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Protein

Motility protein A

Gene

motA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

MotA and MotB comprise the stator element of the flagellar motor complex. Required for rotation of the flagellar motor. Probable transmembrane proton channel. Overexpression of MotA, with or without MotB, restores motility in a yhjH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication

GO - Molecular functioni

GO - Biological processi

  • bacterial-type flagellum-dependent cell motility Source: EcoliWiki
  • chemotaxis Source: UniProtKB-KW
  • proton transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Chemotaxis, Flagellar rotation, Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN.
ECOL316407:JW1879-MONOMER.

Protein family/group databases

TCDBi1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Motility protein A
Alternative name(s):
Chemotaxis protein MotA
Gene namesi
Name:motA
Synonyms:flaJ
Ordered Locus Names:b1890, JW1879
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10601. motA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2120HelicalSequence analysisAdd
BLAST
Topological domaini22 – 3312PeriplasmicSequence analysisAdd
BLAST
Transmembranei34 – 5118HelicalSequence analysisAdd
BLAST
Topological domaini52 – 170119CytoplasmicSequence analysisAdd
BLAST
Transmembranei171 – 19121HelicalSequence analysisAdd
BLAST
Topological domaini192 – 2009PeriplasmicSequence analysis
Transmembranei201 – 22222HelicalSequence analysisAdd
BLAST
Topological domaini223 – 29573CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi93 – 931G → E: Suppresses yhjH disruption motility mutant, swimming velocity is wild-type. 1 Publication
Mutagenesisi93 – 931G → R: Suppresses yhjH disruption motility mutant, swimming velocity is between disruption and wild-type. 1 Publication
Mutagenesisi93 – 931G → V: Suppresses yhjH disruption motility mutant, swimming velocity is between disruption and wild-type. In the wt background velocity is reduced. 1 Publication
Mutagenesisi96 – 961S → L: Suppresses yhjH disruption motility mutant, swimming velocity is between disruption and wild-type. In the wt background velocity is reduced. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 295295Motility protein APRO_0000189572Add
BLAST

Proteomic databases

PaxDbiP09348.

Interactioni

Subunit structurei

Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels. Interacts with FliG. Interacts with flagellar brake protein YcgR in the flagellar basal bodies (PubMed:20303158). In another study (PubMed:20346719) YcgR was not seen to interact with MotA, but instead with FliM and FliG, also in the flagellar basal body.2 Publications

Protein-protein interaction databases

BioGridi4262245. 486 interactions.
DIPiDIP-10244N.
IntActiP09348. 2 interactions.
MINTiMINT-1250843.
STRINGi511145.b1890.

Structurei

3D structure databases

ProteinModelPortaliP09348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MotA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107RD7. Bacteria.
COG1291. LUCA.
HOGENOMiHOG000254701.
InParanoidiP09348.
KOiK02556.
OMAiAFICDYL.
OrthoDBiEOG651SSP.
PhylomeDBiP09348.

Family and domain databases

InterProiIPR000540. Flag_MotA_CS.
IPR022522. Flagellar_motor_stator_MotA.
IPR002898. MotA_ExbB_proton_chnl.
[Graphical view]
PANTHERiPTHR30433:SF4. PTHR30433:SF4. 1 hit.
PfamiPF01618. MotA_ExbB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03818. MotA1. 1 hit.
PROSITEiPS01307. MOTA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLILLGYLVV LGTVFGGYLM TGGSLGALYQ PAELVIIAGA GIGSFIVGNN
60 70 80 90 100
GKAIKGTLKA LPLLFRRSKY TKAMYMDLLA LLYRLMAKSR QMGMFSLERD
110 120 130 140 150
IENPRESEIF ASYPRILADS VMLDFIVDYL RLIISGHMNT FEIEALMDEE
160 170 180 190 200
IETHESEAEV PANSLALVGD SLPAFGIVAA VMGVVHALGS ADRPAAELGA
210 220 230 240 250
LIAHAMVGTF LGILLAYGFI SPLATVLRQK SAETSKMMQC VKVTLLSNLN
260 270 280 290
GYAPPIAVEF GRKTLYSSER PSFIELEEHV RAVKNPQQQT TTEEA
Length:295
Mass (Da):32,011
Last modified:July 1, 1989 - v1
Checksum:i355DC13EF63DD7D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01652 Genomic DNA. Translation: AAA24177.1.
U00096 Genomic DNA. Translation: AAC74960.1.
AP009048 Genomic DNA. Translation: BAA15711.1.
PIRiA30279. QRECMA.
RefSeqiNP_416404.1. NC_000913.3.
WP_000906340.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74960; AAC74960; b1890.
BAA15711; BAA15711; BAA15711.
GeneIDi947564.
KEGGiecj:JW1879.
eco:b1890.
PATRICi32119107. VBIEscCol129921_1971.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01652 Genomic DNA. Translation: AAA24177.1.
U00096 Genomic DNA. Translation: AAC74960.1.
AP009048 Genomic DNA. Translation: BAA15711.1.
PIRiA30279. QRECMA.
RefSeqiNP_416404.1. NC_000913.3.
WP_000906340.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP09348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262245. 486 interactions.
DIPiDIP-10244N.
IntActiP09348. 2 interactions.
MINTiMINT-1250843.
STRINGi511145.b1890.

