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Protein

Major viral transcription factor ICP4 homolog

Gene

ORF62

more
Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional transactivator. May interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. Negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis.1 Publication

Miscellaneous

Encoded both by ORF62 and ORF71 within the inverted repeat sequences bounding the Us region of the VZV genome.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Major viral transcription factor ICP4 homolog
Alternative name(s):
Immediate-early protein 62
Short name:
IE62
Gene namesi
ORF Names:ORF62
AND
ORF Names:ORF71
OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri10338 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000002602 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi545Q → L: Decreases DNA-binding. 1 Publication1
Mutagenesisi546N → L: Decreases DNA-binding. 1 Publication1
Mutagenesisi548K → E: Complete loss of DNA-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001158201 – 1310Major viral transcription factor ICP4 homologAdd BLAST1310

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei686Phosphoserine; by VZV ORF661 Publication1
Modified residuei722Phosphoserine; by VZV ORF661 Publication1

Post-translational modificationi

Phosphorylated by ORF66 protein kinase on Ser-686 and Ser-722. Also phosphorylated by ORF47 protein kinase and by human CSNK2A1/CKII.2 Publications

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP09310.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Interacts with IE4 and IE63. Interacts with host USF1 and SP1.4 Publications

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi677 – 685Nuclear localization signalSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi353 – 365Ser-richAdd BLAST13
Compositional biasi1285 – 1290Poly-Ser6

Sequence similaritiesi

Belongs to the herpesviridae ICP4 family.Curated

Phylogenomic databases

OrthoDBiVOG09000011.

Family and domain databases

InterProiView protein in InterPro
IPR005205. Herpes_ICP4_C.
IPR005206. Herpes_ICP4_N.
PfamiView protein in Pfam
PF03585. Herpes_ICP4_C. 1 hit.
PF03584. Herpes_ICP4_N. 1 hit.

Sequencei

Sequence statusi: Complete.

P09310-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTPPMQRST PQRAGSPDTL ELMDLLDAAA AAAEHRARVV TSSQPDDLLF
60 70 80 90 100
GENGVMVGRE HEIVSIPSVS GLQPEPRTED VGEELTQDDY VCEDGQDLMG
110 120 130 140 150
SPVIPLAEVF HTRFSEAGAR EPTGADRSLE TVSLGTKLAR SPKPPMNDGE
160 170 180 190 200
TGRGTTPPFP QAFSPVSPAS PVGDAAGNDQ REDQRSIPRQ TTRGNSPGLP
210 220 230 240 250
SVVHRDRQTQ SISGKKPGDE QAGHAHASGD GVVLQKTQRP AQGKSPKKKT
260 270 280 290 300
LKVKVPLPAR KPGGPVPGPV EQLYHVLSDS VPAKGAKADL PFETDDTRPR
310 320 330 340 350
KHDARGITPR VPGRSSGGKP RAFLALPGRS HAPDPIEDDS PVEKKPKSRE
360 370 380 390 400
FVSSSSSSSS WGSSSEDEDD EPRRVSVGSE TTGSRSGREH APSPSNSDDS
410 420 430 440 450
DSNDGGSTKQ NIQPGYRSIS GPDPRIRKTK RLAGEPGRQR QKSFSLPRSR
460 470 480 490 500
TPIIPPVSGP LMMPDGSPWP GSAPLPSNRV RFGPSGETRE GHWEDEAVRA
510 520 530 540 550
ARARYEASTE PVPLYVPELG DPARQYRALI NLIYCPDRDP IAWLQNPKLT
560 570 580 590 600
GVNSALNQFY QKLLPPGRAG TAVTGSVASP VPHVGEAMAT GEALWALPHA
610 620 630 640 650
AAAVAMSRRY DRAQKHFILQ SLRRAFASMA YPEATGSSPA ARISRGHPSP
660 670 680 690 700
TTPATQAPDP QPSAAARSLS VCPPDDRLRT PRKRKSQPVE SRSLLDKIRE
710 720 730 740 750
TPVADARVAD DHVVSKAKRR VSEPVTITSG PVVDPPAVIT MPLDGPAPNG
760 770 780 790 800
GFRRIPRGAL HTPVPSDQAR KAYCTPETIA RLVDDPLFPT AWRPALSFDP
810 820 830 840 850
GALAEIAARR PGGGDRRFGP PSGVEALRRR CAWMRQIPDP EDVRLLIIYD
860 870 880 890 900
PLPGEDINGP LESTLATDPG PSWSPSRGGL SVVLAALSNR LCLPSTHAWA
910 920 930 940 950
GNWTGPPDVS ALNARGVLLL STRDLAFAGA VEYLGSRLAS ARRRLLVLDA
960 970 980 990 1000
VALERWPRDG PALSQYHVYV RAPARPDAQA VVRWPDSAVT EGLARAVFAS
1010 1020 1030 1040 1050
SRTFGPASFA RIETAFANLY PGEQPLCLCR GGNVAYTVCT RAGPKTRVPL
1060 1070 1080 1090 1100
SPREYRQYVL PGFDGCKDLA RQSRGLGLGA ADFVDEAAHS HRAANRWGLG
1110 1120 1130 1140 1150
AALRPVFLPE GRRPGAAGPE AGDVPTWARV FCRHALLEPD PAAEPLVLPP
1160 1170 1180 1190 1200
VAGRSVALYA SADEARNALP PIPRVMWPPG FGAAETVLEG SDGTRFVFGH
1210 1220 1230 1240 1250
HGGSERPSET QAGRQRRTAD DREHALELDD WEVGCEDAWD SEEGGGDDGD
1260 1270 1280 1290 1300
APGSSFGVSI VSVAPGVLRD RRVGLRPAVK VELLSSSSSS EDEDDVWGGR
1310
GGRSPPQSRG
Length:1,310
Mass (Da):139,998
Last modified:July 1, 1989 - v1
Checksum:iAF58321D95CD9B56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27954.1.
X04370 Genomic DNA. Translation: CAA27945.1.
X02132 Genomic DNA. Translation: CAA26044.1.
PIRiA27345. WZBE62.
RefSeqiNP_040184.1. NC_001348.1.
NP_040193.1. NC_001348.1.

Genome annotation databases

GeneIDi1487695.
1487699.
KEGGivg:1487695.
vg:1487699.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiICP4_VZVD
AccessioniPrimary (citable) accession number: P09310
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 5, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families