Reviewed,
UniProtKB/Swiss-Prot P09242 (PPBT_MOUSE)
Last modified
October 13, 2009.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alkaline phosphatase, tissue-nonspecific isozyme EC=3.1.3.1 Alternative name(s): AP-TNAP TNSALP | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 524 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | A phosphate monoester + H2O = an alcohol + phosphate. |
| Cofactor | Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity. |
| Subunit structure | Homodimer. |
| Subcellular location | |
| Miscellaneous | In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). |
| Sequence similarities | Belongs to the alkaline phosphatase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Domain | Signal |
| Ligand | Magnesium Metal-binding Zinc |
| Molecular function | Hydrolase |
| PTM | GPI-anchor Glycoprotein Lipoprotein Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | metabolic process Inferred from electronic annotation. Source: InterPro response to antibioticInferred from direct assay. Source: MGI |
| Cellular component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membraneInferred from direct assay. Source: MGI |
| Molecular function | alkaline phosphatase activity Inferred from direct assay. Source: MGI magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 17 | 17 | |||||||
| Chain | 18 – 503 | 486 | Alkaline phosphatase, tissue-nonspecific isozyme | PRO_0000024025 | |||||
| Propeptide | 504 – 524 | 21 | Removed in mature form Potential | PRO_0000024026 | |||||
Sites | |||||||||
| Active site | 110 | 1 | Phosphoserine intermediate | ||||||
| Metal binding | 60 | 1 | Magnesium Potential | ||||||
| Metal binding | 60 | 1 | Zinc 2 Potential | ||||||
| Metal binding | 173 | 1 | Magnesium Potential | ||||||
| Metal binding | 332 | 1 | Magnesium Potential | ||||||
| Metal binding | 337 | 1 | Zinc 1 Potential | ||||||
| Metal binding | 341 | 1 | Zinc 1 Potential | ||||||
| Metal binding | 378 | 1 | Zinc 2 Potential | ||||||
| Metal binding | 379 | 1 | Zinc 2 Potential | ||||||
| Metal binding | 454 | 1 | Zinc 1 Potential | ||||||
Amino acid modifications | |||||||||
| Lipidation | 503 | 1 | GPI-anchor amidated glycine Potential | ||||||
| Glycosylation | 140 | 1 | N-linked (GlcNAc...) Ref.3 | ||||||
| Glycosylation | 230 | 1 | N-linked (GlcNAc...) Ref.3 | ||||||
| Glycosylation | 271 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 303 | 1 | N-linked (GlcNAc...) Ref.3 | ||||||
| Glycosylation | 430 | 1 | N-linked (GlcNAc...) Ref.3 | ||||||
| Glycosylation | 439 | 1 | N-linked (GlcNAc...) Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of a cDNA coding for mouse placental alkaline phosphatase." Terao M., Mintz B. Proc. Natl. Acad. Sci. U.S.A. 84:7051-7055(1987) [PubMed: 3478679] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta. |
| [2] | "Induction of alkaline phosphatase in mouse L cells by overexpression of the catalytic subunit of cAMP-dependent protein kinase." Brown N.A., Stofko R.E., Uhler M.D. J. Biol. Chem. 265:13181-13189(1990) [PubMed: 2165496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-6. |
| [3] | "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140; ASN-230; ASN-303; ASN-430 AND ASN-439, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| J02980 mRNA. Translation: AAA39928.1. M54798 mRNA. Translation: AAA37217.1. | |
| IPI | IPI00134743. |
| UniGene | Mm.288186 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1EW2 based on UniProtKB P05187. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P09242. |
Proteomic databases | |
| PRIDE | P09242. |
Genome annotation databases | |
| Ensembl | ENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766; Mus musculus. [Genome view] |
| UCSC | uc008vjr.1. mouse. |
Organism-specific databases | |
| MGI | MGI:87983. Alpl. |
Phylogenomic databases | |
| HOGENOM | P09242. |
| HOVERGEN | P09242. |
Enzyme and pathway databases | |
| BRENDA | 3.1.3.1. 244. |
Gene expression databases | |
| ArrayExpress | P09242. |
| Bgee | P09242. |
| CleanEx | MM_ALPL. |
| Genevestigator | P09242. |
| GermOnline | ENSMUSG00000028766. Mus musculus. |
Family and domain databases | |
| InterPro | IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. [Graphical view] |
| Gene3D | G3DSA:3.40.720.10. Alk_phosphtse. 1 hit. |
| Pfam | PF00245. Alk_phosphatase. 1 hit. [Graphical view] |
| PRINTS | PR00113. ALKPHPHTASE. |
| SMART | SM00098. alkPPc. 1 hit. [Graphical view] |
| PROSITE | PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | PPBT_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P09242 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


