P09278 (DEN_VZVD) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 69.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Deneddylase ORF22 EC=3.4.19.12 EC=3.4.22.- Alternative name(s): Ubiquitin thioesterase | ||
| Gene names |
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| Organism | Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3) [Reference proteome] | ||
| Taxonomic identifier | 10338 [NCBI] | ||
| Taxonomic lineage | Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus ›  | ||
| Virus host | Homo sapiens (Human) [TaxID: 9606] |
Protein attributes
| Sequence length | 2763 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity. |
| Subcellular location | Virion tegument. Host cytoplasm. Host nucleus By similarity. Note: Tightly associated with the capsid By similarity. |
| Sequence similarities | Belongs to the herpesviridae large tegument protein family. Contains 1 peptidase C76 domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 2763 | 2763 | Deneddylase ORF22 | PRO_0000116037 | |||||
Regions | |||||||||
| Domain | 12 – 237 | 226 | Peptidase C76 | ||||||
| Repeat | 2458 – 2460 | 3 | 1 | ||||||
| Repeat | 2461 – 2463 | 3 | 2 | ||||||
| Repeat | 2464 – 2466 | 3 | 3 | ||||||
| Repeat | 2467 – 2469 | 3 | 4 | ||||||
| Repeat | 2470 – 2472 | 3 | 5 | ||||||
| Region | 1 – 247 | 247 | Deubiquitination activity By similarity | ||||||
| Region | 2458 – 2472 | 15 | 5 X 3 AA repeats of P-A-Q | ||||||
| Motif | 331 – 337 | 7 | Nuclear localization signal By similarity | ||||||
| Compositional bias | 331 – 335 | 5 | Poly-Arg | ||||||
| Compositional bias | 1367 – 1370 | 4 | Poly-Leu | ||||||
| Compositional bias | 2356 – 2359 | 4 | Poly-Leu | ||||||
Sites | |||||||||
| Active site | 32 | 1 | By similarity | ||||||
| Active site | 168 | 1 | By similarity | ||||||
| Active site | 170 | 1 | By similarity | ||||||
| Site | 19 | 1 | Important for catalytic activity By similarity | ||||||
Sequences
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References
| [1] | "The complete DNA sequence of varicella-zoster virus." Davison A.J., Scott J.E. J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X04370 Genomic DNA. Translation: CAA27905.1. |
| PIR | WZBE22. D27343. |
3D structure databases | |
| ProteinModelPortal | P09278. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Phylogenomic databases | |
| ProtClustDB | CLSP2509604. |
Family and domain databases | |
| InterPro | IPR005210. Herpes_UL36. IPR006928. Pept_C76_UL36-USP. [Graphical view] |
| Pfam | PF04843. Herpes_teg_N. 1 hit. PF03586. Herpes_UL36. 1 hit. [Graphical view] |
| PROSITE | PS51521. HTUSP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DEN_VZVD | ||||||||
| Accession | Primary (citable) accession number: P09278 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Viral Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |