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Protein

Streptomycin-6-phosphate phosphatase

Gene

strK

Organism
Streptomyces griseus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin.

Catalytic activityi

Streptomycin 6-phosphate + H2O = streptomycin + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501MagnesiumBy similarity
Metal bindingi50 – 501Zinc 2By similarity
Active sitei99 – 991Phosphoserine intermediatePROSITE-ProRule annotation
Metal bindingi151 – 1511MagnesiumBy similarity
Metal bindingi153 – 1531MagnesiumBy similarity
Metal bindingi321 – 3211MagnesiumBy similarity
Metal bindingi326 – 3261Zinc 1By similarity
Metal bindingi330 – 3301Zinc 1By similarity
Metal bindingi368 – 3681Zinc 2By similarity
Metal bindingi369 – 3691Zinc 2By similarity
Metal bindingi412 – 4121Zinc 1By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. streptomycin-6-phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. streptomycin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic biosynthesis, Streptomycin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14015.
UniPathwayiUPA00066.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptomycin-6-phosphate phosphatase (EC:3.1.3.39)
Gene namesi
Name:strK
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 449417Streptomycin-6-phosphate phosphatasePRO_0000024016Add
BLAST

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP09401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFAYGRLPW RRGAVLGSAL LVLVTAPAAS TATERSGPAP ARSVILLIGD
60 70 80 90 100
GMGDAEITAA RNYSVGAAGR LAMDTLDASG RRTTYAVDER GRPVYVTDSA
110 120 130 140 150
AGATAWATGR RTVNGRVSKS HDTDRPMPTL LELARDRGYA TGSVTTASVA
160 170 180 190 200
DATPAALTAH VTDRSCKGPA DMAACPADTR AGGGEGSIAE QTVAARPDVL
210 220 230 240 250
LGGGADHFAQ TVTDGPFRGR TVTQQARAAG YQVVRDRTQL AAARPGRPVL
260 270 280 290 300
GLFAPEYVPV EWTGPPAAPG GTAPQRCATR NPGRPAGTPD LAESTRAALD
310 320 330 340 350
LLTARAHHRG APGRGFFLQV EGASIDDRAH EADPCGQLGE TLAFDRAVAA
360 370 380 390 400
ALDHAERHPR TLVIVTADHG HATQILPHDA RPAGLSATLV TDEGGVMQLG
410 420 430 440
YGTALPGETQ EHTGVPVPVA ARGPLADRVR GVQDNTSLFG TVTAALGLR
Length:449
Mass (Da):46,343
Last modified:August 1, 1992 - v2
Checksum:i2B7BC9393F9686E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00459 Genomic DNA. Translation: CAA68522.1.
PIRiS17780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00459 Genomic DNA. Translation: CAA68522.1.
PIRiS17780.

3D structure databases

ProteinModelPortaliP09401.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00066.
BioCyciMetaCyc:MONOMER-14015.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genetics of streptomycin production in Streptomyces griseus: nucleotide sequence of five genes, strFGHIK, including a phosphatase gene."
    Mansouri K., Piepersberg W.
    Mol. Gen. Genet. 228:459-469(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: N2-3-11.

Entry informationi

Entry nameiSTRK_STRGR
AccessioniPrimary (citable) accession number: P09401
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 1, 1992
Last modified: January 7, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.