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P09358

- MTD22_STREE

UniProt

P09358 - MTD22_STREE

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Protein

Modification methylase DpnIIB

Gene

dpnA

Organism
Streptococcus pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Essential for establishment of a previously unmethylated plasmid transformed into the cell as single-stranded DNA. Recognizes the single- or double-stranded sequence GATC, causes specific methylation on A-2 on one or both strands (respectively), and protects the DNA from cleavage by the DpnII endonuclease.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3637. M2.DpnII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase DpnIIB (EC:2.1.1.72)
Short name:
M.DpnIIB
Alternative name(s):
Adenine-specific methyltransferase DpnIIB
M.DpnII 2
Gene namesi
Name:dpnA
OrganismiStreptococcus pneumoniae
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Modification methylase DpnIIBPRO_0000030349Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP09358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: P09358-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKNNEYKYGG VLMTKPYYNK NKMILVHSDT FKFLSKMKPE SMDMIFADPP
60 70 80 90 100
YFLSNGGISN SGGQVVSVDK GDWDKISSFE EKHEFNRKWI RLAKEVLKPN
110 120 130 140 150
GTVWISGSLH NIYSVGMALE QEGFKILNNI TWQKTNPAPN LSCRYFTHST
160 170 180 190 200
ETILWARKND KKARHYYNYD LMKELNDGKQ MKDVWTGSLT KKVEKWAGKH
210 220 230 240 250
PTQKPEYLLE RIILASTKEG DYILDPFVGS GTTGVVAKRL GRRFIGIDAE
260
KEYLKIARKR LEAENETN
Length:268
Mass (Da):30,922
Last modified:July 1, 1989 - v1
Checksum:iDD039104E3A915FC
GO
Isoform 2 (identifier: P09358-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: Missing.

Note: Produced in response to the induction of competence for genetic transformation.

Show »
Length:256
Mass (Da):29,525
Checksum:i6CEC667BE4907954
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1212Missing in isoform 2. CuratedVSP_018869Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14339 Genomic DNA. Translation: AAA88581.2.
PIRiA24372.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14339 Genomic DNA. Translation: AAA88581.2 .
PIRi A24372.

3D structure databases

ProteinModelPortali P09358.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3637. M2.DpnII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00508. S21N4MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
    Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
    Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
  2. "Regulation of competence for genetic transformation in Streptococcus pneumoniae: expression of dpnA, a late competence gene encoding a DNA methyltransferase of the DpnII restriction system."
    Lacks S.A., Ayalew S., de la Campa A.G., Greenberg B.
    Mol. Microbiol. 35:1089-1098(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), SEQUENCE REVISION TO N-TERMINUS, ALTERNATIVE INITIATION.
  3. "Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease."
    de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A.
    J. Mol. Biol. 196:457-469(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-13 (ISOFORM 1), FUNCTION.

Entry informationi

Entry nameiMTD22_STREE
AccessioniPrimary (citable) accession number: P09358
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 1, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The DpnII restriction system has two different methylases.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3