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Protein

Proline dehydrogenase, mitochondrial

Gene

PUT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts proline to delta-1-pyrroline-5-carboxylate.

Catalytic activityi

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol.

Cofactori

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from L-proline.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Proline dehydrogenase, mitochondrial (PUT1)
  2. Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (PUT2)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

GO - Molecular functioni

  • FAD binding Source: GO_Central
  • proline dehydrogenase activity Source: SGD

GO - Biological processi

  • glutamate biosynthetic process Source: SGD
  • proline catabolic process to glutamate Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Proline metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:YLR142W-MONOMER.
YEAST:YLR142W-MONOMER.
ReactomeiR-SCE-70688. Proline catabolism.
UniPathwayiUPA00261; UER00373.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline dehydrogenase, mitochondrial (EC:1.5.5.2)
Alternative name(s):
Proline oxidase
Gene namesi
Name:PUT1
Ordered Locus Names:YLR142W
ORF Names:L3170, L9606.2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR142W.
SGDiS000004132. PUT1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000025806? – 476Proline dehydrogenase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiP09368.
PRIDEiP09368.

PTM databases

iPTMnetiP09368.

Interactioni

Protein-protein interaction databases

BioGridi31410. 16 interactors.
IntActiP09368. 6 interactors.
MINTiMINT-4495075.

Structurei

3D structure databases

ProteinModelPortaliP09368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the proline oxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000006265.
HOGENOMiHOG000248390.
InParanoidiP09368.
KOiK00318.
OMAiGTWQLYL.
OrthoDBiEOG092C26ET.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR002872. Proline_DH_dom.
IPR015659. Proline_oxidase.
[Graphical view]
PANTHERiPTHR13914. PTHR13914. 1 hit.
PfamiPF01619. Pro_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIASKSSLLV TKSRIPSLCF PLIKRSYVSK TPTHSNTAAN LMVETPAANA
60 70 80 90 100
NGNSVMAPPN SINFLQTLPK KELFQLGFIG IATLNSFFLN TIIKLFPYIP
110 120 130 140 150
IPVIKFFVSS LYCGGENFKE VIECGKRLQK RGISNMMLSL TIENSEGTKS
160 170 180 190 200
LSSTPVDQIV KETISSVHNI LLPNIIGQLE SKPINDIAPG YIALKPSALV
210 220 230 240 250
DNPHEVLYNF SNPAYKAQRD QLIENCSKIT KEIFELNQSL LKKYPERKAP
260 270 280 290 300
FMVSTIDAEK YDLQENGVYE LQRILFQKFN PTSSKLISCV GTWQLYLRDS
310 320 330 340 350
GDHILHELKL AQENGYKLGL KLVRGAYIHS EKNRNQIIFG DKTGTDENYD
360 370 380 390 400
RIITQVVNDL IINGEDSYFG HLVVASHNYQ SQMLVTNLLK STQDNSYAKS
410 420 430 440 450
NIVLGQLLGM ADNVTYDLIT NHGAKNIIKY VPWGPPLETK DYLLRRLQEN
460 470
GDAVRSDNGW PLIKAIAKSI PKRVGL
Length:476
Mass (Da):53,271
Last modified:February 1, 1996 - v2
Checksum:i12FEE55F86E1707D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50A → P in AAA16631 (PubMed:3125423).Curated1
Sequence conflicti185N → T in AAA16631 (PubMed:3125423).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18107 Unassigned DNA. Translation: AAA16631.1.
X91258 Genomic DNA. Translation: CAA62662.1.
Z73314 Genomic DNA. Translation: CAA97714.1.
U53881 Genomic DNA. Translation: AAB82390.1.
AY692906 Genomic DNA. Translation: AAT92925.1.
BK006945 Genomic DNA. Translation: DAA09452.1.
PIRiS59339.
RefSeqiNP_013243.1. NM_001182029.1.

Genome annotation databases

EnsemblFungiiYLR142W; YLR142W; YLR142W.
GeneIDi850833.
KEGGisce:YLR142W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18107 Unassigned DNA. Translation: AAA16631.1.
X91258 Genomic DNA. Translation: CAA62662.1.
Z73314 Genomic DNA. Translation: CAA97714.1.
U53881 Genomic DNA. Translation: AAB82390.1.
AY692906 Genomic DNA. Translation: AAT92925.1.
BK006945 Genomic DNA. Translation: DAA09452.1.
PIRiS59339.
RefSeqiNP_013243.1. NM_001182029.1.

3D structure databases

ProteinModelPortaliP09368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31410. 16 interactors.
IntActiP09368. 6 interactors.
MINTiMINT-4495075.

PTM databases

iPTMnetiP09368.

Proteomic databases

MaxQBiP09368.
PRIDEiP09368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR142W; YLR142W; YLR142W.
GeneIDi850833.
KEGGisce:YLR142W.

Organism-specific databases

EuPathDBiFungiDB:YLR142W.
SGDiS000004132. PUT1.

Phylogenomic databases

GeneTreeiENSGT00390000006265.
HOGENOMiHOG000248390.
InParanoidiP09368.
KOiK00318.
OMAiGTWQLYL.
OrthoDBiEOG092C26ET.

Enzyme and pathway databases

UniPathwayiUPA00261; UER00373.
BioCyciMetaCyc:YLR142W-MONOMER.
YEAST:YLR142W-MONOMER.
ReactomeiR-SCE-70688. Proline catabolism.

Miscellaneous databases

PROiP09368.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR002872. Proline_DH_dom.
IPR015659. Proline_oxidase.
[Graphical view]
PANTHERiPTHR13914. PTHR13914. 1 hit.
PfamiPF01619. Pro_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUT1_YEAST
AccessioniPrimary (citable) accession number: P09368
Secondary accession number(s): D6VYD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The expression of proline oxidase is controlled by proline and oxygen.
Proline oxidase requires a functional electron transport chain aerobiosis for its activity.
Present with 184 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.