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Protein

CD48 antigen

Gene

CD48

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

GO - Molecular functioni

GO - Biological processi

  • blood coagulation Source: Reactome
  • defense response Source: ProtInc
  • leukocyte migration Source: Reactome
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_12051. Cell surface interactions at the vascular wall.

Names & Taxonomyi

Protein namesi
Recommended name:
CD48 antigen
Alternative name(s):
B-lymphocyte activation marker BLAST-1
BCM1 surface antigen
Leukocyte antigen MEM-102
SLAM family member 2
Short name:
SLAMF2
Signaling lymphocytic activation molecule 2
TCT.1
CD_antigen: CD48
Gene namesi
Name:CD48
Synonyms:BCM1, BLAST1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:1683. CD48.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular exosome Source: UniProtKB
  • integral component of plasma membrane Source: ProtInc
  • membrane Source: UniProtKB
  • membrane raft Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26223.

Polymorphism and mutation databases

BioMutaiCD48.
DMDMi114871.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 220194CD48 antigenPRO_0000014881Add
BLAST
Propeptidei221 – 24323Removed in mature formBy similarityPRO_0000014882Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi104 – 1041N-linked (GlcNAc...)1 Publication
Disulfide bondi154 ↔ 196PROSITE-ProRule annotation
Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi178 – 1781N-linked (GlcNAc...); atypical1 Publication
Glycosylationi189 – 1891N-linked (GlcNAc...)1 Publication
Lipidationi220 – 2201GPI-anchor amidated serineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP09326.
PaxDbiP09326.
PeptideAtlasiP09326.
PRIDEiP09326.

PTM databases

PhosphoSiteiP09326.

Expressioni

Gene expression databases

BgeeiP09326.
CleanExiHS_CD48.
ExpressionAtlasiP09326. baseline and differential.
GenevisibleiP09326. HS.

Organism-specific databases

HPAiCAB002497.

Interactioni

Subunit structurei

Interacts with CD244 in an heterophilic manner.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CD244Q9BZW82EBI-714770,EBI-1580565

Protein-protein interaction databases

BioGridi107400. 5 interactions.
IntActiP09326. 4 interactions.
MINTiMINT-1427578.
STRINGi9606.ENSP00000357025.

Structurei

Secondary structure

1
243
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 363Combined sources
Beta strandi41 – 433Combined sources
Beta strandi51 – 6111Combined sources
Beta strandi64 – 696Combined sources
Beta strandi74 – 763Combined sources
Turni80 – 845Combined sources
Turni89 – 913Combined sources
Beta strandi94 – 985Combined sources
Helixi101 – 1033Combined sources
Beta strandi105 – 1139Combined sources
Turni114 – 1163Combined sources
Beta strandi117 – 1204Combined sources
Beta strandi126 – 1283Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EDONMR-A27-140[»]
ProteinModelPortaliP09326.
SMRiP09326. Positions 29-140.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09326.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 12799Ig-like C2-type 1Add
BLAST
Domaini132 – 21281Ig-like C2-type 2Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG40265.
GeneTreeiENSGT00510000049275.
HOGENOMiHOG000111487.
HOVERGENiHBG004490.
InParanoidiP09326.
KOiK06479.
OMAiAWIATWL.
OrthoDBiEOG7JQBPS.
PhylomeDBiP09326.
TreeFamiTF334964.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR015631. SLAM_fam_rcpts.
[Graphical view]
PANTHERiPTHR12080. PTHR12080. 1 hit.
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P09326-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCSRGWDSCL ALELLLLPLS LLVTSIQGHL VHMTVVSGSN VTLNISESLP
60 70 80 90 100
ENYKQLTWFY TFDQKIVEWD SRKSKYFESK FKGRVRLDPQ SGALYISKVQ
110 120 130 140 150
KEDNSTYIMR VLKKTGNEQE WKIKLQVLDP VPKPVIKIEK IEDMDDNCYL
160 170 180 190 200
KLSCVIPGES VNYTWYGDKR PFPKELQNSV LETTLMPHNY SRCYTCQVSN
210 220 230 240
SVSSKNGTVC LSPPCTLARS FGVEWIASWL VVTVPTILGL LLT
Length:243
Mass (Da):27,683
Last modified:November 1, 1991 - v2
Checksum:i9E46D76FC36A512C
GO
Isoform 2 (identifier: P09326-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     129-169: DPVPKPVIKI...SVNYTWYGDK → GESGEPKSKS...EEERKTSGQV
     170-243: Missing.

