P09331 (ETA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exfoliative toxin A
EC=3.4.21.-

Alternative name(s):
Epidermolytic toxin A

Gene names

Name:

eta

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has serine protease-like properties and binds to the skin protein profilaggrin. Cleaves substrates after acidic residues. Exfoliative toxins cause impetigous diseases commonly referred as staphylococcal scalded skin syndrome (SSSS). Ref.4 Ref.5
Cofactor	Calcium.
Sequence similarities	Belongs to the peptidase S1B family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium
Molecular function	Hydrolase Protease Serine protease Toxin
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 38

38

Chain

39 – 280

242

Exfoliative toxin A

PRO_0000026905

Sites

Active site

110

1

Charge relay system

Active site

158

1

Charge relay system

Active site

233

1

Charge relay system

Experimental info

Mutagenesis

233

1

S → G: Loss of toxicity. Ref.6

Secondary structure

1

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280

Helix Strand Turn

Details...

Helix

42 – 56

15

Helix

60 – 62

3

Turn

65 – 67

3

Beta strand

68 – 70

3

Helix

75 – 77

3

Helix

81 – 83

3

Beta strand

84 – 89

6

Turn

90 – 92

3

Beta strand

93 – 99

7

Beta strand

101 – 107

7

Helix

109 – 112

4

Helix

113 – 115

3

Helix

119 – 121

3

Beta strand

122 – 126

5

Beta strand

143 – 150

8

Beta strand

160 – 164

5

Helix

173 – 176

4

Beta strand

193 – 198

6

Turn

201 – 203

3

Beta strand

209 – 215

7

Helix

218 – 220

3

Beta strand

221 – 225

5

Helix

230 – 232

3

Beta strand

236 – 238

3

Beta strand

242 – 253

12

Beta strand

260 – 266

7

Helix

269 – 278

10

Sequences

	Sequence		Length	Mass (Da)
	P09331 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 4FBAF750FFE43586	FASTA	280	31,077
	10 20 30 40 50 60 MNNSKIISKV LLSLSLFTVG ASAFVIQDEL MQKNHAKAEV SAEEIKKHEE KWNKYYGVNA 70 80 90 100 110 120 FNLPKELFSK VDEKDRQKYP YNTIGNVFVK GQTSATGVLI GKNTVLTNRH IAKFANGDPS 130 140 150 160 170 180 KVSFRPSINT DDNGNTETPY GEYEVKEILQ EPFGAGVDLA LIRLKPDQNG VSLGDKISPA 190 200 210 220 230 240 KIGTSNDLKD GDKLELIGYP FDHKVNQMHR SEIELTTLSR GLRYYGFTVP GNSGSGIFNS 250 260 270 280 NGELVGIHSS KVSHLDREHQ INYGVGIGNY VKRIINEKNE

References

[1]	"Sequence determination and comparison of the exfoliative toxin A and toxin B genes from Staphylococcus aureus." Lee C.Y., Schmidt J.J., Johnson-Winegar A.D., Spero L., Iandolo J.J. J. Bacteriol. 169:3904-3909(1987) [PubMed: 3040666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: UT0002.
[2]	"Nucleotide sequence of the epidermolytic toxin A gene of Staphylococcus aureus." O'Toole P.W., Foster T.J. J. Bacteriol. 169:3910-3915(1987) [PubMed: 3040667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TC16.
[3]	"DNA sequencing of the eta gene coding for staphylococcal exfoliative toxin serotype A." Sakurai S., Suzuki H., Kondo I. J. Gen. Microbiol. 134:711-717(1988) [PubMed: 3183619] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ZM.
[4]	"The reactive serine residue of epidermolytic toxin A." Bailey C.J., Smith T.P. Biochem. J. 269:535-537(1990) [PubMed: 2117445] [Abstract] Cited for: FUNCTION.
[5]	"The epidermolytic toxins are serine proteases." Dancer S.J., Garrat R., Saldanha J., Jhoti H., Evans R. FEBS Lett. 268:129-132(1990) [PubMed: 2384148] [Abstract] Cited for: FUNCTION.
[6]	"The role of the serine protease active site in the mode of action of epidermolytic toxin of Staphylococcus aureus." Redpath M.B., Foster T.J., Bailey C.J. FEMS Microbiol. Lett. 65:151-155(1991) [PubMed: 1884990] [Abstract] Cited for: MUTAGENESIS OF SER-233.
[7]	"The structure of the superantigen exfoliative toxin A suggests a novel regulation as a serine protease." Vath G.M., Earhart C.A., Rago J.V., Kim M.H., Bohach G.A., Schlievert P.M., Ohlendorf D.H. Biochemistry 36:1559-1566(1997) [PubMed: 9048539] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS). Strain: MNEV.
[8]	"The structure of Staphylococcus aureus epidermolytic toxin A, an atypic serine protease, at 1.7-A resolution." Cavarelli J., Prevost G., Bourguet W., Moulinier L., Chevrier B., Delagoutte B., Bilwes A., Mourey L., Rifai S., Piemont Y., Moras D. Structure 5:813-824(1997) [PubMed: 9261066] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17357 Genomic DNA. Translation: AAA26626.1.
M17347 Genomic DNA. Translation: AAA26625.1.
L25372 Genomic DNA. Translation: AAA17490.1.

PIR

PRSAEA. A26680.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AGJ	X-ray	1.70	A/B	39-280	[»]
1DUA	X-ray	2.00	A	39-280	[»]
1DUE	X-ray	2.00	A	39-280	[»]
1EXF	X-ray	2.10	A	39-280	[»]

ProteinModelPortal

P09331.

SMR

P09331. Positions 39-280.

ModBase

Search...

Protein family/group databases

MEROPS

S01.270.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009003. Pept_cys/ser_Trypsin-like.
IPR000126. Pept_S1B_AS.
IPR001254. Peptidase_S1_S6.
IPR008256. Peptidase_S1B.
IPR008353. Peptidase_S1B_tx.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR01774. EXFOLTOXIN.
PR00839. V8PROTEASE.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

SUPFAM

SSF50494. Pept_Ser_Cys. 1 hit.

PROSITE

PS00672. V8_HIS. 1 hit.
PS00673. V8_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ETA_STAAU

Accession

Primary (citable) accession number: P09331

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program