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Protein

Streptomycin-6-phosphate phosphatase

Gene

strK

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin.

Catalytic activityi

Streptomycin 6-phosphate + H2O = streptomycin + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Pathwayi: streptomycin biosynthesis

This protein is involved in the pathway streptomycin biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway streptomycin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50MagnesiumBy similarity1
Metal bindingi50Zinc 2By similarity1
Active sitei99Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi151MagnesiumBy similarity1
Metal bindingi153MagnesiumBy similarity1
Metal bindingi321MagnesiumBy similarity1
Metal bindingi326Zinc 1By similarity1
Metal bindingi330Zinc 1By similarity1
Metal bindingi368Zinc 2By similarity1
Metal bindingi369Zinc 2By similarity1
Metal bindingi412Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAntibiotic biosynthesis, Streptomycin biosynthesis
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14015.
UniPathwayiUPA00066.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptomycin-6-phosphate phosphatase (EC:3.1.3.39)
Gene namesi
Name:strK
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000002401633 – 449Streptomycin-6-phosphate phosphataseAdd BLAST417

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi455632.SGR_5938.

Structurei

3D structure databases

ProteinModelPortaliP09401.
SMRiP09401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
PANTHERiPTHR11596. PTHR11596. 1 hit.
PfamiView protein in Pfam
PF00245. Alk_phosphatase. 2 hits.
PRINTSiPR00113. ALKPHPHTASE.
SMARTiView protein in SMART
SM00098. alkPPc. 1 hit.
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiView protein in PROSITE
PS00123. ALKALINE_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFAYGRLPW RRGAVLGSAL LVLVTAPAAS TATERSGPAP ARSVILLIGD
60 70 80 90 100
GMGDAEITAA RNYSVGAAGR LAMDTLDASG RRTTYAVDER GRPVYVTDSA
110 120 130 140 150
AGATAWATGR RTVNGRVSKS HDTDRPMPTL LELARDRGYA TGSVTTASVA
160 170 180 190 200
DATPAALTAH VTDRSCKGPA DMAACPADTR AGGGEGSIAE QTVAARPDVL
210 220 230 240 250
LGGGADHFAQ TVTDGPFRGR TVTQQARAAG YQVVRDRTQL AAARPGRPVL
260 270 280 290 300
GLFAPEYVPV EWTGPPAAPG GTAPQRCATR NPGRPAGTPD LAESTRAALD
310 320 330 340 350
LLTARAHHRG APGRGFFLQV EGASIDDRAH EADPCGQLGE TLAFDRAVAA
360 370 380 390 400
ALDHAERHPR TLVIVTADHG HATQILPHDA RPAGLSATLV TDEGGVMQLG
410 420 430 440
YGTALPGETQ EHTGVPVPVA ARGPLADRVR GVQDNTSLFG TVTAALGLR
Length:449
Mass (Da):46,343
Last modified:August 1, 1992 - v2
Checksum:i2B7BC9393F9686E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00459 Genomic DNA. Translation: CAA68522.1.
PIRiS17780.
RefSeqiWP_003970245.1. NZ_MDDK01000534.1.

Genome annotation databases

GeneIDi6209972.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00459 Genomic DNA. Translation: CAA68522.1.
PIRiS17780.
RefSeqiWP_003970245.1. NZ_MDDK01000534.1.

3D structure databases

ProteinModelPortaliP09401.
SMRiP09401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi455632.SGR_5938.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi6209972.

Phylogenomic databases

eggNOGiENOG4105G6Z. Bacteria.
COG1785. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00066.
BioCyciMetaCyc:MONOMER-14015.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
PANTHERiPTHR11596. PTHR11596. 1 hit.
PfamiView protein in Pfam
PF00245. Alk_phosphatase. 2 hits.
PRINTSiPR00113. ALKPHPHTASE.
SMARTiView protein in SMART
SM00098. alkPPc. 1 hit.
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiView protein in PROSITE
PS00123. ALKALINE_PHOSPHATASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTRK_STRGR
AccessioniPrimary (citable) accession number: P09401
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 1, 1992
Last modified: March 15, 2017
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.