Reviewed, UniProtKB/Swiss-Prot P09368 (PUT1_YEAST)

Names and origin

Protein names

Recommended name:
    Proline dehydrogenase, mitochondrial
    EC=1.5.99.8
Alternative name(s):
    Proline oxidase

Gene names

Name:	PUT1
Ordered Locus Names:	YLR142W
ORF Names:	L3170, L9606.2

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts proline to delta-1-pyrroline-5-carboxylate.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
Subcellular location	Mitochondrion matrix.
Miscellaneous	The expression of proline oxidase is controlled by proline and oxygen. Proline oxidase requires a functional electron transport chain aerobiosis for its activity. Present with 184 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the proline oxidase family.

Ontologies

Keywords
Biological process	Proline metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Traceable author statement. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline catabolic process to glutamate Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	proline dehydrogenase activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 476		Proline dehydrogenase, mitochondrial		PRO_0000025806
Experimental info
	Sequence conflict	50	1	A → P in AAA16631. Ref.1
	Sequence conflict	185	1	N → T in AAA16631. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P09368-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 12FEE55F86E1707D	FASTA	476	53,271
	10 20 30 40 50 60 MIASKSSLLV TKSRIPSLCF PLIKRSYVSK TPTHSNTAAN LMVETPAANA NGNSVMAPPN 70 80 90 100 110 120 SINFLQTLPK KELFQLGFIG IATLNSFFLN TIIKLFPYIP IPVIKFFVSS LYCGGENFKE 130 140 150 160 170 180 VIECGKRLQK RGISNMMLSL TIENSEGTKS LSSTPVDQIV KETISSVHNI LLPNIIGQLE 190 200 210 220 230 240 SKPINDIAPG YIALKPSALV DNPHEVLYNF SNPAYKAQRD QLIENCSKIT KEIFELNQSL 250 260 270 280 290 300 LKKYPERKAP FMVSTIDAEK YDLQENGVYE LQRILFQKFN PTSSKLISCV GTWQLYLRDS 310 320 330 340 350 360 GDHILHELKL AQENGYKLGL KLVRGAYIHS EKNRNQIIFG DKTGTDENYD RIITQVVNDL 370 380 390 400 410 420 IINGEDSYFG HLVVASHNYQ SQMLVTNLLK STQDNSYAKS NIVLGQLLGM ADNVTYDLIT 430 440 450 460 470 NHGAKNIIKY VPWGPPLETK DYLLRRLQEN GDAVRSDNGW PLIKAIAKSI PKRVGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proline utilization in Saccharomyces cerevisiae: sequence, regulation, and mitochondrial localization of the PUT1 gene product." Wang S.-S., Brandriss M.C. Mol. Cell. Biol. 7:4431-4440(1987) [PubMed: 3125423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M18107 Unassigned DNA. Translation: AAA16631.1. X91258 Genomic DNA. Translation: CAA62662.1. Z73314 Genomic DNA. Translation: CAA97714.1. U53881 Genomic DNA. Translation: AAB82390.1. AY692906 Genomic DNA. Translation: AAT92925.1.
PIR	S59339.
RefSeq	NP_013243.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P09368.
Genome annotation databases
Ensembl	YLR142W; YLR142W; YLR142W; Saccharomyces cerevisiae. [Genome view]
GeneID	850833.
GenomeReviews	Gene locus YLR142W in contig Y13138_GR.
KEGG	sce:YLR142W.
NMPDR	fig|4932.3.peg.4236.
Organism-specific databases
CYGD	YLR142w.
SGD	S000004132. PUT1.
Phylogenomic databases
HOGENOM	P09368.
OMA	KYVPWGP.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-645.
BRENDA	1.5.99.8. 250.
Gene expression databases
ArrayExpress	P09368.
Genevestigator	P09368.
GermOnline	YLR142W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002872. Proline_DH. IPR015659. Proline_oxidase. [Graphical view]
PANTHER	PTHR13914. Proline_oxidase. 1 hit.
Pfam	PF01619. Pro_dh. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967103.

Entry information

Entry name

PUT1_YEAST

Accession

Primary (citable) accession number: P09368

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)