P09358 (MTD22_STREE) Reviewed, UniProtKB/Swiss-Prot
Last modified October 16, 2013. Version 92. History...
Names and origin
|Protein names||Recommended name:|
Modification methylase DpnIIB
Adenine-specific methyltransferase DpnIIB
|Taxonomic identifier||1313 [NCBI]|
|Taxonomic lineage||Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus|
|Sequence length||268 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Essential for establishment of a previously unmethylated plasmid transformed into the cell as single-stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and protects the DNA from cleavage by the DpnII endonuclease. Ref.3
S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
The DpnII restriction system has two different methylases.
Belongs to the N(4)/N(6)-methyltransferase family.
|Biological process||Restriction system|
|Coding sequence diversity||Alternative initiation|
|Technical term||Direct protein sequencing|
|Gene Ontology (GO)|
|Biological_process||DNA methylation on adenine|
Inferred from electronic annotation. Source: GOCDNA restriction-modification system
Inferred from electronic annotation. Source: UniProtKB-KW
Inferred from electronic annotation. Source: InterProN-methyltransferase activity
Inferred from electronic annotation. Source: InterProsite-specific DNA-methyltransferase (adenine-specific) activity
Inferred from electronic annotation. Source: UniProtKB-EC
|Complete GO annotation...|
|This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]|
|Isoform 1 (identifier: P09358-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform 2 (identifier: P09358-2) |
The sequence of this isoform differs from the canonical sequence as follows:
|Note: Produced in response to the induction of competence for genetic transformation.|
Sequence annotation (Features)
|||"Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."|
Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
|||"Regulation of competence for genetic transformation in Streptococcus pneumoniae: expression of dpnA, a late competence gene encoding a DNA methyltransferase of the DpnII restriction system."|
Lacks S.A., Ayalew S., de la Campa A.G., Greenberg B.
Mol. Microbiol. 35:1089-1098(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), SEQUENCE REVISION TO N-TERMINUS, ALTERNATIVE INITIATION.
|||"Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease."|
de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A.
J. Mol. Biol. 196:457-469(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-13 (ISOFORM 1), FUNCTION.
|M14339 Genomic DNA. Translation: AAA88581.2.|
3D structure databases
Protein family/group databases
|REBASE||3637. M2.DpnII. |
Protocols and materials databases
Family and domain databases
|InterPro||IPR002941. DNA_methylase_N4/N6. |
|Pfam||PF01555. N6_N4_Mtase. 1 hit. |
|PRINTS||PR00508. S21N4MTFRASE. |
|PROSITE||PS00092. N6_MTASE. 1 hit. |
|Accession||Primary (citable) accession number: P09358|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Prokaryotic Protein Annotation Program|