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Protein

HTH-type transcriptional activator RhaS

Gene

rhaS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates expression of the rhaBAD and rhaT operons.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi190 – 209H-T-H motifBy similarityAdd BLAST20
DNA bindingi239 – 262H-T-H motifBy similarityAdd BLAST24

GO - Molecular functioni

  • DNA binding transcription factor activity Source: EcoCyc
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • rhamnose metabolic process Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processRhamnose metabolism, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00221

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional activator RhaS
Alternative name(s):
L-rhamnose operon regulatory protein RhaS
Gene namesi
Name:rhaS
Synonyms:rhaC2
Ordered Locus Names:b3905, JW3876
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10843 rhaS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi201L → A: 48% of wild-type activity. 1 Publication1
Mutagenesisi202R → A: 4% of wild-type activity. 1 Publication1
Mutagenesisi205H → A: 28% of wild-type activity. 1 Publication1
Mutagenesisi206R → A: 0.1% of wild-type activity. 1 Publication1
Mutagenesisi246C → A: 66% of wild-type activity. 1 Publication1
Mutagenesisi247G → A: 6.2% of wild-type activity. 1 Publication1
Mutagenesisi247G → R: 0.9% of wild-type activity. 1 Publication1
Mutagenesisi248F → A: 0.9% of wild-type activity. 1 Publication1
Mutagenesisi248F → V: 3.3% of wild-type activity. 1 Publication1
Mutagenesisi249S → G: 93% of wild-type activity. 1 Publication1
Mutagenesisi250D → A: 8.3% of wild-type activity. 1 Publication1
Mutagenesisi251S → G: 87% of wild-type activity. 1 Publication1
Mutagenesisi252N → A: 31% of wild-type activity. 1 Publication1
Mutagenesisi252N → H: 0.4% of wild-type activity. 1 Publication1
Mutagenesisi252N → I: 0.3% of wild-type activity. 1 Publication1
Mutagenesisi252N → S: 37% of wild-type activity. 1 Publication1
Mutagenesisi253H → A: 48% of wild-type activity. 1 Publication1
Mutagenesisi253H → Q: 32% of wild-type activity. 1 Publication1
Mutagenesisi254F → A: 1.2% of wild-type activity. 1 Publication1
Mutagenesisi255S → A: 80% of wild-type activity. 1 Publication1
Mutagenesisi256T → A: 61% of wild-type activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001945631 – 278HTH-type transcriptional activator RhaSAdd BLAST278

Proteomic databases

PaxDbiP09377
PRIDEiP09377

Expressioni

Inductioni

Induced by RhaR in response to L-rhamnose. Binding of the cAMP receptor protein (CRP) is required for full expression.1 Publication

Interactioni

Subunit structurei

Binds DNA as a dimer.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei241Interaction with sigma-701
Sitei250Interaction with sigma-701

Protein-protein interaction databases

BioGridi4260998, 4 interactors
DIPiDIP-10694N
IntActiP09377, 24 interactors
STRINGi316385.ECDH10B_4095

Structurei

3D structure databases

ProteinModelPortaliP09377
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107S4Z Bacteria
ENOG410XT8S LUCA
HOGENOMiHOG000290513
InParanoidiP09377
KOiK02855
OMAiGHYPSHW
PhylomeDBiP09377

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_01534 HTH_type_RhaS, 1 hit
InterProiView protein in InterPro
IPR003313 AraC-bd
IPR009057 Homeobox-like_sf
IPR037923 HTH-like
IPR018060 HTH_AraC
IPR018062 HTH_AraC-typ_CS
IPR014710 RmlC-like_jellyroll
IPR020449 Tscrpt_reg_HTH_AraC-type
IPR023609 Tscrpt_reg_HTH_RhaS
PfamiView protein in Pfam
PF02311 AraC_binding, 1 hit
PF12833 HTH_18, 1 hit
PRINTSiPR00032 HTHARAC
SMARTiView protein in SMART
SM00342 HTH_ARAC, 1 hit
SUPFAMiSSF46689 SSF46689, 2 hits
SSF51215 SSF51215, 1 hit
PROSITEiView protein in PROSITE
PS00041 HTH_ARAC_FAMILY_1, 1 hit
PS01124 HTH_ARAC_FAMILY_2, 1 hit

Sequencei

Sequence statusi: Complete.

P09377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVLHSVDFF PSGNASVAIE PRLPQADFPE HHHDFHEIVI VEHGTGIHVF
60 70 80 90 100
NGQPYTITGG TVCFVRDHDR HLYEHTDNLC LTNVLYRSPD RFQFLAGLNQ
110 120 130 140 150
LLPQELDGQY PSHWRVNHSV LQQVRQLVAQ MEQQEGENDL PSTASREILF
160 170 180 190 200
MQLLLLLRKS SLQENLENSA SRLNLLLAWL EDHFADEVNW DAVADQFSLS
210 220 230 240 250
LRTLHRQLKQ QTGLTPQRYL NRLRLMKARH LLRHSEASVT DIAYRCGFSD
260 270
SNHFSTLFRR EFNWSPRDIR QGRDGFLQ
Length:278
Mass (Da):32,315
Last modified:July 1, 1989 - v1
Checksum:i64264514484C0D2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06058 Genomic DNA Translation: CAA29452.1
L19201 Genomic DNA Translation: AAB03038.1
U00096 Genomic DNA Translation: AAC76887.1
AP009048 Genomic DNA Translation: BAE77404.1
PIRiS40849
RefSeqiNP_418341.1, NC_000913.3
WP_000217137.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76887; AAC76887; b3905
BAE77404; BAE77404; BAE77404
GeneIDi948397
KEGGiecj:JW3876
eco:b3905
PATRICifig|1411691.4.peg.2800

Similar proteinsi

Entry informationi

Entry nameiRHAS_ECOLI
AccessioniPrimary (citable) accession number: P09377
Secondary accession number(s): Q2M8K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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