Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lymphotoxin-alpha

Gene

Lta

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.

GO - Molecular functioni

  • tumor necrosis factor receptor binding Source: MGI

GO - Biological processi

  • cell proliferation Source: MGI
  • cellular process Source: MGI
  • defense response to Gram-positive bacterium Source: MGI
  • humoral immune response Source: MGI
  • inflammatory response Source: MGI
  • lymph node development Source: MGI
  • negative regulation of fibroblast proliferation Source: Ensembl
  • negative regulation of growth of symbiont in host Source: MGI
  • positive regulation of apoptotic process Source: Ensembl
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of chronic inflammatory response to antigenic stimulus Source: MGI
  • positive regulation of glial cell proliferation Source: Ensembl
  • positive regulation of humoral immune response mediated by circulating immunoglobulin Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • response to drug Source: Ensembl
  • response to hypoxia Source: Ensembl
  • response to lipopolysaccharide Source: Ensembl
  • response to nutrient Source: Ensembl
  • transformed cell apoptotic process Source: MGI
  • tumor necrosis factor-mediated signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Enzyme and pathway databases

ReactomeiR-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5669034. TNFs bind their physiological receptors.
R-MMU-5676594. TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphotoxin-alpha
Short name:
LT-alpha
Alternative name(s):
TNF-beta
Tumor necrosis factor ligand superfamily member 1
Gene namesi
Name:Lta
Synonyms:Tnfb, Tnfsf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:104797. Lta.

Subcellular locationi

  • Secreted By similarity
  • Membrane By similarity

  • Note: The homotrimer is secreted. The heterotrimer is membrane-associated.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Add
BLAST
Chaini34 – 202169Lymphotoxin-alphaPRO_0000034467Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP09225.
PaxDbiP09225.
PRIDEiP09225.

Expressioni

Gene expression databases

BgeeiP09225.
CleanExiMM_LTA.
ExpressionAtlasiP09225. baseline and differential.
GenevisibleiP09225. MM.

Interactioni

Subunit structurei

Homotrimer, and heterotrimer of either two LTB and one LTA subunits or (less prevalent) two LTA and one LTB subunits.By similarity

GO - Molecular functioni

  • tumor necrosis factor receptor binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025266.

Structurei

3D structure databases

ProteinModelPortaliP09225.
SMRiP09225. Positions 59-202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IWT9. Eukaryota.
ENOG410YQC4. LUCA.
HOGENOMiHOG000048729.
HOVERGENiHBG012516.
InParanoidiP09225.
KOiK05468.
OMAiFPKATPT.
OrthoDBiEOG7V4B0Q.
PhylomeDBiP09225.
TreeFamiTF332169.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR006053. TNF.
IPR002960. TNF_beta.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PRINTSiPR01234. TNECROSISFCT.
PR01236. TNFBETA.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09225-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLGRLHLL RVLGTPPVFL LGLLLALPLG AQGLSGVRFS AARTAHPLPQ
60 70 80 90 100
KHLTHGILKP AAHLVGYPSK QNSLLWRAST DRAFLRHGFS LSNNSLLIPT
110 120 130 140 150
SGLYFVYSQV VFSGESCSPR AIPTPIYLAH EVQLFSSQYP FHVPLLSAQK
160 170 180 190 200
SVYPGLQGPW VRSMYQGAVF LLSKGDQLST HTDGISHLHF SPSSVFFGAF

AL
Length:202
Mass (Da):21,999
Last modified:July 1, 1989 - v1
Checksum:iF496F83C685950D3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261A → P in AAA39450 (PubMed:2885372).Curated
Sequence conflicti161 – 1622VR → CG in CAA29567 (PubMed:3490653).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06950 Unassigned DNA. Translation: AAA18593.1.
Y00467 Genomic DNA. Translation: CAA68529.1.
X06217 Genomic DNA. Translation: CAA29566.1.
X06218 Genomic DNA. Translation: CAA29567.1.
Y00137 Genomic DNA. Translation: CAA68330.1.
M16819 mRNA. Translation: AAA40460.1.
M17015 Genomic DNA. Translation: AAA39450.1.
AF109719 Genomic DNA. Translation: AAC82485.1.
X14800 mRNA. Translation: CAA32906.1.
M60586 Genomic DNA. Translation: AAA40461.1.
CCDSiCCDS28692.1.
PIRiB27303.
RefSeqiNP_034865.1. NM_010735.2.
XP_006523794.1. XM_006523731.2.
XP_006525394.1. XM_006525331.2.
XP_006536613.1. XM_006536550.2.
XP_006537347.1. XM_006537284.2.
UniGeneiMm.87787.

