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P09401 (STRK_STRGR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Streptomycin-6-phosphate phosphatase

EC=3.1.3.39
Gene names
Name:strK
OrganismStreptomyces griseus
Taxonomic identifier1911 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin.

Catalytic activity

Streptomycin 6-phosphate + H2O = streptomycin + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Pathway

Antibiotic biosynthesis; streptomycin biosynthesis.

Subcellular location

Secreted.

Sequence similarities

Belongs to the alkaline phosphatase family.

Ontologies

Keywords
   Biological processAntibiotic biosynthesis
Streptomycin biosynthesis
   Cellular componentSecreted
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processstreptomycin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

streptomycin-6-phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 449417Streptomycin-6-phosphate phosphatase
PRO_0000024016

Sites

Active site991Phosphoserine intermediate By similarity
Metal binding501Magnesium By similarity
Metal binding501Zinc 2 By similarity
Metal binding1511Magnesium By similarity
Metal binding1531Magnesium By similarity
Metal binding3211Magnesium By similarity
Metal binding3261Zinc 1 By similarity
Metal binding3301Zinc 1 By similarity
Metal binding3681Zinc 2 By similarity
Metal binding3691Zinc 2 By similarity
Metal binding4121Zinc 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P09401 [UniParc].

Last modified August 1, 1992. Version 2.
Checksum: 2B7BC9393F9686E8

FASTA44946,343
        10         20         30         40         50         60 
MRFAYGRLPW RRGAVLGSAL LVLVTAPAAS TATERSGPAP ARSVILLIGD GMGDAEITAA 

        70         80         90        100        110        120 
RNYSVGAAGR LAMDTLDASG RRTTYAVDER GRPVYVTDSA AGATAWATGR RTVNGRVSKS 

       130        140        150        160        170        180 
HDTDRPMPTL LELARDRGYA TGSVTTASVA DATPAALTAH VTDRSCKGPA DMAACPADTR 

       190        200        210        220        230        240 
AGGGEGSIAE QTVAARPDVL LGGGADHFAQ TVTDGPFRGR TVTQQARAAG YQVVRDRTQL 

       250        260        270        280        290        300 
AAARPGRPVL GLFAPEYVPV EWTGPPAAPG GTAPQRCATR NPGRPAGTPD LAESTRAALD 

       310        320        330        340        350        360 
LLTARAHHRG APGRGFFLQV EGASIDDRAH EADPCGQLGE TLAFDRAVAA ALDHAERHPR 

       370        380        390        400        410        420 
TLVIVTADHG HATQILPHDA RPAGLSATLV TDEGGVMQLG YGTALPGETQ EHTGVPVPVA 

       430        440 
ARGPLADRVR GVQDNTSLFG TVTAALGLR 

« Hide

References

[1]"Genetics of streptomycin production in Streptomyces griseus: nucleotide sequence of five genes, strFGHIK, including a phosphatase gene."
Mansouri K., Piepersberg W.
Mol. Gen. Genet. 228:459-469(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: N2-3-11.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00459 Genomic DNA. Translation: CAA68522.1.
PIRS17780.

3D structure databases

ProteinModelPortalP09401.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14015.
UniPathwayUPA00066.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSTRK_STRGR
AccessionPrimary (citable) accession number: P09401
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways