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P09366 (NSP2_ROTP5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 2

Short name=NSP2
EC=3.6.4.-
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name=NS35
OrganismRotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Taxonomic identifier10915 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostSus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.

Cofactor

Magnesium for NTPase activity By similarity.

Subunit structure

Homooctamer By similarity. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories By similarity.

Subcellular location

Host cytoplasm Potential. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Sequence similarities

Belongs to the rotavirus NSP2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Non-structural protein 2
PRO_0000149549

Regions

Region205 – 24137RNA-binding Potential

Sites

Active site2251For NTPase activity By similarity
Metal binding1531Magnesium Potential
Metal binding1711Magnesium Potential

Sequences

Sequence LengthMass (Da)Tools
P09366 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 333C3F7D831A7E7C

FASTA31736,456
        10         20         30         40         50         60 
MAELACFCYP HLEKDSYKFI PFNSLAIKCM LTAKVDKKDQ DKFYNSIVYG IAPPPQFKKR 

        70         80         90        100        110        120 
YNTNDNSRGM NFETSMFNKV AILICEALNS IKVTQSDVAN VLSRVVSVRH LENLVLRKEN 

       130        140        150        160        170        180 
HQDVLFHSKE LLLKAVLIAI GQSKEIETTA TAEGGEIVFQ NAAFTMWKLT YLDHKLMPIL 

       190        200        210        220        230        240 
DQNFIEYKIT LNEDKPISDA CVKELVAELR WQYNRFAVIT HGKGHYRVVK YSSVANHADR 

       250        260        270        280        290        300 
VFATYKNNAK SGNVTDFNLL DQRIIWQNWY AFTSSMKQGN TLDVCKKLLF QKMKQEKNPF 

       310 
KGLSTDRKMD EVSHVGI 

« Hide

References

[1]"Nucleotide sequence of porcine rotavirus (OSU strain) gene segments 7, 8, and 9."
Rushlow K., McNab A., Olson K., Maxwell F., Maxwell I., Stiegler G.
Nucleic Acids Res. 16:367-368(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06722 mRNA. Translation: CAA29902.1.
PIRWMXR5U. S01747.

3D structure databases

ProteinModelPortalP09366.
SMRP09366. Positions 1-313.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP2_ROTP5
AccessionPrimary (citable) accession number: P09366
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families