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Protein

Shiga-like toxin 2 subunit B

Gene

stxB2

Organism
Enterobacteria phage 933W (Bacteriophage 933W)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Toxin, Viral exotoxin

Keywords - Biological processi

Modulation of host virulence by virus, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Shiga-like toxin 2 subunit B
Short name:
SLT-2 B subunit
Short name:
SLT-2b
Short name:
SLT-IIb
Alternative name(s):
Verocytotoxin 2 subunit B
Short name:
Verotoxin 2 subunit B
Gene namesi
Name:stxB2
Synonyms:stx2B
Ordered Locus Names:L0104
OrganismiEnterobacteria phage 933W (Bacteriophage 933W)
Taxonomic identifieri10730 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridae
Virus hostiEscherichia coli O157:H7 [TaxID: 83334]
Proteomesi
  • UP000002135 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51R → C: Complete loss of cytotoxicity. 1 Publication1
Mutagenesisi61A → T: Complete loss of cytotoxicity. 1 Publication1
Mutagenesisi78G → D: Complete loss of cytotoxicity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003079520 – 89Shiga-like toxin 2 subunit BAdd BLAST70

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi22 ↔ 75

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Shiga-like toxin contains a single subunit A and five copies of subunit B.

Protein-protein interaction databases

IntActiP09386. 1 interactor.

Structurei

Secondary structure

189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 25Combined sources5
Beta strandi27 – 32Combined sources6
Beta strandi38 – 42Combined sources5
Beta strandi45 – 49Combined sources5
Helixi54 – 64Combined sources11
Beta strandi67 – 71Combined sources5
Beta strandi83 – 87Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77B/C/D/E/F20-89[»]
2GA4X-ray1.80B/C/D/E/F20-89[»]
ProteinModelPortaliP09386.
SMRiP09386.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09386.

Family & Domainsi

Domaini

There are three Gb3-binding sites in each subunit B monomer, allowing for a tighter binding to the target cell. Binding sites 1 and 2 have higher binding affinities than site 3.

Sequence similaritiesi

Belongs to the stxB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK11007.

Family and domain databases

Gene3Di2.40.50.70. 1 hit.
InterProiIPR008992. Enterotoxin.
IPR003189. SLT_beta.
[Graphical view]
PfamiPF02258. SLT_beta. 1 hit.
[Graphical view]
ProDomiPD006042. SLT_beta. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50203. SSF50203. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMFMAVLF ALASVNAMAA DCAKGKIEFS KYNEDDTFTV KVDGKEYWTS
60 70 80
RWNLQPLLQS AQLTGMTVTI KSSTCESGSG FAEVQFNND
Length:89
Mass (Da):9,874
Last modified:July 1, 1989 - v1
Checksum:i0EFEDB5B7CC581D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA. Translation: CAA30715.1.
Y10775 Genomic DNA. Translation: CAA71748.1.
AF125520 Genomic DNA. Translation: AAD25446.1.
PIRiS01033.
RefSeqiNP_049501.1. NC_000924.1.

Genome annotation databases

GeneIDi1262010.
KEGGivg:1262010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA. Translation: CAA30715.1.
Y10775 Genomic DNA. Translation: CAA71748.1.
AF125520 Genomic DNA. Translation: AAD25446.1.
PIRiS01033.
RefSeqiNP_049501.1. NC_000924.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77B/C/D/E/F20-89[»]
2GA4X-ray1.80B/C/D/E/F20-89[»]
ProteinModelPortaliP09386.
SMRiP09386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09386. 1 interactor.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1262010.
KEGGivg:1262010.

Phylogenomic databases

KOiK11007.

Miscellaneous databases

EvolutionaryTraceiP09386.

Family and domain databases

Gene3Di2.40.50.70. 1 hit.
InterProiIPR008992. Enterotoxin.
IPR003189. SLT_beta.
[Graphical view]
PfamiPF02258. SLT_beta. 1 hit.
[Graphical view]
ProDomiPD006042. SLT_beta. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50203. SSF50203. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSTXB_BP933
AccessioniPrimary (citable) accession number: P09386
Secondary accession number(s): Q96198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.