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P09389 (MTBB_BPSPB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase SPBetaI

Short name=M.SPBetaI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase SPBetaI
OrganismBacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
Taxonomic identifier66797 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeSpbetalikevirus
Virus hostBacillus pumilus (Bacillus mesentericus) [TaxID: 1408]
Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length257 AA.
Sequence statusFragments.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme methylates the first cytosine within the sequences GGCC and GCNGC.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›257›257Modification methylase SPBetaI
PRO_0000087867

Regions

Domain4 – 257254SAM-dependent MTase C5-type

Sites

Active site781 By similarity

Experimental info

Non-adjacent residues143 – 1442
Non-terminal residue2571

Sequences

Sequence LengthMass (Da)Tools
P09389 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 33063418AD8187BB

FASTA25728,880
        10         20         30         40         50         60 
MSKLRVMSLF SGIGAFEAAL RNIGVDYELI GFSEIDKYAI KSYCAIHNVS ETLNVGDISK 

        70         80         90        100        110        120 
AKKDNIPYFD LLTSGFPCPT FSVAGGRDGM EYKCSNCSHE HLITYEDYKK GVKCPKCEAV 

       130        140        150        160        170        180 
SKAKDERGTL FFETALLAEE KKPLMVGHVD LKGHDAIKRV YSPEGLSPTL TTMGGGHREP 

       190        200        210        220        230        240 
KIAEKQKEVR AVLTPEREEK RQNGRRFKEN GEPAFTVNTI DRHGVAIGEY PKYKIRKLSP 

       250 
LECWRLQAFD DEDFEKA 

« Hide

References

[1]"DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences."
Tran-Betcke A., Behrens B., Noyer-Weidner M., Trautner T.A.
Gene 42:89-96(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M19513 Genomic DNA. Translation: AAA32601.1.
M19514 Genomic DNA. Translation: AAA32602.1.

3D structure databases

ProteinModelPortalP09389.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2833. M.SPBetaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 2 hits.
[Graphical view]
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTBB_BPSPB
AccessionPrimary (citable) accession number: P09389
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries