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P09248

- RIR1_VZVD

UniProt

P09248 - RIR1_VZVD

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Protein
Ribonucleoside-diphosphate reductase large subunit
Gene
ORF19
Organism
Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. The N-terminal region confers antiapoptotic activity in differentiated cells such as neurons and is important for viral reactivation to increase neural survivability By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei200 – 2001Substrate By similarity
Sitei216 – 2161Important for hydrogen atom transfer By similarity
Binding sitei246 – 2461Substrate; via amide nitrogen By similarity
Active sitei427 – 4271Proton acceptor By similarity
Active sitei429 – 4291Cysteine radical intermediate By similarity
Active sitei431 – 4311Proton acceptor By similarity
Sitei444 – 4441Important for hydrogen atom transfer By similarity
Sitei750 – 7501Important for electron transfer By similarity
Sitei751 – 7511Important for electron transfer By similarity
Sitei770 – 7701Interacts with thioredoxin/glutaredoxin By similarity
Sitei773 – 7731Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Short name:
R1
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:ORF19
OrganismiVaricella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3)
Taxonomic identifieri10338 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000002602: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 775775Ribonucleoside-diphosphate reductase large subunit
PRO_0000187245Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi216 ↔ 444Redox-active By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit ORF19 (R1) and a homodimer of the small subunit ORF18 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliP09248.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni215 – 2162Substrate binding By similarity
Regioni427 – 4315Substrate binding By similarity
Regioni606 – 6105Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09248-1 [UniParc]FASTAAdd to Basket

« Hide

MEFKRIFNTV HDIINRLCQH GYKEYIIPPE STTPVELMEY ISTIVSKLKA    50
VTRQDERVYR CCGELIHCRI NLRSVSMETW LTSPILCLTP RVRQAIEGRR 100
DEIRRAILEP FLKDQYPALA TLGLQSALKY EDFYLTKLEE GKLESLCQFF 150
LRLAATVTTE IVNLPKIATL IPGINDGYTW TDVCRVFFTA LACQKIVPAT 200
PVMMFLGRET GATASCYLMD PESITVGRAV RAITGDVGTV LQSRGGVGIS 250
LQSLNLIPTE NQTKGLLAVL KLLDCMVMAI NSDCERPTGV CVYIEPWHVD 300
LQTVLATRGM LVRDEIFRCD NIFCCLWTPD LFFERYLSYL KGASNVQWTL 350
FDNRADILRT LHGEAFTSTY LRLEREGLGV SSVPIQDIAF TIIRSAAVTG 400
SPFLMFKDAC NRNYHMNTQG NAITGSNLCT EIVQKADAHQ HGVCNLASIN 450
LTTCLSKGPV SFNLNDLQLT ARTTVIFLNG VLAAGNFPCK KSCKGVKNNR 500
SLGIGIQGLH TTCLRLGFDL TSQPARRLNV QIAELMLYET MKTSMEMCKI 550
GGLAPFKGFT ESKYAKGWLH QDGFSTISYL DLPWCTLRDD ICAYGLYNSQ 600
FLALMPTVSS AQVTECSEGF SPIYNNMFSK VTTSGELLRP NLDLMDELRD 650
MYSCEEKRLE VINILEKNQW SVIRSFGCLS NSHPLLKYKT AFEYEQEDLV 700
DMCAERAPFI DQSQSMTLFI EERPDGTIPA SKIMNLLIRA YKAGLKTGMY 750
YCKIRKATNS GLFAGGELTC TSCAL 775
Length:775
Mass (Da):86,828
Last modified:July 1, 1989 - v1
Checksum:i187695A65EB91B9D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04370 Genomic DNA. Translation: CAA27902.1.
PIRiA27343. WMBE19.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04370 Genomic DNA. Translation: CAA27902.1 .
PIRi A27343. WMBE19.

3D structure databases

ProteinModelPortali P09248.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of varicella-zoster virus."
    Davison A.J., Scott J.E.
    J. Gen. Virol. 67:1759-1816(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_VZVD
AccessioniPrimary (citable) accession number: P09248
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 14, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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