SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P09345

- HEMA_I59A0

UniProt

P09345 - HEMA_I59A0

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hemagglutinin

Gene
HA
Organism
Influenza A virus (strain A/Chicken/Scotland/1959 H5N1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei342 – 3432Cleavage; by host By similarity

GO - Biological processi

  1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
  2. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  3. fusion of virus membrane with host plasma membrane Source: InterPro
  4. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Clathrin- and caveolin-independent endocytosis of virus by host, Clathrin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin
Cleaved into the following 2 chains:
Gene namesi
Name:HA
OrganismiInfluenza A virus (strain A/Chicken/Scotland/1959 H5N1)
Taxonomic identifieri402527 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
Homo sapiens (Human) [TaxID: 9606]
Panthera pardus (Leopard) (Felis pardus) [TaxID: 9691]
Panthera tigris (Tiger) [TaxID: 9694]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000007768: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host apical cell membrane; Single-pass type I membrane protein
Note: Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 527511Extracellular Reviewed predictionAdd
BLAST
Transmembranei528 – 54821Helical; Reviewed predictionAdd
BLAST
Topological domaini549 – 56416Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 Reviewed predictionAdd
BLAST
Chaini17 – 338322Hemagglutinin HA1 chainPRO_0000038899Add
BLAST
Propeptidei339 – 3424Connecting peptidePRO_0000392211
Chaini343 – 564222Hemagglutinin HA2 chainPRO_0000038900Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi20 ↔ 479Interchain (between HA1 and HA2 chains) By similarity
Glycosylationi26 – 261N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi39 – 391N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi58 ↔ 290 By similarity
Disulfide bondi71 ↔ 83 By similarity
Disulfide bondi106 ↔ 151 By similarity
Glycosylationi181 – 1811N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi294 ↔ 318 By similarity
Glycosylationi302 – 3021N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi486 ↔ 490 By similarity
Glycosylationi496 – 4961N-linked (GlcNAc...); by host Reviewed prediction
Lipidationi553 – 5531S-palmitoyl cysteine; by host By similarity
Lipidationi560 – 5601S-palmitoyl cysteine; by host By similarity
Lipidationi563 – 5631S-palmitoyl cysteine; by host By similarity

Post-translational modificationi

In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells By similarity.
Palmitoylated By similarity.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HA1-HA2.

Structurei

3D structure databases

ProteinModelPortaliP09345.
SMRiP09345. Positions 17-337, 343-516.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view]
PfamiPF00509. Hemagglutinin. 1 hit.
[Graphical view]
PRINTSiPR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09345-1 [UniParc]FASTAAdd to Basket

« Hide

MERIVLLLAI VSLVKSDQIC IGYHANKSTK QVDTIMEKNV TVTHAQDILE    50
RTHNGKLCSL NGVKPLILRD CSVAGWLLGN PMCDEFLNVP EWSYIVEKDN 100
PINSLCYPGD FNDYEELKHL LSSTNHFEKI QIIPRSSWSN HDASSGVSSA 150
CPYIGRSSFF RNVVWLIKKD NAYPTIKRSY NNTNQEDLLI LWGIHHPNDA 200
AEQTKLYQNP TTYVSVGTST LNQRSIPEIA TRPKVNGQSG RMEFFWTILK 250
PNDAINFESN GNFIAPEYAY KIVKKGDSAI MKSGLAYGNC DTKCQTPVGA 300
INSSMPFHNI HPHTIGECPK YVKSDRLVLA TGLRNVPQRK KRGLFGAIAG 350
FIEGGWQGMV DGWYGYHHSN EQGSGYAADK ESTQKAIDGI TNKVNSIIDK 400
MNTQFKAVGK EFNNLERRVE NLNKKMEDGF LDVWTYNVEL LVLMENERTL 450
DFHDSNVKNL YDKVRLQLKD NARELGNGCF EFYHKCDNEC MESVRNGTYD 500
YPQYSEEARL NREEISGVKL ESMGVYQILS IYSTVASSLA LAIMIAGLSF 550
WMCSNGSLQC RICI 564
Length:564
Mass (Da):63,671
Last modified:November 13, 2007 - v2
Checksum:i5C57790D6B76255F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891V → L in CAA30719. 1 Publication
Sequence conflicti89 – 891V → L in CAA30680. 1 Publication
Sequence conflicti93 – 931S → L in CAA30719. 1 Publication
Sequence conflicti93 – 931S → L in CAA30680. 1 Publication
Sequence conflicti119 – 1191H → Y in CAA30719. 1 Publication
Sequence conflicti119 – 1191H → Y in CAA30680. 1 Publication
Sequence conflicti131 – 1311Q → R in CAA30719. 1 Publication
Sequence conflicti131 – 1311Q → R in CAA30680. 1 Publication
Sequence conflicti160 – 1601F → L in CAA30719. 1 Publication
Sequence conflicti160 – 1601F → L in CAA30680. 1 Publication
Sequence conflicti170 – 1723DNA → NNT in CAA30719. 1 Publication
Sequence conflicti170 – 1723DNA → NNT in CAA30680. 1 Publication
Sequence conflicti267 – 2671E → R in CAA30719. 1 Publication
Sequence conflicti267 – 2671E → R in CAA30680. 1 Publication
Sequence conflicti300 – 3001A → E in CAA30719. 1 Publication
Sequence conflicti300 – 3001A → E in CAA30680. 1 Publication
Sequence conflicti404 – 4063QFK → RFE in CAA30719. 1 Publication
Sequence conflicti404 – 4063QFK → RFE in CAA30680. 1 Publication
Sequence conflicti458 – 4581K → N in CAA30719. 1 Publication
Sequence conflicti458 – 4581K → N in CAA30680. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07826 Genomic RNA. Translation: CAA30680.1.
X07869 mRNA. Translation: CAA30719.1.
CY015081 Genomic RNA. Translation: ABI85106.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07826 Genomic RNA. Translation: CAA30680.1 .
X07869 mRNA. Translation: CAA30719.1 .
CY015081 Genomic RNA. Translation: ABI85106.1 .

3D structure databases

ProteinModelPortali P09345.
SMRi P09345. Positions 17-337, 343-516.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view ]
Pfami PF00509. Hemagglutinin. 1 hit.
[Graphical view ]
PRINTSi PR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of a cDNA clone of the hemagglutinin gene of influenza A/Chicken/Scotland/59 (H5N1) virus: comparison with contemporary North American and European strains."
    De B.K., Brownlee G.G., Kendal A.P., Shaw M.W.
    Nucleic Acids Res. 16:4181-4182(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_I59A0
AccessioniPrimary (citable) accession number: P09345
Secondary accession number(s): Q0A2H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 13, 2007
Last modified: February 19, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Major glycoprotein, comprises over 80% of the envelope proteins present in virus particle.
The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.
The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi