P09240 (CCKN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholecystokinin
Short name=CCK

Cleaved into the following 3 chains:

1. Cholecystokinin-33
   Short name=CCK33
2. Cholecystokinin-12
   Short name=CCK12
3. Cholecystokinin-8
   Short name=CCK8

Gene names

Name:

Cck

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	115 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Subunit structure	Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain By similarity.
Subcellular location	Secreted.
Post-translational modification	The precursor is cleaved by proteases to produce a number of active cholecystokinins.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara axonogenesis Inferred from direct assay PubMed 15152034. Source: MGI behavioral fear response Inferred from electronic annotation. Source: Compara eating behavior Inferred from direct assay PubMed 15374756. Source: MGI negative regulation of appetite Inferred from electronic annotation. Source: Compara neuron migration Inferred from direct assay PubMed 15152034. Source: MGI positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of mitochondrial depolarization Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of protein oligomerization Inferred from electronic annotation. Source: Compara protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara regulation of sensory perception of pain Inferred from electronic annotation. Source: Compara release of cytochrome c from mitochondria Inferred from electronic annotation. Source: Compara
Cellular_component	axon Inferred from direct assay PubMed 15152034. Source: MGI axon hillock Inferred from electronic annotation. Source: Compara axon initial segment Inferred from electronic annotation. Source: Compara dendrite Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara perikaryon Inferred from electronic annotation. Source: Compara terminal bouton Inferred from electronic annotation. Source: Compara
Molecular_function	neuropeptide hormone activity Inferred from direct assay PubMed 15374756. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 115	95	Cholecystokinin		PRO_0000010552
	Propeptide	21 – 70	50			PRO_0000010553
	Peptide	71 – 103	33	Cholecystokinin-33		PRO_0000010554
	Peptide	92 – 103	12	Cholecystokinin-12		PRO_0000010555
	Peptide	96 – 103	8	Cholecystokinin-8		PRO_0000010556
	Propeptide	107 – 115	9			PRO_0000010557
Amino acid modifications
	Modified residue	97	1	Sulfotyrosine By similarity
	Modified residue	103	1	Phenylalanine amide
	Modified residue	111	1	Sulfotyrosine By similarity
	Modified residue	113	1	Sulfotyrosine By similarity
Experimental info
	Sequence conflict	37	1	Q → E in CAB94216. Ref.1
	Sequence conflict	50	1	T → P in CAB94216. Ref.1
	Sequence conflict	52	1	G → R in CAB94216. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P09240 [UniParc]. Last modified April 8, 2008. Version 3. Checksum: 7841B11D39BB52DA	FASTA	115	12,770
	10 20 30 40 50 60 MKSGVCLCVV MAVLAAGALA QPVVPAEATD PVEQRAQEAP RRQLRAVLRT DGEPRARLGA 70 80 90 100 110 LLARYIQQVR KAPSGRMSVL KNLQSLDPSH RISDRDYMGW MDFGRRSAED YEYPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the mouse CCK gene: expression in different brain regions and during cortical development." Vitale M., Vashishtha A., Linzer E., Powell D.J., Friedman J.M. Nucleic Acids Res. 19:169-177(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/3J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney.
[3]	"Differential expression of the mouse cholecystokinin gene during brain and gut development." Friedman J.M., Schneider B.S., Powell D.J. Proc. Natl. Acad. Sci. U.S.A. 82:5593-5597(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 55-115.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59521, X59522 Genomic DNA. Translation: CAB94216.1. AK002677 mRNA. Translation: BAB22279.1. M11739 mRNA. Translation: AAA37382.1.
IPI	IPI00134741.
PIR	S22563.
RefSeq	NP_112438.1. NM_031161.3.
UniGene	Mm.2619.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P09240.
Proteomic databases
PaxDb	P09240.
PRIDE	P09240.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035120; ENSMUSP00000035120; ENSMUSG00000032532.
GeneID	12424.
KEGG	mmu:12424.
UCSC	uc009sdg.2. mouse.
Organism-specific databases
CTD	885.
MGI	MGI:88297. Cck.
Phylogenomic databases
eggNOG	NOG40485.
GeneTree	ENSGT00390000003571.
HOGENOM	HOG000073533.
HOVERGEN	HBG005806.
KO	K05226.
OMA	QRTDGES.
OrthoDB	EOG4Z8XXW.
Gene expression databases
Bgee	P09240.
CleanEx	MM_CCK.
Genevestigator	P09240.
GermOnline	ENSMUSG00000032532. Mus musculus.
Family and domain databases
InterPro	IPR015499. CCK. IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
PANTHER	PTHR10786. PTHR10786. 1 hit.
Pfam	PF00918. Gastrin. 1 hit. [Graphical view]
SMART	SM00029. GASTRIN. 1 hit. [Graphical view]
PROSITE	PS00259. GASTRIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CCK. mouse.
NextBio	281228.
SOURCE	Search...

Entry information

Entry name

CCKN_MOUSE

Accession

Primary (citable) accession number: P09240
Secondary accession number(s): Q53WS9, Q9DCL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	April 8, 2008
Last modified:	April 3, 2013
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program