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P09248

- RIR1_VZVD

UniProt

P09248 - RIR1_VZVD

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

ORF19

Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. The N-terminal region confers antiapoptotic activity in differentiated cells such as neurons and is important for viral reactivation to increase neural survivability By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei200 – 2001SubstrateBy similarity
    Sitei216 – 2161Important for hydrogen atom transferBy similarity
    Binding sitei246 – 2461Substrate; via amide nitrogenBy similarity
    Active sitei427 – 4271Proton acceptorBy similarity
    Active sitei429 – 4291Cysteine radical intermediateBy similarity
    Active sitei431 – 4311Proton acceptorBy similarity
    Sitei444 – 4441Important for hydrogen atom transferBy similarity
    Sitei750 – 7501Important for electron transferBy similarity
    Sitei751 – 7511Important for electron transferBy similarity
    Sitei770 – 7701Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei773 – 7731Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
    Short name:
    R1
    Alternative name(s):
    Ribonucleotide reductase large subunit
    Gene namesi
    ORF Names:ORF19
    OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
    Taxonomic identifieri10338 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000002602: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 775775Ribonucleoside-diphosphate reductase large subunitPRO_0000187245Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi216 ↔ 444Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit ORF19 (R1) and a homodimer of the small subunit ORF18 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP09248.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni215 – 2162Substrate bindingBy similarity
    Regioni427 – 4315Substrate bindingBy similarity
    Regioni606 – 6105Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view]
    PfamiPF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09248-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFKRIFNTV HDIINRLCQH GYKEYIIPPE STTPVELMEY ISTIVSKLKA    50
    VTRQDERVYR CCGELIHCRI NLRSVSMETW LTSPILCLTP RVRQAIEGRR 100
    DEIRRAILEP FLKDQYPALA TLGLQSALKY EDFYLTKLEE GKLESLCQFF 150
    LRLAATVTTE IVNLPKIATL IPGINDGYTW TDVCRVFFTA LACQKIVPAT 200
    PVMMFLGRET GATASCYLMD PESITVGRAV RAITGDVGTV LQSRGGVGIS 250
    LQSLNLIPTE NQTKGLLAVL KLLDCMVMAI NSDCERPTGV CVYIEPWHVD 300
    LQTVLATRGM LVRDEIFRCD NIFCCLWTPD LFFERYLSYL KGASNVQWTL 350
    FDNRADILRT LHGEAFTSTY LRLEREGLGV SSVPIQDIAF TIIRSAAVTG 400
    SPFLMFKDAC NRNYHMNTQG NAITGSNLCT EIVQKADAHQ HGVCNLASIN 450
    LTTCLSKGPV SFNLNDLQLT ARTTVIFLNG VLAAGNFPCK KSCKGVKNNR 500
    SLGIGIQGLH TTCLRLGFDL TSQPARRLNV QIAELMLYET MKTSMEMCKI 550
    GGLAPFKGFT ESKYAKGWLH QDGFSTISYL DLPWCTLRDD ICAYGLYNSQ 600
    FLALMPTVSS AQVTECSEGF SPIYNNMFSK VTTSGELLRP NLDLMDELRD 650
    MYSCEEKRLE VINILEKNQW SVIRSFGCLS NSHPLLKYKT AFEYEQEDLV 700
    DMCAERAPFI DQSQSMTLFI EERPDGTIPA SKIMNLLIRA YKAGLKTGMY 750
    YCKIRKATNS GLFAGGELTC TSCAL 775
    Length:775
    Mass (Da):86,828
    Last modified:July 1, 1989 - v1
    Checksum:i187695A65EB91B9D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04370 Genomic DNA. Translation: CAA27902.1.
    PIRiA27343. WMBE19.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04370 Genomic DNA. Translation: CAA27902.1 .
    PIRi A27343. WMBE19.

    3D structure databases

    ProteinModelPortali P09248.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view ]
    Pfami PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of varicella-zoster virus."
      Davison A.J., Scott J.E.
      J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Tinkering with a viral ribonucleotide reductase."
      Lembo D., Brune W.
      Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRIR1_VZVD
    AccessioniPrimary (citable) accession number: P09248
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3