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P09234

- RU1C_HUMAN

UniProt

P09234 - RU1C_HUMAN

Protein

U1 small nuclear ribonucleoprotein C

Gene

SNRPC

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRPC/U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates E complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri4 – 3633Matrin-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. mRNA binding Source: UniProtKB-HAMAP
    2. poly(A) RNA binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. protein homodimerization activity Source: UniProtKB
    5. single-stranded RNA binding Source: UniProtKB
    6. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. mRNA 5'-splice site recognition Source: UniProtKB-HAMAP
    2. mRNA splicing, via spliceosome Source: UniProtKB
    3. spliceosomal snRNP assembly Source: UniProtKB

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U1 small nuclear ribonucleoprotein CUniRule annotation
    Short name:
    U1 snRNP CUniRule annotation
    Short name:
    U1-CUniRule annotation
    Short name:
    U1CUniRule annotation
    Gene namesi
    Name:SNRPCUniRule annotation
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:11157. SNRPC.

    Subcellular locationi

    GO - Cellular componenti

    1. Cajal body Source: UniProtKB
    2. commitment complex Source: UniProtKB-HAMAP
    3. nucleus Source: HPA
    4. U1 snRNP Source: UniProtKB
    5. U2-type prespliceosome Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi6 – 61C → S: Abolishes the binding to U1 snRNP. 1 Publication
    Mutagenesisi9 – 91C → S: Abolishes the binding to U1 snRNP. 1 Publication
    Mutagenesisi24 – 241H → Q: Abolishes the binding to U1 snRNP. 1 Publication
    Mutagenesisi25 – 251C → S: No effect. 1 Publication
    Mutagenesisi30 – 301H → Q: Abolishes the binding to U1 snRNP. 1 Publication

    Organism-specific databases

    PharmGKBiPA35998.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 159159U1 small nuclear ribonucleoprotein CPRO_0000097525Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei8 – 81Phosphotyrosine1 Publication
    Modified residuei17 – 171Phosphoserine1 Publication
    Modified residuei52 – 521N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP09234.
    PaxDbiP09234.
    PeptideAtlasiP09234.
    PRIDEiP09234.

    PTM databases

    PhosphoSiteiP09234.

    Miscellaneous databases

    PMAP-CutDBP09234.

    Expressioni

    Gene expression databases

    ArrayExpressiP09234.
    BgeeiP09234.
    CleanExiHS_SNRPC.
    GenevestigatoriP09234.

    Organism-specific databases

    HPAiCAB034338.
    HPA042419.

    Interactioni

    Subunit structurei

    U1 snRNP is composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. SNRPC/U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RBM10P981752EBI-766589,EBI-721525
    WDR83Q9BRX92EBI-766589,EBI-7705033

    Protein-protein interaction databases

    BioGridi112515. 53 interactions.
    DIPiDIP-34235N.
    IntActiP09234. 25 interactions.
    MINTiMINT-1366350.
    STRINGi9606.ENSP00000244520.

    Structurei

    Secondary structure

    1
    159
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni7 – 93
    Helixi18 – 247
    Helixi29 – 379
    Beta strandi39 – 446
    Helixi49 – 557
    Beta strandi56 – 594

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2VRDNMR-A1-61[»]
    3CW1X-ray5.490/9/L/l1-77[»]
    ProteinModelPortaliP09234.
    SMRiP09234. Positions 1-61.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09234.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi63 – 15795Met/Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the U1 small nuclear ribonucleoprotein C family.UniRule annotation
    Contains 1 matrin-type zinc finger.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri4 – 3633Matrin-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5136.
    HOGENOMiHOG000238970.
    HOVERGENiHBG017763.
    KOiK11095.
    OrthoDBiEOG74TX2B.
    TreeFamiTF313578.

    Family and domain databases

    HAMAPiMF_03153. U1_C.
    InterProiIPR017340. U1_snRNP-C.
    IPR000690. Znf_C2H2_matrin.
    IPR003604. Znf_U1.
    IPR013085. Znf_U1-C.
    [Graphical view]
    PANTHERiPTHR31148. PTHR31148. 1 hit.
    PfamiPF06220. zf-U1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037969. U1_snRNP-C. 1 hit.
    SMARTiSM00451. ZnF_U1. 1 hit.
    [Graphical view]
    PROSITEiPS50171. ZF_MATRIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09234-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKFYCDYCD TYLTHDSPSV RKTHCSGRKH KENVKDYYQK WMEEQAQSLI    50
    DKTTAAFQQG KIPPTPFSAP PPAGAMIPPP PSLPGPPRPG MMPAPHMGGP 100
    PMMPMMGPPP PGMMPVGPAP GMRPPMGGHM PMMPGPPMMR PPARPMMVPT 150
    RPGMTRPDR 159
    Length:159
    Mass (Da):17,394
    Last modified:July 1, 1989 - v1
    Checksum:iEC313A57DEC79D95
    GO

    Sequence cautioni

    The sequence CAI20351.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 273CSG → RSR in AAA36618. (PubMed:2961811)Curated
    Sequence conflicti94 – 985APHMG → TPIW in AAA36618. (PubMed:2961811)Curated
    Sequence conflicti101 – 1011P → S in AAA36618. (PubMed:2961811)Curated
    Sequence conflicti129 – 1313HMP → ICQ in AAA36618. (PubMed:2961811)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12517 mRNA. Translation: CAA31037.1.
    AL139100 Genomic DNA. Translation: CAI20351.1. Sequence problems.
    M18465 mRNA. Translation: AAA36618.1.
    CCDSiCCDS34436.1.
    PIRiS01387.
    RefSeqiNP_003084.1. NM_003093.2.
    UniGeneiHs.1063.

    Genome annotation databases

    EnsembliENST00000244520; ENSP00000244520; ENSG00000124562.
    GeneIDi6631.
    KEGGihsa:6631.
    UCSCiuc003ojt.2. human.

    Polymorphism databases

    DMDMi134093.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12517 mRNA. Translation: CAA31037.1 .
    AL139100 Genomic DNA. Translation: CAI20351.1 . Sequence problems.
    M18465 mRNA. Translation: AAA36618.1 .
    CCDSi CCDS34436.1.
    PIRi S01387.
    RefSeqi NP_003084.1. NM_003093.2.
    UniGenei Hs.1063.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2VRD NMR - A 1-61 [» ]
    3CW1 X-ray 5.49 0/9/L/l 1-77 [» ]
    ProteinModelPortali P09234.
    SMRi P09234. Positions 1-61.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112515. 53 interactions.
    DIPi DIP-34235N.
    IntActi P09234. 25 interactions.
    MINTi MINT-1366350.
    STRINGi 9606.ENSP00000244520.

    PTM databases

    PhosphoSitei P09234.

    Polymorphism databases

    DMDMi 134093.

    Proteomic databases

    MaxQBi P09234.
    PaxDbi P09234.
    PeptideAtlasi P09234.
    PRIDEi P09234.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000244520 ; ENSP00000244520 ; ENSG00000124562 .
    GeneIDi 6631.
    KEGGi hsa:6631.
    UCSCi uc003ojt.2. human.

    Organism-specific databases

    CTDi 6631.
    GeneCardsi GC06P036301.
    H-InvDB HIX0010405.
    HIX0171661.
    HGNCi HGNC:11157. SNRPC.
    HPAi CAB034338.
    HPA042419.
    MIMi 603522. gene.
    neXtProti NX_P09234.
    PharmGKBi PA35998.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5136.
    HOGENOMi HOG000238970.
    HOVERGENi HBG017763.
    KOi K11095.
    OrthoDBi EOG74TX2B.
    TreeFami TF313578.

    Miscellaneous databases

    EvolutionaryTracei P09234.
    GeneWikii Small_nuclear_ribonucleoprotein_polypeptide_C.
    GenomeRNAii 6631.
    NextBioi 25831.
    PMAP-CutDB P09234.
    PROi P09234.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P09234.
    Bgeei P09234.
    CleanExi HS_SNRPC.
    Genevestigatori P09234.

    Family and domain databases

    HAMAPi MF_03153. U1_C.
    InterProi IPR017340. U1_snRNP-C.
    IPR000690. Znf_C2H2_matrin.
    IPR003604. Znf_U1.
    IPR013085. Znf_U1-C.
    [Graphical view ]
    PANTHERi PTHR31148. PTHR31148. 1 hit.
    Pfami PF06220. zf-U1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037969. U1_snRNP-C. 1 hit.
    SMARTi SM00451. ZnF_U1. 1 hit.
    [Graphical view ]
    PROSITEi PS50171. ZF_MATRIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human U1 snRNP-specific C protein: complete cDNA and protein sequence and identification of a multigene family in mammals."
      Sillekens P.T.G., Beijer R.P., Habets W.J., van Venrooij W.J.
      Nucleic Acids Res. 16:8307-8321(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Isolation and characterization of a complementary DNA expressing human U1 small nuclear ribonucleoprotein C polypeptide."
      Yamamoto K., Miura H., Moroi Y., Yoshinoya S., Goto M., Nishioka K., Miyamoto T.
      J. Immunol. 140:311-317(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-131.
    4. "U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5' splice site."
      Heinrichs V., Bach M., Winkelmann G., Luehrmann R.
      Science 247:69-72(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Zinc finger-like structure in U1-specific protein C is essential for specific binding to U1 snRNP."
      Nelissen R.L.H., Heinrichs V., Habets W.J., Simons F., Luehrmann R., van Venrooij W.J.
      Nucleic Acids Res. 19:449-454(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-6; CYS-9; HIS-24; CYS-25 AND HIS-30.
    6. "Involvement of U1 small nuclear ribonucleoproteins (snRNP) in 5' splice site-U1 snRNP interaction."
      Rossi F., Forne T., Antoine E., Tazi J., Brunel C., Cathala G.
      J. Biol. Chem. 271:23985-23991(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Protein stoichiometry of a multiprotein complex, the human spliceosomal U1 small nuclear ribonucleoprotein: absolute quantification using isotope-coded tags and mass spectrometry."
      Hochleitner E.O., Kastner B., Froehlich T., Schmidt A., Luehrmann R., Arnold G., Lottspeich F.
      J. Biol. Chem. 280:2536-2542(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE U1 SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    8. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-8, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-52, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "The structure and biochemical properties of the human spliceosomal protein U1C."
      Muto Y., Pomeranz Krummel D., Oubridge C., Hernandez H., Robinson C.V., Neuhaus D., Nagai K.
      J. Mol. Biol. 341:185-198(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 1-61, SUBUNIT, DOMAIN ZINC-FINGER.
    14. "Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution."
      Pomeranz Krummel D.A., Oubridge C., Leung A.K., Li J., Nagai K.
      Nature 458:475-480(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (5.49 ANGSTROMS) OF 1-77 IN SPLICEOSOMAL U1 SNRNP.

    Entry informationi

    Entry nameiRU1C_HUMAN
    AccessioniPrimary (citable) accession number: P09234
    Secondary accession number(s): Q5TAL3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3