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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341SubstrateBy similarity
Binding sitei115 – 1151SubstrateBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Binding sitei198 – 1981ATPBy similarity
Binding sitei289 – 2891ATP; via carbonyl oxygenBy similarity
Binding sitei320 – 3201ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi347 – 3504ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoglycerate kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-939-MONOMER.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:TTHA0906
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 390389Phosphoglycerate kinasePRO_0000146027Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi300852.TTHA0906.

Structurei

Secondary structure

1
390
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Beta strandi13 – 175Combined sources
Beta strandi28 – 303Combined sources
Helixi33 – 4715Combined sources
Beta strandi51 – 555Combined sources
Helixi66 – 683Combined sources
Helixi71 – 8010Combined sources
Beta strandi84 – 863Combined sources
Helixi94 – 1018Combined sources
Beta strandi108 – 1103Combined sources
Helixi114 – 1163Combined sources
Turni118 – 1236Combined sources
Helixi125 – 1317Combined sources
Helixi132 – 1343Combined sources
Beta strandi136 – 1405Combined sources
Helixi143 – 1453Combined sources
Turni151 – 1555Combined sources
Helixi156 – 1583Combined sources
Beta strandi162 – 1643Combined sources
Helixi166 – 17611Combined sources
Turni177 – 1793Combined sources
Beta strandi184 – 1907Combined sources
Helixi195 – 1973Combined sources
Helixi199 – 2057Combined sources
Helixi206 – 2083Combined sources
Beta strandi210 – 2145Combined sources
Helixi219 – 2257Combined sources
Helixi237 – 2393Combined sources
Helixi240 – 25314Combined sources
Beta strandi256 – 2583Combined sources
Beta strandi261 – 2688Combined sources
Beta strandi276 – 2794Combined sources
Beta strandi288 – 2925Combined sources
Helixi294 – 30310Combined sources
Turni304 – 3063Combined sources
Beta strandi308 – 3147Combined sources
Turni322 – 3254Combined sources
Helixi326 – 33611Combined sources
Beta strandi342 – 3476Combined sources
Helixi348 – 3558Combined sources
Turni356 – 3583Combined sources
Helixi360 – 3623Combined sources
Beta strandi363 – 3664Combined sources
Beta strandi369 – 3713Combined sources
Helixi372 – 3798Combined sources
Helixi383 – 3864Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V6SX-ray1.50A/B1-390[»]
ProteinModelPortaliP09403.
SMRiP09403. Positions 1-390.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09403.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 213Substrate bindingBy similarity
Regioni57 – 604Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227108.
KOiK00927.
OMAiDAFGTCH.
OrthoDBiEOG64N9Z0.
PhylomeDBiP09403.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09403-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLLDLDPK GKRVLVRVDY NVPVQDGKVQ DETRILESLP TLRHLLAGGA
60 70 80 90 100
SLVLLSHLGR PKGPDPKYSL APVGEALRAH LPEARFAPFP PGSEEARREA
110 120 130 140 150
EALRPGEVLL LENVRFEPGE EKNDPELSAR YARLGEAFVL DAFGSAHRAH
160 170 180 190 200
ASVVGVARLL PAYAGFLMEK EVRALSRLLK DPERPYAVVL GGAKVSDKIG
210 220 230 240 250
VIESLLPRID RLLIGGAMAF TFLKALGGEV GRSLVEEDRL DLAKDLLGRA
260 270 280 290 300
EALGVRVYLP EDVVAAERIE AGVETRVFPA RAIPVPYMGL DIGPKTREAF
310 320 330 340 350
ARALEGARTV FWNGPMGVFE VPPFDEGTLA VGQAIAALEG AFTVVGGGDS
360 370 380 390
VAAVNRLGLK ERFGHVSTGG GASLEFLEKG TLPGLEVLEG
Length:390
Mass (Da):41,777
Last modified:January 22, 2007 - v3
Checksum:i3829E89FCEDC90B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12464 Genomic DNA. Translation: CAA31006.1.
AP008226 Genomic DNA. Translation: BAD70729.1.
RefSeqiWP_011228285.1. NC_006461.1.
YP_144172.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70729; BAD70729; BAD70729.
GeneIDi3169950.
KEGGittj:TTHA0906.
PATRICi23956792. VBITheThe93045_0898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12464 Genomic DNA. Translation: CAA31006.1.
AP008226 Genomic DNA. Translation: BAD70729.1.
RefSeqiWP_011228285.1. NC_006461.1.
YP_144172.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V6SX-ray1.50A/B1-390[»]
ProteinModelPortaliP09403.
SMRiP09403. Positions 1-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0906.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70729; BAD70729; BAD70729.
GeneIDi3169950.
KEGGittj:TTHA0906.
PATRICi23956792. VBITheThe93045_0898.

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227108.
KOiK00927.
OMAiDAFGTCH.
OrthoDBiEOG64N9Z0.
PhylomeDBiP09403.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciTTHE300852:GH8R-939-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP09403.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the phosphoglycerate kinase gene from the extreme thermophile Thermus thermophilus. Comparison of the deduced amino acid sequence with that of the mesophilic yeast phosphoglycerate kinase."
    Bowen D., Littlechild J.A., Fothergill J.E., Watson H.C., Hall L.
    Biochem. J. 254:509-517(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiPGK_THET8
AccessioniPrimary (citable) accession number: P09403
Secondary accession number(s): Q5SJV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1989
Last sequence update: January 22, 2007
Last modified: January 6, 2015
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.