P09326 (CD48_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD48 antigen

Alternative name(s):
B-lymphocyte activation marker BLAST-1
BCM1 surface antigen
Leukocyte antigen MEM-102
TCT.1
CD_antigen=CD48

Gene names

Name:	CD48
Synonyms:	BCM1, BLAST1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Sequence similarities	Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	blood coagulation Traceable author statement. Source: Reactome defense response Traceable author statement. Source: ProtInc leukocyte migration Traceable author statement. Source: Reactome
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc membrane raft Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CD244	Q9BZW8	2	EBI-714770,EBI-1580565

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Potential

Chain

27 – 220

194

CD48 antigen

PRO_0000014881

Propeptide

221 – 243

23

Removed in mature form

PRO_0000014882

Regions

Domain

29 – 127

99

Ig-like C2-type 1

Domain

132 – 212

81

Ig-like C2-type 2

Amino acid modifications

Lipidation

220

1

GPI-anchor amidated serine

Glycosylation

40

1

N-linked (GlcNAc...) Potential

Glycosylation

44

1

N-linked (GlcNAc...) Potential

Glycosylation

104

1

N-linked (GlcNAc...) Ref.10

Glycosylation

162

1

N-linked (GlcNAc...) Potential

Glycosylation

178

1

N-linked (GlcNAc...) Ref.10

Glycosylation

189

1

N-linked (GlcNAc...) Ref.10

Disulfide bond

154 ↔ 196

Potential

Natural variations

Natural variant

102

1

E → Q.
Corresponds to variant rs2295615 [ dbSNP | Ensembl ].

VAR_020082

Natural variant

241

1

L → S.
Corresponds to variant rs16832307 [ dbSNP | Ensembl ].

VAR_049909

Experimental info

Sequence conflict

2

1

C → W in AAA62834. Ref.1

Sequence conflict

66

1

I → N in CAA29647. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

243

Helix Strand Turn

Details...

Beta strand

26 – 28

3

Beta strand

34 – 36

3

Beta strand

41 – 43

3

Beta strand

51 – 61

11

Beta strand

64 – 69

6

Beta strand

74 – 76

3

Turn

80 – 84

5

Turn

89 – 91

3

Beta strand

94 – 98

5

Helix

101 – 103

3

Beta strand

105 – 113

9

Turn

114 – 116

3

Beta strand

117 – 120

4

Beta strand

126 – 128

3

Sequences

	Sequence		Length	Mass (Da)
	P09326 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 9E46D76FC36A512C	FASTA	243	27,683
	10 20 30 40 50 60 MCSRGWDSCL ALELLLLPLS LLVTSIQGHL VHMTVVSGSN VTLNISESLP ENYKQLTWFY 70 80 90 100 110 120 TFDQKIVEWD SRKSKYFESK FKGRVRLDPQ SGALYISKVQ KEDNSTYIMR VLKKTGNEQE 130 140 150 160 170 180 WKIKLQVLDP VPKPVIKIEK IEDMDDNCYL KLSCVIPGES VNYTWYGDKR PFPKELQNSV 190 200 210 220 230 240 LETTLMPHNY SRCYTCQVSN SVSSKNGTVC LSPPCTLARS FGVEWIASWL VVTVPTILGL LLT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The isolation of cDNA clones for CD48." Vaughan H.A., Henning M.M., Purcell D.F.J., McKenzie I.F.C., Sandrin M.S. Immunogenetics 33:113-117(1991) [PubMed: 1999351] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1." Korinek V., Stefanova I., Angelisova P., Hilbert I., Horejsi V. Immunogenetics 33:108-112(1991) [PubMed: 1999350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Molecular cloning of the lymphocyte activation marker Blast-1." Staunton D.E., Thorley-Lawson D.A. EMBO J. 6:3695-3701(1987) [PubMed: 2828034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell.
[8]	"Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48)." Fisher R.C., Thorley-Lawson D.A. Mol. Cell. Biol. 11:1614-1623(1991) [PubMed: 1847502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
[9]	"TCT.1, a target molecule for gamma/delta T cells, is encoded by an immunoglobulin superfamily gene (Blast-1) located in the CD1 region of human chromosome 1." del Porto P., Mami-Chouaib F., Bruneau J.-M., Jitsukawa S., Dumas J., Harnois M., Hercend T. J. Exp. Med. 173:1339-1344(1991) [PubMed: 1827826] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[10]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104; ASN-178 AND ASN-189, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"Solution structure of the first Ig-like domain from human CD48 antigen." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 24-140.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M59904 mRNA. Translation: AAA62834.1.
X06341 mRNA. Translation: CAA29647.1.
M37766 mRNA. Translation: AAA36211.1.
BT019813 mRNA. Translation: AAV38616.1.
AL121985 Genomic DNA. Translation: CAC00578.1.
CH471121 Genomic DNA. Translation: EAW52705.1.
BC016182 mRNA. Translation: AAH16182.1.
M63911 Genomic DNA. Translation: AAA35602.1.

IPI

IPI00013831.

PIR

A53244.

RefSeq

NP_001769.2. NM_001778.2.

UniGene

Hs.243564.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EDO	NMR	-	A	27-140	[»]

ProteinModelPortal

P09326.

SMR

P09326. Positions 29-214.

ModBase

Search...

Protein-protein interaction databases

IntAct

P09326. 4 interactions.

MINT

MINT-1427578.

STRING

P09326.

Polymorphism databases

DMDM

114871.

Proteomic databases

PeptideAtlas

P09326.

PRIDE

P09326.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000368046; ENSP00000357025; ENSG00000117091.

GeneID

962.

KEGG

hsa:962.

UCSC

uc001fwn.1. human.

Organism-specific databases

CTD

962.

GeneCards

GC01M160648.

H-InvDB

HIX0001222.

HGNC

HGNC:1683. CD48.

HPA

CAB002497.

MIM

109530. gene.

neXtProt

NX_P09326.

PharmGKB

PA26223.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG09219.

HOGENOM

HBG125870.

HOVERGEN

HBG004490.

InParanoid

P09326.

OMA

KLSCVIP.

OrthoDB

EOG42RD83.

PhylomeDB

P09326.

Enzyme and pathway databases

Reactome

REACT_604. Hemostasis.

Gene expression databases

ArrayExpress

P09326.

Bgee

P09326.

CleanEx

HS_CD48.

Genevestigator

P09326.

GermOnline

ENSG00000117091. Homo sapiens.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR015631. SLAM_fam_rcpts.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 2 hits.

KO

K06479.

PANTHER

PTHR12080. SLAM_related. 1 hit.

Pfam

PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

4020.

SOURCE

Search...

Entry information

Entry name

CD48_HUMAN

Accession

Primary (citable) accession number: P09326
Secondary accession number(s): Q5U055

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1991
Last modified:	January 25, 2012
This is version 138 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.