P09357 (T2D2_STREE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme DpnII
Short name=R.DpnII
EC=3.1.21.4

Alternative name(s):
Endonuclease DpnII
Type II restriction enzyme DpnII

Gene names

Name:

dpnB

Organism

Streptococcus pneumoniae

Taxonomic identifier

1313 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes the double-stranded unmethylated sequence GATC and cleaves before G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the type II restriction enzyme DpnII family.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Type-2 restriction enzyme DpnII		PRO_0000077302

Sequences

	Sequence		Length	Mass (Da)
	P09357 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: F7346EEA6940EB33	FASTA	288	33,585
	10 20 30 40 50 60 MKQTRNFDEW LSTMTDTVAD WTYYTDFPKV YKNVSSIKVA LNIMNSLIGS KNIQEDFLDL 70 80 90 100 110 120 YQNYPEILKV VPLLIAKRLR DTIIVKDPIK DFYFDFSKRN YSIEEYTMFL EKSGIFDLLQ 130 140 150 160 170 180 NHLVSNLVDY VTGVEVGMDT NGRKNRTGDA MENIVQSYLE AEGYILGENL FKEIEQNEIE 190 200 210 220 230 240 EIFSVDLSAI TNDGNTVKRF DFVIKNEQVL YLIEVNFYSG SGSKLNETAR SYKMIAEETK 250 260 270 280 AIPNVEFMWI TDGQGWYKAK NNLRETFDIL PFLYNINDLE HNILKNLK

References

[1]	"Genetic basis of the complementary DpnI and DpnII restriction systems of S. pneumoniae: an intercellular cassette mechanism." Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B. Cell 46:993-1000(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Proteins encoded by the DpnII restriction gene cassette. Two methylases and an endonuclease." de la Campa A.G., Purushottam K., Springhorn S.S., Lacks S.A. J. Mol. Biol. 196:457-469(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-11.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14339 Genomic DNA. Translation: AAA88582.1.
PIR	B24372.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	777. DpnII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR021191. Restrct_endonuc_II_DpmII. IPR007637. Restrct_endonuc_II_DpmII-like. [Graphical view]
Pfam	PF04556. DpnII. 1 hit. [Graphical view]
PIRSF	PIRSF016080. Restrict_endonuc_II_DpmII. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T2D2_STREE

Accession

Primary (citable) accession number: P09357

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program