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P09326 (CD48_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 164. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD48 antigen
Alternative name(s):
B-lymphocyte activation marker BLAST-1
BCM1 surface antigen
Leukocyte antigen MEM-102
SLAM family member 2
Short name=SLAMF2
Signaling lymphocytic activation molecule 2
TCT.1
CD_antigen=CD48
Gene names
Name:CD48
Synonyms:BCM1, BLAST1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Subunit structure

Interacts with CD244 in an heterophilic manner By similarity.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor By similarity.

Sequence similarities

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainImmunoglobulin domain
Repeat
Signal
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processT cell activation

Inferred from electronic annotation. Source: Ensembl

blood coagulation

Traceable author statement. Source: Reactome

defense response

Traceable author statement Ref.3. Source: ProtInc

leukocyte migration

Traceable author statement. Source: Reactome

mast cell activation

Inferred from electronic annotation. Source: Ensembl

signal transduction

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentanchored component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

external side of plasma membrane

Inferred from electronic annotation. Source: Ensembl

extracellular vesicular exosome

Inferred from direct assay PubMed 20458337. Source: UniProt

integral component of plasma membrane

Traceable author statement PubMed 1693656. Source: ProtInc

membrane raft

Inferred from direct assay PubMed 11313396. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

protein complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionantigen binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 12356317. Source: UniProtKB

receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CD244Q9BZW82EBI-714770,EBI-1580565

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 220194CD48 antigen
PRO_0000014881
Propeptide221 – 24323Removed in mature form By similarity
PRO_0000014882

Regions

Domain29 – 12799Ig-like C2-type 1
Domain132 – 21281Ig-like C2-type 2

Amino acid modifications

Lipidation2201GPI-anchor amidated serine By similarity
Glycosylation401N-linked (GlcNAc...) Potential
Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation1041N-linked (GlcNAc...) Ref.10
Glycosylation1621N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...); atypical Ref.10
Glycosylation1891N-linked (GlcNAc...) Ref.10
Disulfide bond154 ↔ 196 Potential

Natural variations

Natural variant1021E → Q.
Corresponds to variant rs2295615 [ dbSNP | Ensembl ].
VAR_020082
Natural variant2411L → S.
Corresponds to variant rs16832307 [ dbSNP | Ensembl ].
VAR_049909

Experimental info

Sequence conflict21C → W in AAA62834. Ref.1
Sequence conflict661I → N in CAA29647. Ref.3

Secondary structure

......................... 243
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P09326 [UniParc].

Last modified November 1, 1991. Version 2.
Checksum: 9E46D76FC36A512C

FASTA24327,683
        10         20         30         40         50         60 
MCSRGWDSCL ALELLLLPLS LLVTSIQGHL VHMTVVSGSN VTLNISESLP ENYKQLTWFY 

        70         80         90        100        110        120 
TFDQKIVEWD SRKSKYFESK FKGRVRLDPQ SGALYISKVQ KEDNSTYIMR VLKKTGNEQE 

       130        140        150        160        170        180 
WKIKLQVLDP VPKPVIKIEK IEDMDDNCYL KLSCVIPGES VNYTWYGDKR PFPKELQNSV 

       190        200        210        220        230        240 
LETTLMPHNY SRCYTCQVSN SVSSKNGTVC LSPPCTLARS FGVEWIASWL VVTVPTILGL 


LLT 

« Hide

References

« Hide 'large scale' references
[1]"The isolation of cDNA clones for CD48."
Vaughan H.A., Henning M.M., Purcell D.F.J., McKenzie I.F.C., Sandrin M.S.
Immunogenetics 33:113-117(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The human leucocyte antigen CD48 (MEM-102) is closely related to the activation marker Blast-1."
Korinek V., Stefanova I., Angelisova P., Hilbert I., Horejsi V.
Immunogenetics 33:108-112(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Molecular cloning of the lymphocyte activation marker Blast-1."
Staunton D.E., Thorley-Lawson D.A.
EMBO J. 6:3695-3701(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: B-cell.
[8]"Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48)."
Fisher R.C., Thorley-Lawson D.A.
Mol. Cell. Biol. 11:1614-1623(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-27.
[9]"TCT.1, a target molecule for gamma/delta T cells, is encoded by an immunoglobulin superfamily gene (Blast-1) located in the CD1 region of human chromosome 1."
del Porto P., Mami-Chouaib F., Bruneau J.-M., Jitsukawa S., Dumas J., Harnois M., Hercend T.
J. Exp. Med. 173:1339-1344(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
[10]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104; ASN-178 AND ASN-189.
Tissue: Leukemic T-cell.
[11]"Solution structure of the first Ig-like domain from human CD48 antigen."
RIKEN structural genomics initiative (RSGI)
Submitted (AUG-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 24-140.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59904 mRNA. Translation: AAA62834.1.
X06341 mRNA. Translation: CAA29647.1.
M37766 mRNA. Translation: AAA36211.1.
BT019813 mRNA. Translation: AAV38616.1.
AL121985 Genomic DNA. Translation: CAC00578.1.
CH471121 Genomic DNA. Translation: EAW52705.1.
BC016182 mRNA. Translation: AAH16182.1.
M63911 Genomic DNA. Translation: AAA35602.1.
CCDSCCDS1208.1.
PIRA53244.
RefSeqNP_001769.2. NM_001778.3.
UniGeneHs.243564.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EDONMR-A27-140[»]
ProteinModelPortalP09326.
SMRP09326. Positions 29-140.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107400. 4 interactions.
IntActP09326. 4 interactions.
MINTMINT-1427578.
STRING9606.ENSP00000357025.

PTM databases

PhosphoSiteP09326.

Polymorphism databases

DMDM114871.

Proteomic databases

MaxQBP09326.
PaxDbP09326.
PeptideAtlasP09326.
PRIDEP09326.

Protocols and materials databases

DNASU962.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000368046; ENSP00000357025; ENSG00000117091.
GeneID962.
KEGGhsa:962.
UCSCuc001fwn.4. human.

Organism-specific databases

CTD962.
GeneCardsGC01M160648.
HGNCHGNC:1683. CD48.
HPACAB002497.
MIM109530. gene.
neXtProtNX_P09326.
PharmGKBPA26223.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40265.
HOGENOMHOG000111487.
HOVERGENHBG004490.
InParanoidP09326.
KOK06479.
OMAAWIATWL.
OrthoDBEOG7JQBPS.
PhylomeDBP09326.
TreeFamTF334964.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.

Gene expression databases

ArrayExpressP09326.
BgeeP09326.
CleanExHS_CD48.
GenevestigatorP09326.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR015631. SLAM_fam_rcpts.
[Graphical view]
PANTHERPTHR12080. PTHR12080. 1 hit.
PfamPF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP09326.
GeneWikiCD48.
GenomeRNAi962.
NextBio4020.
PROP09326.
SOURCESearch...

Entry information

Entry nameCD48_HUMAN
AccessionPrimary (citable) accession number: P09326
Secondary accession number(s): Q5U055
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1991
Last modified: July 9, 2014
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries