Reviewed, UniProtKB/Swiss-Prot P09310 (ICP4_VZVD)

Names and origin

Protein names

Recommended name:
    Transcriptional transactivator IE62
Alternative name(s):
    Immediate-early protein 62
      Short name=IE62

Gene names

ORF Names:	ORF62
AND
ORF Names:	ORF71

Organism

Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)

Taxonomic identifier

10338 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	1310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional transactivator. May interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. Negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis. Ref.4
Subunit structure	Interacts with IE4 and IE63. Interacts with host USF1 and SP1. Ref.6 Ref.10 Ref.11 Ref.12
Subcellular location	Host nucleus By similarity. Host cytoplasm By similarity. Virion tegument By similarity. Note: Localizes to the cytoplasm when phosphorylated.
Post-translational modification	Phosphorylated by ORF66 protein kinase on Ser-686 and Ser-722. Also phosphorylated by ORF47 protein kinase and by human CSNK2A1/CKII. Ref.9 Ref.13
Miscellaneous	Encoded both by ORF62 and ORF71 within the inverted repeat sequences bounding the Us region of the VZV genome.
Sequence similarities	Belongs to the herpesviridae ICP4/IE140/IE180 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Host cytoplasm Host nucleus Virion Virion tegument
Developmental stage	Early protein
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Virus reference strain
Gene Ontology (GO)
Biological process	positive regulation of transcription Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-KW viral tegument Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription activator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1310	1310	Transcriptional transactivator IE62		PRO_0000115820
Regions
	Motif	677 – 685	9	Nuclear localization signal Potential
	Compositional bias	353 – 365	13	Ser-rich
	Compositional bias	1285 – 1290	6	Poly-Ser
Amino acid modifications
	Modified residue	686	1	Phosphoserine; by VZV ORF66 Ref.13
	Modified residue	722	1	Phosphoserine; by VZV ORF66 Ref.13
Experimental info
	Mutagenesis	545	1	Q → L: Decreases DNA-binding. Ref.5
	Mutagenesis	546	1	N → L: Decreases DNA-binding. Ref.5
	Mutagenesis	548	1	K → E: Complete loss of DNA-binding. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P09310-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: AF58321D95CD9B56	FASTA	1,310	139,998
	10 20 30 40 50 60 MDTPPMQRST PQRAGSPDTL ELMDLLDAAA AAAEHRARVV TSSQPDDLLF GENGVMVGRE 70 80 90 100 110 120 HEIVSIPSVS GLQPEPRTED VGEELTQDDY VCEDGQDLMG SPVIPLAEVF HTRFSEAGAR 130 140 150 160 170 180 EPTGADRSLE TVSLGTKLAR SPKPPMNDGE TGRGTTPPFP QAFSPVSPAS PVGDAAGNDQ 190 200 210 220 230 240 REDQRSIPRQ TTRGNSPGLP SVVHRDRQTQ SISGKKPGDE QAGHAHASGD GVVLQKTQRP 250 260 270 280 290 300 AQGKSPKKKT LKVKVPLPAR KPGGPVPGPV EQLYHVLSDS VPAKGAKADL PFETDDTRPR 310 320 330 340 350 360 KHDARGITPR VPGRSSGGKP RAFLALPGRS HAPDPIEDDS PVEKKPKSRE FVSSSSSSSS 370 380 390 400 410 420 WGSSSEDEDD EPRRVSVGSE TTGSRSGREH APSPSNSDDS DSNDGGSTKQ NIQPGYRSIS 430 440 450 460 470 480 GPDPRIRKTK RLAGEPGRQR QKSFSLPRSR TPIIPPVSGP LMMPDGSPWP GSAPLPSNRV 490 500 510 520 530 540 RFGPSGETRE GHWEDEAVRA ARARYEASTE PVPLYVPELG DPARQYRALI NLIYCPDRDP 550 560 570 580 590 600 IAWLQNPKLT GVNSALNQFY QKLLPPGRAG TAVTGSVASP VPHVGEAMAT GEALWALPHA 610 620 630 640 650 660 AAAVAMSRRY DRAQKHFILQ SLRRAFASMA YPEATGSSPA ARISRGHPSP TTPATQAPDP 670 680 690 700 710 720 QPSAAARSLS VCPPDDRLRT PRKRKSQPVE SRSLLDKIRE TPVADARVAD DHVVSKAKRR 730 740 750 760 770 780 VSEPVTITSG PVVDPPAVIT MPLDGPAPNG GFRRIPRGAL HTPVPSDQAR KAYCTPETIA 790 800 810 820 830 840 RLVDDPLFPT AWRPALSFDP GALAEIAARR PGGGDRRFGP PSGVEALRRR CAWMRQIPDP 850 860 870 880 890 900 EDVRLLIIYD PLPGEDINGP LESTLATDPG PSWSPSRGGL SVVLAALSNR LCLPSTHAWA 910 920 930 940 950 960 GNWTGPPDVS ALNARGVLLL STRDLAFAGA VEYLGSRLAS ARRRLLVLDA VALERWPRDG 970 980 990 1000 1010 1020 PALSQYHVYV RAPARPDAQA VVRWPDSAVT EGLARAVFAS SRTFGPASFA RIETAFANLY 1030 1040 1050 1060 1070 1080 PGEQPLCLCR GGNVAYTVCT RAGPKTRVPL SPREYRQYVL PGFDGCKDLA RQSRGLGLGA 1090 1100 1110 1120 1130 1140 ADFVDEAAHS HRAANRWGLG AALRPVFLPE GRRPGAAGPE AGDVPTWARV FCRHALLEPD 1150 1160 1170 1180 1190 1200 PAAEPLVLPP VAGRSVALYA SADEARNALP PIPRVMWPPG FGAAETVLEG SDGTRFVFGH 1210 1220 1230 1240 1250 1260 HGGSERPSET QAGRQRRTAD DREHALELDD WEVGCEDAWD SEEGGGDDGD APGSSFGVSI 1270 1280 1290 1300 1310 VSVAPGVLRD RRVGLRPAVK VELLSSSSSS EDEDDVWGGR GGRSPPQSRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of varicella-zoster virus." Davison A.J., Scott J.E. J. Gen. Virol. 