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P09326

- CD48_HUMAN

UniProt

P09326 - CD48_HUMAN

Protein

CD48 antigen

Gene

CD48

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 166 (01 Oct 2014)
      Sequence version 2 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

    GO - Molecular functioni

    1. antigen binding Source: Ensembl
    2. protein binding Source: UniProtKB
    3. receptor activity Source: InterPro

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. defense response Source: ProtInc
    3. leukocyte migration Source: Reactome
    4. mast cell activation Source: Ensembl
    5. signal transduction Source: Ensembl
    6. T cell activation Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_12051. Cell surface interactions at the vascular wall.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CD48 antigen
    Alternative name(s):
    B-lymphocyte activation marker BLAST-1
    BCM1 surface antigen
    Leukocyte antigen MEM-102
    SLAM family member 2
    Short name:
    SLAMF2
    Signaling lymphocytic activation molecule 2
    TCT.1
    CD_antigen: CD48
    Gene namesi
    Name:CD48
    Synonyms:BCM1, BLAST1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:1683. CD48.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of plasma membrane Source: Ensembl
    2. external side of plasma membrane Source: Ensembl
    3. extracellular vesicular exosome Source: UniProt
    4. integral component of membrane Source: InterPro
    5. integral component of plasma membrane Source: ProtInc
    6. membrane Source: UniProtKB
    7. membrane raft Source: UniProtKB
    8. plasma membrane Source: Reactome
    9. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26223.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 220194CD48 antigenPRO_0000014881Add
    BLAST
    Propeptidei221 – 24323Removed in mature formBy similarityPRO_0000014882Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi104 – 1041N-linked (GlcNAc...)1 Publication
    Disulfide bondi154 ↔ 196PROSITE-ProRule annotation
    Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi178 – 1781N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi189 – 1891N-linked (GlcNAc...)1 Publication
    Lipidationi220 – 2201GPI-anchor amidated serineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiP09326.
    PaxDbiP09326.
    PeptideAtlasiP09326.
    PRIDEiP09326.

    PTM databases

    PhosphoSiteiP09326.

    Expressioni

    Gene expression databases

    ArrayExpressiP09326.
    BgeeiP09326.
    CleanExiHS_CD48.
    GenevestigatoriP09326.

    Organism-specific databases

    HPAiCAB002497.

    Interactioni

    Subunit structurei

    Interacts with CD244 in an heterophilic manner.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CD244Q9BZW82EBI-714770,EBI-1580565

    Protein-protein interaction databases

    BioGridi107400. 4 interactions.
    IntActiP09326. 4 interactions.
    MINTiMINT-1427578.
    STRINGi9606.ENSP00000357025.

    Structurei

    Secondary structure

    1
    243
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 363
    Beta strandi41 – 433
    Beta strandi51 – 6111
    Beta strandi64 – 696
    Beta strandi74 – 763
    Turni80 – 845
    Turni89 – 913
    Beta strandi94 – 985
    Helixi101 – 1033
    Beta strandi105 – 1139
    Turni114 – 1163
    Beta strandi117 – 1204
    Beta strandi126 – 1283

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2EDONMR-A27-140[»]
    ProteinModelPortaliP09326.
    SMRiP09326. Positions 29-140.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09326.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 12799Ig-like C2-type 1Add
    BLAST
    Domaini132 – 21281Ig-like C2-type 2Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG40265.
    HOGENOMiHOG000111487.
    HOVERGENiHBG004490.
    InParanoidiP09326.
    KOiK06479.
    OMAiAWIATWL.
    OrthoDBiEOG7JQBPS.
    PhylomeDBiP09326.
    TreeFamiTF334964.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    InterProiIPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    IPR015631. SLAM_fam_rcpts.
    [Graphical view]
    PANTHERiPTHR12080. PTHR12080. 1 hit.
    PfamiPF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P09326-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCSRGWDSCL ALELLLLPLS LLVTSIQGHL VHMTVVSGSN VTLNISESLP    50
    ENYKQLTWFY TFDQKIVEWD SRKSKYFESK FKGRVRLDPQ SGALYISKVQ 100
    KEDNSTYIMR VLKKTGNEQE WKIKLQVLDP VPKPVIKIEK IEDMDDNCYL 150
    KLSCVIPGES VNYTWYGDKR PFPKELQNSV LETTLMPHNY SRCYTCQVSN 200
    SVSSKNGTVC LSPPCTLARS FGVEWIASWL VVTVPTILGL LLT 243
    Length:243
    Mass (Da):27,683
    Last modified:November 1, 1991 - v2
    Checksum:i9E46D76FC36A512C
    GO
    Isoform 2 (identifier: P09326-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         129-169: DPVPKPVIKI...SVNYTWYGDK → GESGEPKSKS...EEERKTSGQV
         170-243: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:169
    Mass (Da):19,429
    Checksum:i0E528B574BD04C5B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21C → W in AAA62834. (PubMed:1999351)Curated
    Sequence conflicti66 – 661I → N in CAA29647. (PubMed:2828034)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti102 – 1021E → Q.
    Corresponds to variant rs2295615 [ dbSNP | Ensembl ].
    VAR_020082
    Natural varianti241 – 2411L → S.
    Corresponds to variant rs16832307 [ dbSNP | Ensembl ].
    VAR_049909

