Reviewed, UniProtKB/Swiss-Prot P09315 (G3PA_MAIZE)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit A

Gene names

Name:	GAPA
Synonyms:	GPA1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.
Subcellular location	Plastid › chloroplast.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 66	66	Chloroplast
	Chain	67 – 403	337	Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic		PRO_0000010422
Regions
	Nucleotide binding	77 – 78	2	NADP By similarity
	Region	219 – 221	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	278 – 279	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	220	1	Nucleophile By similarity
	Binding site	102	1	NADP By similarity
	Binding site	147	1	NADP; via carbonyl oxygen By similarity
	Binding site	250	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	265	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	301	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	383	1	NADP By similarity
	Site	247	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09315-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 0505124C3F458DD8	FASTA	403	42,867
	10 20 30 40 50 60 MASSMLSATT VPLQQGGGLS EFSGLRSSAS LPMRRNATSD DFMSAVSFRT HAVGTSGGPR 70 80 90 100 110 120 RAPTEAKLKV AINGFGRIGR NFLRCWHGRG DASPLDVIAI NDTGGVKQAS HLLKYDSTLG 130 140 150 160 170 180 IFDADVKPVG DNAISVDGKV IKVVSDRNPS NLPWGELGID LVIEGTGVFV DREGAGKHIQ 190 200 210 220 230 240 AGAKKVLITA PGKGDIPTYV VGVNADQYNP DEPIISNASC TTNCLAPFVK VLDQKFGIIK 250 260 270 280 290 300 GTMTTTHSYT GDQRLLDASH RDLRRARAAA LNIVPTSTGA AKAVSLVLPN LKGKLNGIAL 310 320 330 340 350 360 RVPTPNVSVV DLVVQVSKKT LAEEVNQAFR DAAANELTGI LEVCDVPLVS VDFRCSDVSS 370 380 390 400 TIDASLTMVM GDDMVKVISW YDNEWGYSQR VVDLADICAN QWK

References

[1]	"Strong functional GC pressure in a light-regulated maize gene encoding subunit GAPA of chloroplast glyceraldehyde-3-phosphate dehydrogenase: implications for the evolution of GAPA pseudogenes." Quigley F., Brinkmann H., Martin W.F., Cerff R. J. Mol. Evol. 29:412-421(1989) [PubMed: 2515291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Intron conservation across the prokaryote-eukaryote boundary: structure of the nuclear gene for chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize." Quigley F., Martin W.F., Cerff R. Proc. Natl. Acad. Sci. U.S.A. 85:2672-2676(1988) [PubMed: 3357887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"cDNA clones for corn leaf NADH: nitrate reductase and chloroplast NAD(P)(+): glyceraldehyde-3-phosphate dehydrogenase." Gowri G., Campbell W.H. Plant Physiol. 90:792-798(1989) [PubMed: 16666879] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-125 AND 301-403. Strain: cv. W64 X W128E. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15408 Genomic DNA. Translation: CAA33455.1. M18976 Genomic DNA. Translation: AAA33464.1. M31481 mRNA. Translation: AAA33484.1. M31483 mRNA. Translation: AAA33485.1.
PIR	DEZMG3. A30890.
RefSeq	NP_001105414.1.
UniGene	Zm.7
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P09315. Positions 67-403.
ModBase	Search...
Genome annotation databases
GeneID	542368.
Organism-specific databases
Gramene	P09315.
MaizeGDB	13872.
Enzyme and pathway databases
BRENDA	1.2.1.13. 289.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PA_MAIZE

Accession

Primary (citable) accession number: P09315
Secondary accession number(s): P15985

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)