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P09242

- PPBT_MOUSE

UniProt

P09242 - PPBT_MOUSE

Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601MagnesiumBy similarity
    Metal bindingi60 – 601Zinc 1By similarity
    Active sitei110 – 1101Phosphoserine intermediate
    Metal bindingi110 – 1101Zinc 1By similarity
    Metal bindingi173 – 1731MagnesiumBy similarity
    Metal bindingi332 – 3321MagnesiumBy similarity
    Metal bindingi337 – 3371Zinc 2By similarity
    Metal bindingi341 – 3411Zinc 2; via tele nitrogenBy similarity
    Metal bindingi378 – 3781Zinc 1By similarity
    Metal bindingi379 – 3791Zinc 1; via tele nitrogenBy similarity
    Metal bindingi454 – 4541Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. pyrophosphatase activity Source: MGI

    GO - Biological processi

    1. cellular response to organic cyclic compound Source: MGI
    2. cementum mineralization Source: Ensembl
    3. developmental process involved in reproduction Source: MGI
    4. endochondral ossification Source: MGI
    5. response to antibiotic Source: MGI
    6. response to glucocorticoid Source: Ensembl
    7. response to lipopolysaccharide Source: Ensembl
    8. response to vitamin D Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP09242.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1)
    Short name:
    AP-TNAP
    Short name:
    TNSALP
    Alternative name(s):
    Alkaline phosphatase 2
    Alkaline phosphatase liver/bone/kidney isozyme
    Gene namesi
    Name:Alpl
    Synonyms:Akp-2, Akp2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:87983. Alpl.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular matrix Source: MGI
    3. extracellular membrane-bounded organelle Source: MGI
    4. extracellular space Source: Ensembl
    5. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Add
    BLAST
    Chaini18 – 503486Alkaline phosphatase, tissue-nonspecific isozymePRO_0000024025Add
    BLAST
    Propeptidei504 – 52421Removed in mature formSequence AnalysisPRO_0000024026Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi139 ↔ 201By similarity
    Glycosylationi140 – 1401N-linked (GlcNAc...)1 Publication
    Glycosylationi230 – 2301N-linked (GlcNAc...)1 Publication
    Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)1 Publication
    Glycosylationi430 – 4301N-linked (GlcNAc...)1 Publication
    Glycosylationi439 – 4391N-linked (GlcNAc...); atypical1 Publication
    Disulfide bondi489 ↔ 497By similarity
    Lipidationi503 – 5031GPI-anchor amidated glycineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP09242.
    PaxDbiP09242.
    PRIDEiP09242.

    PTM databases

    PhosphoSiteiP09242.

    Expressioni

    Gene expression databases

    ArrayExpressiP09242.
    BgeeiP09242.
    CleanExiMM_ALPL.
    GenevestigatoriP09242.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi198053. 1 interaction.
    IntActiP09242. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP09242.
    SMRiP09242. Positions 19-490.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    GeneTreeiENSGT00390000008704.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    InParanoidiQ6P1B0.
    KOiK01077.
    OMAiRGNCAST.
    OrthoDBiEOG7SN8CH.
    TreeFamiTF323513.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09242-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISPFLVLAI GTCLTNSFVP EKERDPSYWR QQAQETLKNA LKLQKLNTNV    50
    AKNVIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT 100
    YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI 150
    LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG 200
    CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL 250
    DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN 300
    RNNLTDPSLS EMVEVALRIL TKNLKGFFLL VEGGRIDHGH HEGKAKQALH 350
    EAVEMDQAIG KAGAMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP 400
    MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR 450
    HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA 500
    GSGSAPSPGA LLLPLAVLSL RTLF 524
    Length:524
    Mass (Da):57,514
    Last modified:July 27, 2011 - v2
    Checksum:i59D99110C60FA050
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti521 – 5211R → P in AAA39928. (PubMed:3478679)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02980 mRNA. Translation: AAA39928.1.
    AK161780 mRNA. Translation: BAE36569.1.
    AK167023 mRNA. Translation: BAE39196.1.
    AL805954, AL807764 Genomic DNA. Translation: CAM19406.1.
    AL807764, AL805954 Genomic DNA. Translation: CAM23370.1.
    BC065175 mRNA. Translation: AAH65175.1.
    M54798 mRNA. Translation: AAA37217.1.
    CCDSiCCDS18821.1.
    RefSeqiNP_001274101.1. NM_001287172.1.
    NP_001274105.1. NM_001287176.1.
    NP_031457.2. NM_007431.3.
    XP_006538560.1. XM_006538497.1.
    XP_006538561.1. XM_006538498.1.
    XP_006538562.1. XM_006538499.1.
    XP_006538563.1. XM_006538500.1.
    UniGeneiMm.288186.

