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Protein

Nucleolin

Gene

Ncl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolin
Alternative name(s):
Protein C23
Gene namesi
Name:Ncl
Synonyms:Nuc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97286. Ncl.

Subcellular locationi

  • Nucleusnucleolus
  • Cytoplasm By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.By similarity

GO - Cellular componenti

  • cell cortex Source: MGI
  • cytoplasmic ribonucleoprotein granule Source: MGI
  • extracellular exosome Source: MGI
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • membrane Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000816932 – 707NucleolinAdd BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9N6-acetyllysineCombined sources1
Modified residuei15N6-acetyllysineCombined sources1
Modified residuei16N6-acetyllysineCombined sources1
Modified residuei28PhosphoserineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei40PhosphoserineCombined sources1
Modified residuei41PhosphoserineCombined sources1
Modified residuei67PhosphoserineBy similarity1
Modified residuei69PhosphothreonineBy similarity1
Modified residuei76PhosphothreonineBy similarity1
Modified residuei84PhosphothreonineBy similarity1
Modified residuei92PhosphothreonineBy similarity1
Modified residuei96N6-acetyllysineCombined sources1
Modified residuei99PhosphothreonineBy similarity1
Modified residuei102N6-acetyllysineCombined sources1
Modified residuei106PhosphothreonineBy similarity1
Modified residuei109N6-acetyllysineCombined sources1
Modified residuei116N6-acetyllysineCombined sources1
Modified residuei121PhosphothreonineBy similarity1
Modified residuei124N6-acetyllysineBy similarity1
Modified residuei145PhosphoserineCombined sources1
Modified residuei157PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei220PhosphothreonineBy similarity1
Cross-linki299Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki299Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei303PhosphoserineCombined sources1
Modified residuei320N6-acetyllysineBy similarity1
Cross-linki326Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei350N6-acetyllysineCombined sources1
Modified residuei358PhosphoserineBy similarity1
Modified residuei369PhosphothreonineBy similarity1
Modified residuei379N6-acetyllysineBy similarity1
Modified residuei400N6-acetyllysineBy similarity1
Modified residuei403PhosphoserineCombined sources1
Modified residuei407PhosphothreonineBy similarity1
Modified residuei429N6-acetyllysineCombined sources1
Modified residuei446N6-acetyllysineCombined sources1
Modified residuei460PhosphoserineCombined sources1
Modified residuei462PhosphoserineBy similarity1
Modified residuei469N6-acetyllysineCombined sources1
Modified residuei478N6-acetyllysineCombined sources1
Modified residuei514N6-acetyllysineBy similarity1
Modified residuei522N6-acetyllysineCombined sources1
Modified residuei569N6-acetyllysineBy similarity1
Modified residuei574N6-acetyllysineCombined sources1
Modified residuei577PhosphoserineBy similarity1
Cross-linki586Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei588PhosphoserineBy similarity1
Modified residuei616PhosphoserineCombined sources1
Modified residuei643N6-acetyllysineCombined sources1
Modified residuei653Asymmetric dimethylarginineBy similarity1
Modified residuei657Asymmetric dimethylarginineBy similarity1
Modified residuei663Asymmetric dimethylarginineBy similarity1
Modified residuei667Asymmetric dimethylarginineBy similarity1
Modified residuei670Asymmetric dimethylarginineBy similarity1
Modified residuei691Omega-N-methylarginineCombined sources1

Post-translational modificationi

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP09405.
MaxQBiP09405.
PaxDbiP09405.
PeptideAtlasiP09405.
PRIDEiP09405.
TopDownProteomicsiP09405.

2D gel databases

REPRODUCTION-2DPAGEP09405.

PTM databases

iPTMnetiP09405.
PhosphoSitePlusiP09405.
SwissPalmiP09405.

Miscellaneous databases

PMAP-CutDBP09405.

Expressioni

Tissue specificityi

Expressed in B-cells that have been induced to switch to various Ig isotypes.1 Publication

Gene expression databases

BgeeiENSMUSG00000026234.
CleanExiMM_NCL.
ExpressionAtlasiP09405. baseline and differential.
GenevisibleiP09405. MM.

