Reviewed, UniProtKB/Swiss-Prot P09242 (PPBT_MOUSE)

Names and origin

Protein names

Recommended name:
    Alkaline phosphatase, tissue-nonspecific isozyme
    EC=3.1.3.1
Alternative name(s):
    AP-TNAP
    TNSALP

Gene names

Name:	Alpl
Synonyms:	Akp-2, Akp2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity.
Subunit structure	Homodimer.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Miscellaneous	In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from direct assay. Source: MGI
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MGI
Molecular function	alkaline phosphatase activity Inferred from direct assay. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17
	Chain	18 – 503	486	Alkaline phosphatase, tissue-nonspecific isozyme		PRO_0000024025
	Propeptide	504 – 524	21	Removed in mature form Potential		PRO_0000024026
Sites
	Active site	110	1	Phosphoserine intermediate
	Metal binding	60	1	Magnesium Potential
	Metal binding	60	1	Zinc 2 Potential
	Metal binding	173	1	Magnesium Potential
	Metal binding	332	1	Magnesium Potential
	Metal binding	337	1	Zinc 1 Potential
	Metal binding	341	1	Zinc 1 Potential
	Metal binding	378	1	Zinc 2 Potential
	Metal binding	379	1	Zinc 2 Potential
	Metal binding	454	1	Zinc 1 Potential
Amino acid modifications
	Lipidation	503	1	GPI-anchor amidated glycine Potential
	Glycosylation	140	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	230	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	430	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	439	1	N-linked (GlcNAc...) Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P09242-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 59D99113A60FA050	FASTA	524	57,455
	10 20 30 40 50 60 MISPFLVLAI GTCLTNSFVP EKERDPSYWR QQAQETLKNA LKLQKLNTNV AKNVIMFLGD 70 80 90 100 110 120 GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT YNTNAQVPDS AGTATAYLCG 130 140 150 160 170 180 VKANEGTVGV SAATERTRCN TTQGNEVTSI LRWAKDAGKS VGIVTTTRVN HATPSAAYAH 190 200 210 220 230 240 SADRDWYSDN EMPPEALSQG CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE 250 260 270 280 290 300 KARGTRLDGL DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN 310 320 330 340 350 360 RNNLTDPSLS EMVEVALRIL TKNLKGFFLL VEGGRIDHGH HEGKAKQALH EAVEMDQAIG 370 380 390 400 410 420 KAGAMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP MVSDTDKKPF TAILYGNGPG 430 440 450 460 470 480 YKVVDGEREN VSMVDYAHNN YQAQSAVPLR HETHGGEDVA VFAKGPMAHL LHGVHEQNYI 490 500 510 520 PHVMAYASCI GANLDHCAWA GSGSAPSPGA LLLPLAVLSL PTLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a cDNA coding for mouse placental alkaline phosphatase." Terao M., Mintz B. Proc. Natl. Acad. Sci. U.S.A. 84:7051-7055(1987) [PubMed: 3478679] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Induction of alkaline phosphatase in mouse L cells by overexpression of the catalytic subunit of cAMP-dependent protein kinase." Brown N.A., Stofko R.E., Uhler M.D. J. Biol. Chem. 265:13181-13189(1990) [PubMed: 2165496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-6.
[3]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140; ASN-230; ASN-303; ASN-430 AND ASN-439, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02980 mRNA. Translation: AAA39928.1. M54798 mRNA. Translation: AAA37217.1.
IPI	IPI00134743.
UniGene	Mm.288186
3D structure databases
HSSP	HSSP built from PDB template 1EW2 based on UniProtKB P05187.
ModBase	Search...
Protein-protein interaction databases
STRING	P09242.
Proteomic databases
PRIDE	P09242.
Genome annotation databases
Ensembl	ENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766; Mus musculus. [Genome view]
UCSC	uc008vjr.1. mouse.
Organism-specific databases
MGI	MGI:87983. Alpl.
Phylogenomic databases
HOGENOM	P09242.
HOVERGEN	P09242.
Enzyme and pathway databases
BRENDA	3.1.3.1. 244.
Gene expression databases
ArrayExpress	P09242.
Bgee	P09242.
CleanEx	MM_ALPL.
Genevestigator	P09242.
GermOnline	ENSMUSG00000028766. Mus musculus.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PPBT_MOUSE

Accession

Primary (citable) accession number: P09242

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	October 13, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)