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Protein

Fructose-1,6-bisphosphatase

Gene

FBP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. AMP binding affects the turnover of bound substrate and not the affinity for substrate (By similarity).By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Magnesium 1By similarity1
Metal bindingi108Magnesium 1By similarity1
Metal bindingi108Magnesium 2By similarity1
Metal bindingi128Magnesium 2By similarity1
Metal bindingi128Magnesium 3By similarity1
Metal bindingi130Magnesium 2; via carbonyl oxygenBy similarity1
Metal bindingi131Magnesium 3By similarity1
Binding sitei222SubstrateBy similarity1
Binding sitei256SubstrateBy similarity1
Binding sitei276SubstrateBy similarity1
Binding sitei286SubstrateBy similarity1
Metal bindingi292Magnesium 3By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 42AMPBy similarity5
Nucleotide bindingi122 – 123AMPBy similarity2

GO - Molecular functioni

  • fructose 1,6-bisphosphate 1-phosphatase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 1,6-bisphosphate metabolic process Source: GO_Central
  • fructose 6-phosphate metabolic process Source: GO_Central
  • fructose catabolic process Source: InterPro
  • fructose metabolic process Source: GO_Central
  • gluconeogenesis Source: SGD
  • reactive oxygen species metabolic process Source: SGD
  • sucrose biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YLR377C-MONOMER.
BRENDAi3.1.3.11. 984.
ReactomeiR-SCE-70263. Gluconeogenesis.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphatase (EC:3.1.3.11)
Short name:
FBPase
Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase
Gene namesi
Name:FBP1
Ordered Locus Names:YLR377C
ORF Names:L8039.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR377C.
SGDiS000004369. FBP1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • periplasmic space Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002005112 – 348Fructose-1,6-bisphosphataseAdd BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP09201.

PTM databases

iPTMnetiP09201.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi31636. 64 interactors.
DIPiDIP-3986N.
IntActiP09201. 6 interactors.
MINTiMINT-562945.

Structurei

3D structure databases

ProteinModelPortaliP09201.
SMRiP09201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 134Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the FBPase class 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000015513.
HOGENOMiHOG000191265.
InParanoidiP09201.
KOiK03841.
OMAiGTIFAIH.
OrthoDBiEOG092C350K.

Family and domain databases

CDDicd00354. FBPase. 1 hit.
HAMAPiMF_01855. FBPase_class1. 1 hit.
InterProiIPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
[Graphical view]
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiPF00316. FBPase. 1 hit.
[Graphical view]
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiPS00124. FBPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTLVNGPRR DSTEGFDTDI ITLPRFIIEH QKQFKNATGD FTLVLNALQF
60 70 80 90 100
AFKFVSHTIR RAELVNLVGL AGASNFTGDQ QKKLDVLGDE IFINAMRASG
110 120 130 140 150
IIKVLVSEEQ EDLIVFPTNT GSYAVCCDPI DGSSNLDAGV SVGTIASIFR
160 170 180 190 200
LLPDSSGTIN DVLRCGKEMV AACYAMYGSS THLVLTLGDG VDGFTLDTNL
210 220 230 240 250
GEFILTHPNL RIPPQKAIYS INEGNTLYWN ETIRTFIEKV KQPQADNNNK
260 270 280 290 300
PFSARYVGSM VADVHRTFLY GGLFAYPCDK KSPNGKLRLL YEAFPMAFLM
310 320 330 340
EQAGGKAVND RGERILDLVP SHIHDKSSIW LGSSGEIDKF LDHIGKSQ
Length:348
Mass (Da):38,263
Last modified:January 23, 2007 - v2
Checksum:iBCFC0984A086094B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti278C → D AA sequence (PubMed:3008716).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00754 Genomic DNA. Translation: CAA68723.1.
J03207 Genomic DNA. Translation: AAA34603.1.
U19103 Genomic DNA. Translation: AAB67579.1.
AY692816 Genomic DNA. Translation: AAT92835.1.
BK006945 Genomic DNA. Translation: DAA09679.1.
PIRiS01127. PABY.
RefSeqiNP_013481.3. NM_001182266.3.

Genome annotation databases

EnsemblFungiiYLR377C; YLR377C; YLR377C.
GeneIDi851092.
KEGGisce:YLR377C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00754 Genomic DNA. Translation: CAA68723.1.
J03207 Genomic DNA. Translation: AAA34603.1.
U19103 Genomic DNA. Translation: AAB67579.1.
AY692816 Genomic DNA. Translation: AAT92835.1.
BK006945 Genomic DNA. Translation: DAA09679.1.
PIRiS01127. PABY.
RefSeqiNP_013481.3. NM_001182266.3.

3D structure databases

ProteinModelPortaliP09201.
SMRiP09201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31636. 64 interactors.
DIPiDIP-3986N.
IntActiP09201. 6 interactors.
MINTiMINT-562945.

PTM databases

iPTMnetiP09201.

Proteomic databases

PRIDEiP09201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR377C; YLR377C; YLR377C.
GeneIDi851092.
KEGGisce:YLR377C.

Organism-specific databases

EuPathDBiFungiDB:YLR377C.
SGDiS000004369. FBP1.

Phylogenomic databases

GeneTreeiENSGT00390000015513.
HOGENOMiHOG000191265.
InParanoidiP09201.
KOiK03841.
OMAiGTIFAIH.
OrthoDBiEOG092C350K.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciYEAST:YLR377C-MONOMER.
BRENDAi3.1.3.11. 984.
ReactomeiR-SCE-70263. Gluconeogenesis.

Miscellaneous databases

PROiP09201.

Family and domain databases

CDDicd00354. FBPase. 1 hit.
HAMAPiMF_01855. FBPase_class1. 1 hit.
InterProiIPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
[Graphical view]
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiPF00316. FBPase. 1 hit.
[Graphical view]
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiPS00124. FBPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF16P_YEAST
AccessioniPrimary (citable) accession number: P09201
Secondary accession number(s): D6VZ13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.