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Protein

Large tegument protein deneddylase

Gene

ORF22

Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Large tegument protein that plays multiple roles in the viral cycle. During viral entry, remains associated with the capsid while most of the tegument is detached and participates in the capsid transport toward the host nucleus. Plays a role in the routing of the capsid at the nuclear pore complex and subsequent uncoating. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favorable environment allowing efficient viral genome replication. Participates later in the secondary envelopment of capsids. Indeed, plays a linker role for the association of the outer viral tegument to the capsids.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei19 – 191Important for catalytic activityPROSITE-ProRule annotation
Active sitei32 – 321PROSITE-ProRule annotation
Active sitei168 – 1681PROSITE-ProRule annotation
Active sitei170 – 1701PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Large tegument protein deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
ORF Names:ORF22
OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri10338 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002602 Componenti: Genome

Subcellular locationi

  1. Virion tegument
  2. Host cytoplasm
  3. Host nucleus By similarity

  4. Note: Tightly associated with the capsid.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 27632763Large tegument protein deneddylasePRO_0000116037Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with ORF21. Interacts with ORF10; this interaction is important for outer tegument association to the capsid. Interacts with capsid vertex specific component (CVSC) ORF34. Interacts with the major capsid protein ORF40.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 237226Peptidase C76PROSITE-ProRule annotationAdd
BLAST
Repeati2458 – 246031
Repeati2461 – 246332
Repeati2464 – 246633
Repeati2467 – 246934
Repeati2470 – 247235

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 247247Deubiquitination activityBy similarityAdd
BLAST
Regioni2458 – 2472155 X 3 AA repeats of P-A-QAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi331 – 3377Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi331 – 3355Poly-Arg
Compositional biasi1367 – 13704Poly-Leu
Compositional biasi2356 – 23594Poly-Leu

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09278-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIIPPIAVT VAGVGSRNQF DGALGPASGL SCLRTSLSFL HMTYAHGINA
60 70 80 90 100
TLSSDMIDGC LQEGAAWTTD LSNMGRGVPD MCALVDLPNR ISYIKLGDTT
110 120 130 140 150
STCCVLSRIY GDSHFFTVPD EGFMCTQIPA RAFFDDVWMG REESYTIITV
160 170 180 190 200
DSTGMAIYRQ GNISFIFDPH GHGTIGQAVV VRVNTTDVYS YIASEYTHRP
210 220 230 240 250
DNVESQWAAA LVFFVTANDG PVSEEALSSA VTLIYGSCDT YFTDEQYCEK
260 270 280 290 300
LVTAQHPLLL SPPNSTTIVL NKSSIVPLHQ NVGESVSLEA TLHSTLTNTV
310 320 330 340 350
ALDPRCSYSE VDPWHAVLET TSTGSGVLDC RRRRRPSWTP PSSEENLACI
360 370 380 390 400
DDGLVNNTHS TDNLHKPAKK VLKFKPTVDV PDKTQVAHVL PRLREVANTP
410 420 430 440 450
DVVLNVSNVD TPESSPTFSR NMNVGSSLKD RKPFLFEQSG DVNMVVEKLL
460 470 480 490 500
QHGHEISNGY VQNAVGTLDT VITGHTNVPI WVTRPLVMPD EKDPLELFIN
510 520 530 540 550
LTILRLTGFV VENGTRTHHG ATSVVSDFIG PLGEILTGFP SAAELIRVTS
560 570 580 590 600
LILTNMPGAE YAIKTVLRKK CTIGMLIIAK FGLVAMRVQD TTGALHAELD
610 620 630 640 650
VLEADLGGSS PIDLYSRLST GLISILNSPI ISHPGLFAEL IPTRTGSLSE
660 670 680 690 700
RIRLLCELVS ARETRYMREH TALVSSVKAL ENALRSTRNK IDAIQIPEVP
710 720 730 740 750
QEPPEETDIP PEELIRRVYE IRSEVTMLLT SAVTEYFTRG VLYSTRALIA
760 770 780 790 800
EQSPRRFRVA TASTAPIQRL LDSLPEFDAK LTAIISSLSI HPPPETIQNL
810 820 830 840 850
PVVSLLKELI KEGEDLNTDT ALVSWLSVVG EAQTAGYLSR REFDELSRTI
860 870 880 890 900
KTINTRATQR ASAEAELSCF NTLSAAVDQA VKDYETYNNG EVKYPEITRD
910 920 930 940 950
DLLATIVRAT DDLVRQIKIL SDPMIQSGLQ PSIKRRLETR LKEVQTYANE
960 970 980 990 1000
ARTTQDTIKS RKQAAYNKLG GLLRPVTGFV GLRAAVDLLP ELASELDVQG
1010 1020 1030 1040 1050
ALVNLRTKVL EAPVEIRSQL TGDFWALFNQ YRDILEHPGN ARTSVLGGLG
1060 1070 1080 1090 1100
ACFTAIIEIV PIPTEYRPSL LAFFGDVADV LASDIATVST NPESESAINA
1110 1120 1130 1140 1150
VVATLSKATL VSSTVPALSF VLSLYKKYQA LQQEITNTHK LTELQKQLGD
1160 1170 1180 1190 1200
DFSTLAVSSG HLKFISSSNV DDYEINDAIL SIQTNVHALM DTVKLVEVEL
1210 1220 1230 1240 1250
QKLPPHCIAG TSTLSRVVKD LHKLVTMAHE KKEQAKVLIT DCERAHKQQT
1260 1270 1280 1290 1300
TRVLYERWTR DIIACLEAME TRHIFNGTEL ARLRDMAAAG GFDIHAVYPQ
1310 1320 1330 1340 1350
ARQVVAACET TAVTALDTVF RHNPYTPENT NIPPPLALLR GLTWFDDFSI
1360 1370 1380 1390 1400
TAPVFTVMFP GVSIEGLLLL MRIRAVVLLS ADTSINGIPN YRDMILRTSG
1410 1420 1430 1440 1450
DLLQIPALAG YVDFYTRSYD QFITESVTLS ELRADIRQAA GAKLTEANKA
1460 1470 1480 1490 1500
LEEVTHVRAH ETAKLALKEG VFITLPSEGL LIRAIEYFTT FDHKRFIGTA
1510 1520 1530 1540 1550
YERVLQTMVD RDLKEANAEL AQFRMVCQAT KNRAIQILQN IVDTANATEQ
1560 1570 1580 1590 1600
QEDVDFTNLK TLLKLTPPPK TIALAIDRST SVQDIVTQFA LLLGRLEEET
1610 1620 1630 1640 1650
GTLDIQAVDW MYQARNIIDS HPLSVRIDGT GPLHTYKDRV DKLYALRTKL
1660 1670 1680 1690 1700
DLLRRRIETG EVTWDDAWTT FKRETGDMLA SGDTYATSVD SIKALQASAS
1710 1720 1730 1740 1750
VVDMLCSEPE FFLLPVETKN RLQKKQQERK TALDVVLQKQ RQFEETASRL
1760 1770 1780 1790 1800
RALIERIPTE SDHDVLRMLL RDFDQFTHLP IWIKTQYMTF RNLLMVRLGL
1810 1820 1830 1840 1850
YASYAEIFPP ASPNGVFAPI PAMSGVCLED QSRCIRARVA AFMGEASVVQ
1860 1870 1880 1890 1900
TFREARSSID ALFGKNLTFY LDTDGVPLRY RVCYKSVGVK LGTMLCSQGG
1910 1920 1930 1940 1950
LSLRPALPDE GIVEETTLSA LRVANEVNEL RIEYESAIKS GFSAFSTFVR
1960 1970 1980 1990 2000
HRHAEWGKTN ARRAIAEIYA GLITTTLTRQ YGVHWDKLIY SFEKHHLTSV
2010 2020 2030 2040 2050
MGNGLTKPIQ RRGDVRVLEL TLSDIVTILV ATTPVHLLNF ARLDLIKQHE
2060 2070 2080 2090 2100
YMARTLRPVI EAAFRGRLLV RSLDGDPKGN ARAFFNAAPS KHKLPLALGS
2110 2120 2130 2140 2150
NQDPTGGRIF AFRMADWKLV KMPQKITDPF APWQLSPPPG VKANVDAVTR
2160 2170 2180 2190 2200
IMATDRLATI TVLGRMCLPP ISLVSMWNTL QPEEFAYRTQ DDVDIIVDAR
2210 2220 2230 2240 2250
LDLSSTLNAR FDTAPSNTTL EWNTDRKVIT DAYIQTGATT VFTVTGAAPT
2260 2270 2280 2290 2300
HVSNVTAFDI ATTAILFGAP LVIAMELTSV FSQNSGLTLG LKLFDSRHMA
2310 2320 2330 2340 2350
TDSGISSAVS PDIVSWGLRL LHMDPHPIEN ACLIVQLEKL SALIANKPLT
2360 2370 2380 2390 2400
NNPPCLLLLD EHMNPSYVLW ERKDSIPAPD YVVFWGPESL IDLPYIDSDE
2410 2420 2430 2440 2450
DSFPSCPDDP FYSQIIAGYA PQGPPNLDTT DFYPTEPLFK SPVQVVRSSK
2460 2470 2480 2490 2500
CKKMPVRPAQ PAQPAQPAQP AQTVQPAQPI EPGTQIVVQN FKKPQSVKTT
2510 2520 2530 2540 2550
LSQKDIPLYV ETESETAVLI PKQLTTSIKT TVCKSITPPN NQLSDWKNNP
2560 2570 2580 2590 2600
QQNQTLNQAF SKPILEITSI PTDDSISYRT WIEKSNQTQK RHQNDPRMYN
2610 2620 2630 2640 2650
SKTVFHPVNN QLPSWVDTAA DAPQTDLLTN YKTRQPSPNF PRDVHTWGVS
2660 2670 2680 2690 2700
SNPFNSPNRD LYQSDFSEPS DGYSSESENS IVLSLDEHRS CRVPRHVRVV
2710 2720 2730 2740 2750
NADVVTGRRY VRGTALGALA LLSQACRRMI DNVRYTRKLL MDHTEDIFQG
2760
LGYVKLLLDG TYI
Length:2,763
Mass (Da):306,343
Last modified:July 1, 1989 - v1
Checksum:i0995F7745B9542F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27905.1.
PIRiD27343. WZBE22.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27905.1.
PIRiD27343. WZBE22.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete DNA sequence of varicella-zoster virus."
    Davison A.J., Scott J.E.
    J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiLTP_VZVD
AccessioniPrimary (citable) accession number: P09278
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 29, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.