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P09366

- NSP2_ROTP5

UniProt

P09366 - NSP2_ROTP5

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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Magnesium is required for NTPase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531MagnesiumSequence Analysis
Metal bindingi171 – 1711MagnesiumSequence Analysis
Active sitei225 – 2251For NTPase activityBy similarity

GO - Molecular functioni

  1. hydrolase activity, acting on acid anhydrides Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Taxonomic identifieri10915 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Host cytoplasm Curated
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Non-structural protein 2PRO_0000149549Add
BLAST

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP09366.
SMRiP09366. Positions 1-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 24137RNA-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

P09366-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAELACFCYP HLEKDSYKFI PFNSLAIKCM LTAKVDKKDQ DKFYNSIVYG
60 70 80 90 100
IAPPPQFKKR YNTNDNSRGM NFETSMFNKV AILICEALNS IKVTQSDVAN
110 120 130 140 150
VLSRVVSVRH LENLVLRKEN HQDVLFHSKE LLLKAVLIAI GQSKEIETTA
160 170 180 190 200
TAEGGEIVFQ NAAFTMWKLT YLDHKLMPIL DQNFIEYKIT LNEDKPISDA
210 220 230 240 250
CVKELVAELR WQYNRFAVIT HGKGHYRVVK YSSVANHADR VFATYKNNAK
260 270 280 290 300
SGNVTDFNLL DQRIIWQNWY AFTSSMKQGN TLDVCKKLLF QKMKQEKNPF
310
KGLSTDRKMD EVSHVGI
Length:317
Mass (Da):36,456
Last modified:July 1, 1989 - v1
Checksum:i333C3F7D831A7E7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06722 mRNA. Translation: CAA29902.1.
PIRiS01747. WMXR5U.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06722 mRNA. Translation: CAA29902.1 .
PIRi S01747. WMXR5U.

3D structure databases

ProteinModelPortali P09366.
SMRi P09366. Positions 1-313.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProi IPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view ]
Pfami PF02509. Rota_NS35. 1 hit.
[Graphical view ]
SUPFAMi SSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of porcine rotavirus (OSU strain) gene segments 7, 8, and 9."
    Rushlow K., McNab A., Olson K., Maxwell F., Maxwell I., Stiegler G.
    Nucleic Acids Res. 16:367-368(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNSP2_ROTP5
AccessioniPrimary (citable) accession number: P09366
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 26, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3