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Protein

Shiga-like toxin 2 subunit B

Gene

stxB2

Organism
Enterobacteria phage 933W (Bacteriophage 933W)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionToxin, Viral exotoxin
Biological processModulation of host virulence by virus, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Shiga-like toxin 2 subunit B
Short name:
SLT-2 B subunit
Short name:
SLT-2b
Short name:
SLT-IIb
Alternative name(s):
Verocytotoxin 2 subunit B
Short name:
Verotoxin 2 subunit B
Gene namesi
Name:stxB2
Synonyms:stx2B
Ordered Locus Names:L0104
OrganismiEnterobacteria phage 933W (Bacteriophage 933W)
Taxonomic identifieri10730 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeSepvirinaeNona33virus
Virus hostiEscherichia coli O157:H7 [TaxID: 83334]
Proteomesi
  • UP000002135 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51R → C: Complete loss of cytotoxicity. 1 Publication1
Mutagenesisi61A → T: Complete loss of cytotoxicity. 1 Publication1
Mutagenesisi78G → D: Complete loss of cytotoxicity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003079520 – 89Shiga-like toxin 2 subunit BAdd BLAST70

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 75

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP09386

Interactioni

Subunit structurei

Shiga-like toxin contains a single subunit A and five copies of subunit B.

Protein-protein interaction databases

IntActiP09386, 1 interactor

Structurei

Secondary structure

189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 25Combined sources5
Beta strandi27 – 32Combined sources6
Beta strandi38 – 42Combined sources5
Beta strandi45 – 49Combined sources5
Helixi54 – 64Combined sources11
Beta strandi67 – 71Combined sources5
Beta strandi83 – 87Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77B/C/D/E/F20-89[»]
2GA4X-ray1.80B/C/D/E/F20-89[»]
6FE4X-ray3.00A/B/C/D/E20-89[»]
ProteinModelPortaliP09386
SMRiP09386
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09386

Family & Domainsi

Domaini

There are three Gb3-binding sites in each subunit B monomer, allowing for a tighter binding to the target cell. Binding sites 1 and 2 have higher binding affinities than site 3.

Sequence similaritiesi

Belongs to the stxB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK11007
OrthoDBiVOG090001P5

Family and domain databases

InterProiView protein in InterPro
IPR008992 Enterotoxin
IPR003189 SLT_beta
PfamiView protein in Pfam
PF02258 SLT_beta, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006042 SLT_beta, 1 hit
SUPFAMiSSF50203 SSF50203, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMFMAVLF ALASVNAMAA DCAKGKIEFS KYNEDDTFTV KVDGKEYWTS
60 70 80
RWNLQPLLQS AQLTGMTVTI KSSTCESGSG FAEVQFNND
Length:89
Mass (Da):9,874
Last modified:July 1, 1989 - v1
Checksum:i0EFEDB5B7CC581D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA Translation: CAA30715.1
Y10775 Genomic DNA Translation: CAA71748.1
AF125520 Genomic DNA Translation: AAD25446.1
PIRiS01033
RefSeqiNP_049501.1, NC_000924.1

Genome annotation databases

GeneIDi1262010
KEGGivg:1262010

Similar proteinsi

Entry informationi

Entry nameiSTXB_BP933
AccessioniPrimary (citable) accession number: P09386
Secondary accession number(s): Q96198
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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