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P09358 (MTD22_STREE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase DpnIIB

Short name=M.DpnIIB
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase DpnIIB
M.DpnII 2
Gene names
Name:dpnA
OrganismStreptococcus pneumoniae
Taxonomic identifier1313 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for establishment of a previously unmethylated plasmid transformed into the cell as single-stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and protects the DNA from cleavage by the DpnII endonuclease. Ref.3

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Homodimer.

Miscellaneous

The DpnII restriction system has two different methylases.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: P09358-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P09358-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: Missing.
Note: Produced in response to the induction of competence for genetic transformation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 268268Modification methylase DpnIIB
PRO_0000030349

Natural variations

Alternative sequence1 – 1212Missing in isoform 2.
VSP_018869

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: DD039104E3A915FC

FASTA26830,922
        10         20         30         40         50         60 
MKNNEYKYGG VLMTKPYYNK NKMILVHSDT FKFLSKMKPE SMDMIFADPP YFLSNGGISN 

        70         80         90        100        110        120 
SGGQVVSVDK GDWDKISSFE EKHEFNRKWI RLAKEVLKPN GTVWISGSLH NIYSVGMALE 

       130        140        150        160        170        180 
QEGFKILNNI TWQKTNPAPN LSCRYFTHST ETILWARKND KKARHYYNYD LMKELNDGKQ 

       190        200        210        220        230        240 
MKDVWTGSLT KKVEKWAGKH PTQKPEYLLE RIILASTKEG DYILDPFVGS GTTGVVAKRL 

       250        260 
GRRFIGIDAE KEYLKIARKR LEAENETN 

« Hide

Isoform 2 [UniParc].

Checksum: 6CEC667BE4907954
Show »

FASTA25629,525

References

[1]"Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
[2]"Regulation of competence for genetic transformation in Streptococcus pneumoniae: expression of dpnA, a late competence gene encoding a DNA methyltransferase of the DpnII restriction system."
Lacks S.A., Ayalew S., de la Campa A.G., Greenberg B.
Mol. Microbiol. 35:1089-1098(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), SEQUENCE REVISION TO N-TERMINUS, ALTERNATIVE INITIATION.
[3]"Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease."
de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A.
J. Mol. Biol. 196:457-469(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-13 (ISOFORM 1), FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14339 Genomic DNA. Translation: AAA88581.2.
PIRA24372.

3D structure databases

ProteinModelPortalP09358.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3637. M2.DpnII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTD22_STREE
AccessionPrimary (citable) accession number: P09358
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries