P09389 (MTBB_BPSPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Modification methylase SPBetaI Short name=M.SPBetaI EC=2.1.1.37 Alternative name(s): Cytosine-specific methyltransferase SPBetaI
Organism	Bacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
Taxonomic identifier	66797 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Spbetalikevirus
Virus host	Bacillus pumilus (Bacillus mesentericus) [TaxID: 1408] Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length	257 AA.
Sequence status	Fragments.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme methylates the first cytosine within the sequences GGCC and GCNGC.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›257	›257	Modification methylase SPBetaI		PRO_0000087867
Sites
	Active site	78	1	By similarity
Experimental info
	Non-adjacent residues	143 – 144	2
	Non-terminal residue	257	1

Sequences

	Sequence		Length	Mass (Da)
	P09389 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 33063418AD8187BB	FASTA	257	28,880
	10 20 30 40 50 60 MSKLRVMSLF SGIGAFEAAL RNIGVDYELI GFSEIDKYAI KSYCAIHNVS ETLNVGDISK 70 80 90 100 110 120 AKKDNIPYFD LLTSGFPCPT FSVAGGRDGM EYKCSNCSHE HLITYEDYKK GVKCPKCEAV 130 140 150 160 170 180 SKAKDERGTL FFETALLAEE KKPLMVGHVD LKGHDAIKRV YSPEGLSPTL TTMGGGHREP 190 200 210 220 230 240 KIAEKQKEVR AVLTPEREEK RQNGRRFKEN GEPAFTVNTI DRHGVAIGEY PKYKIRKLSP 250 LECWRLQAFD DEDFEKA

References

[1]	"DNA methyltransferase genes of Bacillus subtilis phages: comparison of their nucleotide sequences." Tran-Betcke A., Behrens B., Noyer-Weidner M., Trautner T.A. Gene 42:89-96(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19513 Genomic DNA. Translation: AAA32601.1. M19514 Genomic DNA. Translation: AAA32602.1.
3D structure databases
ProteinModelPortal	P09389.
ModBase	Search...
Protein family/group databases
REBASE	2833. M.SPBetaI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 2 hits. [Graphical view]
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTBB_BPSPB

Accession

Primary (citable) accession number: P09389

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program