Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Rhomboid protease GlpG

Gene

glpG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Rhomboid-type serine protease that catalyzes intramembrane proteolysis.2 Publications

Caution

Was originally identified as a repressor of the glycerol-3-phosphate regulon.1 Publication

Catalytic activityi

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei201Nucleophile4 Publications1
Active sitei2544 Publications1

GO - Molecular functioni

  • endopeptidase activity Source: EcoCyc
  • identical protein binding Source: IntAct
  • serine-type endopeptidase activity Source: EcoCyc

GO - Biological processi

  • proteolysis Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10397-MONOMER
MetaCyc:EG10397-MONOMER
BRENDAi3.4.21.105 2026

Protein family/group databases

MEROPSiS54.016
TCDBi9.B.104.1.1 the rhomboid protease (rhomboid) family

Names & Taxonomyi

Protein namesi
Recommended name:
Rhomboid protease GlpG (EC:3.4.21.105)
Alternative name(s):
Intramembrane serine protease
Gene namesi
Name:glpG
Ordered Locus Names:b3424, JW5687
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10397 glpG

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 93CytoplasmicCuratedAdd BLAST93
Transmembranei94 – 114Helical; Name=1CuratedAdd BLAST21
Topological domaini115 – 141PeriplasmicCuratedAdd BLAST27
Transmembranei142 – 162Helical; Name=2CuratedAdd BLAST21
Topological domaini163 – 168CytoplasmicCurated6
Transmembranei169 – 189Helical; Name=3CuratedAdd BLAST21
Topological domaini190 – 191PeriplasmicCurated2
Transmembranei192 – 212Helical; Name=4CuratedAdd BLAST21
Topological domaini213 – 222CytoplasmicCurated10
Transmembranei223 – 245Helical; Name=5CuratedAdd BLAST23
Topological domaini246 – 249PeriplasmicCurated4
Transmembranei250 – 272Helical; Name=6CuratedAdd BLAST23
Topological domaini273 – 276CytoplasmicCurated4

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi154N → A: Reduced catalytic activity. 2 Publications1
Mutagenesisi199G → C: Loss of catalytic activity. 1 Publication1
Mutagenesisi201S → A or C: Loss of catalytic activity. 4 Publications1
Mutagenesisi254H → A or C: Loss of catalytic activity. 3 Publications1

Chemistry databases

DrugBankiDB04147 Lauryl Dimethylamine-N-Oxide

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000875151 – 276Rhomboid protease GlpGAdd BLAST276

Proteomic databases

PaxDbiP09391
PRIDEiP09391

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-9134140,EBI-9134140

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262105, 40 interactors
DIPiDIP-9796N
IntActiP09391, 3 interactors
MINTiP09391
STRINGi316385.ECDH10B_3598

Chemistry databases

BindingDBiP09391

Structurei

Secondary structure

1276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Helixi10 – 22Combined sources13
Beta strandi27 – 30Combined sources4
Beta strandi32 – 40Combined sources9
Helixi42 – 44Combined sources3
Helixi45 – 57Combined sources13
Helixi62 – 65Combined sources4
Helixi95 – 114Combined sources20
Helixi116 – 123Combined sources8
Helixi129 – 131Combined sources3
Helixi137 – 140Combined sources4
Helixi141 – 143Combined sources3
Helixi148 – 169Combined sources22
Helixi171 – 193Combined sources23
Helixi201 – 217Combined sources17
Helixi219 – 221Combined sources3
Helixi227 – 241Combined sources15
Turni242 – 245Combined sources4
Turni247 – 249Combined sources3
Helixi251 – 270Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IC8X-ray2.10A91-272[»]
2IRVX-ray2.30A/B92-273[»]
2LEPNMR-A1-61[»]
2NRFX-ray2.60A/B91-272[»]
2O7LX-ray2.50A93-272[»]
2XOVX-ray1.65A91-271[»]
2XOWX-ray2.09A92-270[»]
2XTUX-ray1.85A91-271[»]
2XTVX-ray1.70A93-272[»]
3B44X-ray1.70A91-270[»]
3B45X-ray1.90A91-270[»]
3TXTX-ray2.30A92-270[»]
3UBBX-ray2.60A91-272[»]
3ZEBX-ray2.20A92-270[»]
3ZMHX-ray2.30A91-270[»]
3ZMIX-ray2.20A92-270[»]
3ZMJX-ray2.30A92-270[»]
3ZOTX-ray2.40A92-271[»]
4H1DX-ray2.90A92-270[»]
4HDDX-ray1.35A2-74[»]
4NJNX-ray2.40A87-276[»]
4NJPX-ray2.40A87-276[»]
5F5BX-ray2.30A87-276[»]
5F5DX-ray2.50A87-276[»]
5F5GX-ray2.30A87-276[»]
5F5JX-ray2.40A87-276[»]
5F5KX-ray2.40A87-276[»]
5MT6X-ray2.16A91-270[»]
5MT7X-ray2.05A91-271[»]
5MT8X-ray1.95A92-270[»]
ProteinModelPortaliP09391
SMRiP09391
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09391

