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Protein

Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic

Gene

GAPA

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102NADPBy similarity1
Binding sitei147NADP; via carbonyl oxygenBy similarity1
Active sitei220NucleophilePROSITE-ProRule annotation1
Sitei247Activates thiol group during catalysisBy similarity1
Binding sitei250Glyceraldehyde 3-phosphateBy similarity1
Binding sitei265Glyceraldehyde 3-phosphateBy similarity1
Binding sitei301Glyceraldehyde 3-phosphateBy similarity1
Binding sitei383NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 78NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • glucose metabolic process Source: InterPro
  • reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  • response to light stimulus Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit A
Gene namesi
Name:GAPA
Synonyms:GPA1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Organism-specific databases

MaizeGDBi13872.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 66ChloroplastAdd BLAST66
ChainiPRO_000001042267 – 403Glyceraldehyde-3-phosphate dehydrogenase A, chloroplasticAdd BLAST337

Proteomic databases

PRIDEiP09315.

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).By similarity

Structurei

3D structure databases

ProteinModelPortaliP09315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 221Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni278 – 279Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000071679.
KOiK05298.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSMLSATT VPLQQGGGLS EFSGLRSSAS LPMRRNATSD DFMSAVSFRT
60 70 80 90 100
HAVGTSGGPR RAPTEAKLKV AINGFGRIGR NFLRCWHGRG DASPLDVIAI
110 120 130 140 150
NDTGGVKQAS HLLKYDSTLG IFDADVKPVG DNAISVDGKV IKVVSDRNPS
160 170 180 190 200
NLPWGELGID LVIEGTGVFV DREGAGKHIQ AGAKKVLITA PGKGDIPTYV
210 220 230 240 250
VGVNADQYNP DEPIISNASC TTNCLAPFVK VLDQKFGIIK GTMTTTHSYT
260 270 280 290 300
GDQRLLDASH RDLRRARAAA LNIVPTSTGA AKAVSLVLPN LKGKLNGIAL
310 320 330 340 350
RVPTPNVSVV DLVVQVSKKT LAEEVNQAFR DAAANELTGI LEVCDVPLVS
360 370 380 390 400
VDFRCSDVSS TIDASLTMVM GDDMVKVISW YDNEWGYSQR VVDLADICAN

QWK
Length:403
Mass (Da):42,867
Last modified:July 1, 1989 - v1
Checksum:i0505124C3F458DD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15408 Genomic DNA. Translation: CAA33455.1.
M18976 Genomic DNA. Translation: AAA33464.1.
M31481 mRNA. Translation: AAA33484.1.
M31483 mRNA. Translation: AAA33485.1.
PIRiA30890. DEZMG3.
RefSeqiNP_001105414.1. NM_001111944.1.
UniGeneiZm.7.

Genome annotation databases

GeneIDi542368.
KEGGizma:542368.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15408 Genomic DNA. Translation: CAA33455.1.
M18976 Genomic DNA. Translation: AAA33464.1.
M31481 mRNA. Translation: AAA33484.1.
M31483 mRNA. Translation: AAA33485.1.
PIRiA30890. DEZMG3.
RefSeqiNP_001105414.1. NM_001111944.1.
UniGeneiZm.7.

3D structure databases

ProteinModelPortaliP09315.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP09315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi542368.
KEGGizma:542368.

Organism-specific databases

MaizeGDBi13872.

Phylogenomic databases

HOGENOMiHOG000071679.
KOiK05298.

Enzyme and pathway databases

UniPathwayiUPA00116.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PA_MAIZE
AccessioniPrimary (citable) accession number: P09315
Secondary accession number(s): P15985
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 5, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants contain two types of GAPDH: cytosolic forms which participate in glycolysis and chloroplast forms which participate in photosynthesis. All the forms are encoded by distinct genes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.