P09255 (ICP22_VZVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional regulator IE63

Alternative name(s):
Immediate-early protein 63
Short name=IE63

Gene names

ORF Names:	ORF63
AND
ORF Names:	ORF70

Organism

Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3) [Reference proteome]

Taxonomic identifier

10338 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus › Human herpesvirus 3

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Immediate early (EI) protein that functions as a transcriptional regulator of cellular and viral mRNAs mainly by interacting with several general transcription factors thereby disorganizing the preinitiation complex at certain promoters. May additionally help to regulate levels of histones in virus-infected cells by interacting with host ASF1. By inhibiting host transcriptional program, IE63 plays a major role in the ability of VZV to overcome the innate immune response to the virus. Ref.3 Ref.7
Subunit structure	Interacts with IE62; this interaction modulates the function of IE62. Interacts with several components of host pre-initiation complex including GTF2E1, GTF2H2 and POLR2A; these interactions lead to repression of gene transcription. Interacts with host ASF1A; altering its ability to bind histones. Ref.6 Ref.7 Ref.8
Subcellular location	Host cytoplasm. Host nucleus. Virion tegument. Note: During the first stage of infection, IE63 is mostly expressed in the nucleus and also slightly in the cytoplasm, and during latency, IE63 localizes in the cytoplasm quite exclusively. Ref.4
Post-translational modification	Phosphorylated in vitro by host and by protein kinase ORF47. Ref.5
Sequence similarities	Belongs to the herpesviridae IE68 family.

Ontologies

Keywords
Biological process	Host gene expression shutoff by virus Host transcription shutoff by virus Host-virus interaction Inhibition of host RNA polymerase II by virus Inhibition of host transcription initiation by virus Transcription Transcription regulation
Cellular component	Host cytoplasm Host nucleus Virion Virion tegument
Developmental stage	Early protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host DNA-dependent transcription, initiation Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host RNA polymerase II activity Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral tegument Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Transcriptional regulator IE63		PRO_0000115843
Regions
	Region	1 – 142	142	IE62-binding

Sequences

	Sequence		Length	Mass (Da)
	P09255 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 2CFA03C199F9A385	FASTA	278	30,495
	10 20 30 40 50 60 MFCTSPATRG DSSESKPGAS VDVNGKMEYG SAPGPLNGRD TSRGPGAFCT PGWEIHPARL 70 80 90 100 110 120 VEDINRVFLC IAQSSGRVTR DSRRLRRICL DFYLMGRTRQ RPTLACWEEL LQLQPTQTQC 130 140 150 160 170 180 LRATLMEVSH RPPRGEDGFI EAPNVPLHRS ALECDVSDDG GEDDSDDDGS TPSDVIEFRD 190 200 210 220 230 240 SDAESSDGED FIVEEESEES TDSCEPDGVP GDCYRDGDGC NTPSPKRPQR AIERYAGAET 250 260 270 AEYTAAKALT ALGEGGVDWK RRRHEAPRRH DIPPPHGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of varicella-zoster virus." Davison A.J., Scott J.E. J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"DNA sequence of the major inverted repeat in the varicella-zoster virus genome." Davison A.J., Scott J.E. J. Gen. Virol. 66:207-220(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization of regulatory functions of the varicella-zoster virus gene 63-encoded protein." Jackers P., Defechereux P., Baudoux L., Lambert C., Massaer M., Merville-Louis M.P., Rentier B., Piette J. J. Virol. 66:3899-3903(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Aberrant intracellular localization of Varicella-Zoster virus regulatory proteins during latency." Lungu O., Panagiotidis C.A., Annunziato P.W., Gershon A.A., Silverstein S.J. Proc. Natl. Acad. Sci. U.S.A. 95:7080-7085(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Varicella-zoster virus ORF47 protein serine kinase: characterization of a cloned, biologically active phosphotransferase and two viral substrates, ORF62 and ORF63." Kenyon T.K., Lynch J.M., Hay J., Ruyechan W.T., Grose C. J. Virol. 75:8854-8858(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY ORF47 PROTEIN SERINE KINASE.
[6]	"Physical and functional interaction between the varicella zoster virus IE63 and IE62 proteins." Lynch J.M., Kenyon T.K., Grose C., Hay J., Ruyechan W.T. Virology 302:71-82(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IE62.
[7]	"Varicella-zoster virus IE63 protein represses the basal transcription machinery by disorganizing the pre-initiation complex." Di Valentin E., Bontems S., Habran L., Jolois O., Markine-Goriaynoff N., Vanderplasschen A., Sadzot-Delvaux C., Piette J. Biol. Chem. 386:255-267(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HOST GTF2E1; GTF2H2 AND POLR2A.
[8]	"Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3." Ambagala A.P., Bosma T., Ali M.A., Poustovoitov M., Chen J.J., Gershon M.D., Adams P.D., Cohen J.I. J. Virol. 83:200-209(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HOST ASF1A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04370 Genomic DNA. Translation: CAA27953.1. X04370 Genomic DNA. Translation: CAA27946.1. X02132 Genomic DNA. Translation: CAA26045.1.
PIR	EDBE63. B27345.
RefSeq	NP_040185.1. NC_001348.1. NP_040192.1. NC_001348.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1487700. 1487711.
Phylogenomic databases
ProtClustDB	CLSP2509640.
Family and domain databases
InterPro	IPR003403. IE68. [Graphical view]
Pfam	PF02479. Herpes_IE68. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICP22_VZVD

Accession

Primary (citable) accession number: P09255

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program