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Protein

Shiga-like toxin 2 subunit A

Gene

stxA2

Organism
Enterobacteria phage 933W (Bacteriophage 933W)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein synthesis inhibitor, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Shiga-like toxin 2 subunit A (EC:3.2.2.22)
Short name:
SLT-2 A subunit
Short name:
SLT-2a
Short name:
SLT-IIa
Alternative name(s):
Verocytotoxin 2 subunit A
Verotoxin 2 subunit A
rRNA N-glycosidase 2
Gene namesi
Name:stxA2
Synonyms:stx2A
Ordered Locus Names:L0103
OrganismiEnterobacteria phage 933W (Bacteriophage 933W)
Taxonomic identifieri10730 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridae
Virus hostiEscherichia coli O157:H7 [TaxID: 83334]
Proteomesi
  • UP000002135 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 319297Shiga-like toxin 2 subunit APRO_0000030792Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi263 ↔ 282

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei272 – 2732Cleavage; by furinBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Shiga-like toxin contains a single A subunit and multiple copies of a B subunit.

Protein-protein interaction databases

IntActiP09385. 1 interaction.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 285Combined sources
Helixi32 – 4615Combined sources
Beta strandi47 – 559Combined sources
Beta strandi58 – 636Combined sources
Beta strandi71 – 777Combined sources
Beta strandi89 – 946Combined sources
Turni95 – 973Combined sources
Beta strandi100 – 1056Combined sources
Turni106 – 1094Combined sources
Beta strandi110 – 1134Combined sources
Helixi115 – 1173Combined sources
Beta strandi125 – 1295Combined sources
Helixi136 – 1438Combined sources
Helixi154 – 16613Combined sources
Beta strandi169 – 1713Combined sources
Helixi174 – 18613Combined sources
Helixi188 – 1925Combined sources
Helixi194 – 2018Combined sources
Helixi202 – 2043Combined sources
Helixi215 – 2228Combined sources
Helixi224 – 2307Combined sources
Helixi231 – 2333Combined sources
Beta strandi240 – 2423Combined sources
Beta strandi245 – 2473Combined sources
Helixi250 – 2567Combined sources
Beta strandi284 – 2874Combined sources
Beta strandi290 – 2934Combined sources
Beta strandi296 – 2994Combined sources
Helixi300 – 3067Combined sources
Helixi312 – 3176Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77A23-319[»]
2GA4X-ray1.80A23-319[»]
ProteinModelPortaliP09385.
SMRiP09385. Positions 23-319.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09385.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 272250A1By similarityAdd
BLAST
Regioni273 – 31442A2By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK11006.

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR016331. Shiga-like_toxin_subunit_A.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PIRSFiPIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCILFKWVL CLLLGFSSVS YSREFTIDFS TQQSYVSSLN SIRTEISTPL
60 70 80 90 100
EHISQGTTSV SVINHTPPGS YFAVDIRGLD VYQARFDHLR LIIEQNNLYV
110 120 130 140 150
AGFVNTATNT FYRFSDFTHI SVPGVTTVSM TTDSSYTTLQ RVAALERSGM
160 170 180 190 200
QISRHSLVSS YLALMEFSGN TMTRDASRAV LRFVTVTAEA LRFRQIQREF
210 220 230 240 250
RQALSETAPV YTMTPGDVDL TLNWGRISNV LPEYRGEDGV RVGRISFNNI
260 270 280 290 300
SAILGTVAVI LNCHHQGARS VRAVNEESQP ECQITGDRPV IKINNTLWES
310
NTAAAFLNRK SQFLYTTGK
Length:319
Mass (Da):35,714
Last modified:February 1, 1996 - v2
Checksum:i98F73319ACAE48D6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171S → P in strain: OX3:H21.
Natural varianti26 – 261T → M in strain: OX3:H21.
Natural varianti277 – 2771E → D in strain: FLY16 and CS1718.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA. Translation: CAA30714.1.
M59432 Unassigned DNA. Translation: AAA19623.1.
L11079 Unassigned DNA. Translation: AAA16362.1.
Y10775 Genomic DNA. Translation: CAA71747.1.
AB017524 Genomic DNA. Translation: BAA33759.1.
AB015057 Genomic DNA. Translation: BAA34372.1.
AF461167 Genomic DNA. Translation: AAM70033.1.
AB048239 Genomic DNA. Translation: BAB83026.1.
AB048240 Genomic DNA. Translation: BAB83028.1.
AY443052 Genomic DNA. Translation: AAS07596.1.
AF125520 Genomic DNA. Translation: AAD25445.1.
PIRiI76713.
S01032.
RefSeqiNP_049500.1. NC_000924.1.

Genome annotation databases

GeneIDi1261950.
KEGGivg:1261950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07865 Genomic DNA. Translation: CAA30714.1.
M59432 Unassigned DNA. Translation: AAA19623.1.
L11079 Unassigned DNA. Translation: AAA16362.1.
Y10775 Genomic DNA. Translation: CAA71747.1.
AB017524 Genomic DNA. Translation: BAA33759.1.
AB015057 Genomic DNA. Translation: BAA34372.1.
AF461167 Genomic DNA. Translation: AAM70033.1.
AB048239 Genomic DNA. Translation: BAB83026.1.
AB048240 Genomic DNA. Translation: BAB83028.1.
AY443052 Genomic DNA. Translation: AAS07596.1.
AF125520 Genomic DNA. Translation: AAD25445.1.
PIRiI76713.
S01032.
RefSeqiNP_049500.1. NC_000924.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R4PX-ray1.77A23-319[»]
2GA4X-ray1.80A23-319[»]
ProteinModelPortaliP09385.
SMRiP09385. Positions 23-319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09385. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261950.
KEGGivg:1261950.

Phylogenomic databases

KOiK11006.

Miscellaneous databases

EvolutionaryTraceiP09385.

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR016331. Shiga-like_toxin_subunit_A.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PIRSFiPIRSF001924. Shigella_toxin_subunit_A. 1 hit.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTXA_BP933
AccessioniPrimary (citable) accession number: P09385
Secondary accession number(s): Q9R398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.