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Protein

HTH-type transcriptional activator RhaS

Gene

rhaS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates expression of the rhaBAD and rhaT operons.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi190 – 209H-T-H motifBy similarityAdd BLAST20
DNA bindingi239 – 262H-T-H motifBy similarityAdd BLAST24

GO - Molecular functioni

  • core promoter proximal region sequence-specific DNA binding Source: GO_Central
  • DNA binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Rhamnose metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00221.
ECOL316407:JW3876-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional activator RhaS
Alternative name(s):
L-rhamnose operon regulatory protein RhaS
Gene namesi
Name:rhaS
Synonyms:rhaC2
Ordered Locus Names:b3905, JW3876
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10843. rhaS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi201L → A: 48% of wild-type activity. 1 Publication1
Mutagenesisi202R → A: 4% of wild-type activity. 1 Publication1
Mutagenesisi205H → A: 28% of wild-type activity. 1 Publication1
Mutagenesisi206R → A: 0.1% of wild-type activity. 1 Publication1
Mutagenesisi246C → A: 66% of wild-type activity. 1 Publication1
Mutagenesisi247G → A: 6.2% of wild-type activity. 1 Publication1
Mutagenesisi247G → R: 0.9% of wild-type activity. 1 Publication1
Mutagenesisi248F → A: 0.9% of wild-type activity. 1 Publication1
Mutagenesisi248F → V: 3.3% of wild-type activity. 1 Publication1
Mutagenesisi249S → G: 93% of wild-type activity. 1 Publication1
Mutagenesisi250D → A: 8.3% of wild-type activity. 1 Publication1
Mutagenesisi251S → G: 87% of wild-type activity. 1 Publication1
Mutagenesisi252N → A: 31% of wild-type activity. 1 Publication1
Mutagenesisi252N → H: 0.4% of wild-type activity. 1 Publication1
Mutagenesisi252N → I: 0.3% of wild-type activity. 1 Publication1
Mutagenesisi252N → S: 37% of wild-type activity. 1 Publication1
Mutagenesisi253H → A: 48% of wild-type activity. 1 Publication1
Mutagenesisi253H → Q: 32% of wild-type activity. 1 Publication1
Mutagenesisi254F → A: 1.2% of wild-type activity. 1 Publication1
Mutagenesisi255S → A: 80% of wild-type activity. 1 Publication1
Mutagenesisi256T → A: 61% of wild-type activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001945631 – 278HTH-type transcriptional activator RhaSAdd BLAST278

Proteomic databases

PaxDbiP09377.
PRIDEiP09377.

Expressioni

Inductioni

Induced by RhaR in response to L-rhamnose. Binding of the cAMP receptor protein (CRP) is required for full expression.1 Publication

Interactioni

Subunit structurei

Binds DNA as a dimer.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei241Interaction with sigma-701
Sitei250Interaction with sigma-701

Protein-protein interaction databases

BioGridi4260998. 4 interactors.
DIPiDIP-10694N.
IntActiP09377. 24 interactors.
MINTiMINT-1305270.
STRINGi511145.b3905.

Structurei

3D structure databases

ProteinModelPortaliP09377.
SMRiP09377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107S4Z. Bacteria.
ENOG410XT8S. LUCA.
HOGENOMiHOG000290513.
InParanoidiP09377.
KOiK02855.
OMAiYAIAIEP.
PhylomeDBiP09377.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
HAMAPiMF_01534. HTH_type_RhaS. 1 hit.
InterProiIPR003313. AraC-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR020449. Tscrpt_reg_HTH_AraC-type.
IPR023609. Tscrpt_reg_HTH_RhaS.
[Graphical view]
PfamiPF02311. AraC_binding. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51215. SSF51215. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF
60 70 80 90 100
NGQPYTITGG TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ
110 120 130 140 150
LLPQELDGQY PSHWRVNHSV LQQVRQLVAQ MEQQEGENDL PSTASREILF
160 170 180 190 200
MQLLLLLRKS SLQENLENSA SRLNLLLAWL EDHFADEVNW DAVADQFSLS
210 220 230 240 250
LRTLHRQLKQ QTGLTPQRYL NRLRLMKARH LLRHSEASVT DIAYRCGFSD
260 270
SNHFSTLFRR EFNWSPRDIR QGRDGFLQ
Length:278
Mass (Da):32,315
Last modified:July 1, 1989 - v1
Checksum:i64264514484C0D2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06058 Genomic DNA. Translation: CAA29452.1.
L19201 Genomic DNA. Translation: AAB03038.1.
U00096 Genomic DNA. Translation: AAC76887.1.
AP009048 Genomic DNA. Translation: BAE77404.1.
PIRiS40849.
RefSeqiNP_418341.1. NC_000913.3.
WP_000217137.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76887; AAC76887; b3905.
BAE77404; BAE77404; BAE77404.
GeneIDi948397.
KEGGiecj:JW3876.
eco:b3905.
PATRICi32123321. VBIEscCol129921_4021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06058 Genomic DNA. Translation: CAA29452.1.
L19201 Genomic DNA. Translation: AAB03038.1.
U00096 Genomic DNA. Translation: AAC76887.1.
AP009048 Genomic DNA. Translation: BAE77404.1.
PIRiS40849.
RefSeqiNP_418341.1. NC_000913.3.
WP_000217137.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP09377.
SMRiP09377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260998. 4 interactors.
DIPiDIP-10694N.
IntActiP09377. 24 interactors.
MINTiMINT-1305270.
STRINGi511145.b3905.

Proteomic databases

PaxDbiP09377.
PRIDEiP09377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76887; AAC76887; b3905.
BAE77404; BAE77404; BAE77404.
GeneIDi948397.
KEGGiecj:JW3876.
eco:b3905.
PATRICi32123321. VBIEscCol129921_4021.

Organism-specific databases

EchoBASEiEB0836.
EcoGeneiEG10843. rhaS.

Phylogenomic databases

eggNOGiENOG4107S4Z. Bacteria.
ENOG410XT8S. LUCA.
HOGENOMiHOG000290513.
InParanoidiP09377.
KOiK02855.
OMAiYAIAIEP.
PhylomeDBiP09377.

Enzyme and pathway databases

BioCyciEcoCyc:PD00221.
ECOL316407:JW3876-MONOMER.

Miscellaneous databases

PROiP09377.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
HAMAPiMF_01534. HTH_type_RhaS. 1 hit.
InterProiIPR003313. AraC-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR020449. Tscrpt_reg_HTH_AraC-type.
IPR023609. Tscrpt_reg_HTH_RhaS.
[Graphical view]
PfamiPF02311. AraC_binding. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51215. SSF51215. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHAS_ECOLI
AccessioniPrimary (citable) accession number: P09377
Secondary accession number(s): Q2M8K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.