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P09401

- STRK_STRGR

UniProt

P09401 - STRK_STRGR

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Protein

Streptomycin-6-phosphate phosphatase

Gene
strK
Organism
Streptomyces griseus
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Specifically cleaves both streptomycin-6-phosphate and, more slowly, streptomycin-3''-phosphate during the biosynthesis of streptomycin.

Catalytic activityi

Streptomycin 6-phosphate + H2O = streptomycin + phosphate.

Cofactori

Binds 1 magnesium ion By similarity.
Binds 2 zinc ions By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Magnesium By similarity
Metal bindingi50 – 501Zinc 2 By similarity
Active sitei99 – 991Phosphoserine intermediate By similarity
Metal bindingi151 – 1511Magnesium By similarity
Metal bindingi153 – 1531Magnesium By similarity
Metal bindingi321 – 3211Magnesium By similarity
Metal bindingi326 – 3261Zinc 1 By similarity
Metal bindingi330 – 3301Zinc 1 By similarity
Metal bindingi368 – 3681Zinc 2 By similarity
Metal bindingi369 – 3691Zinc 2 By similarity
Metal bindingi412 – 4121Zinc 1 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. streptomycin-6-phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. streptomycin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic biosynthesis, Streptomycin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14015.
UniPathwayiUPA00066.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptomycin-6-phosphate phosphatase (EC:3.1.3.39)
Gene namesi
Name:strK
OrganismiStreptomyces griseus
Taxonomic identifieri1911 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 Reviewed predictionAdd
BLAST
Chaini33 – 449417Streptomycin-6-phosphate phosphatasePRO_0000024016Add
BLAST

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP09401.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09401-1 [UniParc]FASTAAdd to Basket

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MRFAYGRLPW RRGAVLGSAL LVLVTAPAAS TATERSGPAP ARSVILLIGD    50
GMGDAEITAA RNYSVGAAGR LAMDTLDASG RRTTYAVDER GRPVYVTDSA 100
AGATAWATGR RTVNGRVSKS HDTDRPMPTL LELARDRGYA TGSVTTASVA 150
DATPAALTAH VTDRSCKGPA DMAACPADTR AGGGEGSIAE QTVAARPDVL 200
LGGGADHFAQ TVTDGPFRGR TVTQQARAAG YQVVRDRTQL AAARPGRPVL 250
GLFAPEYVPV EWTGPPAAPG GTAPQRCATR NPGRPAGTPD LAESTRAALD 300
LLTARAHHRG APGRGFFLQV EGASIDDRAH EADPCGQLGE TLAFDRAVAA 350
ALDHAERHPR TLVIVTADHG HATQILPHDA RPAGLSATLV TDEGGVMQLG 400
YGTALPGETQ EHTGVPVPVA ARGPLADRVR GVQDNTSLFG TVTAALGLR 449
Length:449
Mass (Da):46,343
Last modified:August 1, 1992 - v2
Checksum:i2B7BC9393F9686E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00459 Genomic DNA. Translation: CAA68522.1.
PIRiS17780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00459 Genomic DNA. Translation: CAA68522.1 .
PIRi S17780.

3D structure databases

ProteinModelPortali P09401.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00066 .
BioCyci MetaCyc:MONOMER-14015.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetics of streptomycin production in Streptomyces griseus: nucleotide sequence of five genes, strFGHIK, including a phosphatase gene."
    Mansouri K., Piepersberg W.
    Mol. Gen. Genet. 228:459-469(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: N2-3-11.

Entry informationi

Entry nameiSTRK_STRGR
AccessioniPrimary (citable) accession number: P09401
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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