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Protein

Shiga-like toxin 2 subunit B

Gene

stxB2

Organism
Enterobacteria phage 933W (Bacteriophage 933W)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Shiga-like toxin 2 subunit B
Short name:
SLT-2 B subunit
Short name:
SLT-2b
Short name:
SLT-IIb
Alternative name(s):
Verocytotoxin 2 subunit B
Short name:
Verotoxin 2 subunit B
Gene namesi
Name:stxB2
Synonyms:stx2B
Ordered Locus Names:L0104
OrganismiEnterobacteria phage 933W (Bacteriophage 933W)
Taxonomic identifieri10730 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridae
Virus hostiEscherichia coli O157:H7 [TaxID: 83334]
Proteomesi
  • UP000002135 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 511R → C: Complete loss of cytotoxicity. 1 Publication
Mutagenesisi61 – 611A → T: Complete loss of cytotoxicity. 1 Publication
Mutagenesisi78 – 781G → D: Complete loss of cytotoxicity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 8970Shiga-like toxin 2 subunit BPRO_0000030795Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi22 ↔ 75

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Shiga-like toxin contains a single subunit A and five copies of subunit B.

Protein-protein interaction databases

IntActiP09386. 1 interaction.

Structurei

Secondary structure

1
89
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi21 – 255Combined sources
Beta strandi27 – 326Combined sources
Beta strandi38 – 425Combined sources
Beta strandi45 – 495Combined sources
Helixi54 – 6411Combined sources
Beta strandi67 – 715Combined sources
Beta strandi83 – 875Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77B/C/D/E/F20-89[»]
2GA4X-ray1.80B/C/D/E/F20-89[»]
ProteinModelPortaliP09386.
SMRiP09386. Positions 20-89.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09386.

Family & Domainsi

Domaini

There are three Gb3-binding sites in each subunit B monomer, allowing for a tighter binding to the target cell. Binding sites 1 and 2 have higher binding affinities than site 3.

Sequence similaritiesi

Belongs to the stxB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK11007.

Family and domain databases

Gene3Di2.40.50.70. 1 hit.
InterProiIPR008992. Enterotoxin.
IPR003189. SLT_beta.
[Graphical view]
PfamiPF02258. SLT_beta. 1 hit.
[Graphical view]
ProDomiPD006042. SLT_beta. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50203. SSF50203. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMFMAVLF ALASVNAMAA DCAKGKIEFS KYNEDDTFTV KVDGKEYWTS
60 70 80
RWNLQPLLQS AQLTGMTVTI KSSTCESGSG FAEVQFNND
Length:89
Mass (Da):9,874
Last modified:July 1, 1989 - v1
Checksum:i0EFEDB5B7CC581D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA. Translation: CAA30715.1.
Y10775 Genomic DNA. Translation: CAA71748.1.
AF125520 Genomic DNA. Translation: AAD25446.1.
PIRiS01033.
RefSeqiNP_049501.1. NC_000924.1.

Genome annotation databases

GeneIDi1262010.
KEGGivg:1262010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA. Translation: CAA30715.1.
Y10775 Genomic DNA. Translation: CAA71748.1.
AF125520 Genomic DNA. Translation: AAD25446.1.
PIRiS01033.
RefSeqiNP_049501.1. NC_000924.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77B/C/D/E/F20-89[»]
2GA4X-ray1.80B/C/D/E/F20-89[»]
ProteinModelPortaliP09386.
SMRiP09386. Positions 20-89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09386. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1262010.
KEGGivg:1262010.

Phylogenomic databases

KOiK11007.

Miscellaneous databases

EvolutionaryTraceiP09386.

Family and domain databases

Gene3Di2.40.50.70. 1 hit.
InterProiIPR008992. Enterotoxin.
IPR003189. SLT_beta.
[Graphical view]
PfamiPF02258. SLT_beta. 1 hit.
[Graphical view]
ProDomiPD006042. SLT_beta. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50203. SSF50203. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli 933."
    Jackson M.P., Neill R.J., O'Brien A.D., Holmes R.K., Newland J.W.
    FEMS Microbiol. Lett. 44:109-114(1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "An ileX tRNA gene is located close to the Shiga toxin II operon in enterohemorrhagic Escherichia coli O157 and non-O157 strains."
    Schmidt H., Scheef J., Janetzki-Mittmann C., Datz M., Karch H.
    FEMS Microbiol. Lett. 149:39-44(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product."
    Plunkett G. III, Rose D.J., Durfee T.J., Blattner F.R.
    J. Bacteriol. 181:1767-1778(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Identification of three amino acid residues in the B subunit of Shiga toxin and Shiga-like toxin type II that are essential for holotoxin activity."
    Perera L.P., Samuel J.E., Holmes R.K., O'Brien A.D.
    J. Bacteriol. 173:1151-1160(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-51; ALA-61 AND GLY-78.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 20-89.
  6. "Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7."
    Fraser M.E., Cherney M.M., Marcato P., Mulvey G.L., Armstrong G.D., James M.N.
    Acta Crystallogr. F 62:627-630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 20-89.

Entry informationi

Entry nameiSTXB_BP933
AccessioniPrimary (citable) accession number: P09386
Secondary accession number(s): Q96198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: December 9, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.