Note: No experimental confirmation available.
Show »
Length:169
Mass (Da):19,429
Checksum:i0E528B574BD04C5B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21C → W in AAA62834 (PubMed:1999351).Curated
Sequence conflicti66 – 661I → N in CAA29647 (PubMed:2828034).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti102 – 1021E → Q.
Corresponds to variant rs2295615 [ dbSNP | Ensembl ].
VAR_020082
Natural varianti241 – 2411L → S.
Corresponds to variant rs16832307 [ dbSNP | Ensembl ].
VAR_049909

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei129 – 16941DPVPK…WYGDK → GESGEPKSKSPLQWPQMDHC RASWEAWGTLGEEERKTSGQ V in isoform 2. 1 PublicationVSP_055598Add
BLAST
Alternative sequencei170 – 24374Missing in isoform 2. 1 PublicationVSP_055599Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59904 mRNA. Translation: AAA62834.1.
X06341 mRNA. Translation: CAA29647.1.
M37766 mRNA. Translation: AAA36211.1.
BT019813 mRNA. Translation: AAV38616.1.
AL121985 Genomic DNA. Translation: CAC00578.1.
CH471121 Genomic DNA. Translation: EAW52705.1.
BC016182 mRNA. Translation: AAH16182.1.
BC030224 mRNA. Translation: AAH30224.1.
M63911 Genomic DNA. Translation: AAA35602.1.
CCDSiCCDS1208.1. [P09326-1]
PIRiA53244.
RefSeqiNP_001769.2. NM_001778.3. [P09326-1]
UniGeneiHs.243564.

Genome annotation databases

EnsembliENST00000368045; ENSP00000357024; ENSG00000117091. [P09326-2]
ENST00000368046; ENSP00000357025; ENSG00000117091. [P09326-1]
GeneIDi962.
KEGGihsa:962.
UCSCiuc001fwn.4. human. [P09326-1]
uc001fwp.4. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59904 mRNA. Translation: AAA62834.1.
X06341 mRNA. Translation: CAA29647.1.
M37766 mRNA. Translation: AAA36211.1.
BT019813 mRNA. Translation: AAV38616.1.
AL121985 Genomic DNA. Translation: CAC00578.1.
CH471121 Genomic DNA. Translation: EAW52705.1.
BC016182 mRNA. Translation: AAH16182.1.
BC030224 mRNA. Translation: AAH30224.1.
M63911 Genomic DNA. Translation: AAA35602.1.
CCDSiCCDS1208.1. [P09326-1]
PIRiA53244.
RefSeqiNP_001769.2. NM_001778.3. [P09326-1]
UniGeneiHs.243564.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EDONMR-A27-140[»]
ProteinModelPortaliP09326.
SMRiP09326. Positions 29-140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107400. 5 interactions.
IntActiP09326. 4 interactions.
MINTiMINT-1427578.
STRINGi9606.ENSP00000357025.

PTM databases

PhosphoSiteiP09326.

Polymorphism and mutation databases

BioMutaiCD48.
DMDMi114871.

Proteomic databases

MaxQBiP09326.
PaxDbiP09326.
PeptideAtlasiP09326.
PRIDEiP09326.

Protocols and materials databases

DNASUi962.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368045; ENSP00000357024; ENSG00000117091. [P09326-2]
ENST00000368046; ENSP00000357025; ENSG00000117091. [P09326-1]
GeneIDi962.
KEGGihsa:962.
UCSCiuc001fwn.4. human. [P09326-1]
uc001fwp.4. human.

Organism-specific databases

CTDi962.
GeneCardsiGC01M160648.
HGNCiHGNC:1683. CD48.
HPAiCAB002497.
MIMi109530. gene.
neXtProtiNX_P09326.
PharmGKBiPA26223.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG40265.
GeneTreeiENSGT00510000049275.
HOGENOMiHOG000111487.
HOVERGENiHBG004490.
InParanoidiP09326.
KOiK06479.
OMAiAWIATWL.
OrthoDBiEOG7JQBPS.
PhylomeDBiP09326.
TreeFamiTF334964.

Enzyme and pathway databases

ReactomeiREACT_12051. Cell surface interactions at the vascular wall.

Miscellaneous databases

EvolutionaryTraceiP09326.
GeneWikiiCD48.
GenomeRNAii962.
NextBioi4020.
PROiP09326.
SOURCEiSearch...

Gene expression databases

BgeeiP09326.
CleanExiHS_CD48.
ExpressionAtlasiP09326. baseline and differential.
GenevisibleiP09326. HS.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR015631. SLAM_fam_rcpts.
[Graphical view]
PANTHERiPTHR12080. PTHR12080. 1 hit.
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1."
    Korinek V., Stefanova I., Angelisova P., Hilbert I., Horejsi V.
    Immunogenetics 33:108-112(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Molecular cloning of the lymphocyte activation marker Blast-1."
    Staunton D.E., Thorley-Lawson D.A.
    EMBO J. 6:3695-3701(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: B-cell and Pancreas.
  8. "Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48)."
    Fisher R.C., Thorley-Lawson D.A.
    Mol. Cell. Biol. 11:1614-1623(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
  9. "TCT.1, a target molecule for gamma/delta T cells, is encoded by an immunoglobulin superfamily gene (Blast-1) located in the CD1 region of human chromosome 1."
    del Porto P., Mami-Chouaib F., Bruneau J.-M., Jitsukawa S., Dumas J., Harnois M., Hercend T.
    J. Exp. Med. 173:1339-1344(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
  10. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104; ASN-178 AND ASN-189.
    Tissue: Leukemic T-cell.
  11. "Solution structure of the first Ig-like domain from human CD48 antigen."
    RIKEN structural genomics initiative (RSGI)
    Submitted (AUG-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 24-140.

Entry informationi

Entry nameiCD48_HUMAN
AccessioniPrimary (citable) accession number: P09326
Secondary accession number(s): Q5U055, Q8MGR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1991
Last modified: June 24, 2015
This is version 173 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.