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P09234

- RU1C_HUMAN

UniProt

P09234 - RU1C_HUMAN

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Protein
U1 small nuclear ribonucleoprotein C
Gene
SNRPC
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates E complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri4 – 3633Matrin-typeUniRule annotation
Add
BLAST

GO - Molecular functioni

  1. mRNA binding Source: UniProtKB-HAMAP
  2. poly(A) RNA binding Source: UniProtKB
  3. protein binding Source: UniProtKB
  4. protein homodimerization activity Source: UniProtKB
  5. single-stranded RNA binding Source: UniProtKB
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. mRNA 5'-splice site recognition Source: UniProtKB-HAMAP
  2. mRNA splicing, via spliceosome Source: UniProtKB
  3. spliceosomal snRNP assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein C
Short name:
U1 snRNP C
Short name:
U1-C
Short name:
U1C
Gene namesi
Name:SNRPC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:11157. SNRPC.

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. Cajal body Source: UniProtKB
  2. U1 snRNP Source: UniProtKB
  3. U2-type prespliceosome Source: UniProtKB-HAMAP
  4. commitment complex Source: UniProtKB-HAMAP
  5. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi6 – 61C → S: Abolishes the binding to U1 snRNP. 1 Publication
Mutagenesisi9 – 91C → S: Abolishes the binding to U1 snRNP. 1 Publication
Mutagenesisi24 – 241H → Q: Abolishes the binding to U1 snRNP. 1 Publication
Mutagenesisi25 – 251C → S: No effect. 1 Publication
Mutagenesisi30 – 301H → Q: Abolishes the binding to U1 snRNP. 1 Publication

Organism-specific databases

PharmGKBiPA35998.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 159159U1 small nuclear ribonucleoprotein CUniRule annotation
PRO_0000097525Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81Phosphotyrosine1 Publication
Modified residuei17 – 171Phosphoserine1 Publication
Modified residuei52 – 521N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP09234.
PaxDbiP09234.
PeptideAtlasiP09234.
PRIDEiP09234.

PTM databases

PhosphoSiteiP09234.

Miscellaneous databases

PMAP-CutDBP09234.

Expressioni

Gene expression databases

ArrayExpressiP09234.
BgeeiP09234.
CleanExiHS_SNRPC.
GenevestigatoriP09234.

Organism-specific databases

HPAiCAB034338.
HPA042419.

Interactioni

Subunit structurei

U1 snRNP is composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. SNRPC/U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RBM10P981752EBI-766589,EBI-721525
WDR83Q9BRX92EBI-766589,EBI-7705033

Protein-protein interaction databases

BioGridi112515. 53 interactions.
DIPiDIP-34235N.
IntActiP09234. 25 interactions.
MINTiMINT-1366350.
STRINGi9606.ENSP00000244520.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni7 – 93
Helixi18 – 247
Helixi29 – 379
Beta strandi39 – 446
Helixi49 – 557
Beta strandi56 – 594

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VRDNMR-A1-61[»]
3CW1X-ray5.490/9/L/l1-77[»]
ProteinModelPortaliP09234.
SMRiP09234. Positions 1-61.

Miscellaneous databases

EvolutionaryTraceiP09234.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 15795Met/Pro-richUniRule annotation
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5136.
HOGENOMiHOG000238970.
HOVERGENiHBG017763.
KOiK11095.
OrthoDBiEOG74TX2B.
TreeFamiTF313578.

Family and domain databases

HAMAPiMF_03153. U1_C.
InterProiIPR017340. U1_snRNP-C.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
IPR013085. Znf_U1-C.
[Graphical view]
PANTHERiPTHR31148. PTHR31148. 1 hit.
PfamiPF06220. zf-U1. 1 hit.
[Graphical view]
PIRSFiPIRSF037969. U1_snRNP-C. 1 hit.
SMARTiSM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09234-1 [UniParc]FASTAAdd to Basket

« Hide

MPKFYCDYCD TYLTHDSPSV RKTHCSGRKH KENVKDYYQK WMEEQAQSLI    50
DKTTAAFQQG KIPPTPFSAP PPAGAMIPPP PSLPGPPRPG MMPAPHMGGP 100
PMMPMMGPPP PGMMPVGPAP GMRPPMGGHM PMMPGPPMMR PPARPMMVPT 150
RPGMTRPDR 159
Length:159
Mass (Da):17,394
Last modified:July 1, 1989 - v1
Checksum:iEC313A57DEC79D95
GO

Sequence cautioni

The sequence CAI20351.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 273CSG → RSR in AAA36618. 1 Publication
Sequence conflicti94 – 985APHMG → TPIW in AAA36618. 1 Publication
Sequence conflicti101 – 1011P → S in AAA36618. 1 Publication
Sequence conflicti129 – 1313HMP → ICQ in AAA36618. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12517 mRNA. Translation: CAA31037.1.
AL139100 Genomic DNA. Translation: CAI20351.1. Sequence problems.
M18465 mRNA. Translation: AAA36618.1.
CCDSiCCDS34436.1.
PIRiS01387.
RefSeqiNP_003084.1. NM_003093.2.
UniGeneiHs.1063.

