Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P09341

- GROA_HUMAN

UniProt

P09341 - GROA_HUMAN

Protein

Growth-regulated alpha protein

Gene

CXCL1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.1 Publication

    GO - Molecular functioni

    1. chemokine activity Source: ProtInc
    2. enzyme activator activity Source: ProtInc
    3. receptor binding Source: ProtInc

    GO - Biological processi

    1. actin cytoskeleton organization Source: ProtInc
    2. cell chemotaxis Source: GOC
    3. cell proliferation Source: ProtInc
    4. chemotaxis Source: ProtInc
    5. G-protein coupled receptor signaling pathway Source: ProtInc
    6. immune response Source: InterPro
    7. inflammatory response Source: ProtInc
    8. intracellular signal transduction Source: ProtInc
    9. negative regulation of cell proliferation Source: ProtInc
    10. nervous system development Source: ProtInc
    11. positive regulation of catalytic activity Source: GOC
    12. signal transduction Source: ProtInc

    Keywords - Molecular functioni

    Cytokine, Growth factor

    Keywords - Biological processi

    Inflammatory response

    Enzyme and pathway databases

    ReactomeiREACT_15344. Chemokine receptors bind chemokines.
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Growth-regulated alpha protein
    Alternative name(s):
    C-X-C motif chemokine 1
    GRO-alpha(1-73)
    Melanoma growth stimulatory activity
    Short name:
    MGSA
    Neutrophil-activating protein 3
    Short name:
    NAP-3
    Cleaved into the following 3 chains:
    Gene namesi
    Name:CXCL1
    Synonyms:GRO, GRO1, GROA, MGSA, SCYB1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:4602. CXCL1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. extracellular space Source: ProtInc

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35050.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 34345 PublicationsAdd
    BLAST
    Chaini35 – 10773Growth-regulated alpha proteinPRO_0000005049Add
    BLAST
    Chaini38 – 10770GRO-alpha(4-73)PRO_0000005050Add
    BLAST
    Chaini39 – 10769GRO-alpha(5-73)PRO_0000005051Add
    BLAST
    Chaini40 – 10768GRO-alpha(6-73)PRO_0000005052Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 69
    Disulfide bondi45 ↔ 85

    Post-translational modificationi

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.1 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP09341.
    PaxDbiP09341.
    PRIDEiP09341.

    PTM databases

    PhosphoSiteiP09341.

    Miscellaneous databases

    PMAP-CutDBP09341.

    Expressioni

    Gene expression databases

    ArrayExpressiP09341.
    BgeeiP09341.
    CleanExiHS_CXCL1.
    GenevestigatoriP09341.

    Interactioni

    Protein-protein interaction databases

    BioGridi109176. 3 interactions.
    DIPiDIP-5896N.
    IntActiP09341. 1 interaction.
    STRINGi9606.ENSP00000379110.

    Structurei

    Secondary structure

    1
    107
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi41 – 433
    Beta strandi49 – 513
    Turni54 – 563
    Beta strandi57 – 637
    Beta strandi73 – 786
    Beta strandi83 – 864
    Helixi91 – 10313

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MGSNMR-A/B35-107[»]
    1MSGNMR-A/B35-106[»]
    1MSHNMR-A/B35-106[»]
    1RODNMR-A/B40-62[»]
    A/B89-107[»]
    ProteinModelPortaliP09341.
    SMRiP09341. Positions 35-107.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09341.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG266004.
    HOGENOMiHOG000220915.
    HOVERGENiHBG107789.
    InParanoidiP09341.
    KOiK05505.
    OrthoDBiEOG7NSB57.
    PhylomeDBiP09341.
    TreeFamiTF333433.

    Family and domain databases

    InterProiIPR001089. Chemokine_CXC.
    IPR018048. Chemokine_CXC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view]
    PANTHERiPTHR10179. PTHR10179. 1 hit.
    PfamiPF00048. IL8. 1 hit.
    [Graphical view]
    PRINTSiPR00437. SMALLCYTKCXC.
    SMARTiSM00199. SCY. 1 hit.
    [Graphical view]
    SUPFAMiSSF54117. SSF54117. 1 hit.
    PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09341-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARAALSAAP SNPRLLRVAL LLLLLVAAGR RAAGASVATE LRCQCLQTLQ    50
    GIHPKNIQSV NVKSPGPHCA QTEVIATLKN GRKACLNPAS PIVKKIIEKM 100
    LNSDKSN 107
    Length:107
    Mass (Da):11,301
    Last modified:July 1, 1989 - v1
    Checksum:i17048A6B4D765CA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03561 mRNA. Translation: AAA35933.1.
    X12510 mRNA. Translation: CAA31027.1.
    X54489 Genomic DNA. Translation: CAA38361.1.
    BT006880 mRNA. Translation: AAP35526.1.
    BC011976 mRNA. Translation: AAH11976.1.
    CCDSiCCDS47074.1.
    PIRiS13669. A28414.
    RefSeqiNP_001502.1. NM_001511.3.
    UniGeneiHs.708652.
    Hs.789.

    Genome annotation databases

    EnsembliENST00000395761; ENSP00000379110; ENSG00000163739.
    GeneIDi2919.
    KEGGihsa:2919.
    UCSCiuc003hhh.3. human.

    Polymorphism databases

    DMDMi121622.

    Cross-referencesi

    Web resourcesi

    Wikipedia

    CXCL1 entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J03561 mRNA. Translation: AAA35933.1 .
    X12510 mRNA. Translation: CAA31027.1 .
    X54489 Genomic DNA. Translation: CAA38361.1 .
    BT006880 mRNA. Translation: AAP35526.1 .
    BC011976 mRNA. Translation: AAH11976.1 .
    CCDSi CCDS47074.1.
    PIRi S13669. A28414.
    RefSeqi NP_001502.1. NM_001511.3.
    UniGenei Hs.708652.
    Hs.789.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MGS NMR - A/B 35-107 [» ]
    1MSG NMR - A/B 35-106 [» ]
    1MSH NMR - A/B 35-106 [» ]
    1ROD NMR - A/B 40-62 [» ]
    A/B 89-107 [» ]
    ProteinModelPortali P09341.
    SMRi P09341. Positions 35-107.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109176. 3 interactions.
    DIPi DIP-5896N.
    IntActi P09341. 1 interaction.
    STRINGi 9606.ENSP00000379110.

    PTM databases

    PhosphoSitei P09341.

    Polymorphism databases

    DMDMi 121622.

    Proteomic databases

    MaxQBi P09341.
    PaxDbi P09341.
    PRIDEi P09341.

    Protocols and materials databases

    DNASUi 2919.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000395761 ; ENSP00000379110 ; ENSG00000163739 .
    GeneIDi 2919.
    KEGGi hsa:2919.
    UCSCi uc003hhh.3. human.

    Organism-specific databases

    CTDi 2919.
    GeneCardsi GC04P074724.
    H-InvDB HIX0120073.
    HGNCi HGNC:4602. CXCL1.
    MIMi 155730. gene.
    neXtProti NX_P09341.
    PharmGKBi PA35050.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG266004.
    HOGENOMi HOG000220915.
    HOVERGENi HBG107789.
    InParanoidi P09341.
    KOi K05505.
    OrthoDBi EOG7NSB57.
    PhylomeDBi P09341.
    TreeFami TF333433.

    Enzyme and pathway databases

    Reactomei REACT_15344. Chemokine receptors bind chemokines.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    ChiTaRSi CXCL1. human.
    EvolutionaryTracei P09341.
    GeneWikii CXCL1.
    GenomeRNAii 2919.
    NextBioi 11573.
    PMAP-CutDB P09341.
    PROi P09341.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P09341.
    Bgeei P09341.
    CleanExi HS_CXCL1.
    Genevestigatori P09341.

