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Protein

HTH-type transcriptional activator RhaS

Gene

rhaS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates expression of the rhaBAD and rhaT operons.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi190 – 20920H-T-H motifBy similarityAdd
BLAST
DNA bindingi239 – 26224H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  • core promoter proximal region sequence-specific DNA binding Source: GO_Central
  • DNA binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Rhamnose metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00221.
ECOL316407:JW3876-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional activator RhaS
Alternative name(s):
L-rhamnose operon regulatory protein RhaS
Gene namesi
Name:rhaS
Synonyms:rhaC2
Ordered Locus Names:b3905, JW3876
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10843. rhaS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi201 – 2011L → A: 48% of wild-type activity. 1 Publication
Mutagenesisi202 – 2021R → A: 4% of wild-type activity. 1 Publication
Mutagenesisi205 – 2051H → A: 28% of wild-type activity. 1 Publication
Mutagenesisi206 – 2061R → A: 0.1% of wild-type activity. 1 Publication
Mutagenesisi246 – 2461C → A: 66% of wild-type activity. 1 Publication
Mutagenesisi247 – 2471G → A: 6.2% of wild-type activity. 1 Publication
Mutagenesisi247 – 2471G → R: 0.9% of wild-type activity. 1 Publication
Mutagenesisi248 – 2481F → A: 0.9% of wild-type activity. 1 Publication
Mutagenesisi248 – 2481F → V: 3.3% of wild-type activity. 1 Publication
Mutagenesisi249 – 2491S → G: 93% of wild-type activity. 1 Publication
Mutagenesisi250 – 2501D → A: 8.3% of wild-type activity. 1 Publication
Mutagenesisi251 – 2511S → G: 87% of wild-type activity. 1 Publication
Mutagenesisi252 – 2521N → A: 31% of wild-type activity. 1 Publication
Mutagenesisi252 – 2521N → H: 0.4% of wild-type activity. 1 Publication
Mutagenesisi252 – 2521N → I: 0.3% of wild-type activity. 1 Publication
Mutagenesisi252 – 2521N → S: 37% of wild-type activity. 1 Publication
Mutagenesisi253 – 2531H → A: 48% of wild-type activity. 1 Publication
Mutagenesisi253 – 2531H → Q: 32% of wild-type activity. 1 Publication
Mutagenesisi254 – 2541F → A: 1.2% of wild-type activity. 1 Publication
Mutagenesisi255 – 2551S → A: 80% of wild-type activity. 1 Publication
Mutagenesisi256 – 2561T → A: 61% of wild-type activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278HTH-type transcriptional activator RhaSPRO_0000194563Add
BLAST

Proteomic databases

PaxDbiP09377.

Expressioni

Inductioni

Induced by RhaR in response to L-rhamnose. Binding of the cAMP receptor protein (CRP) is required for full expression.1 Publication

Interactioni

Subunit structurei

Binds DNA as a dimer.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei241 – 2411Interaction with sigma-70
Sitei250 – 2501Interaction with sigma-70

Protein-protein interaction databases

BioGridi4260998. 4 interactions.
DIPiDIP-10694N.
IntActiP09377. 24 interactions.
MINTiMINT-1305270.
STRINGi511145.b3905.

Structurei

3D structure databases

ProteinModelPortaliP09377.
SMRiP09377. Positions 169-273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107S4Z. Bacteria.
ENOG410XT8S. LUCA.
HOGENOMiHOG000290513.
InParanoidiP09377.
KOiK02855.
OMAiYAIAIEP.
PhylomeDBiP09377.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
HAMAPiMF_01534. HTH_type_RhaS. 1 hit.
InterProiIPR003313. AraC-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR020449. Tscrpt_reg_HTH_AraC-type.
IPR023609. Tscrpt_reg_HTH_RhaS.
[Graphical view]
PfamiPF02311. AraC_binding. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51215. SSF51215. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF
60 70 80 90 100
NGQPYTITGG TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ
110 120 130 140 150
LLPQELDGQY PSHWRVNHSV LQQVRQLVAQ MEQQEGENDL PSTASREILF
160 170 180 190 200
MQLLLLLRKS SLQENLENSA SRLNLLLAWL EDHFADEVNW DAVADQFSLS
210 220 230 240 250
LRTLHRQLKQ QTGLTPQRYL NRLRLMKARH LLRHSEASVT DIAYRCGFSD
260 270
SNHFSTLFRR EFNWSPRDIR QGRDGFLQ
Length:278
Mass (Da):32,315
Last modified:July 1, 1989 - v1
Checksum:i64264514484C0D2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06058 Genomic DNA. Translation: CAA29452.1.
L19201 Genomic DNA. Translation: AAB03038.1.
U00096 Genomic DNA. Translation: AAC76887.1.
AP009048 Genomic DNA. Translation: BAE77404.1.
PIRiS40849.
RefSeqiNP_418341.1. NC_000913.3.
WP_000217137.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76887; AAC76887; b3905.
BAE77404; BAE77404; BAE77404.
GeneIDi948397.
KEGGiecj:JW3876.
eco:b3905.
PATRICi32123321. VBIEscCol129921_4021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06058 Genomic DNA. Translation: CAA29452.1.
L19201 Genomic DNA. Translation: AAB03038.1.
U00096 Genomic DNA. Translation: AAC76887.1.
AP009048 Genomic DNA. Translation: BAE77404.1.
PIRiS40849.
RefSeqiNP_418341.1. NC_000913.3.
WP_000217137.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP09377.
SMRiP09377. Positions 169-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260998. 4 interactions.
DIPiDIP-10694N.
IntActiP09377. 24 interactions.
MINTiMINT-1305270.
STRINGi511145.b3905.

Proteomic databases

PaxDbiP09377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76887; AAC76887; b3905.
BAE77404; BAE77404; BAE77404.
GeneIDi948397.
KEGGiecj:JW3876.
eco:b3905.
PATRICi32123321. VBIEscCol129921_4021.

Organism-specific databases

EchoBASEiEB0836.
EcoGeneiEG10843. rhaS.

Phylogenomic databases

eggNOGiENOG4107S4Z. Bacteria.
ENOG410XT8S. LUCA.
HOGENOMiHOG000290513.
InParanoidiP09377.
KOiK02855.
OMAiYAIAIEP.
PhylomeDBiP09377.

Enzyme and pathway databases

BioCyciEcoCyc:PD00221.
ECOL316407:JW3876-MONOMER.

Miscellaneous databases

PROiP09377.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
HAMAPiMF_01534. HTH_type_RhaS. 1 hit.
InterProiIPR003313. AraC-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR020449. Tscrpt_reg_HTH_AraC-type.
IPR023609. Tscrpt_reg_HTH_RhaS.
[Graphical view]
PfamiPF02311. AraC_binding. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51215. SSF51215. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHAS_ECOLI
AccessioniPrimary (citable) accession number: P09377
Secondary accession number(s): Q2M8K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: September 7, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.