Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P09250 (KITH_VZVD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

EC=2.7.1.21
Gene names
ORF Names:ORF36
OrganismVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3) [Reference proteome]
Taxonomic identifier10338 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome By similarity.

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Subunit structure

Homodimer By similarity.

Miscellaneous

Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions By similarity.

Sequence similarities

Belongs to the herpesviridae thymidine kinase family.

Ontologies

Keywords
   Biological processDNA synthesis
   Developmental stageEarly protein
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

TMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Thymidine kinase
PRO_0000175084

Regions

Nucleotide binding19 – 268ATP Potential

Sites

Active site481Proton acceptor Potential
Binding site661Substrate By similarity
Binding site901Substrate By similarity
Binding site1391Substrate By similarity
Binding site1831ATP By similarity

Natural variations

Natural variant1301R → Q in strain: Isolate 7-1-3.
Natural variant1541L → P in strain: Isolate 40a2.
Natural variant2881S → L in strain: Ellen, ppIIa, Isolate clinical GK, Isolate 7-1-3, Isolate 101 and Isolate 3-5-2/KB3.
Natural variant3191A → V in strain: Isolate clinical GK.

Sequences

Sequence LengthMass (Da)Tools
P09250 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 1C95CC39750B0C07

FASTA34137,817
        10         20         30         40         50         60 
MSTDKTDVKM GVLRIYLDGA YGIGKTTAAE EFLHHFAITP NRILLIGEPL SYWRNLAGED 

        70         80         90        100        110        120 
AICGIYGTQT RRLNGDVSPE DAQRLTAHFQ SLFCSPHAIM HAKISALMDT STSDLVQVNK 

       130        140        150        160        170        180 
EPYKIMLSDR HPIASTICFP LSRYLVGDMS PAALPGLLFT LPAEPPGTNL VVCTVSLPSH 

       190        200        210        220        230        240 
LSRVSKRARP GETVNLPFVM VLRNVYIMLI NTIIFLKTNN WHAGWNTLSF CNDVFKQKLQ 

       250        260        270        280        290        300 
KSECIKLREV PGIEDTLFAV LKLPELCGEF GNILPLWAWG METLSNCSRS MSPFVLSLEQ 

       310        320        330        340 
TPQHAAQELK TLLPQMTPAN MSSGAWNILK ELVNAVQDNT S 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of varicella-zoster virus."
Davison A.J., Scott J.E.
J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Molecular analysis of the pyrimidine deoxyribonucleoside kinase gene of wild-type and acyclovir-resistant strains of varicella-zoster virus."
Sawyer M.H., Inchauspe G., Biron K.K., Waters D.J., Straus S.E., Ostrove J.M.
J. Gen. Virol. 69:2585-2593(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Ellen, Isolate 101, Isolate 3-5-2/KB3, Isolate 40a2, Isolate 7-1-3, isolate clinical GK and ppIIa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04370 Genomic DNA. Translation: CAA27919.1.
PIRKIBE36. A27341.
KIBEEL. A28930.
KIBE73. B28930.
KIBEGK. E28930.

3D structure databases

ProteinModelPortalP09250.
SMRP09250. Positions 8-338.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP09250.
ChEMBLCHEMBL4784.
DrugBankDB00194. Vidarabine.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKITH_VZVD
AccessionPrimary (citable) accession number: P09250
Secondary accession number(s): O57298 expand/collapse secondary AC list , P0C0E5, P0C0E7, P14341, P14342, P14343, P14344
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families