Genome annotation databases

EnsembliENST00000244520; ENSP00000244520; ENSG00000124562.
GeneIDi6631.
KEGGihsa:6631.
UCSCiuc003ojt.2. human.

Polymorphism databases

DMDMi134093.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12517 mRNA. Translation: CAA31037.1 .
AL139100 Genomic DNA. Translation: CAI20351.1 . Sequence problems.
M18465 mRNA. Translation: AAA36618.1 .
CCDSi CCDS34436.1.
PIRi S01387.
RefSeqi NP_003084.1. NM_003093.2.
UniGenei Hs.1063.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VRD NMR - A 1-61 [» ]
3CW1 X-ray 5.49 0/9/L/l 1-77 [» ]
ProteinModelPortali P09234.
SMRi P09234. Positions 1-61.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112515. 53 interactions.
DIPi DIP-34235N.
IntActi P09234. 25 interactions.
MINTi MINT-1366350.
STRINGi 9606.ENSP00000244520.

PTM databases

PhosphoSitei P09234.

Polymorphism databases

DMDMi 134093.

Proteomic databases

MaxQBi P09234.
PaxDbi P09234.
PeptideAtlasi P09234.
PRIDEi P09234.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000244520 ; ENSP00000244520 ; ENSG00000124562 .
GeneIDi 6631.
KEGGi hsa:6631.
UCSCi uc003ojt.2. human.

Organism-specific databases

CTDi 6631.
GeneCardsi GC06P036301.
H-InvDB HIX0010405.
HIX0171661.
HGNCi HGNC:11157. SNRPC.
HPAi CAB034338.
HPA042419.
MIMi 603522. gene.
neXtProti NX_P09234.
PharmGKBi PA35998.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5136.
HOGENOMi HOG000238970.
HOVERGENi HBG017763.
KOi K11095.
OrthoDBi EOG74TX2B.
TreeFami TF313578.

Miscellaneous databases

EvolutionaryTracei P09234.
GeneWikii Small_nuclear_ribonucleoprotein_polypeptide_C.
GenomeRNAii 6631.
NextBioi 25831.
PMAP-CutDB P09234.
PROi P09234.
SOURCEi Search...

Gene expression databases

ArrayExpressi P09234.
Bgeei P09234.
CleanExi HS_SNRPC.
Genevestigatori P09234.

Family and domain databases

HAMAPi MF_03153. U1_C.
InterProi IPR017340. U1_snRNP-C.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
IPR013085. Znf_U1-C.
[Graphical view ]
PANTHERi PTHR31148. PTHR31148. 1 hit.
Pfami PF06220. zf-U1. 1 hit.
[Graphical view ]
PIRSFi PIRSF037969. U1_snRNP-C. 1 hit.
SMARTi SM00451. ZnF_U1. 1 hit.
[Graphical view ]
PROSITEi PS50171. ZF_MATRIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Human U1 snRNP-specific C protein: complete cDNA and protein sequence and identification of a multigene family in mammals."
    Sillekens P.T.G., Beijer R.P., Habets W.J., van Venrooij W.J.
    Nucleic Acids Res. 16:8307-8321(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Isolation and characterization of a complementary DNA expressing human U1 small nuclear ribonucleoprotein C polypeptide."
    Yamamoto K., Miura H., Moroi Y., Yoshinoya S., Goto M., Nishioka K., Miyamoto T.
    J. Immunol. 140:311-317(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-131.
  4. "U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5' splice site."
    Heinrichs V., Bach M., Winkelmann G., Luehrmann R.
    Science 247:69-72(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Zinc finger-like structure in U1-specific protein C is essential for specific binding to U1 snRNP."
    Nelissen R.L.H., Heinrichs V., Habets W.J., Simons F., Luehrmann R., van Venrooij W.J.
    Nucleic Acids Res. 19:449-454(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-6; CYS-9; HIS-24; CYS-25 AND HIS-30.
  6. "Involvement of U1 small nuclear ribonucleoproteins (snRNP) in 5' splice site-U1 snRNP interaction."
    Rossi F., Forne T., Antoine E., Tazi J., Brunel C., Cathala G.
    J. Biol. Chem. 271:23985-23991(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Protein stoichiometry of a multiprotein complex, the human spliceosomal U1 small nuclear ribonucleoprotein: absolute quantification using isotope-coded tags and mass spectrometry."
    Hochleitner E.O., Kastner B., Froehlich T., Schmidt A., Luehrmann R., Arnold G., Lottspeich F.
    J. Biol. Chem. 280:2536-2542(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE U1 SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "The structure and biochemical properties of the human spliceosomal protein U1C."
    Muto Y., Pomeranz Krummel D., Oubridge C., Hernandez H., Robinson C.V., Neuhaus D., Nagai K.
    J. Mol. Biol. 341:185-198(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-61, SUBUNIT, DOMAIN ZINC-FINGER.
  14. "Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution."
    Pomeranz Krummel D.A., Oubridge C., Leung A.K., Li J., Nagai K.
    Nature 458:475-480(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (5.49 ANGSTROMS) OF 1-77 IN SPLICEOSOMAL U1 SNRNP.

Entry informationi

Entry nameiRU1C_HUMAN
AccessioniPrimary (citable) accession number: P09234
Secondary accession number(s): Q5TAL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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