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P09357 (T2D2_STREE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme DpnII

Short name=R.DpnII
EC=3.1.21.4
Alternative name(s):
Endonuclease DpnII
Type II restriction enzyme DpnII
Gene names
Name:dpnB
OrganismStreptococcus pneumoniae
Taxonomic identifier1313 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the type II restriction enzyme DpnII family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Type-2 restriction enzyme DpnII
PRO_0000077302

Sequences

Sequence LengthMass (Da)Tools
P09357 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: F7346EEA6940EB33

FASTA28833,585
        10         20         30         40         50         60 
MKQTRNFDEW LSTMTDTVAD WTYYTDFPKV YKNVSSIKVA LNIMNSLIGS KNIQEDFLDL 

        70         80         90        100        110        120 
YQNYPEILKV VPLLIAKRLR DTIIVKDPIK DFYFDFSKRN YSIEEYTMFL EKSGIFDLLQ 

       130        140        150        160        170        180 
NHLVSNLVDY VTGVEVGMDT NGRKNRTGDA MENIVQSYLE AEGYILGENL FKEIEQNEIE 

       190        200        210        220        230        240 
EIFSVDLSAI TNDGNTVKRF DFVIKNEQVL YLIEVNFYSG SGSKLNETAR SYKMIAEETK 

       250        260        270        280 
AIPNVEFMWI TDGQGWYKAK NNLRETFDIL PFLYNINDLE HNILKNLK 

« Hide

References

[1]"Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease."
de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A.
J. Mol. Biol. 196:457-469(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-11.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14339 Genomic DNA. Translation: AAA88582.1.
PIRB24372.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE777. DpnII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR021191. Restrct_endonuc_II_DpmII.
IPR007637. Restrct_endonuc_II_DpmII-like.
[Graphical view]
PfamPF04556. DpnII. 1 hit.
[Graphical view]
PIRSFPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2D2_STREE
AccessionPrimary (citable) accession number: P09357
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries