.1.
    BC108372 mRNA. Translation: AAI08373.1.
    M18735 Genomic DNA. Translation: AAA39919.1.
    X55309 Genomic DNA. Translation: CAA39013.1.
    X15339 Genomic DNA. Translation: CAA33391.1.
    CCDSiCCDS30339.1.
    PIRiA25567.
    RefSeqiNP_032854.2. NM_008828.3.
    UniGeneiMm.316355.
    Mm.336204.
    Mm.336205.

    Genome annotation databases

    EnsembliENSMUST00000081593; ENSMUSP00000080302; ENSMUSG00000062070.
    ENSMUST00000085735; ENSMUSP00000136544; ENSMUSG00000066632.
    GeneIDi18655.
    KEGGimmu:18655.
    UCSCiuc009ubo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15668 mRNA. Translation: AAA70267.1 .
    AK145846 mRNA. Translation: BAE26693.1 .
    AK167710 mRNA. Translation: BAE39754.1 .
    AK167459 mRNA. Translation: BAE39544.1 .
    AK167441 mRNA. Translation: BAE39527.1 .
    AK133877 mRNA. Translation: BAE21906.1 .
    AK164440 mRNA. Translation: BAE37790.1 .
    BX469914 Genomic DNA. Translation: CAM17784.1 .
    BC083355 mRNA. Translation: AAH83355.1 .
    BC108372 mRNA. Translation: AAI08373.1 .
    M18735 Genomic DNA. Translation: AAA39919.1 .
    X55309 Genomic DNA. Translation: CAA39013.1 .
    X15339 Genomic DNA. Translation: CAA33391.1 .
    CCDSi CCDS30339.1.
    PIRi A25567.
    RefSeqi NP_032854.2. NM_008828.3.
    UniGenei Mm.316355.
    Mm.336204.
    Mm.336205.

    3D structure databases

    ProteinModelPortali P09411.
    SMRi P09411. Positions 5-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202133. 3 interactions.
    IntActi P09411. 7 interactions.
    MINTi MINT-1854650.

    PTM databases

    PhosphoSitei P09411.

    2D gel databases

    COMPLUYEAST-2DPAGE P09411.
    REPRODUCTION-2DPAGE IPI00555069.
    P09411.
    SWISS-2DPAGE P09411.

    Proteomic databases

    MaxQBi P09411.
    PaxDbi P09411.
    PRIDEi P09411.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081593 ; ENSMUSP00000080302 ; ENSMUSG00000062070 .
    ENSMUST00000085735 ; ENSMUSP00000136544 ; ENSMUSG00000066632 .
    GeneIDi 18655.
    KEGGi mmu:18655.
    UCSCi uc009ubo.1. mouse.

    Organism-specific databases

    CTDi 5230.
    MGIi MGI:97555. Pgk1.

    Phylogenomic databases

    eggNOGi COG0126.
    GeneTreei ENSGT00390000008820.
    HOGENOMi HOG000227107.
    HOVERGENi HBG008177.
    InParanoidi P09411.
    KOi K00927.
    OMAi ASCCAKW.
    OrthoDBi EOG74R1QN.
    PhylomeDBi P09411.
    TreeFami TF300489.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00185 .
    SABIO-RK P09411.

    Miscellaneous databases

    ChiTaRSi PGK1. mouse.
    NextBioi 294662.
    PROi P09411.
    SOURCEi Search...

    Gene expression databases

    Bgeei P09411.
    CleanExi MM_PGK1.
    Genevestigatori P09411.

    Family and domain databases

    Gene3Di 3.40.50.1260. 1 hit.
    3.40.50.1270. 1 hit.
    HAMAPi MF_00145. Phosphoglyc_kinase.
    InterProi IPR001576. Phosphoglycerate_kinase.
    IPR015901. Phosphoglycerate_kinase_C.
    IPR015911. Phosphoglycerate_kinase_CS.
    IPR015824. Phosphoglycerate_kinase_N.
    [Graphical view ]
    PANTHERi PTHR11406. PTHR11406. 1 hit.
    Pfami PF00162. PGK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000724. Pgk. 1 hit.
    PRINTSi PR00477. PHGLYCKINASE.
    SUPFAMi SSF53748. SSF53748. 1 hit.
    PROSITEi PS00111. PGLYCERATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of a cDNA clone containing the entire coding region for mouse X-chromosome-linked phosphoglycerate kinase."
      Mori N., Singer-Sam J., Lee C.-Y., Riggs A.D.
      Gene 45:275-280(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye and Placenta.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: 129 and C57BL/6.
      Tissue: Brain and Mammary tumor.
    5. "Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter."
      Adra C.N., Boer P.H., McBurney M.W.
      Gene 60:65-74(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    6. "Selective activation of testis-specific genes in cultured rat spermatogenic cells."
      Tamaru M., Nagao Y., Taira M., Tatibana M., Masamune Y., Nakanishi Y.
      Biochim. Biophys. Acta 1049:331-338(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    7. "Polymorphisms in the coding and noncoding regions of murine Pgk-1 alleles."
      Boer P.H., Potten H., Adra C.N., Jardine K., Mullhofer G., McBurney M.W.
      Biochem. Genet. 28:299-308(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
    8. Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 23-30; 76-86; 98-123; 157-184; 193-216; 247-264; 280-297; 333-350 AND 389-417, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    9. "Evolutionary conservation of the substrate-binding cleft of phosphoglycerate kinases."
      Mori N., Singer-Sam J., Riggs A.D.
      FEBS Lett. 204:313-317(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.
    10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    11. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    12. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    13. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2; LYS-11; LYS-91; LYS-291 AND LYS-361, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-6; LYS-48 AND LYS-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast and Liver.

    Entry informationi

    Entry nameiPGK1_MOUSE
    AccessioniPrimary (citable) accession number: P09411
    Secondary accession number(s): Q3TPE6, Q3UKV8, Q5XJE7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 133 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3