Reviewed, UniProtKB/Swiss-Prot P09401 (STRK_STRGR)

Names and origin

Protein names

Recommended name:
    Streptomycin-6-phosphate phosphatase
    EC=3.1.3.39

Gene names

Name:

strK

Organism

Streptomyces griseus

Taxonomic identifier

1911 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin.
Catalytic activity	Streptomycin 6-phosphate + H2O = streptomycin + phosphate.
Cofactor	Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity.
Pathway	Antibiotic biosynthesis; streptomycin biosynthesis.
Subcellular location	Secreted.
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis Streptomycin biosynthesis
Cellular component	Secreted
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	streptomycin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW streptomycin-6-phosphatase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 449	417	Streptomycin-6-phosphate phosphatase		PRO_0000024016
Sites
	Active site	99	1	Phosphoserine intermediate By similarity
	Metal binding	50	1	Magnesium By similarity
	Metal binding	50	1	Zinc 2 By similarity
	Metal binding	151	1	Magnesium By similarity
	Metal binding	153	1	Magnesium By similarity
	Metal binding	321	1	Magnesium By similarity
	Metal binding	326	1	Zinc 1 By similarity
	Metal binding	330	1	Zinc 1 By similarity
	Metal binding	368	1	Zinc 2 By similarity
	Metal binding	369	1	Zinc 2 By similarity
	Metal binding	412	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09401-1 [UniParc]. Last modified August 1, 1992. Version 2. Checksum: 2B7BC9393F9686E8	FASTA	449	46,343
	10 20 30 40 50 60 MRFAYGRLPW RRGAVLGSAL LVLVTAPAAS TATERSGPAP ARSVILLIGD GMGDAEITAA 70 80 90 100 110 120 RNYSVGAAGR LAMDTLDASG RRTTYAVDER GRPVYVTDSA AGATAWATGR RTVNGRVSKS 130 140 150 160 170 180 HDTDRPMPTL LELARDRGYA TGSVTTASVA DATPAALTAH VTDRSCKGPA DMAACPADTR 190 200 210 220 230 240 AGGGEGSIAE QTVAARPDVL LGGGADHFAQ TVTDGPFRGR TVTQQARAAG YQVVRDRTQL 250 260 270 280 290 300 AAARPGRPVL GLFAPEYVPV EWTGPPAAPG GTAPQRCATR NPGRPAGTPD LAESTRAALD 310 320 330 340 350 360 LLTARAHHRG APGRGFFLQV EGASIDDRAH EADPCGQLGE TLAFDRAVAA ALDHAERHPR 370 380 390 400 410 420 TLVIVTADHG HATQILPHDA RPAGLSATLV TDEGGVMQLG YGTALPGETQ EHTGVPVPVA 430 440 ARGPLADRVR GVQDNTSLFG TVTAALGLR

References

[1]	"Genetics of streptomycin production in Streptomyces griseus: nucleotide sequence of five genes, strFGHIK, including a phosphatase gene." Mansouri K., Piepersberg W. Mol. Gen. Genet. 228:459-469(1991) [PubMed: 1654502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: N2-3-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00459 Genomic DNA. Translation: CAA68522.1.
PIR	S17780.
3D structure databases
HSSP	HSSP built from PDB template 1AJA based on UniProtKB P00634.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-14015.
BRENDA	3.1.3.39. 1270.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STRK_STRGR

Accession

Primary (citable) accession number: P09401

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)