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P09252 (DPOL_VZVD) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase catalytic subunit
EC=2.7.7.7
EC=3.1.26.4
Gene names
ORF Names:ORF28
OrganismVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Taxonomic identifier10338 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length1194 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Endonucleolytic cleavage to 5'-phosphomonoester.

Subunit structure

Forms a complex with the ssDNA-binding protein, the DNA polymerase processivity factor, and the alkaline exonuclease. Interacts with the helicase-primase complex composed of the primase, the helicase and the primase-associated factor; this interaction may coordinate leading and lagging strand DNA synthesis at the replication fork By similarity.

Subcellular location

Host nucleus By similarity. Note: the protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs By similarity.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11941194DNA polymerase catalytic subunit
PRO_0000046525

Sequences

Sequence LengthMass (Da)Tools
P09252 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: C0D5F0EA8D0D81E0

FASTA1,194134,048
        10         20         30         40         50         60 
MAIRTGFCNP FLTQASGIKY NPRTGRGSNR EFLHSYKTTM SSFQFLAPKC LDEDVPMEER 

        70         80         90        100        110        120 
KGVHVGTLSR PPKVYCNGKE VPILDFRCSS PWPRRVNIWG EIDFRGDKFD PRFNTFHVYD 

       130        140        150        160        170        180 
IVETTEAASN GDVSRFATAT RPLGTVITLL GMSRCGKRVA VHVYGICQYF YINKAEVDTA 

       190        200        210        220        230        240 
CGIRSGSELS VLLAECLRSS MITQNDATLN GDKNAFHGTS FKSASPESFR VEVIERTDVY 

       250        260        270        280        290        300 
YYDTQPCAFY RVYSPSSKFT NYLCDNFHPE LKKYEGRVDA TTRFLMDNPG FVSFGWYQLK 

       310        320        330        340        350        360 
PGVDGERVRV RPASRQLTLS DVEIDCMSDN LQAIPNDDSW PDYKLLCFDI ECKSGGSNEL 

       370        380        390        400        410        420 
AFPDATHLED LVIQISCLLY SIPRQSLEHI LLFSLGSCDL PQRYVQEMKD AGLPEPTVLE 

       430        440        450        460        470        480 
FDSEFELLIA FMTLVKQYAP EFATGYNIVN FDWAFIMEKL NSIYSLKLDG YGSINRGGLF 

       490        500        510        520        530        540 
KIWDVGKSGF QRRSKVKING LISLDMYAIA TEKLKLSSYK LDSVAREALN ESKRDLPYKD 

       550        560        570        580        590        600 
IPGYYASGPN TRGIIGEYCI QDSALVGKLF FKYLPHLELS AVARLARITL TKAIYDGQQV 

       610        620        630        640        650        660 
RIYTCLLGLA SSRGFILPDG GYPATFEYKD VIPDVGDVEE EMDEDESVSP TGTSSGRNVG 

       670        680        690        700        710        720 
YKGARVFDPD TGFYIDPVVV LDFASLYPSI IQAHNLCFTT LTLNFETVKR LNPSDYATFT 

       730        740        750        760        770        780 
VGGKRLFFVR SNVRESLLGV LLKDWLAMRK AIRARIPGSS SDEAVLLDKQ QAAIKVVCNS 

       790        800        810        820        830        840 
VYGFTGVAQG FLPCLYVAAT VTTIGRQMLL STRDYIHNNW AAFERFITAF PDIESSVLSQ 

       850        860        870        880        890        900 
KAYEVKVIYG DTDSVFIRFK GVSVEGIAKI GEKMAHIIST ALFCPPIKLE CEKTFIKLLL 

       910        920        930        940        950        960 
ITKKKYIGVI YGGKVLMKGV DLVRKNNCQF INDYARKLVE LLLYDDTVSR AAAEASCVSI 

       970        980        990       1000       1010       1020 
AEWNRRAMPS GMAGFGRIIA DAHRQITSPK LDINKFVMTA ELSRPPSAYI NRRLAHLTVY 

      1030       1040       1050       1060       1070       1080 
YKLVMRQGQI PNVRERIPYV IVAPTDEVEA DAKSVALLRG DPLQNTAGKR CGEAKRKLII 

      1090       1100       1110       1120       1130       1140 
SDLAEDPIHV TSHGLSLNID YYFSHLIGTA SVTFKALFGN DTKLTERLLK RFIPETRVVN 

      1150       1160       1170       1180       1190 
VKMLNRLQAA GFVCIHAPCW DNKMNTEAEI TEEEQSHQIM RRVFCIPKAI LHQS

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References

[1]"The complete DNA sequence of varicella-zoster virus."
Davison A.J., Scott J.E.
J. Gen. Virol. 67:1759-1816(1986) [PubMed: 3018124] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X04370 Genomic DNA. Translation: CAA27911.1.
PIRDJBE28. B27214.

3D structure databases

ProteinModelPortalP09252.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

ProtClustDBCLSP2511265.

Family and domain databases

InterProIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
Gene3DG3DSA:3.90.1600.10. DNA_pol_palm_dom. 2 hits.
PfamPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSPR00106. DNAPOLB.
SMARTSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. RNaseH_fold. 1 hit.
PROSITEPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

DrugBankDB00787. Aciclovir.
DB00426. Famciclovir.

Entry information

Entry nameDPOL_VZVD
AccessionPrimary (citable) accession number: P09252
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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