67:1759-1816(1986) [PubMed: 3018124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"DNA sequence of the major inverted repeat in the varicella-zoster virus genome." Davison A.J., Scott J.E. J. Gen. Virol. 66:207-220(1985) [PubMed: 2981964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Varicella-zoster virus complements herpes simplex virus type 1 temperature-sensitive mutants." Felser J.M., Straus S.E., Ostrove J.M. J. Virol. 61:225-228(1987) [PubMed: 3023701] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Cell lines containing varicella-zoster virus open reading frame 62 and expressing the 'IE' 175 protein complement ICP4 mutants of herpes simplex virus type 1." Felser J.M., Kinchington P.R., Inchauspe G., Straus S.E., Ostrove J.M. J. Virol. 62:2076-2082(1988) [PubMed: 2835512] [Abstract] Cited for: FUNCTION.
[5]	"Mutation of a single lysine residue severely impairs the DNA recognition and regulatory functions of the VZV gene 62 transactivator protein." Tyler J.K., Allen K.E., Everett R.D. Nucleic Acids Res. 22:270-278(1994) [PubMed: 7907417] [Abstract] Cited for: MUTAGENESIS OF GLN-545; ASN-546 AND LYS-548.
[6]	"Physical interaction between two varicella zoster virus gene regulatory proteins, IE4 and IE62." Spengler M.L., Ruyechan W.T., Hay J. Virology 272:375-381(2000) [PubMed: 10873781] [Abstract] Cited for: INTERACTION WITH IE4.
[7]	"Nuclear accumulation of IE62, the varicella-zoster virus (VZV) major transcriptional regulatory protein, is inhibited by phosphorylation mediated by the VZV open reading frame 66 protein kinase." Kinchington P.R., Fite K., Turse S.E. J. Virol. 74:2265-2277(2000) [PubMed: 10666257] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Virion association of IE62, the varicella-zoster virus (VZV) major transcriptional regulatory protein, requires expression of the VZV open reading frame 66 protein kinase." Kinchington P.R., Fite K., Seman A., Turse S.E. J. Virol. 75:9106-9113(2001) [PubMed: 11533174] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Varicella-zoster virus ORF47 protein serine kinase: characterization of a cloned, biologically active phosphotransferase and two viral substrates, ORF62 and ORF63." Kenyon T.K., Lynch J.M., Hay J., Ruyechan W.T., Grose C. J. Virol. 75:8854-8858(2001) [PubMed: 11507231] [Abstract] Cited for: PHOSPHORYLATION.
[10]	"Physical and functional interaction between the varicella zoster virus IE63 and IE62 proteins." Lynch J.M., Kenyon T.K., Grose C., Hay J., Ruyechan W.T. Virology 302:71-82(2002) [PubMed: 12429517] [Abstract] Cited for: INTERACTION WITH IE63.
[11]	"Transcription factor USF, expressed during the entire phase of Varicella-zoster virus infection, interacts physically with the major viral transactivator IE62 and plays a significant role in virus replication." Rahaus M., Desloges N., Yang M., Ruyechan W.T., Wolff M.H. J. Gen. Virol. 84:2957-2967(2003) [PubMed: 14573800] [Abstract] Cited for: INTERACTION WITH HUMAN USF1 PROTEIN.
[12]	"Interaction between the varicella zoster virus IE62 major transactivator and cellular transcription factor Sp1." Peng H., He H., Hay J., Ruyechan W.T. J. Biol. Chem. 278:38068-38075(2003) [PubMed: 12855699] [Abstract] Cited for: INTERACTION WITH HUMAN SP1 PROTEIN.
[13]	"Phosphorylation of the varicella-zoster virus (VZV) major transcriptional regulatory protein IE62 by the VZV open reading frame 66 protein kinase." Eisfeld A.J., Turse S.E., Jackson S.A., Lerner E.C., Kinchington P.R. J. Virol. 80:1710-1723(2006) [PubMed: 16439528] [Abstract] Cited for: NUCLEAR LOCALIZATION SIGNAL, PHOSPHORYLATION AT SER-686 AND SER-722.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04370 Genomic DNA. Translation: CAA27954.1. X04370 Genomic DNA. Translation: CAA27945.1. X02132 Genomic DNA. Translation: CAA26044.1.
PIR	WZBE62. A27345.
RefSeq	NP_040184.1. NP_040193.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1487695. 1487699.
Family and domain databases
InterPro	IPR005205. Herpes_ICP4_C. IPR005206. Herpes_ICP4_N. [Graphical view]
Pfam	PF03585. Herpes_ICP4_C. 1 hit. PF03584. Herpes_ICP4_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICP4_VZVD

Accession

Primary (citable) accession number: P09310

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 24, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)