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Protein

RNA replication protein

Gene
N/A
Organism
Potato virus X (PVX)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RNA replication. The central part of this protein possibly functions as an ATP-binding helicase (Probable).Curated

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi735 – 742ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA replication protein
Alternative name(s):
165 kDa protein
ORF1 protein
Including the following 2 domains:
RNA-directed RNA polymerase (EC:2.7.7.48)
Helicase (EC:3.6.4.13)
OrganismiPotato virus X (PVX)
Taxonomic identifieri12183 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageTymoviralesAlphaflexiviridaePotexvirus
Virus hostiBrassica campestris (Field mustard) [TaxID: 3711]
Solanum tuberosum (Potato) [TaxID: 4113]
Proteomesi
  • UP000006841 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002225531 – 1456RNA replication proteinAdd BLAST1456

Structurei

3D structure databases

ProteinModelPortaliP09395.
SMRiP09395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 224Alphavirus-like MTPROSITE-ProRule annotationAdd BLAST166
Domaini695 – 862(+)RNA virus helicase ATP-bindingAdd BLAST168
Domaini863 – 997(+)RNA virus helicase C-terminalAdd BLAST135
Domaini1236 – 1343RdRp catalyticPROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG0900004V.

Family and domain databases

InterProiView protein in InterPro
IPR027351. (+)RNA_virus_helicase_core_dom.
IPR002588. Alphavirus-like_MT_dom.
IPR027417. P-loop_NTPase.
IPR007094. RNA-dir_pol_PSvirus.
IPR001788. Tymovirus_RNA-dep_RNA_pol.
PfamiView protein in Pfam
PF00978. RdRP_2. 1 hit.
PF01443. Viral_helicase1. 1 hit.
PF01660. Vmethyltransf. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS51743. ALPHAVIRUS_MT. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

P09395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVREVYQS FTDSTTKTLI QDEAYRNIRP IMEKHKLANP YAQTVEAAND
60 70 80 90 100
LEGFGIATNP YSIELHTHAA AKTIENKLLE VLGSILPQEP VTFMFLKPRK
110 120 130 140 150
LNYMRRNPRI KDIFHNVAIE PRDVARYPKE TIIDKLTEIT TDTAYIGDTL
160 170 180 190 200
HFLDPSYIVE TFQNCPKLQT LYATLVLPVE AAFKMESTHP NIYSLKYFGD
210 220 230 240 250
GFQYIPGNHG GGAYHHEFSH LQWLKVGKIK WRDPKDSFLG HLNYTTEQVE
260 270 280 290 300
MHTVTVQLQE SFAANHLYCI RRGDLLTPEV RTFGQPDRYV IPPQIFLPKV
310 320 330 340 350
HNCKKPILKK TMMQLFLYVR TVKVAKNCDI FAKVRQLIKS SDLDKYSAVE
360 370 380 390 400
LVYLVSYMEF LADLQATTCF SDTLSGGLLT KTLAPVRAWI QEKKMQLFGL
410 420 430 440 450
EDYAKLVKAV DFHPVDFSFK VETWDFRFHP LQAWKAFRPR EVSDVEEMEN
460 470 480 490 500
LFSDGDLLDC FTRMPAYAVN AEEDLAAIRK TPEMDAGQEV KEPAGDRNQY
510 520 530 540 550
SNPAETFLSK LHRKHSREVK HQAAKKAKRL AEIQESMRAE GEAESNEMSG
560 570 580 590 600
GMGAIPSNAE LPSTSGARQE LTLPTTKPVP ARWEDASFTD SSVEEEQVKL
610 620 630 640 650
PGKEAVETAT QQVIEGLPWK HWIPQLNAVG FKALEIQRDR SGTMIMPITE
660 670 680 690 700
MVSGLEKEDF PEGTPKELAR ELLAMNRSPA TIPLDLLRAR DYGSDVKNKR
710 720 730 740 750
IGAITKTQAA SWGEYLTGKI ESLTERKVAA CVIHGAGGSG KSHAIQKALR
760 770 780 790 800
EIGKGSDITV VLPTNELRLD WSKKVPNTEP YMFKTYEKAL IGGTGSIVIF
810 820 830 840 850
DDYSKLPPGY IEALICFYSK IKLVILTGDS RQSVYHETAE DASIRHLGPA
860 870 880 890 900
TEYFSKYCRY YLNATHRNKK DLANMLGVYS ERTGVTEISM SAEFLEGIPT
910 920 930 940 950
LVPSDEKRRL YMGTGRNDTF TYAGCQGLTK PKVQIVLDHN TQVCSANVMY
960 970 980 990 1000
TALSRATDRI HFVNTSANSS AFWEKLDSTP YLKTFLSVVR EQALKEYEPA
1010 1020 1030 1040 1050
EAEPIREPEP QTHMCVENEE SVLEEYKEEL LEKFDREIHS ESHGHSNCVQ
1060 1070 1080 1090 1100
TEDTTIQLFS HQQAKDETLL WATIDARLKT SNQETNFREF LSKKDIGDVL
1110 1120 1130 1140 1150
FLNYQKAMGL PKERIPFSQE VWEACAHEVQ SKYLSKSKCN LINGTVRQSP
1160 1170 1180 1190 1200
DFDENKIMVF LKSQWVTKVE KLGLPKIKPG QTIAAFYQQT VMLFGTMARY
1210 1220 1230 1240 1250
MRWFRQAFQP KEVFINCETT PEDMSAWALN NWNFSRPSLA NDYTAFDQSQ
1260 1270 1280 1290 1300
DGAMLQFEVL KAKHHCIPEE IIQAYIDIKT NAHIFLGTLS IMRLTGEGPT
1310 1320 1330 1340 1350
FDANTECNIA YTHTKFDIPA GTAQVYAGDD SALDCVPEVK HSFHRLEDKL
1360 1370 1380 1390 1400
LLKSKPVITQ QKKGSWPEFC GWLITPKGVM KDPIKLHVSL KLAEAKGELK
1410 1420 1430 1440 1450
KCQDSYEIDL SYAYDHKDSL HDLFDEKQCQ AHTLTCRTLI KSGRGTVSLP

RLRNFL
Length:1,456
Mass (Da):165,407
Last modified:July 1, 1989 - v1
Checksum:iF09FDF47E298988C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M72416 Genomic RNA. Translation: AAA47167.1.

Similar proteinsi

Entry informationi

Entry nameiRDRP_PVX
AccessioniPrimary (citable) accession number: P09395
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 10, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families