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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HGPRT

Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine-guanine phosphoribosyltransferase (HGPRT)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei129GMPBy similarity1
Active sitei198Proton acceptorBy similarity1
Binding sitei226GMPBy similarity1
Metal bindingi253MagnesiumBy similarity1
Binding sitei253GMP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi194 – 202GMPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HGPRT
OrganismiSchistosoma mansoni (Blood fluke)
Taxonomic identifieri6183 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395941 – 284Hypoxanthine-guanine phosphoribosyltransferaseAdd BLAST284

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

Secondary structure

1284
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi64 – 66Combined sources3
Helixi76 – 78Combined sources3
Helixi83 – 85Combined sources3
Turni86 – 88Combined sources3
Beta strandi89 – 94Combined sources6
Helixi96 – 116Combined sources21
Beta strandi122 – 127Combined sources6
Turni128 – 131Combined sources4
Helixi132 – 147Combined sources16
Turni148 – 150Combined sources3
Beta strandi155 – 160Combined sources6
Beta strandi188 – 200Combined sources13
Helixi201 – 210Combined sources10
Beta strandi216 – 226Combined sources11
Beta strandi237 – 242Combined sources6
Beta strandi247 – 249Combined sources3
Beta strandi262 – 267Combined sources6
Helixi269 – 274Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IPFX-ray2.80A/B/C/D55-284[»]
ProteinModelPortaliP09383.
SMRiP09383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3367. Eukaryota.
COG0634. LUCA.
HOGENOMiHOG000236521.
OrthoDBiEOG091G0K6K.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
InterProiView protein in InterPro
IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiView protein in PROSITE
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.

Sequencei

Sequence statusi: Complete.

P09383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTSLITSST TVTLTLSQIY YILDIACGFL ISVLVWMNSS VLDNGNHSNP
60 70 80 90 100
QIRDMSSNMI KADCVVIEDS FRGFPTEYFC TSPRYDECLD YVLIPNGMIK
110 120 130 140 150
DRLEKMSMDI VDYYEACNAT SITLMCVLKG GFKFLADLVD GLERTVRARG
160 170 180 190 200
IVLPMSVEFV RVKSYVNDVS IHEPILTGLG DPSEYKDKNV LVVEDIIDTG
210 220 230 240 250
KTITKLISHL DSLSTKSVKV ASLLVKRTSP RNDYRPDVGF EVPNRFVVGY
260 270 280
ALDYNDNFRD LHHICVINEV GQKKFSVPCT SKPV
Length:284
Mass (Da):31,854
Last modified:July 1, 1989 - v1
Checksum:i5C2492D6ADFC6654
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07883 mRNA. Translation: CAA30730.1. Sequence problems.
X13531
, X13532, X13533, X13534 Genomic DNA. Translation: CAA31885.1.
PIRiS04278.
S09614.

Genome annotation databases

GeneDBiSmp_103560.1:pep.

Similar proteinsi

Entry informationi

Entry nameiHPRT_SCHMA
AccessioniPrimary (citable) accession number: P09383
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: August 30, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families