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P09383

- HPRT_SCHMA

UniProt

P09383 - HPRT_SCHMA

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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HGPRT

Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei129 – 1291GMPBy similarity
Active sitei198 – 1981Proton acceptorBy similarity
Binding sitei226 – 2261GMPBy similarity
Metal bindingi253 – 2531MagnesiumBy similarity
Binding sitei253 – 2531GMP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi194 – 2029GMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HGPRT
OrganismiSchistosoma mansoni (Blood fluke)
Taxonomic identifieri6183 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139594Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP09383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000236521.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09383-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTSLITSST TVTLTLSQIY YILDIACGFL ISVLVWMNSS VLDNGNHSNP
60 70 80 90 100
QIRDMSSNMI KADCVVIEDS FRGFPTEYFC TSPRYDECLD YVLIPNGMIK
110 120 130 140 150
DRLEKMSMDI VDYYEACNAT SITLMCVLKG GFKFLADLVD GLERTVRARG
160 170 180 190 200
IVLPMSVEFV RVKSYVNDVS IHEPILTGLG DPSEYKDKNV LVVEDIIDTG
210 220 230 240 250
KTITKLISHL DSLSTKSVKV ASLLVKRTSP RNDYRPDVGF EVPNRFVVGY
260 270 280
ALDYNDNFRD LHHICVINEV GQKKFSVPCT SKPV
Length:284
Mass (Da):31,854
Last modified:July 1, 1989 - v1
Checksum:i5C2492D6ADFC6654
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07883 mRNA. Translation: CAA30730.1. Sequence problems.
X13531
, X13532, X13533, X13534 Genomic DNA. Translation: CAA31885.1.
PIRiS04278.
S09614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07883 mRNA. Translation: CAA30730.1 . Sequence problems.
X13531
, X13532 , X13533 , X13534 Genomic DNA. Translation: CAA31885.1 .
PIRi S04278.
S09614.

3D structure databases

ProteinModelPortali P09383.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOGENOMi HOG000236521.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of cDNA encoding the hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) of Schistosoma mansoni; a putative target for chemotherapy."
    Craig S.P., McKerrow J.H., Newport G.N., Wang C.C.
    Nucleic Acids Res. 16:7087-7101(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Puerto Rican.
  2. "Evidence for a class of very small introns in the gene for hypoxanthine-guanine phosphoribosyltransferase in Schistosoma mansoni."
    Craig S.P., Muralidhar M.G., McKerrow J.H., Wang C.C.
    Nucleic Acids Res. 17:1635-1647(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Puerto Rican.

Entry informationi

Entry nameiHPRT_SCHMA
AccessioniPrimary (citable) accession number: P09383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3