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P09403 (PGK_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase
EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:TTHA0906
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus ›

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed HAMAP-Rule MF_00145
Chain2 – 390389Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000146027

Regions

Nucleotide binding347 – 3504ATP By similarity
Region19 – 213Substrate binding By similarity
Region57 – 604Substrate binding By similarity

Sites

Binding site341Substrate By similarity
Binding site1151Substrate By similarity
Binding site1481Substrate By similarity
Binding site1981ATP By similarity
Binding site2891ATP; via carbonyl oxygen By similarity
Binding site3201ATP By similarity

Secondary structure

1 .................................................................................. 390
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P09403 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3829E89FCEDC90B3

FASTA39041,777
        10         20         30         40         50         60 
MRTLLDLDPK GKRVLVRVDY NVPVQDGKVQ DETRILESLP TLRHLLAGGA SLVLLSHLGR 

        70         80         90        100        110        120 
PKGPDPKYSL APVGEALRAH LPEARFAPFP PGSEEARREA EALRPGEVLL LENVRFEPGE 

       130        140        150        160        170        180 
EKNDPELSAR YARLGEAFVL DAFGSAHRAH ASVVGVARLL PAYAGFLMEK EVRALSRLLK 

       190        200        210        220        230        240 
DPERPYAVVL GGAKVSDKIG VIESLLPRID RLLIGGAMAF TFLKALGGEV GRSLVEEDRL 

       250        260        270        280        290        300 
DLAKDLLGRA EALGVRVYLP EDVVAAERIE AGVETRVFPA RAIPVPYMGL DIGPKTREAF 

       310        320        330        340        350        360 
ARALEGARTV FWNGPMGVFE VPPFDEGTLA VGQAIAALEG AFTVVGGGDS VAAVNRLGLK 

       370        380        390 
ERFGHVSTGG GASLEFLEKG TLPGLEVLEG

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the phosphoglycerate kinase gene from the extreme thermophile Thermus thermophilus. Comparison of the deduced amino acid sequence with that of the mesophilic yeast phosphoglycerate kinase."
Bowen D., Littlechild J.A., Fothergill J.E., Watson H.C., Hall L.
Biochem. J. 254:509-517(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X12464 Genomic DNA. Translation: CAA31006.1.
AP008226 Genomic DNA. Translation: BAD70729.1.
RefSeqYP_144172.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1V6SX-ray1.50A/B1-390[»]
ProteinModelPortalP09403.
SMRP09403. Positions 1-390.
ModBaseSearch...

Protein-protein interaction databases

STRING300852.TTHA0906.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD70729; BAD70729; BAD70729.
GeneID3169950.
KEGGttj:TTHA0906.
PATRIC23956792. VBITheThe93045_0898.

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227108.
KOK00927.
OMAYADVIHI.
ProtClustDBPRK00073.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-939-MONOMER.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. PGK. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP09403.

Entry information

Entry namePGK_THET8
AccessionPrimary (citable) accession number: P09403
Secondary accession number(s): Q5SJV2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: May 29, 2013
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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