P09229 (CYT1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine proteinase inhibitor 1

Alternative name(s):
Oryzacystatin I
Short name=OC-I
Oryzacystatin-1
OsCP1
SPOC-I

Gene names

Ordered Locus Names:	Os01g0803200, LOC_Os01g58890
ORF Names:	P0003D09.48, P0034E02.11

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	There are two distinct cystatins in rice seeds (Oryzacystatin-1 and -2) with different specificities against cysteine proteinases. May be involved in the control of germination by inhibition of endogenous cysteine proteinases. May play a role in defense by inhibiting exogenous proteases such as those present in digestive tracks of insects and nematodes. Ref.12
Subcellular location	Secreted Potential.
Developmental stage	Expressed in rice seeds between 2 and 4 weeks after flowering. Ref.12
Biotechnological use	Introduction by genetic manipulation and expression in potato or banana confers resistance to insects and nematodes.
Sequence similarities	Belongs to the cystatin family. Phytocystatin subfamily. Contains 1 cystatin domain.
Sequence caution	The sequence AAA33903.1 differs from that shown. Reason: Erroneous initiation. The sequence AAA33912.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB24010.1 differs from that shown. Reason: Erroneous initiation. The sequence AAB66355.1 differs from that shown. Reason: Frameshift at position 18. The sequence AAL30830.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB86438.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB92242.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Protease inhibitor Thiol protease inhibitor
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P09229-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P09229-2)

The sequence of this isoform differs from the canonical sequence as follows:
     77-96: NSLLEFEKLVSVKQQVVAGT → VSAPLYPPPYFSSSQGRSSN
     97-140: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Potential

Chain

27 – 140

114

Cysteine proteinase inhibitor 1

PRO_0000207156

Regions

Domain

48 – 135

88

Cystatin

Motif

91 – 95

5

Secondary area of contact

Sites

Site

48

1

Reactive site

Natural variations

Alternative sequence

77 – 96

20

NSLLE…VVAGT → VSAPLYPPPYFSSSQGRSSN in isoform 2.

VSP_023023

Alternative sequence

97 – 140

44

Missing in isoform 2.

VSP_023024

Experimental info

Mutagenesis

124

1

Missing: Increase in inhibition activity by 13-fold. Ref.13

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

140

Helix Strand Turn

Details...