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (By similarity). Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70 (PubMed:9642267). Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin (By similarity). Interacts with AICDA (PubMed:21518874). Interacts with APTX (By similarity). Interacts with C1QBP (By similarity). Interacts with ERBB4 (By similarity). Interacts (via C-terminus) with FMR1 isoform 6 (via N-terminus) (By similarity). Interacts with GZF1; this interaction is important for nucleolar localization of GZF1 (By similarity). Interacts with NSUN2 (By similarity). Interacts with NVL (PubMed:21474449). Interacts (via N-terminus domain) with SETX (By similarity). Interacts (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains) with TERT; the interaction is important for nucleolar localization of TERT (By similarity). Interacts with WDR46 (By similarity). Interacts with ZFP36 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ankrd1Q9CR425EBI-641864,EBI-8308696
Gnai3Q9DC514EBI-641864,EBI-641852
Tpt1P630284EBI-641864,EBI-1635228

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201706. 11 interactors.
IntActiP09405. 13 interactors.
MINTiMINT-111156.
STRINGi10090.ENSMUSP00000027438.

Structurei

3D structure databases

ProteinModelPortaliP09405.
SMRiP09405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati58 – 6518
Repeati75 – 8228
Repeati83 – 9038
Repeati91 – 9848
Repeati99 – 1045; truncated6
Repeati105 – 11268
Repeati120 – 12778
Repeati128 – 13588
Domaini309 – 385RRM 1PROSITE-ProRule annotationAdd BLAST77
Domaini395 – 468RRM 2PROSITE-ProRule annotationAdd BLAST74
Domaini487 – 561RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini569 – 644RRM 4PROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 1358 X 8 AA tandem repeats of X-T-P-X-K-K-X-XAdd BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi143 – 170Asp/Glu-rich (acidic)Add BLAST28
Compositional biasi190 – 215Asp/Glu-rich (acidic)Add BLAST26
Compositional biasi241 – 273Asp/Glu-rich (acidic)Add BLAST33
Compositional biasi646 – 697Arg/Gly/Phe-richAdd BLAST52

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IRS7. Eukaryota.
ENOG410XSFV. LUCA.
GeneTreeiENSGT00840000129894.
HOGENOMiHOG000113885.
HOVERGENiHBG002295.
InParanoidiP09405.
KOiK11294.
OMAiCNKMEIE.
OrthoDBiEOG091G15MK.
PhylomeDBiP09405.
TreeFamiTF328499.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09405-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKLAKAGKT HGEAKKMAPP PKEVEEDSED EEMSEDEDDS SGEEEVVIPQ
60 70 80 90 100
KKGKKATTTP AKKVVVSQTK KAAVPTPAKK AAVTPGKKAV ATPAKKNITP
110 120 130 140 150
AKVIPTPGKK GAAQAKALVP TPGKKGAATP AKGAKNGKNA KKEDSDEDED
160 170 180 190 200
EEDEDDSDED EDDEEEDEFE PPIVKGVKPA KAAPAAPASE DEEDDEDEDD
210 220 230 240 250
EEDDDEEEED DSEEEVMEIT TAKGKKTPAK VVPMKAKSVA EEEDDEEEDE
260 270 280 290 300
DDEDEDDEEE DDEDDDEEEE EEEPVKAAPG KRKKEMTKQK EAPEAKKQKV
310 320 330 340 350
EGSEPTTPFN LFIGNLNPNK SVNELKFAIS ELFAKNDLAV VDVRTGTNRK
360 370 380 390 400
FGYVDFESAE DLEKALELTG LKVFGNEIKL EKPKGRDSKK VRAARTLLAK
410 420 430 440 450
NLSFNITEDE LKEVFEDAME IRLVSQDGKS KGIAYIEFKS EADAEKNLEE
460 470 480 490 500
KQGAEIDGRS VSLYYTGEKG QRQERTGKTS TWSGESKTLV LSNLSYSATK
510 520 530 540 550
ETLEEVFEKA TFIKVPQNPH GKPKGYAFIE FASFEDAKEA LNSCNKMEIE
560 570 580 590 600
GRTIRLELQG SNSRSQPSKT LFVKGLSEDT TEETLKESFE GSVRARIVTD
610 620 630 640 650
RETGSSKGFG FVDFNSEEDA KAAKEAMEDG EIDGNKVTLD WAKPKGEGGF
660 670 680 690 700
GGRGGGRGGF GGRGGGRGGR GGFGGRGRGG FGGRGGFRGG RGGGGDFKPQ

