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P09357

- T2D2_STREE

UniProt

P09357 - T2D2_STREE

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Protein

Type-2 restriction enzyme DpnII

Gene

dpnB

Organism
Streptococcus pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi777. DpnII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme DpnII (EC:3.1.21.4)
Short name:
R.DpnII
Alternative name(s):
Endonuclease DpnII
Type II restriction enzyme DpnII
Gene namesi
Name:dpnB
OrganismiStreptococcus pneumoniae
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Type-2 restriction enzyme DpnIIPRO_0000077302Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view]
PfamiPF04556. DpnII. 1 hit.
[Graphical view]
PIRSFiPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.

Sequencei

Sequence statusi: Complete.

P09357-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQTRNFDEW LSTMTDTVAD WTYYTDFPKV YKNVSSIKVA LNIMNSLIGS
60 70 80 90 100
KNIQEDFLDL YQNYPEILKV VPLLIAKRLR DTIIVKDPIK DFYFDFSKRN
110 120 130 140 150
YSIEEYTMFL EKSGIFDLLQ NHLVSNLVDY VTGVEVGMDT NGRKNRTGDA
160 170 180 190 200
MENIVQSYLE AEGYILGENL FKEIEQNEIE EIFSVDLSAI TNDGNTVKRF
210 220 230 240 250
DFVIKNEQVL YLIEVNFYSG SGSKLNETAR SYKMIAEETK AIPNVEFMWI
260 270 280
TDGQGWYKAK NNLRETFDIL PFLYNINDLE HNILKNLK
Length:288
Mass (Da):33,585
Last modified:July 1, 1989 - v1
Checksum:iF7346EEA6940EB33
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14339 Genomic DNA. Translation: AAA88582.1.
PIRiB24372.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14339 Genomic DNA. Translation: AAA88582.1 .
PIRi B24372.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 777. DpnII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view ]
Pfami PF04556. DpnII. 1 hit.
[Graphical view ]
PIRSFi PIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
    Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
    Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease."
    de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A.
    J. Mol. Biol. 196:457-469(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-11.

Entry informationi

Entry nameiT2D2_STREE
AccessioniPrimary (citable) accession number: P09357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 1, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3