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Protein

Exfoliative toxin A

Gene

eta

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has serine protease-like properties and binds to the skin protein profilaggrin. Cleaves substrates after acidic residues. Exfoliative toxins cause impetigous diseases commonly referred as staphylococcal scalded skin syndrome (SSSS).2 Publications

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101Charge relay system
Active sitei158 – 1581Charge relay system
Active sitei233 – 2331Charge relay system

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease, Toxin

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiS01.270.

Names & Taxonomyi

Protein namesi
Recommended name:
Exfoliative toxin A (EC:3.4.21.-)
Alternative name(s):
Epidermolytic toxin A
Gene namesi
Name:eta
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi233 – 2331S → G: Loss of toxicity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3838Add
BLAST
Chaini39 – 280242Exfoliative toxin APRO_0000026905Add
BLAST

Structurei

Secondary structure

1
280
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi42 – 5615Combined sources
Helixi60 – 623Combined sources
Turni65 – 673Combined sources
Beta strandi68 – 703Combined sources
Helixi75 – 773Combined sources
Helixi81 – 833Combined sources
Beta strandi84 – 896Combined sources
Turni90 – 923Combined sources
Beta strandi93 – 997Combined sources
Beta strandi101 – 1077Combined sources
Helixi109 – 1124Combined sources
Helixi113 – 1153Combined sources
Helixi119 – 1213Combined sources
Beta strandi122 – 1265Combined sources
Beta strandi143 – 1508Combined sources
Beta strandi160 – 1645Combined sources
Helixi173 – 1764Combined sources
Helixi185 – 1873Combined sources
Beta strandi193 – 1986Combined sources
Turni201 – 2033Combined sources
Beta strandi209 – 2157Combined sources
Helixi218 – 2203Combined sources
Beta strandi221 – 2255Combined sources
Helixi230 – 2323Combined sources
Beta strandi236 – 2383Combined sources
Beta strandi242 – 25312Combined sources
Beta strandi260 – 2667Combined sources
Helixi269 – 27810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AGJX-ray1.70A/B39-280[»]
1DUAX-ray2.00A39-280[»]
1DUEX-ray2.00A39-280[»]
1EXFX-ray2.10A39-280[»]
ProteinModelPortaliP09331.
SMRiP09331. Positions 39-280.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09331.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S1B family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR008256. Peptidase_S1B.
IPR008353. Peptidase_S1B_tx.
IPR001254. Trypsin_dom.
IPR028301. V8_his_AS.
IPR000126. V8_ser_AS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR01774. EXFOLTOXIN.
PR00839. V8PROTEASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00672. V8_HIS. 1 hit.
PS00673. V8_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSKIISKV LLSLSLFTVG ASAFVIQDEL MQKNHAKAEV SAEEIKKHEE
60 70 80 90 100
KWNKYYGVNA FNLPKELFSK VDEKDRQKYP YNTIGNVFVK GQTSATGVLI
110 120 130 140 150
GKNTVLTNRH IAKFANGDPS KVSFRPSINT DDNGNTETPY GEYEVKEILQ
160 170 180 190 200
EPFGAGVDLA LIRLKPDQNG VSLGDKISPA KIGTSNDLKD GDKLELIGYP
210 220 230 240 250
FDHKVNQMHR SEIELTTLSR GLRYYGFTVP GNSGSGIFNS NGELVGIHSS
260 270 280
KVSHLDREHQ INYGVGIGNY VKRIINEKNE
Length:280
Mass (Da):31,077
Last modified:July 1, 1989 - v1
Checksum:i4FBAF750FFE43586
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17357 Genomic DNA. Translation: AAA26626.1.
M17347 Genomic DNA. Translation: AAA26625.1.
L25372 Genomic DNA. Translation: AAA17490.1.
PIRiA26680. PRSAEA.
RefSeqiWP_001065781.1. NZ_LJBM01000002.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17357 Genomic DNA. Translation: AAA26626.1.
M17347 Genomic DNA. Translation: AAA26625.1.
L25372 Genomic DNA. Translation: AAA17490.1.
PIRiA26680. PRSAEA.
RefSeqiWP_001065781.1. NZ_LJBM01000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AGJX-ray1.70A/B39-280[»]
1DUAX-ray2.00A39-280[»]
1DUEX-ray2.00A39-280[»]
1EXFX-ray2.10A39-280[»]
ProteinModelPortaliP09331.
SMRiP09331. Positions 39-280.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP09331.
PROiP09331.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR008256. Peptidase_S1B.
IPR008353. Peptidase_S1B_tx.
IPR001254. Trypsin_dom.
IPR028301. V8_his_AS.
IPR000126. V8_ser_AS.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR01774. EXFOLTOXIN.
PR00839. V8PROTEASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00672. V8_HIS. 1 hit.
PS00673. V8_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence determination and comparison of the exfoliative toxin A and toxin B genes from Staphylococcus aureus."
    Lee C.Y., Schmidt J.J., Johnson-Winegar A.D., Spero L., Iandolo J.J.
    J. Bacteriol. 169:3904-3909(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: UT0002.
  2. "Nucleotide sequence of the epidermolytic toxin A gene of Staphylococcus aureus."
    O'Toole P.W., Foster T.J.
    J. Bacteriol. 169:3910-3915(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: TC16.
  3. "DNA sequencing of the eta gene coding for staphylococcal exfoliative toxin serotype A."
    Sakurai S., Suzuki H., Kondo I.
    J. Gen. Microbiol. 134:711-717(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ZM.
  4. "The reactive serine residue of epidermolytic toxin A."
    Bailey C.J., Smith T.P.
    Biochem. J. 269:535-537(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: FUNCTION.
  6. "The role of the serine protease active site in the mode of action of epidermolytic toxin of Staphylococcus aureus."
    Redpath M.B., Foster T.J., Bailey C.J.
    FEMS Microbiol. Lett. 65:151-155(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-233.
  7. "The structure of the superantigen exfoliative toxin A suggests a novel regulation as a serine protease."
    Vath G.M., Earhart C.A., Rago J.V., Kim M.H., Bohach G.A., Schlievert P.M., Ohlendorf D.H.
    Biochemistry 36:1559-1566(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
    Strain: MNEV.
  8. "The structure of Staphylococcus aureus epidermolytic toxin A, an atypic serine protease, at 1.7-A resolution."
    Cavarelli J., Prevost G., Bourguet W., Moulinier L., Chevrier B., Delagoutte B., Bilwes A., Mourey L., Rifai S., Piemont Y., Moras D.
    Structure 5:813-824(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Entry informationi

Entry nameiETA_STAAU
AccessioniPrimary (citable) accession number: P09331
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 11, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.