P09353 (SR3A_PHYPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Spherulin-3A Short name=S3A
Organism	Physarum polycephalum (Slime mold)
Taxonomic identifier	5791 [NCBI]
Taxonomic lineage	Eukaryota › Amoebozoa › Mycetozoa › Myxogastria › Myxogastromycetidae › Physariida › Physaraceae › Physarum

Protein attributes

Sequence length	103 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Structural protein.
Subcellular location	Cytoplasm.
Developmental stage	Major encystment-specific protein.
Sequence similarities	Belongs to the beta/gamma-crystallin family. Contains 2 beta/gamma crystallin 'Greek key' domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 103

103

Spherulin-3A

PRO_0000057608

Regions

Domain

14 – 55

42

Beta/gamma crystallin 'Greek key' 1

Domain

57 – 99

43

Beta/gamma crystallin 'Greek key' 2

Region

1 – 13

13

N-terminal arm

Secondary structure

1

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103

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Beta strand

16 – 21

6

Helix

22 – 24

3

Beta strand

25 – 31

7

Beta strand

33 – 36

4

Helix

38 – 40

3

Turn

42 – 47

6

Beta strand

50 – 53

4

Beta strand

57 – 64

8

Helix

65 – 67

3

Beta strand

69 – 74

6

Beta strand

78 – 81

4

Helix

83 – 86

4

Beta strand

94 – 99

6

Sequences

	Sequence		Length	Mass (Da)
	P09353 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 4D92B1988B965E6D	FASTA	103	11,285
	10 20 30 40 50 60 MSVCKGVSGN PAKGEVFLYK HVNFQGDSWK VTGNVYDFRS VSGLNDVVSS VKVGPNTKAF 70 80 90 100 IFKDDRFNGN FIRLEESSQV TDLTTRNLND AISSIIVATF ESA

References

[1]	"Gene families encode the major encystment-specific proteins of Physarum polycephalum plasmodia." Bernier F., Lemieux G., Pallotta D. Gene 59:265-277(1987) [PubMed: 2830170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Evolution of a protein superfamily: relationships between vertebrate lens crystallins and microorganism dormancy proteins." Wistow G. J. Mol. Evol. 30:140-145(1990) [PubMed: 2107329] [Abstract] Cited for: SIMILARITY TO BETA/GAMMA CRYSTALLINS.
[3]	"Ca2+-loaded spherulin 3a from Physarum polycephalum adopts the prototype gamma-crystallin fold in aqueous solution." Rosinke B., Renner C., Mayr E.-M., Jaenicke R., Holak T.A. J. Mol. Biol. 271:645-655(1997) [PubMed: 9281431] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M18431 mRNA. Translation: AAA29981.1.

PIR

D29624.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AG4	NMR	-	A	1-103	[»]
1HDF	X-ray	2.35	A/B	2-103	[»]

ProteinModelPortal

P09353.

SMR

P09353. Positions 1-103.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001064. Beta/gamma_crystallin.
IPR011024. G_crystallin-rel.
[Graphical view]

Gene3D

G3DSA:2.60.20.10. Crystallin. 1 hit.

Pfam

PF00030. Crystall. 1 hit.
[Graphical view]

SMART

SM00247. XTALbg. 1 hit.
[Graphical view]

SUPFAM

SSF49695. G_crystallin_SF. 1 hit.

PROSITE

PS50915. CRYSTALLIN_BETA_GAMMA. 2 hits.
[Graphical view]

ProtoNet

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Entry information

Entry name

SR3A_PHYPO

Accession

Primary (citable) accession number: P09353

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)