PTS system N-acetylglucosamine-specific EIICBA component

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P09323 (PTW3C_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
PTS system N-acetylglucosamine-specific EIICBA component

Alternative name(s):
EIICBA-Nag
Short name=EII-Nag

Including the following 3 domains:

N-acetylglucosamine permease IIC component
Alternative name(s):
PTS system N-acetylglucosamine-specific EIIC component
N-acetylglucosamine-specific phosphotransferase enzyme IIB component
EC=2.7.1.69
Alternative name(s):
PTS system N-acetylglucosamine-specific EIIB component
N-acetylglucosamine-specific phosphotransferase enzyme IIA component
EC=2.7.1.-
Alternative name(s):
PTS system N-acetylglucosamine-specific EIIA component

Gene names

Name:	nagE
Synonyms:	pstN
Ordered Locus Names:	b0679, JW0665

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N-acetylglucosamine transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.6.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Non-traceable author statement. Source: UniProtKB organelle inner membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylglucosamine transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro transferase activity Non-traceable author statement. Source: UniProtKB transporter activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 648

648

PTS system N-acetylglucosamine-specific EIICBA component

PRO_0000186475

Regions

Transmembrane

16 – 36

Helical; Potential

Transmembrane

38 – 58

Helical; Potential

Transmembrane

70 – 90

Helical; Potential

Transmembrane

92 – 112

Helical; Potential

Transmembrane

132 – 152

Helical; Potential

Transmembrane

159 – 179

Helical; Potential

Transmembrane

192 – 212

Helical; Potential

Transmembrane

232 – 252

Helical; Potential

Transmembrane

260 – 280

Helical; Potential

Transmembrane

282 – 302

Helical; Potential

Transmembrane

303 – 323

Helical; Potential

Transmembrane

339 – 359

Helical; Potential

Domain

1 – 371

371

PTS EIIC type-1

Domain

390 – 472

PTS EIIB type-1

Domain

517 – 621

105

PTS EIIA type-1

Sites

Active site

412

Phosphocysteine intermediate; for EIIB activity By similarity

Active site

569

Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P09323 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 1E24C97CFCBBAA59
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FASTA

648

68,347

        10         20         30         40         50         60 
MNILGFFQRL GRALQLPIAV LPVAALLLRF GQPDLLNVAF IAQAGGAIFD NLALIFAIGV 

        70         80         90        100        110        120 
ASSWSKDSAG AAALAGAVGY FVLTKAMVTI NPEINMGVLA GIITGLVGGA AYNRWSDIKL 

       130        140        150        160        170        180 
PDFLSFFGGK RFVPIATGFF CLVLAAIFGY VWPPVQHAIH AGGEWIVSAG ALGSGIFGFI 

       190        200        210        220        230        240 
NRLLIPTGLH QVLNTIAWFQ IGEFTNAAGT VFHGDINRFY AGDGTAGMFM SGFFPIMMFG 

       250        260        270        280        290        300 
LPGAALAMYF AAPKERRPMV GGMLLSVAVT AFLTGVTEPL EFLFMFLAPL LYLLHALLTG 

       310        320        330        340        350        360 
ISLFVATLLG IHAGFSFSAG AIDYALMYNL PAASQNVWML LVMGVIFFAI YFVVFSLVIR 

       370        380        390        400        410        420 
MFNLKTPGRE DKEDEIVTEE ANSNTEEGLT QLATNYIAAV GGTDNLKAID ACITRLRLTV 

       430        440        450        460        470        480 
ADSARVNDTM CKRLGASGVV KLNKQTIQVI VGAKAESIGD AMKKVVARGP VAAASAEATP 

       490        500        510        520        530        540 
ATAAPVAKPQ AVPNAVSIAE LVSPITGDVV ALDQVPDEAF ASKAVGDGVA VKPTDKIVVS 

       550        560        570        580        590        600 
PAAGTIVKIF NTNHAFCLET EKGAEIVVHM GIDTVALEGK GFKRLVEEGA QVSAGQPILE 

       610        620        630        640 
MDLDYLNANA RSMISPVVCS NIDDFSGLII KAQGHIVAGQ TPLYEIKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase." Rogers M.J., Ohgi T., Plumbridge J., Soell D. Gene 62:197-207(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of cloned enzyme IIN-acetylglucosamine of the phosphoenolpyruvate:N-acetylglucosamine phosphotransferase system of Escherichia coli." Peri K.G., Waygood E.B. Biochemistry 27:6054-6061(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19284 Genomic DNA. Translation: AAA24192.1. U00096 Genomic DNA. Translation: AAC73773.1. AP009048 Genomic DNA. Translation: BAA35327.1.
PIR	WQEC2N. B29895.
RefSeq	NP_415205.1. NC_000913.2. YP_488959.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P09323.
SMR	P09323. Positions 392-466, 500-648.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b0679.
Protein family/group databases
TCDB	4.A.1.1.2. PTS glucose-glucoside (Glc) family.
Proteomic databases
PaxDb	P09323.
PRIDE	P09323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73773; AAC73773; b0679. BAA35327; BAA35327; BAA35327.
GeneID	12932215. 945292.
KEGG	ecj:Y75_p0658. eco:b0679.
PATRIC	32116545. VBIEscCol129921_0705.
Organism-specific databases
EchoBASE	EB0629.
EcoGene	EG10635. nagE.
Phylogenomic databases
eggNOG	COG1263.
HOGENOM	HOG000250993.
KO	K02802. K02803. K02804.
OMA	QANLEMI.
ProtClustDB	PRK10255.
Enzyme and pathway databases
BioCyc	EcoCyc:NAGE-MONOMER. ECOL316407:JW0665-MONOMER.
Gene expression databases
Genevestigator	P09323.
Family and domain databases
Gene3D	3.30.1360.60. 1 hit.
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR010974. PTS_IIBC_nag. [Graphical view]
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit. SSF55604. PTS_EIIB. 1 hit.
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit. TIGR01998. PTS-II-BC-nag. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTW3C_ECOLI

Accession

Primary (citable) accession number: P09323

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 3 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents