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Protein

Modification methylase SPBetaI

Gene
N/A
Organism
Bacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This enzyme methylates the first cytosine within the sequences GGCC and GCNGC.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi2833. M.SPBetaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SPBetaI (EC:2.1.1.37)
Short name:
M.SPBetaI
Alternative name(s):
Cytosine-specific methyltransferase SPBetaI
OrganismiBacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
Taxonomic identifieri66797 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeSpbetavirus
Virus hostiBacillus pumilus (Bacillus mesentericus) [TaxID: 1408]
Bacillus subtilis [TaxID: 1423]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878671 – ›257Modification methylase SPBetaIAdd BLAST›257

Structurei

3D structure databases

ProteinModelPortaliP09389.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 257SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 2 hits.
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.

Sequencei

Sequence statusi: Fragments.

P09389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLRVMSLF SGIGAFEAAL RNIGVDYELI GFSEIDKYAI KSYCAIHNVS
60 70 80 90 100
ETLNVGDISK AKKDNIPYFD LLTSGFPCPT FSVAGGRDGM EYKCSNCSHE
110 120 130 140 150
HLITYEDYKK GVKCPKCEAV SKAKDERGTL FFETALLAEE KKPLMVGHVD
160 170 180 190 200
LKGHDAIKRV YSPEGLSPTL TTMGGGHREP KIAEKQKEVR AVLTPEREEK
210 220 230 240 250
RQNGRRFKEN GEPAFTVNTI DRHGVAIGEY PKYKIRKLSP LECWRLQAFD

DEDFEKA
Length:257
Mass (Da):28,880
Last modified:July 1, 1989 - v1
Checksum:i33063418AD8187BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-adjacent residuesi143 – 144Curated2
Non-terminal residuei2571

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19513 Genomic DNA. Translation: AAA32601.1.
M19514 Genomic DNA. Translation: AAA32602.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19513 Genomic DNA. Translation: AAA32601.1.
M19514 Genomic DNA. Translation: AAA32602.1.

3D structure databases

ProteinModelPortaliP09389.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi2833. M.SPBetaI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiView protein in InterPro
IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF00145. DNA_methylase. 2 hits.
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiView protein in PROSITE
PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTBB_BPSPB
AccessioniPrimary (citable) accession number: P09389
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 12, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.