GKKTKFE
Length:707
Mass (Da):76,723
Last modified:January 23, 2007 - v2
Checksum:iEE2CE2ACDBF54CD4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155D → E in AAH05460 (PubMed:15489334).Curated1
Sequence conflicti203 – 204DD → EE in AAH05460 (PubMed:15489334).Curated2
Sequence conflicti245D → E in AAH05460 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07699 Genomic DNA. Translation: CAA30538.1.
AF318184 mRNA. Translation: AAK07920.1.
AK050958 mRNA. Translation: BAC34476.1.
AK083307 mRNA. Translation: BAC38858.1.
AK144894 mRNA. Translation: BAE26119.1.
AK161706 mRNA. Translation: BAE36542.1.
AK163275 mRNA. Translation: BAE37270.1.
BC005460 mRNA. Translation: AAH05460.1.
M22089 Genomic DNA. Translation: AAA39841.1.
CCDSiCCDS35646.1.
PIRiA29958. DNMS.
RefSeqiNP_035010.3. NM_010880.3.
UniGeneiMm.154378.
Mm.474153.

Genome annotation databases

EnsembliENSMUST00000027438; ENSMUSP00000027438; ENSMUSG00000026234.
GeneIDi17975.
KEGGimmu:17975.
UCSCiuc007bvl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07699 Genomic DNA. Translation: CAA30538.1.
AF318184 mRNA. Translation: AAK07920.1.
AK050958 mRNA. Translation: BAC34476.1.
AK083307 mRNA. Translation: BAC38858.1.
AK144894 mRNA. Translation: BAE26119.1.
AK161706 mRNA. Translation: BAE36542.1.
AK163275 mRNA. Translation: BAE37270.1.
BC005460 mRNA. Translation: AAH05460.1.
M22089 Genomic DNA. Translation: AAA39841.1.
CCDSiCCDS35646.1.
PIRiA29958. DNMS.
RefSeqiNP_035010.3. NM_010880.3.
UniGeneiMm.154378.
Mm.474153.

3D structure databases

ProteinModelPortaliP09405.
SMRiP09405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201706. 11 interactors.
IntActiP09405. 13 interactors.
MINTiMINT-111156.
STRINGi10090.ENSMUSP00000027438.

PTM databases

iPTMnetiP09405.
PhosphoSitePlusiP09405.
SwissPalmiP09405.

2D gel databases

REPRODUCTION-2DPAGEP09405.

Proteomic databases

EPDiP09405.
MaxQBiP09405.
PaxDbiP09405.
PeptideAtlasiP09405.
PRIDEiP09405.
TopDownProteomicsiP09405.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027438; ENSMUSP00000027438; ENSMUSG00000026234.
GeneIDi17975.
KEGGimmu:17975.
UCSCiuc007bvl.1. mouse.

Organism-specific databases

CTDi4691.
MGIiMGI:97286. Ncl.

Phylogenomic databases

eggNOGiENOG410IRS7. Eukaryota.
ENOG410XSFV. LUCA.
GeneTreeiENSGT00840000129894.
HOGENOMiHOG000113885.
HOVERGENiHBG002295.
InParanoidiP09405.
KOiK11294.
OMAiCNKMEIE.
OrthoDBiEOG091G15MK.
PhylomeDBiP09405.
TreeFamiTF328499.

Miscellaneous databases

ChiTaRSiNcl. mouse.
PMAP-CutDBP09405.
PROiP09405.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026234.
CleanExiMM_NCL.
ExpressionAtlasiP09405. baseline and differential.
GenevisibleiP09405. MM.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUCL_MOUSE
AccessioniPrimary (citable) accession number: P09405
Secondary accession number(s): Q548M9
, Q61991, Q8BQD8, Q99K50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 175 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.