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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (TTHA0003)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34SubstrateBy similarity1
Binding sitei115SubstrateBy similarity1
Binding sitei148SubstrateBy similarity1
Binding sitei198ATPBy similarity1
Binding sitei289ATP; via carbonyl oxygenBy similarity1
Binding sitei320ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi347 – 350ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:TTHA0906
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001460272 – 390Phosphoglycerate kinaseAdd BLAST389

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi300852.TTHA0906.

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi13 – 17Combined sources5
Beta strandi28 – 30Combined sources3
Helixi33 – 47Combined sources15
Beta strandi51 – 55Combined sources5
Helixi66 – 68Combined sources3
Helixi71 – 80Combined sources10
Beta strandi84 – 86Combined sources3
Helixi94 – 101Combined sources8
Beta strandi108 – 110Combined sources3
Helixi114 – 116Combined sources3
Turni118 – 123Combined sources6
Helixi125 – 131Combined sources7
Helixi132 – 134Combined sources3
Beta strandi136 – 140Combined sources5
Helixi143 – 145Combined sources3
Turni151 – 155Combined sources5
Helixi156 – 158Combined sources3
Beta strandi162 – 164Combined sources3
Helixi166 – 176Combined sources11
Turni177 – 179Combined sources3
Beta strandi184 – 190Combined sources7
Helixi195 – 197Combined sources3
Helixi199 – 205Combined sources7
Helixi206 – 208Combined sources3
Beta strandi210 – 214Combined sources5
Helixi219 – 225Combined sources7
Helixi237 – 239Combined sources3
Helixi240 – 253Combined sources14
Beta strandi256 – 258Combined sources3
Beta strandi261 – 268Combined sources8
Beta strandi276 – 279Combined sources4
Beta strandi288 – 292Combined sources5
Helixi294 – 303Combined sources10
Turni304 – 306Combined sources3
Beta strandi308 – 314Combined sources7
Turni322 – 325Combined sources4
Helixi326 – 336Combined sources11
Beta strandi342 – 347Combined sources6
Helixi348 – 355Combined sources8
Turni356 – 358Combined sources3
Helixi360 – 362Combined sources3
Beta strandi363 – 366Combined sources4
Beta strandi369 – 371Combined sources3
Helixi372 – 379Combined sources8
Helixi383 – 386Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V6SX-ray1.50A/B1-390[»]
ProteinModelPortaliP09403.
SMRiP09403.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09403.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 21Substrate bindingBy similarity3
Regioni57 – 60Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105BZA. Bacteria.
COG0126. LUCA.
HOGENOMiHOG000227108.
KOiK00927.
OMAiAGHPVGK.
PhylomeDBiP09403.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09403-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLLDLDPK GKRVLVRVDY NVPVQDGKVQ DETRILESLP TLRHLLAGGA
60 70 80 90 100
SLVLLSHLGR PKGPDPKYSL APVGEALRAH LPEARFAPFP PGSEEARREA
110 120 130 140 150
EALRPGEVLL LENVRFEPGE EKNDPELSAR YARLGEAFVL DAFGSAHRAH
160 170 180 190 200
ASVVGVARLL PAYAGFLMEK EVRALSRLLK DPERPYAVVL GGAKVSDKIG
210 220 230 240 250
VIESLLPRID RLLIGGAMAF TFLKALGGEV GRSLVEEDRL DLAKDLLGRA
260 270 280 290 300
EALGVRVYLP EDVVAAERIE AGVETRVFPA RAIPVPYMGL DIGPKTREAF
310 320 330 340 350
ARALEGARTV FWNGPMGVFE VPPFDEGTLA VGQAIAALEG AFTVVGGGDS
360 370 380 390
VAAVNRLGLK ERFGHVSTGG GASLEFLEKG TLPGLEVLEG
Length:390
Mass (Da):41,777
Last modified:January 23, 2007 - v3
Checksum:i3829E89FCEDC90B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12464 Genomic DNA. Translation: CAA31006.1.
AP008226 Genomic DNA. Translation: BAD70729.1.
RefSeqiWP_011228285.1. NC_006461.1.
YP_144172.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70729; BAD70729; BAD70729.
GeneIDi3169950.
KEGGittj:TTHA0906.
PATRICi23956792. VBITheThe93045_0898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12464 Genomic DNA. Translation: CAA31006.1.
AP008226 Genomic DNA. Translation: BAD70729.1.
RefSeqiWP_011228285.1. NC_006461.1.
YP_144172.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V6SX-ray1.50A/B1-390[»]
ProteinModelPortaliP09403.
SMRiP09403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0906.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70729; BAD70729; BAD70729.
GeneIDi3169950.
KEGGittj:TTHA0906.
PATRICi23956792. VBITheThe93045_0898.

Phylogenomic databases

eggNOGiENOG4105BZA. Bacteria.
COG0126. LUCA.
HOGENOMiHOG000227108.
KOiK00927.
OMAiAGHPVGK.
PhylomeDBiP09403.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Miscellaneous databases

EvolutionaryTraceiP09403.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK_THET8
AccessioniPrimary (citable) accession number: P09403
Secondary accession number(s): Q5SJV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.