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P09278

- DEN_VZVD

UniProt

P09278 - DEN_VZVD

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Protein

Deneddylase

Gene
ORF22
Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei19 – 191Important for catalytic activity By similarity
Active sitei32 – 321 By similarity
Active sitei168 – 1681 By similarity
Active sitei170 – 1701 By similarity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: InterPro
  2. omega peptidase activity Source: InterPro

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
ORF Names:ORF22
OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri10338 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002602: Genome

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid By similarity.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 27632763DeneddylasePRO_0000116037Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Structurei

3D structure databases

ProteinModelPortaliP09278.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 237226Peptidase C76Add
BLAST
Repeati2458 – 246031
Repeati2461 – 246332
Repeati2464 – 246633
Repeati2467 – 246934
Repeati2470 – 247235

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 247247Deubiquitination activity By similarityAdd
BLAST
Regioni2458 – 2472155 X 3 AA repeats of P-A-QAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi331 – 3377Nuclear localization signal By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi331 – 3355Poly-Arg
Compositional biasi1367 – 13704Poly-Leu
Compositional biasi2356 – 23594Poly-Leu

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09278-1 [UniParc]FASTAAdd to Basket

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MDIIPPIAVT VAGVGSRNQF DGALGPASGL SCLRTSLSFL HMTYAHGINA     50
TLSSDMIDGC LQEGAAWTTD LSNMGRGVPD MCALVDLPNR ISYIKLGDTT 100
STCCVLSRIY GDSHFFTVPD EGFMCTQIPA RAFFDDVWMG REESYTIITV 150
DSTGMAIYRQ GNISFIFDPH GHGTIGQAVV VRVNTTDVYS YIASEYTHRP 200
DNVESQWAAA LVFFVTANDG PVSEEALSSA VTLIYGSCDT YFTDEQYCEK 250
LVTAQHPLLL SPPNSTTIVL NKSSIVPLHQ NVGESVSLEA TLHSTLTNTV 300
ALDPRCSYSE VDPWHAVLET TSTGSGVLDC RRRRRPSWTP PSSEENLACI 350
DDGLVNNTHS TDNLHKPAKK VLKFKPTVDV PDKTQVAHVL PRLREVANTP 400
DVVLNVSNVD TPESSPTFSR NMNVGSSLKD RKPFLFEQSG DVNMVVEKLL 450
QHGHEISNGY VQNAVGTLDT VITGHTNVPI WVTRPLVMPD EKDPLELFIN 500
LTILRLTGFV VENGTRTHHG ATSVVSDFIG PLGEILTGFP SAAELIRVTS 550
LILTNMPGAE YAIKTVLRKK CTIGMLIIAK FGLVAMRVQD TTGALHAELD 600
VLEADLGGSS PIDLYSRLST GLISILNSPI ISHPGLFAEL IPTRTGSLSE 650
RIRLLCELVS ARETRYMREH TALVSSVKAL ENALRSTRNK IDAIQIPEVP 700
QEPPEETDIP PEELIRRVYE IRSEVTMLLT SAVTEYFTRG VLYSTRALIA 750
EQSPRRFRVA TASTAPIQRL LDSLPEFDAK LTAIISSLSI HPPPETIQNL 800
PVVSLLKELI KEGEDLNTDT ALVSWLSVVG EAQTAGYLSR REFDELSRTI 850
KTINTRATQR ASAEAELSCF NTLSAAVDQA VKDYETYNNG EVKYPEITRD 900
DLLATIVRAT DDLVRQIKIL SDPMIQSGLQ PSIKRRLETR LKEVQTYANE 950
ARTTQDTIKS RKQAAYNKLG GLLRPVTGFV GLRAAVDLLP ELASELDVQG 1000
ALVNLRTKVL EAPVEIRSQL TGDFWALFNQ YRDILEHPGN ARTSVLGGLG 1050
ACFTAIIEIV PIPTEYRPSL LAFFGDVADV LASDIATVST NPESESAINA 1100
VVATLSKATL VSSTVPALSF VLSLYKKYQA LQQEITNTHK LTELQKQLGD 1150
DFSTLAVSSG HLKFISSSNV DDYEINDAIL SIQTNVHALM DTVKLVEVEL 1200
QKLPPHCIAG TSTLSRVVKD LHKLVTMAHE KKEQAKVLIT DCERAHKQQT 1250
TRVLYERWTR DIIACLEAME TRHIFNGTEL ARLRDMAAAG GFDIHAVYPQ 1300
ARQVVAACET TAVTALDTVF RHNPYTPENT NIPPPLALLR GLTWFDDFSI 1350
TAPVFTVMFP GVSIEGLLLL MRIRAVVLLS ADTSINGIPN YRDMILRTSG 1400
DLLQIPALAG YVDFYTRSYD QFITESVTLS ELRADIRQAA GAKLTEANKA 1450
LEEVTHVRAH ETAKLALKEG VFITLPSEGL LIRAIEYFTT FDHKRFIGTA 1500
YERVLQTMVD RDLKEANAEL AQFRMVCQAT KNRAIQILQN IVDTANATEQ 1550
QEDVDFTNLK TLLKLTPPPK TIALAIDRST SVQDIVTQFA LLLGRLEEET 1600
GTLDIQAVDW MYQARNIIDS HPLSVRIDGT GPLHTYKDRV DKLYALRTKL 1650
DLLRRRIETG EVTWDDAWTT FKRETGDMLA SGDTYATSVD SIKALQASAS 1700
VVDMLCSEPE FFLLPVETKN RLQKKQQERK TALDVVLQKQ RQFEETASRL 1750
RALIERIPTE SDHDVLRMLL RDFDQFTHLP IWIKTQYMTF RNLLMVRLGL 1800
YASYAEIFPP ASPNGVFAPI PAMSGVCLED QSRCIRARVA AFMGEASVVQ 1850
TFREARSSID ALFGKNLTFY LDTDGVPLRY RVCYKSVGVK LGTMLCSQGG 1900
LSLRPALPDE GIVEETTLSA LRVANEVNEL RIEYESAIKS GFSAFSTFVR 1950
HRHAEWGKTN ARRAIAEIYA GLITTTLTRQ YGVHWDKLIY SFEKHHLTSV 2000
MGNGLTKPIQ RRGDVRVLEL TLSDIVTILV ATTPVHLLNF ARLDLIKQHE 2050
YMARTLRPVI EAAFRGRLLV RSLDGDPKGN ARAFFNAAPS KHKLPLALGS 2100
NQDPTGGRIF AFRMADWKLV KMPQKITDPF APWQLSPPPG VKANVDAVTR 2150
IMATDRLATI TVLGRMCLPP ISLVSMWNTL QPEEFAYRTQ DDVDIIVDAR 2200
LDLSSTLNAR FDTAPSNTTL EWNTDRKVIT DAYIQTGATT VFTVTGAAPT 2250
HVSNVTAFDI ATTAILFGAP LVIAMELTSV FSQNSGLTLG LKLFDSRHMA 2300
TDSGISSAVS PDIVSWGLRL LHMDPHPIEN ACLIVQLEKL SALIANKPLT 2350
NNPPCLLLLD EHMNPSYVLW ERKDSIPAPD YVVFWGPESL IDLPYIDSDE 2400
DSFPSCPDDP FYSQIIAGYA PQGPPNLDTT DFYPTEPLFK SPVQVVRSSK 2450
CKKMPVRPAQ PAQPAQPAQP AQTVQPAQPI EPGTQIVVQN FKKPQSVKTT 2500
LSQKDIPLYV ETESETAVLI PKQLTTSIKT TVCKSITPPN NQLSDWKNNP 2550
QQNQTLNQAF SKPILEITSI PTDDSISYRT WIEKSNQTQK RHQNDPRMYN 2600
SKTVFHPVNN QLPSWVDTAA DAPQTDLLTN YKTRQPSPNF PRDVHTWGVS 2650
SNPFNSPNRD LYQSDFSEPS DGYSSESENS IVLSLDEHRS CRVPRHVRVV 2700
NADVVTGRRY VRGTALGALA LLSQACRRMI DNVRYTRKLL MDHTEDIFQG 2750
LGYVKLLLDG TYI 2763
Length:2,763
Mass (Da):306,343
Last modified:July 1, 1989 - v1
Checksum:i0995F7745B9542F5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04370 Genomic DNA. Translation: CAA27905.1.
PIRiD27343. WZBE22.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04370 Genomic DNA. Translation: CAA27905.1 .
PIRi D27343. WZBE22.

3D structure databases

ProteinModelPortali P09278.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete DNA sequence of varicella-zoster virus."
    Davison A.J., Scott J.E.
    J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_VZVD
AccessioniPrimary (citable) accession number: P09278
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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