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Protein

Cholecystokinin

Gene

Cck

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

GO - Molecular functioni

  1. neuropeptide hormone activity Source: MGI

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  2. axonogenesis Source: MGI
  3. behavioral fear response Source: Ensembl
  4. eating behavior Source: MGI
  5. negative regulation of appetite Source: Ensembl
  6. neuron migration Source: MGI
  7. positive regulation of cell proliferation Source: Ensembl
  8. positive regulation of mitochondrial depolarization Source: Ensembl
  9. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  10. positive regulation of protein oligomerization Source: Ensembl
  11. protein kinase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  12. regulation of sensory perception of pain Source: Ensembl
  13. release of cytochrome c from mitochondria Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_235286. Peptide ligand-binding receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholecystokinin
Short name:
CCK
Cleaved into the following 3 chains:
Cholecystokinin-33
Short name:
CCK33
Cholecystokinin-12
Short name:
CCK12
Cholecystokinin-8
Short name:
CCK8
Gene namesi
Name:Cck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:88297. Cck.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: MGI
  2. axon hillock Source: Ensembl
  3. axon initial segment Source: Ensembl
  4. dendrite Source: Ensembl
  5. extracellular space Source: Ensembl
  6. perikaryon Source: Ensembl
  7. terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 11595CholecystokininPRO_0000010552Add
BLAST
Propeptidei21 – 7050PRO_0000010553Add
BLAST
Peptidei71 – 10333Cholecystokinin-33PRO_0000010554Add
BLAST
Peptidei92 – 10312Cholecystokinin-12PRO_0000010555Add
BLAST
Peptidei96 – 1038Cholecystokinin-8PRO_0000010556
Propeptidei107 – 1159PRO_0000010557

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei97 – 971SulfotyrosineBy similarity
Modified residuei103 – 1031Phenylalanine amide
Modified residuei111 – 1111SulfotyrosineBy similarity
Modified residuei113 – 1131SulfotyrosineBy similarity

Post-translational modificationi

The precursor is cleaved by proteases to produce a number of active cholecystokinins.

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Sulfation

Proteomic databases

PaxDbiP09240.
PRIDEiP09240.

PTM databases

PhosphoSiteiP09240.

Expressioni

Gene expression databases

BgeeiP09240.
CleanExiMM_CCK.
GenevestigatoriP09240.

Interactioni

Subunit structurei

Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain.By similarity

Protein-protein interaction databases

MINTiMINT-1534401.

Family & Domainsi

Sequence similaritiesi

Belongs to the gastrin/cholecystokinin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG40485.
GeneTreeiENSGT00390000003571.
HOGENOMiHOG000073533.
HOVERGENiHBG005806.
InParanoidiP09240.
KOiK05226.
OMAiGRMSIVK.
OrthoDBiEOG7F7WBS.
PhylomeDBiP09240.
TreeFamiTF333419.

Family and domain databases

InterProiIPR015499. CCK_like.
IPR001651. Gastrin.
IPR013152. Gastrin/cholecystokinin_CS.
[Graphical view]
PANTHERiPTHR10786. PTHR10786. 1 hit.
PfamiPF00918. Gastrin. 1 hit.
[Graphical view]
SMARTiSM00029. GASTRIN. 1 hit.
[Graphical view]
PROSITEiPS00259. GASTRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSGVCLCVV MAVLAAGALA QPVVPAEATD PVEQRAQEAP RRQLRAVLRT
60 70 80 90 100
DGEPRARLGA LLARYIQQVR KAPSGRMSVL KNLQSLDPSH RISDRDYMGW
110
MDFGRRSAED YEYPS
Length:115
Mass (Da):12,770
Last modified:April 8, 2008 - v3
Checksum:i7841B11D39BB52DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371Q → E in CAB94216 (PubMed:2011497).Curated
Sequence conflicti50 – 501T → P in CAB94216 (PubMed:2011497).Curated
Sequence conflicti52 – 521G → R in CAB94216 (PubMed:2011497).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59521, X59522 Genomic DNA. Translation: CAB94216.1.
AK002677 mRNA. Translation: BAB22279.1.
M11739 mRNA. Translation: AAA37382.1.
CCDSiCCDS23631.1.
PIRiS22563.
RefSeqiNP_001271437.1. NM_001284508.1.
NP_112438.1. NM_031161.4.
XP_006511991.1. XM_006511928.1.
UniGeneiMm.2619.

Genome annotation databases

EnsembliENSMUST00000035120; ENSMUSP00000035120; ENSMUSG00000032532.
GeneIDi12424.
KEGGimmu:12424.
UCSCiuc009sdg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59521, X59522 Genomic DNA. Translation: CAB94216.1.
AK002677 mRNA. Translation: BAB22279.1.
M11739 mRNA. Translation: AAA37382.1.
CCDSiCCDS23631.1.
PIRiS22563.
RefSeqiNP_001271437.1. NM_001284508.1.
NP_112438.1. NM_031161.4.
XP_006511991.1. XM_006511928.1.
UniGeneiMm.2619.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1534401.

PTM databases

PhosphoSiteiP09240.

Proteomic databases

PaxDbiP09240.
PRIDEiP09240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035120; ENSMUSP00000035120; ENSMUSG00000032532.
GeneIDi12424.
KEGGimmu:12424.
UCSCiuc009sdg.2. mouse.

Organism-specific databases

CTDi885.
MGIiMGI:88297. Cck.

Phylogenomic databases

eggNOGiNOG40485.
GeneTreeiENSGT00390000003571.
HOGENOMiHOG000073533.
HOVERGENiHBG005806.
InParanoidiP09240.
KOiK05226.
OMAiGRMSIVK.
OrthoDBiEOG7F7WBS.
PhylomeDBiP09240.
TreeFamiTF333419.

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_235286. Peptide ligand-binding receptors.

Miscellaneous databases

ChiTaRSiCck. mouse.
NextBioi281228.
PROiP09240.
SOURCEiSearch...

Gene expression databases

BgeeiP09240.
CleanExiMM_CCK.
GenevestigatoriP09240.

Family and domain databases

InterProiIPR015499. CCK_like.
IPR001651. Gastrin.
IPR013152. Gastrin/cholecystokinin_CS.
[Graphical view]
PANTHERiPTHR10786. PTHR10786. 1 hit.
PfamiPF00918. Gastrin. 1 hit.
[Graphical view]
SMARTiSM00029. GASTRIN. 1 hit.
[Graphical view]
PROSITEiPS00259. GASTRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the mouse CCK gene: expression in different brain regions and during cortical development."
    Vitale M., Vashishtha A., Linzer E., Powell D.J., Friedman J.M.
    Nucleic Acids Res. 19:169-177(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/3J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  3. "Differential expression of the mouse cholecystokinin gene during brain and gut development."
    Friedman J.M., Schneider B.S., Powell D.J.
    Proc. Natl. Acad. Sci. U.S.A. 82:5593-5597(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 55-115.

Entry informationi

Entry nameiCCKN_MOUSE
AccessioniPrimary (citable) accession number: P09240
Secondary accession number(s): Q53WS9, Q9DCL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: April 8, 2008
Last modified: January 7, 2015
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.