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P09357

- T2D2_STREE

UniProt

P09357 - T2D2_STREE

Protein

Type-2 restriction enzyme DpnII

Gene

dpnB

Organism
Streptococcus pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    • Comment

    Functioni

    Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi777. DpnII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme DpnII (EC:3.1.21.4)
    Short name:
    R.DpnII
    Alternative name(s):
    Endonuclease DpnII
    Type II restriction enzyme DpnII
    Gene namesi
    Name:dpnB
    OrganismiStreptococcus pneumoniae
    Taxonomic identifieri1313 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Type-2 restriction enzyme DpnIIPRO_0000077302Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR021191. Restrct_endonuc_II_DpmII.
    IPR007637. Restrct_endonuc_II_DpmII-like.
    [Graphical view]
    PfamiPF04556. DpnII. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P09357-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQTRNFDEW LSTMTDTVAD WTYYTDFPKV YKNVSSIKVA LNIMNSLIGS    50
    KNIQEDFLDL YQNYPEILKV VPLLIAKRLR DTIIVKDPIK DFYFDFSKRN 100
    YSIEEYTMFL EKSGIFDLLQ NHLVSNLVDY VTGVEVGMDT NGRKNRTGDA 150
    MENIVQSYLE AEGYILGENL FKEIEQNEIE EIFSVDLSAI TNDGNTVKRF 200
    DFVIKNEQVL YLIEVNFYSG SGSKLNETAR SYKMIAEETK AIPNVEFMWI 250
    TDGQGWYKAK NNLRETFDIL PFLYNINDLE HNILKNLK 288
    Length:288
    Mass (Da):33,585
    Last modified:July 1, 1989 - v1
    Checksum:iF7346EEA6940EB33
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14339 Genomic DNA. Translation: AAA88582.1.
    PIRiB24372.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14339 Genomic DNA. Translation: AAA88582.1 .
    PIRi B24372.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 777. DpnII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR021191. Restrct_endonuc_II_DpmII.
    IPR007637. Restrct_endonuc_II_DpmII-like.
    [Graphical view ]
    Pfami PF04556. DpnII. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism."
      Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.
      Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease."
      de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A.
      J. Mol. Biol. 196:457-469(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-11.

    Entry informationi

    Entry nameiT2D2_STREE
    AccessioniPrimary (citable) accession number: P09357
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3