ID   G3PA_MAIZE              Reviewed;         403 AA.
AC   P09315; P15985;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   16-JUN-2009, entry version 77.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic;
DE            EC=1.2.1.13;
DE   AltName: Full=NADP-dependent glyceraldehydephosphate dehydrogenase subunit A;
DE   Flags: Precursor;
GN   Name=GAPA; Synonyms=GPA1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae;
OC   PACCAD clade; Panicoideae; Andropogoneae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=90133970; PubMed=2515291; DOI=10.1007/BF02602911;
RA   Quigley F., Brinkmann H., Martin W.F., Cerff R.;
RT   "Strong functional GC pressure in a light-regulated maize gene
RT   encoding subunit GAPA of chloroplast glyceraldehyde-3-phosphate
RT   dehydrogenase: implications for the evolution of GAPA pseudogenes.";
RL   J. Mol. Evol. 29:412-421(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=88190130; PubMed=3357887; DOI=10.1073/pnas.85.8.2672;
RA   Quigley F., Martin W.F., Cerff R.;
RT   "Intron conservation across the prokaryote-eukaryote boundary:
RT   structure of the nuclear gene for chloroplast glyceraldehyde-3-
RT   phosphate dehydrogenase from maize.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:2672-2676(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE OF 1-125 AND 301-403.
RC   STRAIN=cv. W64 X W128E; TISSUE=Leaf;
RX   PubMed=16666879;
RA   Gowri G., Campbell W.H.;
RT   "cDNA clones for corn leaf NADH: nitrate reductase and chloroplast
RT   NAD(P)(+): glyceraldehyde-3-phosphate dehydrogenase.";
RL   Plant Physiol. 90:792-798(1989).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NADP(+) = 3-phospho-D-glyceroyl phosphate + NADPH.
CC   -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC   -!- SUBUNIT: Tetramer of either four A chains (GAPDH 2) or two A and
CC       two B chains (GAPDH 1) (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; X15408; CAA33455.1; -; Genomic_DNA.
DR   EMBL; M18976; AAA33464.1; -; Genomic_DNA.
DR   EMBL; M31481; AAA33484.1; -; mRNA.
DR   EMBL; M31483; AAA33485.1; -; mRNA.
DR   PIR; A30890; DEZMG3.
DR   RefSeq; NP_001105414.1; -.
DR   UniGene; Zm.7; -.
DR   HSSP; P19866; 1NBO.
DR   SMR; P09315; 67-403.
DR   GeneID; 542368; -.
DR   Gramene; P09315; -.
DR   MaizeGDB; 13872; -.
DR   BRENDA; 1.2.1.13; 289.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0047100; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IEA:EC.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Calvin cycle; Chloroplast; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT       1     66       Chloroplast.
FT   CHAIN        67    403       Glyceraldehyde-3-phosphate dehydrogenase
FT                                A, chloroplastic.
FT                                /FTId=PRO_0000010422.
FT   NP_BIND      77     78       NADP (By similarity).
FT   REGION      219    221       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      278    279       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    220    220       Nucleophile (By similarity).
FT   BINDING     102    102       NADP (By similarity).
FT   BINDING     147    147       NADP; via carbonyl oxygen (By
FT                                similarity).
FT   BINDING     250    250       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     265    265       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     301    301       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     383    383       NADP (By similarity).
FT   SITE        247    247       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   403 AA;  42867 MW;  0505124C3F458DD8 CRC64;
     MASSMLSATT VPLQQGGGLS EFSGLRSSAS LPMRRNATSD DFMSAVSFRT HAVGTSGGPR
     RAPTEAKLKV AINGFGRIGR NFLRCWHGRG DASPLDVIAI NDTGGVKQAS HLLKYDSTLG
     IFDADVKPVG DNAISVDGKV IKVVSDRNPS NLPWGELGID LVIEGTGVFV DREGAGKHIQ
     AGAKKVLITA PGKGDIPTYV VGVNADQYNP DEPIISNASC TTNCLAPFVK VLDQKFGIIK
     GTMTTTHSYT GDQRLLDASH RDLRRARAAA LNIVPTSTGA AKAVSLVLPN LKGKLNGIAL
     RVPTPNVSVV DLVVQVSKKT LAEEVNQAFR DAAANELTGI LEVCDVPLVS VDFRCSDVSS
     TIDASLTMVM GDDMVKVISW YDNEWGYSQR VVDLADICAN QWK
//