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P09278 (DEN_VZVD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 26, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Ubiquitin thioesterase
Gene names
ORF Names:ORF22
OrganismVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3) [Reference proteome]
Taxonomic identifier10338 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length2763 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity. Note: Tightly associated with the capsid By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 27632763Deneddylase
PRO_0000116037

Regions

Domain12 – 237226Peptidase C76
Repeat2458 – 246031
Repeat2461 – 246332
Repeat2464 – 246633
Repeat2467 – 246934
Repeat2470 – 247235
Region1 – 247247Deubiquitination activity By similarity
Region2458 – 2472155 X 3 AA repeats of P-A-Q
Motif331 – 3377Nuclear localization signal By similarity
Compositional bias331 – 3355Poly-Arg
Compositional bias1367 – 13704Poly-Leu
Compositional bias2356 – 23594Poly-Leu

Sites

Active site321 By similarity
Active site1681 By similarity
Active site1701 By similarity
Site191Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P09278 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 0995F7745B9542F5

FASTA2,763306,343
        10         20         30         40         50         60 
MDIIPPIAVT VAGVGSRNQF DGALGPASGL SCLRTSLSFL HMTYAHGINA TLSSDMIDGC 

        70         80         90        100        110        120 
LQEGAAWTTD LSNMGRGVPD MCALVDLPNR ISYIKLGDTT STCCVLSRIY GDSHFFTVPD 

       130        140        150        160        170        180 
EGFMCTQIPA RAFFDDVWMG REESYTIITV DSTGMAIYRQ GNISFIFDPH GHGTIGQAVV 

       190        200        210        220        230        240 
VRVNTTDVYS YIASEYTHRP DNVESQWAAA LVFFVTANDG PVSEEALSSA VTLIYGSCDT 

       250        260        270        280        290        300 
YFTDEQYCEK LVTAQHPLLL SPPNSTTIVL NKSSIVPLHQ NVGESVSLEA TLHSTLTNTV 

       310        320        330        340        350        360 
ALDPRCSYSE VDPWHAVLET TSTGSGVLDC RRRRRPSWTP PSSEENLACI DDGLVNNTHS 

       370        380        390        400        410        420 
TDNLHKPAKK VLKFKPTVDV PDKTQVAHVL PRLREVANTP DVVLNVSNVD TPESSPTFSR 

       430        440        450        460        470        480 
NMNVGSSLKD RKPFLFEQSG DVNMVVEKLL QHGHEISNGY VQNAVGTLDT VITGHTNVPI 

       490        500        510        520        530        540 
WVTRPLVMPD EKDPLELFIN LTILRLTGFV VENGTRTHHG ATSVVSDFIG PLGEILTGFP 

       550        560        570        580        590        600 
SAAELIRVTS LILTNMPGAE YAIKTVLRKK CTIGMLIIAK FGLVAMRVQD TTGALHAELD 

       610        620        630        640        650        660 
VLEADLGGSS PIDLYSRLST GLISILNSPI ISHPGLFAEL IPTRTGSLSE RIRLLCELVS 

       670        680        690        700        710        720 
ARETRYMREH TALVSSVKAL ENALRSTRNK IDAIQIPEVP QEPPEETDIP PEELIRRVYE 

       730        740        750        760        770        780 
IRSEVTMLLT SAVTEYFTRG VLYSTRALIA EQSPRRFRVA TASTAPIQRL LDSLPEFDAK 

       790        800        810        820        830        840 
LTAIISSLSI HPPPETIQNL PVVSLLKELI KEGEDLNTDT ALVSWLSVVG EAQTAGYLSR 

       850        860        870        880        890        900 
REFDELSRTI KTINTRATQR ASAEAELSCF NTLSAAVDQA VKDYETYNNG EVKYPEITRD 

       910        920        930        940        950        960 
DLLATIVRAT DDLVRQIKIL SDPMIQSGLQ PSIKRRLETR LKEVQTYANE ARTTQDTIKS 

       970        980        990       1000       1010       1020 
RKQAAYNKLG GLLRPVTGFV GLRAAVDLLP ELASELDVQG ALVNLRTKVL EAPVEIRSQL 

      1030       1040       1050       1060       1070       1080 
TGDFWALFNQ YRDILEHPGN ARTSVLGGLG ACFTAIIEIV PIPTEYRPSL LAFFGDVADV 

      1090       1100       1110       1120       1130       1140 
LASDIATVST NPESESAINA VVATLSKATL VSSTVPALSF VLSLYKKYQA LQQEITNTHK 

      1150       1160       1170       1180       1190       1200 
LTELQKQLGD DFSTLAVSSG HLKFISSSNV DDYEINDAIL SIQTNVHALM DTVKLVEVEL 

      1210       1220       1230       1240       1250       1260 
QKLPPHCIAG TSTLSRVVKD LHKLVTMAHE KKEQAKVLIT DCERAHKQQT TRVLYERWTR 

      1270       1280       1290       1300       1310       1320 
DIIACLEAME TRHIFNGTEL ARLRDMAAAG GFDIHAVYPQ ARQVVAACET TAVTALDTVF 

      1330       1340       1350       1360       1370       1380 
RHNPYTPENT NIPPPLALLR GLTWFDDFSI TAPVFTVMFP GVSIEGLLLL MRIRAVVLLS 

      1390       1400       1410       1420       1430       1440 
ADTSINGIPN YRDMILRTSG DLLQIPALAG YVDFYTRSYD QFITESVTLS ELRADIRQAA 

      1450       1460       1470       1480       1490       1500 
GAKLTEANKA LEEVTHVRAH ETAKLALKEG VFITLPSEGL LIRAIEYFTT FDHKRFIGTA 

      1510       1520       1530       1540       1550       1560 
YERVLQTMVD RDLKEANAEL AQFRMVCQAT KNRAIQILQN IVDTANATEQ QEDVDFTNLK 

      1570       1580       1590       1600       1610       1620 
TLLKLTPPPK TIALAIDRST SVQDIVTQFA LLLGRLEEET GTLDIQAVDW MYQARNIIDS 

      1630       1640       1650       1660       1670       1680 
HPLSVRIDGT GPLHTYKDRV DKLYALRTKL DLLRRRIETG EVTWDDAWTT FKRETGDMLA 

      1690       1700       1710       1720       1730       1740 
SGDTYATSVD SIKALQASAS VVDMLCSEPE FFLLPVETKN RLQKKQQERK TALDVVLQKQ 

      1750       1760       1770       1780       1790       1800 
RQFEETASRL RALIERIPTE SDHDVLRMLL RDFDQFTHLP IWIKTQYMTF RNLLMVRLGL 

      1810       1820       1830       1840       1850       1860 
YASYAEIFPP ASPNGVFAPI PAMSGVCLED QSRCIRARVA AFMGEASVVQ TFREARSSID 

      1870       1880       1890       1900       1910       1920 
ALFGKNLTFY LDTDGVPLRY RVCYKSVGVK LGTMLCSQGG LSLRPALPDE GIVEETTLSA 

      1930       1940       1950       1960       1970       1980 
LRVANEVNEL RIEYESAIKS GFSAFSTFVR HRHAEWGKTN ARRAIAEIYA GLITTTLTRQ 

      1990       2000       2010       2020       2030       2040 
YGVHWDKLIY SFEKHHLTSV MGNGLTKPIQ RRGDVRVLEL TLSDIVTILV ATTPVHLLNF 

      2050       2060       2070       2080       2090       2100 
ARLDLIKQHE YMARTLRPVI EAAFRGRLLV RSLDGDPKGN ARAFFNAAPS KHKLPLALGS 

      2110       2120       2130       2140       2150       2160 
NQDPTGGRIF AFRMADWKLV KMPQKITDPF APWQLSPPPG VKANVDAVTR IMATDRLATI 

      2170       2180       2190       2200       2210       2220 
TVLGRMCLPP ISLVSMWNTL QPEEFAYRTQ DDVDIIVDAR LDLSSTLNAR FDTAPSNTTL 

      2230       2240       2250       2260       2270       2280 
EWNTDRKVIT DAYIQTGATT VFTVTGAAPT HVSNVTAFDI ATTAILFGAP LVIAMELTSV 

      2290       2300       2310       2320       2330       2340 
FSQNSGLTLG LKLFDSRHMA TDSGISSAVS PDIVSWGLRL LHMDPHPIEN ACLIVQLEKL 

      2350       2360       2370       2380       2390       2400 
SALIANKPLT NNPPCLLLLD EHMNPSYVLW ERKDSIPAPD YVVFWGPESL IDLPYIDSDE 

      2410       2420       2430       2440       2450       2460 
DSFPSCPDDP FYSQIIAGYA PQGPPNLDTT DFYPTEPLFK SPVQVVRSSK CKKMPVRPAQ 

      2470       2480       2490       2500       2510       2520 
PAQPAQPAQP AQTVQPAQPI EPGTQIVVQN FKKPQSVKTT LSQKDIPLYV ETESETAVLI 

      2530       2540       2550       2560       2570       2580 
PKQLTTSIKT TVCKSITPPN NQLSDWKNNP QQNQTLNQAF SKPILEITSI PTDDSISYRT 

      2590       2600       2610       2620       2630       2640 
WIEKSNQTQK RHQNDPRMYN SKTVFHPVNN QLPSWVDTAA DAPQTDLLTN YKTRQPSPNF 

      2650       2660       2670       2680       2690       2700 
PRDVHTWGVS SNPFNSPNRD LYQSDFSEPS DGYSSESENS IVLSLDEHRS CRVPRHVRVV 

      2710       2720       2730       2740       2750       2760 
NADVVTGRRY VRGTALGALA LLSQACRRMI DNVRYTRKLL MDHTEDIFQG LGYVKLLLDG 


TYI 

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References

[1]"The complete DNA sequence of varicella-zoster virus."
Davison A.J., Scott J.E.
J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04370 Genomic DNA. Translation: CAA27905.1.
PIRWZBE22. D27343.

3D structure databases

ProteinModelPortalP09278.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

ProtClustDBCLSP2509604.

Family and domain databases

InterProIPR005210. Herpes_UL36.
IPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
PF03586. Herpes_UL36. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_VZVD
AccessionPrimary (citable) accession number: P09278
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 26, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries