Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P09385

- STXA_BP933

UniProt

P09385 - STXA_BP933

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Shiga-like toxin 2 subunit A

Gene

stxA2

Organism
Enterobacteria phage 933W (Bacteriophage 933W)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891By similarity
Sitei272 – 2732Cleavage; by furinBy similarity

GO - Molecular functioni

  1. rRNA N-glycosylase activity Source: UniProtKB-EC

GO - Biological processi

  1. negative regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein synthesis inhibitor, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Shiga-like toxin 2 subunit A (EC:3.2.2.22)
Short name:
SLT-2 A subunit
Short name:
SLT-2a
Short name:
SLT-IIa
Alternative name(s):
Verocytotoxin 2 subunit A
Verotoxin 2 subunit A
rRNA N-glycosidase 2
Gene namesi
Name:stxA2
Synonyms:stx2A
Ordered Locus Names:L0103
OrganismiEnterobacteria phage 933W (Bacteriophage 933W)
Taxonomic identifieri10730 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridae
Virus hostiEscherichia coli O157:H7 [TaxID: 83334]
ProteomesiUP000002135: Genome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 319297Shiga-like toxin 2 subunit APRO_0000030792Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi263 ↔ 282

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Shiga-like toxin contains a single A subunit and multiple copies of a B subunit.

Protein-protein interaction databases

IntActiP09385. 1 interaction.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 285
Helixi32 – 4615
Beta strandi47 – 559
Beta strandi58 – 636
Beta strandi71 – 777
Beta strandi89 – 946
Turni95 – 973
Beta strandi100 – 1056
Turni106 – 1094
Beta strandi110 – 1134
Helixi115 – 1173
Beta strandi125 – 1295
Helixi136 – 1438
Helixi154 – 16613
Beta strandi169 – 1713
Helixi174 – 18613
Helixi188 – 1925
Helixi194 – 2018
Helixi202 – 2043
Helixi215 – 2228
Helixi224 – 2307
Helixi231 – 2333
Beta strandi240 – 2423
Beta strandi245 – 2473
Helixi250 – 2567
Beta strandi284 – 2874
Beta strandi290 – 2934
Beta strandi296 – 2994
Helixi300 – 3067
Helixi312 – 3176

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77A23-319[»]
2GA4X-ray1.80A23-319[»]
ProteinModelPortaliP09385.
SMRiP09385. Positions 23-319.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09385.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 272250A1By similarityAdd
BLAST
Regioni273 – 31442A2By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR016331. Shiga-like_toxin_subunit_A.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PIRSFiPIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09385-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKCILFKWVL CLLLGFSSVS YSREFTIDFS TQQSYVSSLN SIRTEISTPL
60 70 80 90 100
EHISQGTTSV SVINHTPPGS YFAVDIRGLD VYQARFDHLR LIIEQNNLYV
110 120 130 140 150
AGFVNTATNT FYRFSDFTHI SVPGVTTVSM TTDSSYTTLQ RVAALERSGM
160 170 180 190 200
QISRHSLVSS YLALMEFSGN TMTRDASRAV LRFVTVTAEA LRFRQIQREF
210 220 230 240 250
RQALSETAPV YTMTPGDVDL TLNWGRISNV LPEYRGEDGV RVGRISFNNI
260 270 280 290 300
SAILGTVAVI LNCHHQGARS VRAVNEESQP ECQITGDRPV IKINNTLWES
310
NTAAAFLNRK SQFLYTTGK
Length:319
Mass (Da):35,714
Last modified:February 1, 1996 - v2
Checksum:i98F73319ACAE48D6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171S → P in strain: OX3:H21.
Natural varianti26 – 261T → M in strain: OX3:H21.
Natural varianti277 – 2771E → D in strain: FLY16 and CS1718.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07865 Genomic DNA. Translation: CAA30714.1.
M59432 Unassigned DNA. Translation: AAA19623.1.
L11079 Unassigned DNA. Translation: AAA16362.1.
Y10775 Genomic DNA. Translation: CAA71747.1.
AB017524 Genomic DNA. Translation: BAA33759.1.
AB015057 Genomic DNA. Translation: BAA34372.1.
AF461167 Genomic DNA. Translation: AAM70033.1.
AB048239 Genomic DNA. Translation: BAB83026.1.
AB048240 Genomic DNA. Translation: BAB83028.1.
AY443052 Genomic DNA. Translation: AAS07596.1.
AF125520 Genomic DNA. Translation: AAD25445.1.
PIRiI76713.
S01032.
RefSeqiNP_049500.1. NC_000924.1.

Genome annotation databases

GeneIDi1261950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07865 Genomic DNA. Translation: CAA30714.1 .
M59432 Unassigned DNA. Translation: AAA19623.1 .
L11079 Unassigned DNA. Translation: AAA16362.1 .
Y10775 Genomic DNA. Translation: CAA71747.1 .
AB017524 Genomic DNA. Translation: BAA33759.1 .
AB015057 Genomic DNA. Translation: BAA34372.1 .
AF461167 Genomic DNA. Translation: AAM70033.1 .
AB048239 Genomic DNA. Translation: BAB83026.1 .
AB048240 Genomic DNA. Translation: BAB83028.1 .
AY443052 Genomic DNA. Translation: AAS07596.1 .
AF125520 Genomic DNA. Translation: AAD25445.1 .
PIRi I76713.
S01032.
RefSeqi NP_049500.1. NC_000924.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1R4P X-ray 1.77 A 23-319 [» ]
2GA4 X-ray 1.80 A 23-319 [» ]
ProteinModelPortali P09385.
SMRi P09385. Positions 23-319.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P09385. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1261950.

Miscellaneous databases

EvolutionaryTracei P09385.

Family and domain databases

Gene3Di 3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProi IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR016331. Shiga-like_toxin_subunit_A.
[Graphical view ]
Pfami PF00161. RIP. 1 hit.
[Graphical view ]
PIRSFi PIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAMi SSF56371. SSF56371. 1 hit.
PROSITEi PS00275. SHIGA_RICIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli 933."
    Jackson M.P., Neill R.J., O'Brien A.D., Holmes R.K., Newland J.W.
    FEMS Microbiol. Lett. 44:109-114(1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157:H-strain E32511."
    Schmitt C.K., McKee M.L., O'Brien A.D.
    Infect. Immun. 59:1065-1073(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: E32511.
  3. "Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons."
    Paton A.W., Paton J.C., Manning P.A.
    Microb. Pathog. 15:77-82(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: OX3:H21.
  4. "An ileX tRNA gene is located close to the Shiga toxin II operon in enterohemorrhagic Escherichia coli O157 and non-O157 strains."
    Schmidt H., Scheef J., Janetzki-Mittmann C., Datz M., Karch H.
    FEMS Microbiol. Lett. 149:39-44(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Identification of an insertion sequence, IS1203 variant, in a Shiga toxin 2 gene of Escherichia coli O157:H7."
    Kusumoto M., Nishiya Y., Kawamura Y., Shinagawa K.
    J. Biosci. Bioeng. 87:93-96(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Detection of Escherichia coli O157:H7 from Musca domestica (Diptera: Muscidae) at a cattle farm in Japan."
    Iwasa M., Makino S., Asakura H., Kobori H., Morimoto Y.
    J. Med. Entomol. 36:108-112(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: FLY16.
  7. "Characterization of Shiga toxin genes in Shiga toxin-producing Escherichia coli isolated in Korea."
    Yu J.Y., Jeon H.G., Kang Y.H., Kim E.C., Sohn C.K., Lee B.K.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CS1718.
  8. "Phylogenetic diversity and similarity of active sites of Shiga toxin (stx) in Shiga toxin-producing Escherichia coli (STEC) isolates from humans and animals."
    Asakura H., Makino S., Kobori H., Watarai M., Shirahata T., Ikeda T., Takeshi K.
    Epidemiol. Infect. 127:27-36(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  9. "Genetic typing of shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis."
    De Baets L., Van der Taelen I., De Filette M., Pierard D., Allison L., De Greve H., Hernalsteens J.P., Imberechts H.
    Appl. Environ. Microbiol. 70:6309-6314(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  10. "Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product."
    Plunkett G. III, Rose D.J., Durfee T.J., Blattner F.R.
    J. Bacteriol. 181:1767-1778(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  11. "Regulation of the Shiga-like toxin II operon in Escherichia coli."
    Muhldorfer I., Hacker J., Keusch G.T., Acheson D.W., Tschape H., Kane A.V., Ritter A., Olschlager T., Donohue-Rolfe A.
    Infect. Immun. 64:495-502(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. Cited for: X-RAY CRYSTALLOGRAPHY (1.77 ANGSTROMS) OF 23-319.

Entry informationi

Entry nameiSTXA_BP933
AccessioniPrimary (citable) accession number: P09385
Secondary accession number(s): Q9R398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3