Genome annotation databases

EnsembliENSMUST00000025266; ENSMUSP00000025266; ENSMUSG00000024402.
GeneIDi16992.
KEGGimmu:16992.
UCSCiuc008cgu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06950 Unassigned DNA. Translation: AAA18593.1.
Y00467 Genomic DNA. Translation: CAA68529.1.
X06217 Genomic DNA. Translation: CAA29566.1.
X06218 Genomic DNA. Translation: CAA29567.1.
Y00137 Genomic DNA. Translation: CAA68330.1.
M16819 mRNA. Translation: AAA40460.1.
M17015 Genomic DNA. Translation: AAA39450.1.
AF109719 Genomic DNA. Translation: AAC82485.1.
X14800 mRNA. Translation: CAA32906.1.
M60586 Genomic DNA. Translation: AAA40461.1.
CCDSiCCDS28692.1.
PIRiB27303.
RefSeqiNP_034865.1. NM_010735.2.
XP_006523794.1. XM_006523731.2.
XP_006525394.1. XM_006525331.2.
XP_006536613.1. XM_006536550.2.
XP_006537347.1. XM_006537284.2.
UniGeneiMm.87787.

3D structure databases

ProteinModelPortaliP09225.
SMRiP09225. Positions 59-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025266.

Proteomic databases

EPDiP09225.
PaxDbiP09225.
PRIDEiP09225.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025266; ENSMUSP00000025266; ENSMUSG00000024402.
GeneIDi16992.
KEGGimmu:16992.
UCSCiuc008cgu.2. mouse.

Organism-specific databases

CTDi4049.
MGIiMGI:104797. Lta.

Phylogenomic databases

eggNOGiENOG410IWT9. Eukaryota.
ENOG410YQC4. LUCA.
HOGENOMiHOG000048729.
HOVERGENiHBG012516.
InParanoidiP09225.
KOiK05468.
OMAiFPKATPT.
OrthoDBiEOG7V4B0Q.
PhylomeDBiP09225.
TreeFamiTF332169.

Enzyme and pathway databases

ReactomeiR-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5669034. TNFs bind their physiological receptors.
R-MMU-5676594. TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.

Miscellaneous databases

PROiP09225.
SOURCEiSearch...

Gene expression databases

BgeeiP09225.
CleanExiMM_LTA.
ExpressionAtlasiP09225. baseline and differential.
GenevisibleiP09225. MM.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR006053. TNF.
IPR002960. TNF_beta.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PRINTSiPR01234. TNECROSISFCT.
PR01236. TNFBETA.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the murine TNF locus, including the TNF-alpha (tumor necrosis factor) and TNF-beta (lymphotoxin) genes."
    Semon D., Kawashima E., Jongeneel C.V., Shakhov A.N., Nedospasov S.A.
    Nucleic Acids Res. 15:9083-9084(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genes for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) are tandemly arranged on chromosome 17 of the mouse."
    Nedospasov S.A., Hirt B., Shakhov A.N., Dobrynin V.N., Kawashima E., Accolla R.S., Jongeneel C.V.
    Nucleic Acids Res. 14:7713-7725(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The murine tumor necrosis factor-beta (lymphotoxin) gene sequence."
    Gray P.W., Chen E., Tang W.-L., Ruddle N.H.
    Nucleic Acids Res. 15:3937-3937(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning and expression of murine lymphotoxin cDNA."
    Li C.B., Gray P.W., Lin P.F., McGrath K.M., Ruddle F.H., Ruddle N.H.
    J. Immunol. 138:4496-4501(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Mouse lymphotoxin and tumor necrosis factor: structural analysis of the cloned genes, physical linkage, and chromosomal position."
    Gardner S.M., Mock B.A., Hilgers J., Huppi K.E., Roeder W.D.
    J. Immunol. 139:476-483(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129.
  7. "Induction of tumor necrosis factor-alpha and -beta and interferon-gamma mRNA by interleukin 2 in murine lymphocytic cell lines."
    Weil D., Dautry F.
    Oncogene Res. 3:409-414(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 6-202.
  8. "RNA processing is a limiting step for murine tumor necrosis factor beta expression in response to interleukin-2."
    Weil D., Brosset S., Dautry F.
    Mol. Cell. Biol. 10:5865-5875(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 56-76.

Entry informationi

Entry nameiTNFB_MOUSE
AccessioniPrimary (citable) accession number: P09225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 8, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.