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P09389

- MTBB_BPSPB

UniProt

P09389 - MTBB_BPSPB

Protein

Modification methylase SPBetaI

Gene
N/A
Organism
Bacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    • Comment

    Functioni

    This enzyme methylates the first cytosine within the sequences GGCC and GCNGC.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei78 – 781PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi2833. M.SPBetaI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase SPBetaI (EC:2.1.1.37)
    Short name:
    M.SPBetaI
    Alternative name(s):
    Cytosine-specific methyltransferase SPBetaI
    OrganismiBacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
    Taxonomic identifieri66797 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeSpbetalikevirus
    Virus hostiBacillus pumilus (Bacillus mesentericus) [TaxID: 1408]
    Bacillus subtilis [TaxID: 1423]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›257›257Modification methylase SPBetaIPRO_0000087867Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP09389.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 257254SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 2 hits.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 3 hits.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragments.

    P09389-1 [UniParc]FASTAAdd to Basket

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    MSKLRVMSLF SGIGAFEAAL RNIGVDYELI GFSEIDKYAI KSYCAIHNVS    50
    ETLNVGDISK AKKDNIPYFD LLTSGFPCPT FSVAGGRDGM EYKCSNCSHE 100
    HLITYEDYKK GVKCPKCEAV SKAKDERGTL FFETALLAEE KKPLMVGHVD 150
    LKGHDAIKRV YSPEGLSPTL TTMGGGHREP KIAEKQKEVR AVLTPEREEK 200
    RQNGRRFKEN GEPAFTVNTI DRHGVAIGEY PKYKIRKLSP LECWRLQAFD 250
    DEDFEKA 257
    Length:257
    Mass (Da):28,880
    Last modified:July 1, 1989 - v1
    Checksum:i33063418AD8187BB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi143 – 1442Curated
    Non-terminal residuei257 – 2571

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19513 Genomic DNA. Translation: AAA32601.1.
    M19514 Genomic DNA. Translation: AAA32602.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19513 Genomic DNA. Translation: AAA32601.1 .
    M19514 Genomic DNA. Translation: AAA32602.1 .

    3D structure databases

    ProteinModelPortali P09389.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 2833. M.SPBetaI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 3 hits.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences."
      Tran-Betcke A., Behrens B., Noyer-Weidner M., Trautner T.A.
      Gene 42:89-96(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiMTBB_BPSPB
    AccessioniPrimary (citable) accession number: P09389
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3