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P09242

- PPBT_MOUSE

UniProt

P09242 - PPBT_MOUSE

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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Binds 1 magnesium ion.By similarity
Binds 2 zinc ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601MagnesiumBy similarity
Metal bindingi60 – 601Zinc 1By similarity
Active sitei110 – 1101Phosphoserine intermediate
Metal bindingi110 – 1101Zinc 1By similarity
Metal bindingi173 – 1731MagnesiumBy similarity
Metal bindingi332 – 3321MagnesiumBy similarity
Metal bindingi337 – 3371Zinc 2By similarity
Metal bindingi341 – 3411Zinc 2; via tele nitrogenBy similarity
Metal bindingi378 – 3781Zinc 1By similarity
Metal bindingi379 – 3791Zinc 1; via tele nitrogenBy similarity
Metal bindingi454 – 4541Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. pyrophosphatase activity Source: MGI

GO - Biological processi

  1. cellular response to organic cyclic compound Source: MGI
  2. cementum mineralization Source: Ensembl
  3. developmental process involved in reproduction Source: MGI
  4. endochondral ossification Source: MGI
  5. osteoblast differentiation Source: Ensembl
  6. response to antibiotic Source: MGI
  7. response to glucocorticoid Source: Ensembl
  8. response to lipopolysaccharide Source: Ensembl
  9. response to vitamin D Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP09242.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase 2
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:Alpl
Synonyms:Akp-2, Akp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:87983. Alpl.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular matrix Source: MGI
  3. extracellular membrane-bounded organelle Source: MGI
  4. extracellular space Source: Ensembl
  5. extracellular vesicular exosome Source: Ensembl
  6. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Add
BLAST
Chaini18 – 503486Alkaline phosphatase, tissue-nonspecific isozymePRO_0000024025Add
BLAST
Propeptidei504 – 52421Removed in mature formSequence AnalysisPRO_0000024026Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140 – 1401N-linked (GlcNAc...)1 Publication
Glycosylationi230 – 2301N-linked (GlcNAc...)1 Publication
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)1 Publication
Glycosylationi430 – 4301N-linked (GlcNAc...)1 Publication
Glycosylationi439 – 4391N-linked (GlcNAc...); atypical1 Publication
Disulfide bondi489 ↔ 497By similarity
Lipidationi503 – 5031GPI-anchor amidated glycineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP09242.
PaxDbiP09242.
PRIDEiP09242.

PTM databases

PhosphoSiteiP09242.

Expressioni

Gene expression databases

BgeeiP09242.
CleanExiMM_ALPL.
ExpressionAtlasiP09242. baseline and differential.
GenevestigatoriP09242.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi198053. 1 interaction.
IntActiP09242. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP09242.
SMRiP09242. Positions 19-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09242.
KOiK01077.
OMAiRGNCAST.
OrthoDBiEOG7SN8CH.
TreeFamiTF323513.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09242-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISPFLVLAI GTCLTNSFVP EKERDPSYWR QQAQETLKNA LKLQKLNTNV
60 70 80 90 100
AKNVIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNLTDPSLS EMVEVALRIL TKNLKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDQAIG KAGAMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA
510 520
GSGSAPSPGA LLLPLAVLSL RTLF
Length:524
Mass (Da):57,514
Last modified:July 27, 2011 - v2
Checksum:i59D99110C60FA050
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti521 – 5211R → P in AAA39928. (PubMed:3478679)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02980 mRNA. Translation: AAA39928.1.
AK161780 mRNA. Translation: BAE36569.1.
AK167023 mRNA. Translation: BAE39196.1.
AL805954, AL807764 Genomic DNA. Translation: CAM19406.1.
AL807764, AL805954 Genomic DNA. Translation: CAM23370.1.
BC065175 mRNA. Translation: AAH65175.1.
M54798 mRNA. Translation: AAA37217.1.
CCDSiCCDS18821.1.
RefSeqiNP_001274101.1. NM_001287172.1.
NP_001274105.1. NM_001287176.1.
NP_031457.2. NM_007431.3.
XP_006538560.1. XM_006538497.1.
XP_006538561.1. XM_006538498.1.
XP_006538562.1. XM_006538499.1.
XP_006538563.1. XM_006538500.1.
UniGeneiMm.288186.

Genome annotation databases

EnsembliENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766.
GeneIDi11647.
KEGGimmu:11647.
UCSCiuc008vjr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02980 mRNA. Translation: AAA39928.1 .
AK161780 mRNA. Translation: BAE36569.1 .
AK167023 mRNA. Translation: BAE39196.1 .
AL805954 , AL807764 Genomic DNA. Translation: CAM19406.1 .
AL807764 , AL805954 Genomic DNA. Translation: CAM23370.1 .
BC065175 mRNA. Translation: AAH65175.1 .
M54798 mRNA. Translation: AAA37217.1 .
CCDSi CCDS18821.1.
RefSeqi NP_001274101.1. NM_001287172.1.
NP_001274105.1. NM_001287176.1.
NP_031457.2. NM_007431.3.
XP_006538560.1. XM_006538497.1.
XP_006538561.1. XM_006538498.1.
XP_006538562.1. XM_006538499.1.
XP_006538563.1. XM_006538500.1.
UniGenei Mm.288186.

3D structure databases

ProteinModelPortali P09242.
SMRi P09242. Positions 19-490.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198053. 1 interaction.
IntActi P09242. 1 interaction.

Chemistry

ChEMBLi CHEMBL2660.

PTM databases

PhosphoSitei P09242.

Proteomic databases

MaxQBi P09242.
PaxDbi P09242.
PRIDEi P09242.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030551 ; ENSMUSP00000030551 ; ENSMUSG00000028766 .
GeneIDi 11647.
KEGGi mmu:11647.
UCSCi uc008vjr.2. mouse.

Organism-specific databases

CTDi 249.
MGIi MGI:87983. Alpl.

Phylogenomic databases

eggNOGi COG1785.
GeneTreei ENSGT00390000008704.
HOGENOMi HOG000099118.
HOVERGENi HBG007345.
InParanoidi P09242.
KOi K01077.
OMAi RGNCAST.
OrthoDBi EOG7SN8CH.
TreeFami TF323513.

Enzyme and pathway databases

SABIO-RK P09242.

Miscellaneous databases

NextBioi 279245.
PROi P09242.
SOURCEi Search...

Gene expression databases

Bgeei P09242.
CleanExi MM_ALPL.
ExpressionAtlasi P09242. baseline and differential.
Genevestigatori P09242.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a cDNA coding for mouse placental alkaline phosphatase."
    Terao M., Mintz B.
    Proc. Natl. Acad. Sci. U.S.A. 84:7051-7055(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Forelimb.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Induction of alkaline phosphatase in mouse L cells by overexpression of the catalytic subunit of cAMP-dependent protein kinase."
    Brown N.A., Stofko R.E., Uhler M.D.
    J. Biol. Chem. 265:13181-13189(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-6.
  6. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140; ASN-230; ASN-303; ASN-430 AND ASN-439.

Entry informationi

Entry nameiPPBT_MOUSE
AccessioniPrimary (citable) accession number: P09242
Secondary accession number(s): Q6P1B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3