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P09250

- KITH_VZVD

UniProt

P09250 - KITH_VZVD

Protein

Thymidine kinase

Gene

ORF36

Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei48 – 481Proton acceptorSequence Analysis
    Binding sitei66 – 661SubstrateBy similarity
    Binding sitei90 – 901SubstrateBy similarity
    Binding sitei139 – 1391SubstrateBy similarity
    Binding sitei183 – 1831ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 268ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. TMP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    ORF Names:ORF36
    OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
    Taxonomic identifieri10338 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000002602: Genome

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341Thymidine kinasePRO_0000175084Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP09250.
    SMRiP09250. Positions 8-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR001889. Herpes_TK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00693. Herpes_TK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P09250-1 [UniParc]FASTAAdd to Basket

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    MSTDKTDVKM GVLRIYLDGA YGIGKTTAAE EFLHHFAITP NRILLIGEPL    50
    SYWRNLAGED AICGIYGTQT RRLNGDVSPE DAQRLTAHFQ SLFCSPHAIM 100
    HAKISALMDT STSDLVQVNK EPYKIMLSDR HPIASTICFP LSRYLVGDMS 150
    PAALPGLLFT LPAEPPGTNL VVCTVSLPSH LSRVSKRARP GETVNLPFVM 200
    VLRNVYIMLI NTIIFLKTNN WHAGWNTLSF CNDVFKQKLQ KSECIKLREV 250
    PGIEDTLFAV LKLPELCGEF GNILPLWAWG METLSNCSRS MSPFVLSLEQ 300
    TPQHAAQELK TLLPQMTPAN MSSGAWNILK ELVNAVQDNT S 341
    Length:341
    Mass (Da):37,817
    Last modified:July 1, 1989 - v1
    Checksum:i1C95CC39750B0C07
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti130 – 1301R → Q in strain: Isolate 7-1-3.
    Natural varianti154 – 1541L → P in strain: Isolate 40a2.
    Natural varianti288 – 2881S → L in strain: Ellen, ppIIa, Isolate clinical GK, Isolate 7-1-3, Isolate 101 and Isolate 3-5-2/KB3.
    Natural varianti319 – 3191A → V in strain: Isolate clinical GK.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04370 Genomic DNA. Translation: CAA27919.1.
    PIRiA27341. KIBE36.
    A28930. KIBEEL.
    B28930. KIBE73.
    E28930. KIBEGK.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04370 Genomic DNA. Translation: CAA27919.1 .
    PIRi A27341. KIBE36.
    A28930. KIBEEL.
    B28930. KIBE73.
    E28930. KIBEGK.

    3D structure databases

    ProteinModelPortali P09250.
    SMRi P09250. Positions 8-338.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P09250.
    ChEMBLi CHEMBL4784.
    DrugBanki DB00194. Vidarabine.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR001889. Herpes_TK.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00693. Herpes_TK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of varicella-zoster virus."
      Davison A.J., Scott J.E.
      J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Molecular analysis of the pyrimidine deoxyribonucleoside kinase gene of wild-type and acyclovir-resistant strains of varicella-zoster virus."
      Sawyer M.H., Inchauspe G., Biron K.K., Waters D.J., Straus S.E., Ostrove J.M.
      J. Gen. Virol. 69:2585-2593(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Ellen, Isolate 101, Isolate 3-5-2/KB3, Isolate 40a2, Isolate 7-1-3, isolate clinical GK and ppIIa.

    Entry informationi

    Entry nameiKITH_VZVD
    AccessioniPrimary (citable) accession number: P09250
    Secondary accession number(s): O57298
    , P0C0E5, P0C0E7, P14341, P14342, P14343, P14344
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3