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei129 – 16941DPVPK…WYGDK → GESGEPKSKSPLQWPQMDHC RASWEAWGTLGEEERKTSGQ V in isoform 2. 1 PublicationVSP_055598Add
    BLAST
    Alternative sequencei170 – 24374Missing in isoform 2. 1 PublicationVSP_055599Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59904 mRNA. Translation: AAA62834.1.
    X06341 mRNA. Translation: CAA29647.1.
    M37766 mRNA. Translation: AAA36211.1.
    BT019813 mRNA. Translation: AAV38616.1.
    AL121985 Genomic DNA. Translation: CAC00578.1.
    CH471121 Genomic DNA. Translation: EAW52705.1.
    BC016182 mRNA. Translation: AAH16182.1.
    BC030224 mRNA. Translation: AAH30224.1.
    M63911 Genomic DNA. Translation: AAA35602.1.
    CCDSiCCDS1208.1.
    PIRiA53244.
    RefSeqiNP_001769.2. NM_001778.3.
    UniGeneiHs.243564.

    Genome annotation databases

    EnsembliENST00000368045; ENSP00000357024; ENSG00000117091. [P09326-2]
    ENST00000368046; ENSP00000357025; ENSG00000117091. [P09326-1]
    GeneIDi962.
    KEGGihsa:962.
    UCSCiuc001fwn.4. human.

    Polymorphism databases

    DMDMi114871.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59904 mRNA. Translation: AAA62834.1 .
    X06341 mRNA. Translation: CAA29647.1 .
    M37766 mRNA. Translation: AAA36211.1 .
    BT019813 mRNA. Translation: AAV38616.1 .
    AL121985 Genomic DNA. Translation: CAC00578.1 .
    CH471121 Genomic DNA. Translation: EAW52705.1 .
    BC016182 mRNA. Translation: AAH16182.1 .
    BC030224 mRNA. Translation: AAH30224.1 .
    M63911 Genomic DNA. Translation: AAA35602.1 .
    CCDSi CCDS1208.1.
    PIRi A53244.
    RefSeqi NP_001769.2. NM_001778.3.
    UniGenei Hs.243564.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2EDO NMR - A 27-140 [» ]
    ProteinModelPortali P09326.
    SMRi P09326. Positions 29-140.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107400. 4 interactions.
    IntActi P09326. 4 interactions.
    MINTi MINT-1427578.
    STRINGi 9606.ENSP00000357025.

    PTM databases

    PhosphoSitei P09326.

    Polymorphism databases

    DMDMi 114871.

    Proteomic databases

    MaxQBi P09326.
    PaxDbi P09326.
    PeptideAtlasi P09326.
    PRIDEi P09326.

    Protocols and materials databases

    DNASUi 962.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000368045 ; ENSP00000357024 ; ENSG00000117091 . [P09326-2 ]
    ENST00000368046 ; ENSP00000357025 ; ENSG00000117091 . [P09326-1 ]
    GeneIDi 962.
    KEGGi hsa:962.
    UCSCi uc001fwn.4. human.

    Organism-specific databases

    CTDi 962.
    GeneCardsi GC01M160648.
    HGNCi HGNC:1683. CD48.
    HPAi CAB002497.
    MIMi 109530. gene.
    neXtProti NX_P09326.
    PharmGKBi PA26223.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG40265.
    HOGENOMi HOG000111487.
    HOVERGENi HBG004490.
    InParanoidi P09326.
    KOi K06479.
    OMAi AWIATWL.
    OrthoDBi EOG7JQBPS.
    PhylomeDBi P09326.
    TreeFami TF334964.

    Enzyme and pathway databases

    Reactomei REACT_12051. Cell surface interactions at the vascular wall.

    Miscellaneous databases

    EvolutionaryTracei P09326.
    GeneWikii CD48.
    GenomeRNAii 962.
    NextBioi 4020.
    PROi P09326.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P09326.
    Bgeei P09326.
    CleanExi HS_CD48.
    Genevestigatori P09326.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    InterProi IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    IPR015631. SLAM_fam_rcpts.
    [Graphical view ]
    PANTHERi PTHR12080. PTHR12080. 1 hit.
    Pfami PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1."
      Korinek V., Stefanova I., Angelisova P., Hilbert I., Horejsi V.
      Immunogenetics 33:108-112(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Molecular cloning of the lymphocyte activation marker Blast-1."
      Staunton D.E., Thorley-Lawson D.A.
      EMBO J. 6:3695-3701(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: B-cell and Pancreas.
    8. "Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48)."
      Fisher R.C., Thorley-Lawson D.A.
      Mol. Cell. Biol. 11:1614-1623(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
    9. "TCT.1, a target molecule for gamma/delta T cells, is encoded by an immunoglobulin superfamily gene (Blast-1) located in the CD1 region of human chromosome 1."
      del Porto P., Mami-Chouaib F., Bruneau J.-M., Jitsukawa S., Dumas J., Harnois M., Hercend T.
      J. Exp. Med. 173:1339-1344(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
    10. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104; ASN-178 AND ASN-189.
      Tissue: Leukemic T-cell.
    11. "Solution structure of the first Ig-like domain from human CD48 antigen."
      RIKEN structural genomics initiative (RSGI)
      Submitted (AUG-2007) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 24-140.

    Entry informationi

    Entry nameiCD48_HUMAN
    AccessioniPrimary (citable) accession number: P09326
    Secondary accession number(s): Q5U055, Q8MGR0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 166 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3