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P09401

- STRK_STRGR

UniProt

P09401 - STRK_STRGR

Protein

Streptomycin-6-phosphate phosphatase

Gene

strK

Organism
Streptomyces griseus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin.

    Catalytic activityi

    Streptomycin 6-phosphate + H2O = streptomycin + phosphate.

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi50 – 501MagnesiumBy similarity
    Metal bindingi50 – 501Zinc 2By similarity
    Active sitei99 – 991Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi151 – 1511MagnesiumBy similarity
    Metal bindingi153 – 1531MagnesiumBy similarity
    Metal bindingi321 – 3211MagnesiumBy similarity
    Metal bindingi326 – 3261Zinc 1By similarity
    Metal bindingi330 – 3301Zinc 1By similarity
    Metal bindingi368 – 3681Zinc 2By similarity
    Metal bindingi369 – 3691Zinc 2By similarity
    Metal bindingi412 – 4121Zinc 1By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. streptomycin-6-phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. streptomycin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic biosynthesis, Streptomycin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14015.
    UniPathwayiUPA00066.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Streptomycin-6-phosphate phosphatase (EC:3.1.3.39)
    Gene namesi
    Name:strK
    OrganismiStreptomyces griseus
    Taxonomic identifieri1911 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 449417Streptomycin-6-phosphate phosphatasePRO_0000024016Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliP09401.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 2 hits.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09401-1 [UniParc]FASTAAdd to Basket

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    MRFAYGRLPW RRGAVLGSAL LVLVTAPAAS TATERSGPAP ARSVILLIGD    50
    GMGDAEITAA RNYSVGAAGR LAMDTLDASG RRTTYAVDER GRPVYVTDSA 100
    AGATAWATGR RTVNGRVSKS HDTDRPMPTL LELARDRGYA TGSVTTASVA 150
    DATPAALTAH VTDRSCKGPA DMAACPADTR AGGGEGSIAE QTVAARPDVL 200
    LGGGADHFAQ TVTDGPFRGR TVTQQARAAG YQVVRDRTQL AAARPGRPVL 250
    GLFAPEYVPV EWTGPPAAPG GTAPQRCATR NPGRPAGTPD LAESTRAALD 300
    LLTARAHHRG APGRGFFLQV EGASIDDRAH EADPCGQLGE TLAFDRAVAA 350
    ALDHAERHPR TLVIVTADHG HATQILPHDA RPAGLSATLV TDEGGVMQLG 400
    YGTALPGETQ EHTGVPVPVA ARGPLADRVR GVQDNTSLFG TVTAALGLR 449
    Length:449
    Mass (Da):46,343
    Last modified:August 1, 1992 - v2
    Checksum:i2B7BC9393F9686E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00459 Genomic DNA. Translation: CAA68522.1.
    PIRiS17780.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00459 Genomic DNA. Translation: CAA68522.1 .
    PIRi S17780.

    3D structure databases

    ProteinModelPortali P09401.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00066 .
    BioCyci MetaCyc:MONOMER-14015.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 2 hits.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetics of streptomycin production in Streptomyces griseus: nucleotide sequence of five genes, strFGHIK, including a phosphatase gene."
      Mansouri K., Piepersberg W.
      Mol. Gen. Genet. 228:459-469(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: N2-3-11.

    Entry informationi

    Entry nameiSTRK_STRGR
    AccessioniPrimary (citable) accession number: P09401
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3