Beta strand

47 – 53

7

Helix

59 – 75

17

Beta strand

80 – 106

27

Beta strand

111 – 120

10

Turn

121 – 124

4

Beta strand

128 – 132

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 6, 2007. Version 2. Checksum: 541263986E70C8FB	FASTA	140	15,355
	10 20 30 40 50 60 MRKYRVAGLV AALLVLHSLA TPSAQAEAHR AGGEGEEKMS SDGGPVLGGV EPVGNENDLH 70 80 90 100 110 120 LVDLARFAVT EHNKKANSLL EFEKLVSVKQ QVVAGTLYYF TIEVKEGDAK KLYEAKVWEK 130 140 PWMDFKELQE FKPVDASANA
	Isoform 2 [UniParc]. Checksum: 72CB22009D726078	FASTA	96	10,068
	10 20 30 40 50 60 MRKYRVAGLV AALLVLHSLA TPSAQAEAHR AGGEGEEKMS SDGGPVLGGV EPVGNENDLH 70 80 90 LVDLARFAVT EHNKKAVSAP LYPPPYFSSS QGRSSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of the genomic DNA fragment encoding oryzacystatin." Kondo H., Emori Y., Abe K., Suzuki K., Arai S. Gene 81:259-265(1989) [PubMed: 2806916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Nipponbare.
[6]	"Oryzacystatin-I has a signal peptide." Womack J.S., Randall J., Kemp J.D. Plant Gene Register PGR99-175 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
[7]	"Identification of a signal peptide for oryzacystatin-I." Womack J.S., Randall J., Kemp J.D. Planta 210:844-847(2000) [PubMed: 10805458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
[8]	"High level expression of oryzacystatin in Escherichia coli." Zhou Z., Zhu Z., Chen R., Liu C., Li X. Chin. J. Biotechnol. 12:17-24(1996) [PubMed: 8877110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-140 (ISOFORM 1). Tissue: Seed.
[9]	"OsCPI, a rice blast inducible cysteine proteinase inhibitor." Peng Y.L., Qin Q.M., Su S.C., Li H. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-140 (ISOFORM 1). Strain: cv. Aichiasahi.
[10]	"Rice cystatin: bacterial expression, purification, cysteine proteinase inhibitory activity, and insect growth suppressing activity of a truncated form of the protein." Chen M.S., Johnson B., Wen L., Muthukrishnan S., Kramer K.J., Morgan T.D., Reeck G.R. Protein Expr. Purif. 3:41-49(1992) [PubMed: 1422207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-140 (ISOFORM 1).
[11]	"Molecular cloning of a cysteine proteinase inhibitor of rice (oryzacystatin). Homology with animal cystatins and transient expression in the ripening process of rice seeds." Abe K., Emori Y., Kondo H., Suzuki K., Arai S. J. Biol. Chem. 262:16793-16797(1987) [PubMed: 3500172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-140 (ISOFORM 1), PARTIAL PROTEIN SEQUENCE. Strain: cv. Nipponbare. Tissue: Seed.
[12]	"Two distinct cystatin species in rice seeds with different specificities against cysteine proteinases. Molecular cloning, expression, and biochemical studies on oryzacystatin-II." Kondo H., Abe K., Nishimura I., Watanabe H., Emori Y., Arai S. J. Biol. Chem. 265:15832-15837(1990) [PubMed: 1697595] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[13]	"Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida." Urwin P.E., Atkinson H.J., Waller D.A., McPherson M.J. Plant J. 8:121-131(1995) [PubMed: 7655503] [Abstract] Cited for: MUTAGENESIS OF ASP-124.
[14]	"Comparative phylogenetic analysis of cystatin gene families from arabidopsis, rice and barley." Martinez M., Abraham Z., Carbonero P., Diaz I. Mol. Genet. Genomics 273:423-432(2005) [PubMed: 15887031] [Abstract] Cited for: GENE FAMILY.
[15]	"Three-dimensional solution structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica." Nagata K., Kudo N., Abe K., Arai S., Tanokura M. Biochemistry 39:14753-14760(2000) [PubMed: 11101290] [Abstract] Cited for: STRUCTURE BY NMR OF 39-140.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M29259 Genomic DNA. Translation: AAA33912.1. Different initiation.
AP003221 Genomic DNA. Translation: BAB86438.1. Different initiation.
AP003232 Genomic DNA. Translation: BAB92242.1. Different initiation.
AP008207 Genomic DNA. Translation: BAF06464.1.
AK061770 mRNA. No translation available.
AF164378 Genomic DNA. Translation: AAF18388.1.
U54702 mRNA. Translation: AAB66355.1. Frameshift.
AF435976 mRNA. Translation: AAL30830.1. Different initiation.
S49967 mRNA. Translation: AAB24010.1. Different initiation.
J03469 mRNA. Translation: AAA33903.1. Different initiation.

PIR

A28464. B28464.

RefSeq

NP_001044550.1. NM_001051085.1.

UniGene

Os.1316.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EQK	NMR	-	A	39-140	[»]

ProteinModelPortal

P09229.

SMR

P09229. Positions 39-140.

ModBase

Search...

Protein-protein interaction databases

STRING

P09229.

Protein family/group databases

MEROPS

I25.052.

Proteomic databases

PRIDE

P09229.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

4327535.

KEGG

osa:4327535.

Organism-specific databases

Gramene

P09229.

Phylogenomic databases

HOGENOM

HBG602279.

OMA

LAICRSE.

PhylomeDB

P09229.

Family and domain databases

InterPro

IPR000010. Prot_inh_cystat.
IPR020381. Prot_inh_cystat_cons-reg.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]

Pfam

PF00031. Cystatin. 1 hit.
[Graphical view]

ProDom

PD001231. Prot_inh_cystat_cons-reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00043. CY. 1 hit.
[Graphical view]

PROSITE

PS00287. CYSTATIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CYT1_ORYSJ

Accession

Primary (citable) accession number: P09229
Secondary accession number(s): Q0JIG4 , Q547V7, Q7F5W6, Q9SBW0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program