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P09345

- HEMA_I59A0

UniProt

P09345 - HEMA_I59A0

Protein

Hemagglutinin

Gene

HA

Organism
Influenza A virus (strain A/Chicken/Scotland/1959 H5N1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei342 – 3432Cleavage; by hostBy similarity

    GO - Biological processi

    1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
    2. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    3. fusion of virus membrane with host plasma membrane Source: InterPro
    4. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin

    Keywords - Biological processi

    Clathrin- and caveolin-independent endocytosis of virus by host, Clathrin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin
    Cleaved into the following 2 chains:
    Gene namesi
    Name:HA
    OrganismiInfluenza A virus (strain A/Chicken/Scotland/1959 H5N1)
    Taxonomic identifieri402527 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]
    Homo sapiens (Human) [TaxID: 9606]
    Panthera pardus (Leopard) (Felis pardus) [TaxID: 9691]
    Panthera tigris (Tiger) [TaxID: 9694]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000007768: Genome

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host apical cell membrane; Single-pass type I membrane protein
    Note: Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 338322Hemagglutinin HA1 chainPRO_0000038899Add
    BLAST
    Propeptidei339 – 3424Connecting peptidePRO_0000392211
    Chaini343 – 564222Hemagglutinin HA2 chainPRO_0000038900Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi20 ↔ 479Interchain (between HA1 and HA2 chains)By similarity
    Glycosylationi26 – 261N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi39 – 391N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi58 ↔ 290By similarity
    Disulfide bondi71 ↔ 83By similarity
    Disulfide bondi106 ↔ 151By similarity
    Glycosylationi181 – 1811N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi294 ↔ 318By similarity
    Glycosylationi302 – 3021N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi486 ↔ 490By similarity
    Glycosylationi496 – 4961N-linked (GlcNAc...); by hostSequence Analysis
    Lipidationi553 – 5531S-palmitoyl cysteine; by hostBy similarity
    Lipidationi560 – 5601S-palmitoyl cysteine; by hostBy similarity
    Lipidationi563 – 5631S-palmitoyl cysteine; by hostBy similarity

    Post-translational modificationi

    In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells By similarity.By similarity
    Palmitoylated.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Interactioni

    Subunit structurei

    Homotrimer of disulfide-linked HA1-HA2.

    Structurei

    3D structure databases

    ProteinModelPortaliP09345.
    SMRiP09345. Positions 17-337, 343-516.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 527511ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini549 – 56416CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei528 – 54821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.10.77.10. 1 hit.
    3.90.20.10. 1 hit.
    3.90.209.20. 1 hit.
    InterProiIPR008980. Capsid_hemagglutn.
    IPR013828. Hemagglutn_HA1_a/b_dom.
    IPR013827. Hemagglutn_HA1_b-rbn_dom.
    IPR000149. Hemagglutn_influenz_A.
    IPR001364. Hemagglutn_influenz_A/B.
    IPR013829. Hemagglutn_stalk.
    [Graphical view]
    PfamiPF00509. Hemagglutinin. 1 hit.
    [Graphical view]
    PRINTSiPR00330. HEMAGGLUTN1.
    PR00329. HEMAGGLUTN12.
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09345-1 [UniParc]FASTAAdd to Basket

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    MERIVLLLAI VSLVKSDQIC IGYHANKSTK QVDTIMEKNV TVTHAQDILE    50
    RTHNGKLCSL NGVKPLILRD CSVAGWLLGN PMCDEFLNVP EWSYIVEKDN 100
    PINSLCYPGD FNDYEELKHL LSSTNHFEKI QIIPRSSWSN HDASSGVSSA 150
    CPYIGRSSFF RNVVWLIKKD NAYPTIKRSY NNTNQEDLLI LWGIHHPNDA 200
    AEQTKLYQNP TTYVSVGTST LNQRSIPEIA TRPKVNGQSG RMEFFWTILK 250
    PNDAINFESN GNFIAPEYAY KIVKKGDSAI MKSGLAYGNC DTKCQTPVGA 300
    INSSMPFHNI HPHTIGECPK YVKSDRLVLA TGLRNVPQRK KRGLFGAIAG 350
    FIEGGWQGMV DGWYGYHHSN EQGSGYAADK ESTQKAIDGI TNKVNSIIDK 400
    MNTQFKAVGK EFNNLERRVE NLNKKMEDGF LDVWTYNVEL LVLMENERTL 450
    DFHDSNVKNL YDKVRLQLKD NARELGNGCF EFYHKCDNEC MESVRNGTYD 500
    YPQYSEEARL NREEISGVKL ESMGVYQILS IYSTVASSLA LAIMIAGLSF 550
    WMCSNGSLQC RICI 564
    Length:564
    Mass (Da):63,671
    Last modified:November 13, 2007 - v2
    Checksum:i5C57790D6B76255F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti89 – 891V → L in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti89 – 891V → L in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti93 – 931S → L in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti93 – 931S → L in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti119 – 1191H → Y in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti119 – 1191H → Y in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti131 – 1311Q → R in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti131 – 1311Q → R in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti160 – 1601F → L in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti160 – 1601F → L in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti170 – 1723DNA → NNT in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti170 – 1723DNA → NNT in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti267 – 2671E → R in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti267 – 2671E → R in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti300 – 3001A → E in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti300 – 3001A → E in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti404 – 4063QFK → RFE in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti404 – 4063QFK → RFE in CAA30680. (PubMed:3375087)Curated
    Sequence conflicti458 – 4581K → N in CAA30719. (PubMed:3375087)Curated
    Sequence conflicti458 – 4581K → N in CAA30680. (PubMed:3375087)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07826 Genomic RNA. Translation: CAA30680.1.
    X07869 mRNA. Translation: CAA30719.1.
    CY015081 Genomic RNA. Translation: ABI85106.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07826 Genomic RNA. Translation: CAA30680.1 .
    X07869 mRNA. Translation: CAA30719.1 .
    CY015081 Genomic RNA. Translation: ABI85106.1 .

    3D structure databases

    ProteinModelPortali P09345.
    SMRi P09345. Positions 17-337, 343-516.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.10.77.10. 1 hit.
    3.90.20.10. 1 hit.
    3.90.209.20. 1 hit.
    InterProi IPR008980. Capsid_hemagglutn.
    IPR013828. Hemagglutn_HA1_a/b_dom.
    IPR013827. Hemagglutn_HA1_b-rbn_dom.
    IPR000149. Hemagglutn_influenz_A.
    IPR001364. Hemagglutn_influenz_A/B.
    IPR013829. Hemagglutn_stalk.
    [Graphical view ]
    Pfami PF00509. Hemagglutinin. 1 hit.
    [Graphical view ]
    PRINTSi PR00330. HEMAGGLUTN1.
    PR00329. HEMAGGLUTN12.
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of a cDNA clone of the hemagglutinin gene of influenza A/Chicken/Scotland/59 (H5N1) virus: comparison with contemporary North American and European strains."
      De B.K., Brownlee G.G., Kendal A.P., Shaw M.W.
      Nucleic Acids Res. 16:4181-4182(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiHEMA_I59A0
    AccessioniPrimary (citable) accession number: P09345
    Secondary accession number(s): Q0A2H6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Major glycoprotein, comprises over 80% of the envelope proteins present in virus particle.
    The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.
    The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3