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Protein

Cytochrome b559 subunit beta

Gene

psbF

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This b-type cytochrome is tightly associated with the reaction center of photosystem II (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation.UniRule annotation

Cofactori

heme bUniRule annotationNote: With its partner (PsbE) binds heme. PSII binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Iron (heme axial ligand); shared with alpha subunitUniRule annotation1 Publication

GO - Molecular functioni

  • electron carrier activity Source: UniProtKB-HAMAP
  • heme binding Source: InterPro
  • identical protein binding Source: IntAct
  • iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b559 subunit betaUniRule annotation
Alternative name(s):
PSII reaction center subunit VIUniRule annotation
Gene namesi
Name:psbFUniRule annotation
Ordered Locus Names:smr0006
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

Subcellular locationi

  • Cellular thylakoid membrane UniRule annotation1 Publication; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei19 – 3517HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231H → L: Complete loss of PSII activity, decreased accumulation of PSII subunits, altered mobility of the PsbE subunit. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 4443Cytochrome b559 subunit betaPRO_0000200478Add
BLAST

Interactioni

Subunit structurei

Heterodimer of an alpha subunit and a beta subunit. Cyanobacterial PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms dimeric complexes.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-701712,EBI-701712

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

IntActiP09191. 2 interactions.
MINTiMINT-8416997.

Structurei

3D structure databases

ProteinModelPortaliP09191.
SMRiP09191. Positions 8-44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PsbE/PsbF family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000091420.
InParanoidiP09191.
KOiK02708.

Family and domain databases

HAMAPiMF_00643. PSII_PsbF. 1 hit.
InterProiIPR006241. PSII_cyt_b559_bsu.
IPR006216. PSII_cyt_b559_CS.
IPR013081. PSII_cyt_b559_N.
[Graphical view]
PfamiPF00283. Cytochrom_B559. 1 hit.
[Graphical view]
PIRSFiPIRSF000037. PsbF. 1 hit.
TIGRFAMsiTIGR01333. cyt_b559_beta. 1 hit.
PROSITEiPS00537. CYTOCHROME_B559. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
MATQNPNQPV TYPIFTVRWL AVHTLAVPSV FFVGAIAAMQ FIQR
Length:44
Mass (Da):4,933
Last modified:January 23, 2007 - v3
Checksum:i9A4E1F762B213436
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33897 Genomic DNA. Translation: AAA27300.1.
BA000022 Genomic DNA. Translation: BAA17093.1.
PIRiS00339. F2YB4.

Genome annotation databases

EnsemblBacteriaiBAA17093; BAA17093; BAA17093.
KEGGisyn:smr0006.
PATRICi23837986. VBISynSp132158_0562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33897 Genomic DNA. Translation: AAA27300.1.
BA000022 Genomic DNA. Translation: BAA17093.1.
PIRiS00339. F2YB4.

3D structure databases

ProteinModelPortaliP09191.
SMRiP09191. Positions 8-44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09191. 2 interactions.
MINTiMINT-8416997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17093; BAA17093; BAA17093.
KEGGisyn:smr0006.
PATRICi23837986. VBISynSp132158_0562.

Phylogenomic databases

HOGENOMiHOG000091420.
InParanoidiP09191.
KOiK02708.

Family and domain databases

HAMAPiMF_00643. PSII_PsbF. 1 hit.
InterProiIPR006241. PSII_cyt_b559_bsu.
IPR006216. PSII_cyt_b559_CS.
IPR013081. PSII_cyt_b559_N.
[Graphical view]
PfamiPF00283. Cytochrom_B559. 1 hit.
[Graphical view]
PIRSFiPIRSF000037. PsbF. 1 hit.
TIGRFAMsiTIGR01333. cyt_b559_beta. 1 hit.
PROSITEiPS00537. CYTOCHROME_B559. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSBF_SYNY3
AccessioniPrimary (citable) accession number: P09191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.