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Protein

DNA polymerase catalytic subunit

Gene

ORF28

Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Endonucleolytic cleavage to 5'-phosphomonoester.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase catalytic subunit (EC:2.7.7.7, EC:3.1.26.4)
Gene namesi
ORF Names:ORF28
OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri10338 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000002602 Componenti: Genome

Subcellular locationi

  • Host nucleus By similarity

  • Note: the protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4859.
DrugBankiDB00787. Aciclovir.
DB00426. Famciclovir.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11941194DNA polymerase catalytic subunitPRO_0000046525Add
BLAST

Interactioni

Subunit structurei

Forms a complex with the ssDNA-binding protein, the DNA polymerase processivity factor, and the alkaline exonuclease. Interacts with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor; this interaction may coordinate leading and lagging strand DNA synthesis at the replication fork (By similarity).By similarity

Chemistry

BindingDBiP09252.

Structurei

3D structure databases

ProteinModelPortaliP09252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIRTGFCNP FLTQASGIKY NPRTGRGSNR EFLHSYKTTM SSFQFLAPKC
60 70 80 90 100
LDEDVPMEER KGVHVGTLSR PPKVYCNGKE VPILDFRCSS PWPRRVNIWG
110 120 130 140 150
EIDFRGDKFD PRFNTFHVYD IVETTEAASN GDVSRFATAT RPLGTVITLL
160 170 180 190 200
GMSRCGKRVA VHVYGICQYF YINKAEVDTA CGIRSGSELS VLLAECLRSS
210 220 230 240 250
MITQNDATLN GDKNAFHGTS FKSASPESFR VEVIERTDVY YYDTQPCAFY
260 270 280 290 300
RVYSPSSKFT NYLCDNFHPE LKKYEGRVDA TTRFLMDNPG FVSFGWYQLK
310 320 330 340 350
PGVDGERVRV RPASRQLTLS DVEIDCMSDN LQAIPNDDSW PDYKLLCFDI
360 370 380 390 400
ECKSGGSNEL AFPDATHLED LVIQISCLLY SIPRQSLEHI LLFSLGSCDL
410 420 430 440 450
PQRYVQEMKD AGLPEPTVLE FDSEFELLIA FMTLVKQYAP EFATGYNIVN
460 470 480 490 500
FDWAFIMEKL NSIYSLKLDG YGSINRGGLF KIWDVGKSGF QRRSKVKING
510 520 530 540 550
LISLDMYAIA TEKLKLSSYK LDSVAREALN ESKRDLPYKD IPGYYASGPN
560 570 580 590 600
TRGIIGEYCI QDSALVGKLF FKYLPHLELS AVARLARITL TKAIYDGQQV
610 620 630 640 650
RIYTCLLGLA SSRGFILPDG GYPATFEYKD VIPDVGDVEE EMDEDESVSP
660 670 680 690 700
TGTSSGRNVG YKGARVFDPD TGFYIDPVVV LDFASLYPSI IQAHNLCFTT
710 720 730 740 750
LTLNFETVKR LNPSDYATFT VGGKRLFFVR SNVRESLLGV LLKDWLAMRK
760 770 780 790 800
AIRARIPGSS SDEAVLLDKQ QAAIKVVCNS VYGFTGVAQG FLPCLYVAAT
810 820 830 840 850
VTTIGRQMLL STRDYIHNNW AAFERFITAF PDIESSVLSQ KAYEVKVIYG
860 870 880 890 900
DTDSVFIRFK GVSVEGIAKI GEKMAHIIST ALFCPPIKLE CEKTFIKLLL
910 920 930 940 950
ITKKKYIGVI YGGKVLMKGV DLVRKNNCQF INDYARKLVE LLLYDDTVSR
960 970 980 990 1000
AAAEASCVSI AEWNRRAMPS GMAGFGRIIA DAHRQITSPK LDINKFVMTA
1010 1020 1030 1040 1050
ELSRPPSAYI NRRLAHLTVY YKLVMRQGQI PNVRERIPYV IVAPTDEVEA
1060 1070 1080 1090 1100
DAKSVALLRG DPLQNTAGKR CGEAKRKLII SDLAEDPIHV TSHGLSLNID
1110 1120 1130 1140 1150
YYFSHLIGTA SVTFKALFGN DTKLTERLLK RFIPETRVVN VKMLNRLQAA
1160 1170 1180 1190
GFVCIHAPCW DNKMNTEAEI TEEEQSHQIM RRVFCIPKAI LHQS
Length:1,194
Mass (Da):134,048
Last modified:July 1, 1989 - v1
Checksum:iC0D5F0EA8D0D81E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27911.1.
PIRiB27214. DJBE28.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27911.1.
PIRiB27214. DJBE28.

3D structure databases

ProteinModelPortaliP09252.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP09252.
ChEMBLiCHEMBL4859.
DrugBankiDB00787. Aciclovir.
DB00426. Famciclovir.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPOL_VZVD
AccessioniPrimary (citable) accession number: P09252
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 27, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.