Reviewed, UniProtKB/Swiss-Prot P09339 (ACON_BACSU)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	citB
Ordered Locus Names:	BSU18000

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	909 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Monomer.
Induction	By citrate, decoyinine and nutrient depletion.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 909	908	Aconitate hydratase		PRO_0000076655
Sites
	Metal binding	450	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	516	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	519	1	Iron-sulfur (4Fe-4S) By similarity
Experimental info
	Sequence conflict	43 – 45	3	SKL → FEA AA sequence Ref.4
	Sequence conflict	119	1	G → V Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P09339-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 9545E734F2BCF735	FASTA	909	99,334
	10 20 30 40 50 60 MANEQKTAAK DVFQARKTFT TNGKTYHYYS LKALEDSGIG KVSKLPYSIK VLLESVLRQV 70 80 90 100 110 120 DGFVIKKEHV ENLAKWGTAE LKDIDVPFKP SRVILQDFTG VPAVVDLASL RKAMAAVGGD 130 140 150 160 170 180 PDKINPEIPV DLVIDHSVQV DKAGTEDALA VNMDLEFERN AERYKFLSWA KKAFNNYQAV 190 200 210 220 230 240 PPATGIVHQV NLEFLASVVH AIEEDGELVT YPDTLVGTDS HTTMINGIGV LGWGVGGIEA 250 260 270 280 290 300 EAGMLGQPSY FPVPEVIGAK LVGKLPNGTT ATDLALKVTQ VLREKGVVGK FVEFFGPGIA 310 320 330 340 350 360 ELPLADRATI ANMAPEYGAT CGFFPVDEEA LNYLRLTGRD PEHIDVVEAY CRSNGLFYTP 370 380 390 400 410 420 DAEDPQFTDV VEIDLSQIEA NLSGPKRPQD LIPLSAMQET FKKQLVSPAG NQGFGLNAEE 430 440 450 460 470 480 ENKEIKFKLL NGEETVMKTG AIAIAAITSC TNTSNPYVLI GAGLVAKKAV ELGLKVPNYV 490 500 510 520 530 540 KTSLAPGSKV VTGYLVNSGL LPYMKELGFN LVGYGCTTCI GNSGPLSPEI EEAVAKNDLL 550 560 570 580 590 600 ITSVLSGNRN FEGRIHPLVK GNYLASPPLV VAYALAGTVN INLKTDPIGV GKDGQNVYFN 610 620 630 640 650 660 DIWPSMDEIN ALVKQTVTPE LFRKEYETVF DDNKRWNEIE TTDEALYKWD NDSTYIQNPP 670 680 690 700 710 720 FFEEMSVEPG KVEPLKGLRV VGKFGDSVTT DHISPAGAIG KDTPAGKYLQ EKGVSPRDFN 730 740 750 760 770 780 SYGSRRGNHE VMMRGTFANI RIKNQIAPGT EGGFTTYWPT GEVTSIYDAC MKYKEDKTGL 790 800 810 820 830 840 VVLAGKDYGM GSSRDWAAKG TNLLGIRTVI AESFERIHRS NLVFMGVLPL QFKQGENADT 850 860 870 880 890 900 LGLTGKEVIE VDVDETVRPR DLVTVRAINE DGNVTTFEAV VRFDSEVEID YYRHGGILQM VLREKMKQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporter." Rose M., Entian K.-D. Microbiology 142:3097-3101(1996) [PubMed: 8969507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	Wilde R.J. Thesis (1988), University of Sheffield, United Kingdom Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-141.
[4]	"Purification of aconitase from Bacillus subtilis and correlation of its N-terminal amino acid sequence with the sequence of the citB gene." Dingman D.W., Sonenshein A.L. J. Bacteriol. 169:3062-3067(1987) [PubMed: 3110133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45, PROTEIN SEQUENCE OF 2-8.
[5]	Sonenshein A.L. Submitted (JUL-1987) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73234 Genomic DNA. Translation: CAA97599.1. AL009126 Genomic DNA. Translation: CAB13684.1. M16776 Genomic DNA. Translation: AAA22316.1.
PIR	G69599.
RefSeq	NP_389683.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938140.
GenomeReviews	Gene locus BSU18000 in contig AL009126_GR.
KEGG	bsu:BSU18000.
NMPDR	fig|224308.1.peg.1804.
Organism-specific databases
SubtiList	BG10478. citB. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P09339.
OMA	RIKNEML.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1801-MON.
BRENDA	4.2.1.3. 150.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_BACSU

Accession

Primary (citable) accession number: P09339
Secondary accession number(s): Q45059

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 80 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)