Family & Domainsi

Domaini

The loop between transmembrane domains 5 and 6 is flexible and may readily open to the extracellular side to allow water entry into the active site cavity.1 Publication

Sequence similaritiesi

Belongs to the peptidase S54 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EBW Bacteria
COG0705 LUCA
HOGENOMiHOG000269640
InParanoidiP09391
KOiK02441
OMAiHFSAMHI
PhylomeDBiP09391

Family and domain databases

Gene3Di1.20.1540.10, 1 hit
3.30.70.2350, 1 hit
HAMAPiMF_01594 Rhomboid_GlpG, 1 hit
InterProiView protein in InterPro
IPR038236 GlpG_N_sf
IPR022732 Peptidase_S54_GlpG_N
IPR002610 Peptidase_S54_rhomboid
IPR022764 Peptidase_S54_rhomboid_dom
IPR035952 Rhomboid-like_sf
IPR023662 Rhomboid_protease_GlpG
PANTHERiPTHR22936 PTHR22936, 1 hit
PfamiView protein in Pfam
PF01694 Rhomboid, 1 hit
PF12122 Rhomboid_N, 1 hit
TIGRFAMsiTIGR04239 rhombo_GlpG, 1 hit

Sequencei

Sequence statusi: Complete.

P09391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMITSFANP RVAQAFVDYM ATQGVILTIQ QHNQSDVWLA DESQAERVRA
60 70 80 90 100
ELARFLENPA DPRYLAASWQ AGHTGSGLHY RRYPFFAALR ERAGPVTWVM
110 120 130 140 150
MIACVVVFIA MQILGDQEVM LWLAWPFDPT LKFEFWRYFT HALMHFSLMH
160 170 180 190 200
ILFNLLWWWY LGGAVEKRLG SGKLIVITLI SALLSGYVQQ KFSGPWFGGL
210 220 230 240 250
SGVVYALMGY VWLRGERDPQ SGIYLQRGLI IFALIWIVAG WFDLFGMSMA
260 270
NGAHIAGLAV GLAMAFVDSL NARKRK
Length:276
Mass (Da):31,307
Last modified:October 11, 2004 - v5
Checksum:i6F67374E609968FC
GO

Sequence cautioni

The sequence AAA58222 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51 – 52EL → DV in AAA23890 (PubMed:3045764).Curated2
Sequence conflicti51 – 52EL → DV in CAA30398 (PubMed:3045764).Curated2
Sequence conflicti51 – 52EL → DV in AAC28166 (PubMed:8955387).Curated2
Sequence conflicti51 – 52EL → DV in AAA58222 (PubMed:9278503).Curated2
Sequence conflicti178 – 179TL → RS in AAA23890 (PubMed:3045764).Curated2
Sequence conflicti178 – 179TL → RS in CAA30398 (PubMed:3045764).Curated2
Sequence conflicti193S → T in AAA23890 (PubMed:3045764).Curated1
Sequence conflicti193S → T in CAA30398 (PubMed:3045764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M54940 Genomic DNA Translation: AAA23890.1
X07520 Genomic DNA Translation: CAA30398.1
M96795 Genomic DNA Translation: AAC28166.1
U18997 Genomic DNA Translation: AAA58222.1 Different initiation.
U00096 Genomic DNA Translation: AAT48182.1
AP009048 Genomic DNA Translation: BAE77868.1
PIRiC65138 BVECGG
RefSeqiWP_000928723.1, NZ_LN832404.1
YP_026220.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48182; AAT48182; b3424
BAE77868; BAE77868; BAE77868
GeneIDi947936
KEGGiecj:JW5687
eco:b3424
PATRICifig|1411691.4.peg.3305

Similar proteinsi

Entry informationi

Entry nameiGLPG_ECOLI
AccessioniPrimary (citable) accession number: P09391
Secondary accession number(s): P76691, Q2M788, Q6BF32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 150 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health