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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This isozyme may play a role in skeletal mineralization.By similarity

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotationBy similarity

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60MagnesiumBy similarity1
Metal bindingi60Zinc 1By similarity1
Active sitei110Phosphoserine intermediateBy similarity1
Metal bindingi110Zinc 1By similarity1
Metal bindingi173MagnesiumBy similarity1
Metal bindingi332MagnesiumBy similarity1
Metal bindingi337Zinc 2By similarity1
Metal bindingi341Zinc 2; via tele nitrogenBy similarity1
Metal bindingi378Zinc 1By similarity1
Metal bindingi379Zinc 1; via tele nitrogenBy similarity1
Metal bindingi454Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • pyrophosphatase activity Source: MGI

GO - Biological processi

  • cellular response to organic cyclic compound Source: MGI
  • cementum mineralization Source: Ensembl
  • developmental process involved in reproduction Source: MGI
  • endochondral ossification Source: MGI
  • osteoblast differentiation Source: MGI
  • response to antibiotic Source: MGI
  • response to glucocorticoid Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • response to vitamin D Source: Ensembl

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1. 3474.
ReactomeiR-MMU-163125. Post-translational modification: synthesis of GPI-anchored proteins.
SABIO-RKiP09242.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1By similarity)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase 2
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:Alpl
Synonyms:Akp-2, Akp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:87983. Alpl.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2660.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Add BLAST17
ChainiPRO_000002402518 – 501Alkaline phosphatase, tissue-nonspecific isozymeAdd BLAST484
PropeptideiPRO_0000024026502 – 524Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi230N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi430N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi439N-linked (GlcNAc...) asparagine; atypical1 Publication1
Disulfide bondi489 ↔ 497By similarity
Lipidationi501GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP09242.
PaxDbiP09242.
PeptideAtlasiP09242.
PRIDEiP09242.

PTM databases

iPTMnetiP09242.
PhosphoSitePlusiP09242.
SwissPalmiP09242.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028766.
CleanExiMM_ALPL.
ExpressionAtlasiP09242. baseline and differential.
GenevisibleiP09242. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi198053. 3 interactors.
IntActiP09242. 1 interactor.
STRINGi10090.ENSMUSP00000030551.

Structurei

3D structure databases

ProteinModelPortaliP09242.
SMRiP09242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09242.
KOiK01077.
OMAiEHTGTQL.
OrthoDBiEOG091G067H.
TreeFamiTF323513.

Family and domain databases

CDDicd16012. ALP. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
PANTHERiPTHR11596. PTHR11596. 1 hit.
PfamiView protein in Pfam
PF00245. Alk_phosphatase. 1 hit.
PRINTSiPR00113. ALKPHPHTASE.
SMARTiView protein in SMART
SM00098. alkPPc. 1 hit.
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiView protein in PROSITE
PS00123. ALKALINE_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISPFLVLAI GTCLTNSFVP EKERDPSYWR QQAQETLKNA LKLQKLNTNV
60 70 80 90 100
AKNVIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNLTDPSLS EMVEVALRIL TKNLKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDQAIG KAGAMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA
510 520
GSGSAPSPGA LLLPLAVLSL RTLF
Length:524
Mass (Da):57,514
Last modified:July 27, 2011 - v2
Checksum:i59D99110C60FA050
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti521R → P in AAA39928 (PubMed:3478679).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02980 mRNA. Translation: AAA39928.1.
AK161780 mRNA. Translation: BAE36569.1.
AK167023 mRNA. Translation: BAE39196.1.
AL805954, AL807764 Genomic DNA. Translation: CAM19406.1.
AL807764, AL805954 Genomic DNA. Translation: CAM23370.1.
BC065175 mRNA. Translation: AAH65175.1.
M54798 mRNA. Translation: AAA37217.1.
CCDSiCCDS18821.1.
RefSeqiNP_001274101.1. NM_001287172.1.
NP_031457.2. NM_007431.3.
XP_006538560.1. XM_006538497.3.
XP_006538561.1. XM_006538498.3.
XP_006538562.1. XM_006538499.3.
XP_006538563.1. XM_006538500.2.
XP_017175413.1. XM_017319924.1.
UniGeneiMm.288186.

Genome annotation databases

EnsembliENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766.
GeneIDi11647.
KEGGimmu:11647.
UCSCiuc008vjr.3. mouse.

Similar proteinsi

Entry informationi

Entry nameiPPBT_MOUSE
AccessioniPrimary (citable) accession number: P09242
Secondary accession number(s): Q6P1B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: September 27, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families