    Genome annotation databases

    EnsembliENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766.
    GeneIDi11647.
    KEGGimmu:11647.
    UCSCiuc008vjr.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02980 mRNA. Translation: AAA39928.1 .
    AK161780 mRNA. Translation: BAE36569.1 .
    AK167023 mRNA. Translation: BAE39196.1 .
    AL805954 , AL807764 Genomic DNA. Translation: CAM19406.1 .
    AL807764 , AL805954 Genomic DNA. Translation: CAM23370.1 .
    BC065175 mRNA. Translation: AAH65175.1 .
    M54798 mRNA. Translation: AAA37217.1 .
    CCDSi CCDS18821.1.
    RefSeqi NP_001274101.1. NM_001287172.1.
    NP_001274105.1. NM_001287176.1.
    NP_031457.2. NM_007431.3.
    XP_006538560.1. XM_006538497.1.
    XP_006538561.1. XM_006538498.1.
    XP_006538562.1. XM_006538499.1.
    XP_006538563.1. XM_006538500.1.
    UniGenei Mm.288186.

    3D structure databases

    ProteinModelPortali P09242.
    SMRi P09242. Positions 19-490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198053. 1 interaction.
    IntActi P09242. 1 interaction.

    Chemistry

    ChEMBLi CHEMBL2660.

    PTM databases

    PhosphoSitei P09242.

    Proteomic databases

    MaxQBi P09242.
    PaxDbi P09242.
    PRIDEi P09242.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030551 ; ENSMUSP00000030551 ; ENSMUSG00000028766 .
    GeneIDi 11647.
    KEGGi mmu:11647.
    UCSCi uc008vjr.2. mouse.

    Organism-specific databases

    CTDi 249.
    MGIi MGI:87983. Alpl.

    Phylogenomic databases

    eggNOGi COG1785.
    GeneTreei ENSGT00390000008704.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    InParanoidi Q6P1B0.
    KOi K01077.
    OMAi RGNCAST.
    OrthoDBi EOG7SN8CH.
    TreeFami TF323513.

    Enzyme and pathway databases

    SABIO-RK P09242.

    Miscellaneous databases

    NextBioi 279245.
    PROi P09242.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P09242.
    Bgeei P09242.
    CleanExi MM_ALPL.
    Genevestigatori P09242.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a cDNA coding for mouse placental alkaline phosphatase."
      Terao M., Mintz B.
      Proc. Natl. Acad. Sci. U.S.A. 84:7051-7055(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Placenta.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Forelimb.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    5. "Induction of alkaline phosphatase in mouse L cells by overexpression of the catalytic subunit of cAMP-dependent protein kinase."
      Brown N.A., Stofko R.E., Uhler M.D.
      J. Biol. Chem. 265:13181-13189(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-6.
    6. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140; ASN-230; ASN-303; ASN-430 AND ASN-439.

    Entry informationi

    Entry nameiPPBT_MOUSE
    AccessioniPrimary (citable) accession number: P09242
    Secondary accession number(s): Q6P1B0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3