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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

Alpl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This isozyme may play a role in skeletal mineralization.By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotationBy similarity

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60MagnesiumBy similarity1
Metal bindingi60Zinc 1By similarity1
Active sitei110Phosphoserine intermediateBy similarity1
Metal bindingi110Zinc 1By similarity1
Metal bindingi173MagnesiumBy similarity1
Metal bindingi332MagnesiumBy similarity1
Metal bindingi337Zinc 2By similarity1
Metal bindingi341Zinc 2; via tele nitrogenBy similarity1
Metal bindingi378Zinc 1By similarity1
Metal bindingi379Zinc 1; via tele nitrogenBy similarity1
Metal bindingi454Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • pyrophosphatase activity Source: MGI

GO - Biological processi

  • cellular response to organic cyclic compound Source: MGI
  • cementum mineralization Source: Ensembl
  • developmental process involved in reproduction Source: MGI
  • endochondral ossification Source: MGI
  • osteoblast differentiation Source: MGI
  • response to antibiotic Source: MGI
  • response to glucocorticoid Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • response to vitamin D Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1. 3474.
SABIO-RKP09242.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1By similarity)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase 2
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:Alpl
Synonyms:Akp-2, Akp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:87983. Alpl.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular exosome Source: MGI
  • extracellular membrane-bounded organelle Source: MGI
  • extracellular space Source: Ensembl
  • membrane Source: MGI
  • plasma membrane Source: MGI
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2660.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Add BLAST17
ChainiPRO_000002402518 – 503Alkaline phosphatase, tissue-nonspecific isozymeAdd BLAST486
PropeptideiPRO_0000024026504 – 524Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140N-linked (GlcNAc...)1 Publication1
Glycosylationi230N-linked (GlcNAc...)1 Publication1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi303N-linked (GlcNAc...)1 Publication1
Glycosylationi430N-linked (GlcNAc...)1 Publication1
Glycosylationi439N-linked (GlcNAc...); atypical1 Publication1
Disulfide bondi489 ↔ 497By similarity
Lipidationi503GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

MaxQBiP09242.
PaxDbiP09242.
PeptideAtlasiP09242.
PRIDEiP09242.

PTM databases

iPTMnetiP09242.
PhosphoSitePlusiP09242.
SwissPalmiP09242.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028766.
CleanExiMM_ALPL.
ExpressionAtlasiP09242. baseline and differential.
GenevisibleiP09242. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi198053. 2 interactors.
IntActiP09242. 1 interactor.
STRINGi10090.ENSMUSP00000030551.

Structurei

3D structure databases

ProteinModelPortaliP09242.
SMRiP09242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09242.
KOiK01077.
OMAiYTYVTTG.
OrthoDBiEOG091G067H.
TreeFamiTF323513.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISPFLVLAI GTCLTNSFVP EKERDPSYWR QQAQETLKNA LKLQKLNTNV
60 70 80 90 100
AKNVIMFLGD GMGVSTVTAA RILKGQLHHN TGEETRLEMD KFPFVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERTRCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIDVIMG GGRKYMYPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
DLISIWKSFK PRHKHSHYVW NRTELLALDP SRVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNLTDPSLS EMVEVALRIL TKNLKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDQAIG KAGAMTSQKD TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVDGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYASCI GANLDHCAWA
510 520
GSGSAPSPGA LLLPLAVLSL RTLF
Length:524
Mass (Da):57,514
Last modified:July 27, 2011 - v2
Checksum:i59D99110C60FA050
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti521R → P in AAA39928 (PubMed:3478679).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02980 mRNA. Translation: AAA39928.1.
AK161780 mRNA. Translation: BAE36569.1.
AK167023 mRNA. Translation: BAE39196.1.
AL805954, AL807764 Genomic DNA. Translation: CAM19406.1.
AL807764, AL805954 Genomic DNA. Translation: CAM23370.1.
BC065175 mRNA. Translation: AAH65175.1.
M54798 mRNA. Translation: AAA37217.1.
CCDSiCCDS18821.1.
RefSeqiNP_001274101.1. NM_001287172.1.
NP_031457.2. NM_007431.3.
XP_006538560.1. XM_006538497.3.
XP_006538561.1. XM_006538498.3.
XP_006538562.1. XM_006538499.3.
XP_006538563.1. XM_006538500.2.
XP_017175413.1. XM_017319924.1.
UniGeneiMm.288186.

Genome annotation databases

EnsembliENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766.
GeneIDi11647.
KEGGimmu:11647.
UCSCiuc008vjr.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02980 mRNA. Translation: AAA39928.1.
AK161780 mRNA. Translation: BAE36569.1.
AK167023 mRNA. Translation: BAE39196.1.
AL805954, AL807764 Genomic DNA. Translation: CAM19406.1.
AL807764, AL805954 Genomic DNA. Translation: CAM23370.1.
BC065175 mRNA. Translation: AAH65175.1.
M54798 mRNA. Translation: AAA37217.1.
CCDSiCCDS18821.1.
RefSeqiNP_001274101.1. NM_001287172.1.
NP_031457.2. NM_007431.3.
XP_006538560.1. XM_006538497.3.
XP_006538561.1. XM_006538498.3.
XP_006538562.1. XM_006538499.3.
XP_006538563.1. XM_006538500.2.
XP_017175413.1. XM_017319924.1.
UniGeneiMm.288186.

3D structure databases

ProteinModelPortaliP09242.
SMRiP09242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198053. 2 interactors.
IntActiP09242. 1 interactor.
STRINGi10090.ENSMUSP00000030551.

Chemistry databases

ChEMBLiCHEMBL2660.

PTM databases

iPTMnetiP09242.
PhosphoSitePlusiP09242.
SwissPalmiP09242.

Proteomic databases

MaxQBiP09242.
PaxDbiP09242.
PeptideAtlasiP09242.
PRIDEiP09242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030551; ENSMUSP00000030551; ENSMUSG00000028766.
GeneIDi11647.
KEGGimmu:11647.
UCSCiuc008vjr.3. mouse.

Organism-specific databases

CTDi249.
MGIiMGI:87983. Alpl.

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09242.
KOiK01077.
OMAiYTYVTTG.
OrthoDBiEOG091G067H.
TreeFamiTF323513.

Enzyme and pathway databases

BRENDAi3.1.3.1. 3474.
SABIO-RKP09242.

Miscellaneous databases

PROiP09242.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028766.
CleanExiMM_ALPL.
ExpressionAtlasiP09242. baseline and differential.
GenevisibleiP09242. MM.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPBT_MOUSE
AccessioniPrimary (citable) accession number: P09242
Secondary accession number(s): Q6P1B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.