Protein family/group databases

TCDBi1.A.30.1.1. the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily.

Proteomic databases

PaxDbiP09348.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74960; AAC74960; b1890.
BAA15711; BAA15711; BAA15711.
GeneIDi947564.
KEGGiecj:JW1879.
eco:b1890.
PATRICi32119107. VBIEscCol129921_1971.

Organism-specific databases

EchoBASEiEB0596.
EcoGeneiEG10601. motA.

Phylogenomic databases

eggNOGiENOG4107RD7. Bacteria.
COG1291. LUCA.
HOGENOMiHOG000254701.
InParanoidiP09348.
KOiK02556.
OMAiAFICDYL.
OrthoDBiEOG651SSP.
PhylomeDBiP09348.

Enzyme and pathway databases

BioCyciEcoCyc:MOTA-FLAGELLAR-MOTOR-STATOR-PROTEIN.
ECOL316407:JW1879-MONOMER.

Miscellaneous databases

PROiP09348.

Family and domain databases

InterProiIPR000540. Flag_MotA_CS.
IPR022522. Flagellar_motor_stator_MotA.
IPR002898. MotA_ExbB_proton_chnl.
[Graphical view]
PANTHERiPTHR30433:SF4. PTHR30433:SF4. 1 hit.
PfamiPF01618. MotA_ExbB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03818. MotA1. 1 hit.
PROSITEiPS01307. MOTA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene sequence and predicted amino acid sequence of the motA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli."
    Dean G.E., Macnab R.M., Stader J., Matsumura P., Burks C.
    J. Bacteriol. 159:991-999(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane."
    Stader J., Matsumura P., Vacante D., Dean G.E., Macnab R.M.
    J. Bacteriol. 166:244-252(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-295.
  6. "Mutations in the MotA protein of Escherichia coli reveal domains critical for proton conduction."
    Blair D.F., Berg H.C.
    J. Mol. Biol. 221:1433-1442(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, TOPOLOGY.
  7. "Membrane topology of the MotA protein of Escherichia coli."
    Zhou J., Fazzio R.T., Blair D.F.
    J. Mol. Biol. 251:237-242(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS, TOPOLOGY.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  9. "Second messenger-mediated adjustment of bacterial swimming velocity."
    Boehm A., Kaiser M., Li H., Spangler C., Kasper C.A., Ackermann M., Kaever V., Sourjik V., Roth V., Jenal U.
    Cell 141:107-116(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YCGR, MUTAGENESIS OF GLY-93 AND SER-96.
    Strain: K12 / MG1655 / ATCC 47076.
  10. "The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop brake' mechanism."
    Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M.
    Mol. Cell 38:128-139(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / RP3098.
  11. "Flagellar motility in bacteria structure and function of flagellar motor."
    Terashima H., Kojima S., Homma M.
    Int. Rev. Cytol. 270:39-85(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiMOTA_ECOLI
AccessioniPrimary (citable) accession number: P09348
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 20, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.