    Family and domain databases

    InterProi IPR001089. Chemokine_CXC.
    IPR018048. Chemokine_CXC_CS.
    IPR001811. Chemokine_IL8-like_dom.
    [Graphical view ]
    PANTHERi PTHR10179. PTHR10179. 1 hit.
    Pfami PF00048. IL8. 1 hit.
    [Graphical view ]
    PRINTSi PR00437. SMALLCYTKCXC.
    SMARTi SM00199. SCY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54117. SSF54117. 1 hit.
    PROSITEi PS00471. SMALL_CYTOKINES_CXC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells."
      Anisowicz A., Bardwell L., Sager R.
      Proc. Natl. Acad. Sci. U.S.A. 84:7188-7192(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to beta-thromboglobulin."
      Richmond A., Balentien E., Thomas H.G., Flaggs G., Barton D.E., Spiess J., Bordoni R., Francke U., Derynck R.
      EMBO J. 7:2025-2033(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Nucleotide sequence of the human melanoma growth stimulatory activity (MGSA) gene."
      Baker N.E., Kucera G., Richmond A.
      Nucleic Acids Res. 18:6453-6453(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Blood.
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    6. "Isolation of the CXC chemokines ENA-78, GRO alpha and GRO gamma from tumor cells and leukocytes reveals NH2-terminal heterogeneity. Functional comparison of different natural isoforms."
      Wuyts A., Govaerts C., Struyf S., Lenaerts J.-P., Put W., Conings R., Proost P., Van Damme J.
      Eur. J. Biochem. 260:421-429(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 35-107, IDENTIFICATION OF GRO-ALPHA(4-73); GRO-ALPHA(5-73) AND GRO-ALPHA(6-73), PROTEOLYTIC PROCESSING OF N-TERMINAL, FUNCTION.
      Tissue: Peripheral blood monocyte.
    7. "Lipopolysaccharide-stimulated human monocytes secrete, apart from neutrophil-activating peptide 1/interleukin 8, a second neutrophil-activating protein. NH2-terminal amino acid sequence identity with melanoma growth stimulatory activity."
      Schroeder J.-M., Persoon N.L.M., Christophers E.
      J. Exp. Med. 171:1091-1100(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 35-65.
    8. "Inflammatory cytokines induce synthesis and secretion of gro protein and a neutrophil chemotactic factor but not beta 2-microglobulin in human synovial cells and fibroblasts."
      Golds E.E., Mason P., Nyirkos P.
      Biochem. J. 259:585-588(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 35-57.
    9. "Biochemical and biological characterization of NAP-1/IL-8-related cytokines in lesional psoriatic scale."
      Schroeder J.-M.
      Adv. Exp. Med. Biol. 305:97-107(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 35-51.
      Tissue: Skin.
    10. "Signal peptide prediction based on analysis of experimentally verified cleavage sites."
      Zhang Z., Henzel W.J.
      Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 35-49.
    11. "Expression and secretion of gro/MGSA by stimulated human endothelial cells."
      Wen D., Rowland A., Derynck R.
      EMBO J. 8:1761-1766(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE FUNCTION.
    12. "1H assignment and secondary structure determination of human melanoma growth stimulating activity (MGSA) by NMR spectroscopy."
      Fairbrother W.J., Reilly D., Colby T., Horuk R.
      FEBS Lett. 330:302-306(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    13. "The solution structure of melanoma growth stimulating activity."
      Fairbrother W.J., Reilly D., Colby T., Hesselgesser J., Horuk R.
      J. Mol. Biol. 242:252-270(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    14. "Solution structure of GRO/melanoma growth stimulatory activity determined by 1H NMR spectroscopy."
      Kim K.S., Clark-Lewis I., Sykes B.D.
      J. Biol. Chem. 269:32909-32915(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiGROA_HUMAN
    AccessioniPrimary (citable) accession number: P09341
    Secondary accession number(s): Q9UCR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 156 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3