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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei200SubstrateUniRule annotation1
Sitei216Important for hydrogen atom transferUniRule annotation1
Binding sitei246Substrate; via amide nitrogenUniRule annotation1
Active sitei427Proton acceptorUniRule annotation1
Active sitei429Cysteine radical intermediateUniRule annotation1
Active sitei431Proton acceptorUniRule annotation1
Sitei444Important for hydrogen atom transferUniRule annotation1
Sitei750Important for electron transferUniRule annotation1
Sitei751Important for electron transferUniRule annotation1
Sitei770Interacts with thioredoxin/glutaredoxinUniRule annotation1
Sitei773Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
Ordered Locus Names:ORF19
OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri10338 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000002602 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872451 – 775Ribonucleoside-diphosphate reductase large subunitAdd BLAST775

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi216 ↔ 444Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP09248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 216Substrate bindingUniRule annotation2
Regioni427 – 431Substrate bindingUniRule annotation5
Regioni606 – 610Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFKRIFNTV HDIINRLCQH GYKEYIIPPE STTPVELMEY ISTIVSKLKA
60 70 80 90 100
VTRQDERVYR CCGELIHCRI NLRSVSMETW LTSPILCLTP RVRQAIEGRR
110 120 130 140 150
DEIRRAILEP FLKDQYPALA TLGLQSALKY EDFYLTKLEE GKLESLCQFF
160 170 180 190 200
LRLAATVTTE IVNLPKIATL IPGINDGYTW TDVCRVFFTA LACQKIVPAT
210 220 230 240 250
PVMMFLGRET GATASCYLMD PESITVGRAV RAITGDVGTV LQSRGGVGIS
260 270 280 290 300
LQSLNLIPTE NQTKGLLAVL KLLDCMVMAI NSDCERPTGV CVYIEPWHVD
310 320 330 340 350
LQTVLATRGM LVRDEIFRCD NIFCCLWTPD LFFERYLSYL KGASNVQWTL
360 370 380 390 400
FDNRADILRT LHGEAFTSTY LRLEREGLGV SSVPIQDIAF TIIRSAAVTG
410 420 430 440 450
SPFLMFKDAC NRNYHMNTQG NAITGSNLCT EIVQKADAHQ HGVCNLASIN
460 470 480 490 500
LTTCLSKGPV SFNLNDLQLT ARTTVIFLNG VLAAGNFPCK KSCKGVKNNR
510 520 530 540 550
SLGIGIQGLH TTCLRLGFDL TSQPARRLNV QIAELMLYET MKTSMEMCKI
560 570 580 590 600
GGLAPFKGFT ESKYAKGWLH QDGFSTISYL DLPWCTLRDD ICAYGLYNSQ
610 620 630 640 650
FLALMPTVSS AQVTECSEGF SPIYNNMFSK VTTSGELLRP NLDLMDELRD
660 670 680 690 700
MYSCEEKRLE VINILEKNQW SVIRSFGCLS NSHPLLKYKT AFEYEQEDLV
710 720 730 740 750
DMCAERAPFI DQSQSMTLFI EERPDGTIPA SKIMNLLIRA YKAGLKTGMY
760 770
YCKIRKATNS GLFAGGELTC TSCAL
Length:775
Mass (Da):86,828
Last modified:July 1, 1989 - v1
Checksum:i187695A65EB91B9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27902.1.
PIRiA27343. WMBE19.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04370 Genomic DNA. Translation: CAA27902.1.
PIRiA27343. WMBE19.

3D structure databases

ProteinModelPortaliP09248.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_VZVD
AccessioniPrimary (citable) accession number: P09248
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.