Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidine kinase

Gene

ORF36

Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei48 – 481Proton acceptorSequence Analysis
Binding sitei66 – 661SubstrateBy similarity
Binding sitei90 – 901SubstrateBy similarity
Binding sitei139 – 1391SubstrateBy similarity
Binding sitei183 – 1831ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA biosynthetic process Source: UniProtKB-KW
  2. TMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
ORF Names:ORF36
OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri10338 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002602: Genome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Thymidine kinasePRO_0000175084Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP09250.
SMRiP09250. Positions 8-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P09250-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDKTDVKM GVLRIYLDGA YGIGKTTAAE EFLHHFAITP NRILLIGEPL
60 70 80 90 100
SYWRNLAGED AICGIYGTQT RRLNGDVSPE DAQRLTAHFQ SLFCSPHAIM
110 120 130 140 150
HAKISALMDT STSDLVQVNK EPYKIMLSDR HPIASTICFP LSRYLVGDMS
160 170 180 190 200
PAALPGLLFT LPAEPPGTNL VVCTVSLPSH LSRVSKRARP GETVNLPFVM
210 220 230 240 250
VLRNVYIMLI NTIIFLKTNN WHAGWNTLSF CNDVFKQKLQ KSECIKLREV
260 270 280 290 300
PGIEDTLFAV LKLPELCGEF GNILPLWAWG METLSNCSRS MSPFVLSLEQ
310 320 330 340
TPQHAAQELK TLLPQMTPAN MSSGAWNILK ELVNAVQDNT S
Length:341
Mass (Da):37,817
Last modified:July 1, 1989 - v1
Checksum:i1C95CC39750B0C07
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti130 – 1301R → Q in strain: Isolate 7-1-3.
Natural varianti154 – 1541L → P in strain: Isolate 40a2.
Natural varianti288 – 2881S → L in strain: Ellen, ppIIa, Isolate clinical GK, Isolate 7-1-3, Isolate 101 and Isolate 3-5-2/KB3.
Natural varianti319 – 3191A → V in strain: Isolate clinical GK.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27919.1.
PIRiA27341. KIBE36.
A28930. KIBEEL.
B28930. KIBE73.
E28930. KIBEGK.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27919.1.
PIRiA27341. KIBE36.
A28930. KIBEEL.
B28930. KIBE73.
E28930. KIBEGK.

3D structure databases

ProteinModelPortaliP09250.
SMRiP09250. Positions 8-338.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP09250.
ChEMBLiCHEMBL4784.
DrugBankiDB00194. Vidarabine.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of varicella-zoster virus."
    Davison A.J., Scott J.E.
    J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Molecular analysis of the pyrimidine deoxyribonucleoside kinase gene of wild-type and acyclovir-resistant strains of varicella-zoster virus."
    Sawyer M.H., Inchauspe G., Biron K.K., Waters D.J., Straus S.E., Ostrove J.M.
    J. Gen. Virol. 69:2585-2593(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Ellen, Isolate 101, Isolate 3-5-2/KB3, Isolate 40a2, Isolate 7-1-3, isolate clinical GK and ppIIa.

Entry informationi

Entry nameiKITH_VZVD
AccessioniPrimary (citable) accession number: P09250
Secondary accession number(s): O57298
, P0C0E5, P0C0E7, P14341, P14342, P14343, P14344
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 7, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.