P09191 (PSBF_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b559 subunit beta

Alternative name(s):
PSII reaction center subunit VI

Gene names

Name:	psbF
Ordered Locus Names:	smr0006

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	44 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This b-type cytochrome is tightly associated with the reaction center of photosystem II and possibly is part of the water-oxidation complex By similarity. HAMAP-Rule MF_00643
Subunit structure	Heterodimer of an alpha subunit and a beta subunit By similarity.
Subcellular location	Cellular thylakoid membrane; Single-pass membrane protein By similarity HAMAP-Rule MF_00643.
Sequence similarities	Belongs to the PsbE/PsbF family.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Membrane Photosystem II Thylakoid
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	photosynthetic electron transport chain Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW photosystem II reaction center Inferred from electronic annotation. Source: InterPro plasma membrane-derived thylakoid photosystem II Inferred from direct assay PubMed 12069591. Source: UniProtKB thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-701712,EBI-701712

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed HAMAP-Rule MF_00643
	Chain	2 – 44	43	Cytochrome b559 subunit beta HAMAP-Rule MF_00643		PRO_0000200478
Regions
	Topological domain	2 – 18	17	Stromal Potential
	Transmembrane	19 – 39	21	Helical; Potential
	Topological domain	40 – 44	5	Lumenal Potential
Sites
	Metal binding	23	1	Iron (heme axial ligand); shared with alpha subunit
Experimental info
	Mutagenesis	23	1	H → L: Complete loss of activity. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P09191 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9A4E1F762B213436	FASTA	44	4,933
	10 20 30 40 MATQNPNQPV TYPIFTVRWL AVHTLAVPSV FFVGAIAAMQ FIQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II." Pakrasi H.B., Williams J.G.K., Arntzen C.J. EMBO J. 7:325-332(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.
[3]	"Site directed mutagenesis of the heme axial ligands of cytochrome b559 affects the stability of the photosystem II complex." Pakrasi H.B., de Ciechi P., Whitmarsh J. EMBO J. 10:1619-1627(1991) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF HIS-23.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33897 Genomic DNA. Translation: AAA27300.1. BA000022 Genomic DNA. Translation: BAA17093.1.
PIR	F2YB4. S00339.
RefSeq	NP_440413.1. NC_000911.1. YP_005650471.1. NC_017277.1. YP_007450297.1. NC_020286.1.
3D structure databases
ProteinModelPortal	P09191.
SMR	P09191. Positions 8-44.
ModBase	Search...
Protein-protein interaction databases
IntAct	P09191. 2 interactions.
STRING	1148.smr0006.
Proteomic databases
PaxDb	P09191.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA17093; BAA17093; BAA17093.
GeneID	12256161. 14615945. 953713.
KEGG	syn:smr0006. syy:SYNGTS_0518.
PATRIC	23837986. VBISynSp132158_0562.
Phylogenomic databases
eggNOG	NOG09649.
HOGENOM	HOG000091420.
KO	K02708.
ProtClustDB	PRK02561.
Family and domain databases
HAMAP	MF_00643. PSII_PsbF.
InterPro	IPR006241. PSII_cyt_b559_bsu. IPR006216. PSII_cyt_b559_CS. IPR013081. PSII_cyt_b559_N. [Graphical view]
Pfam	PF00283. Cytochrom_B559. 1 hit. [Graphical view]
PIRSF	PIRSF000037. PsbF. 1 hit.
TIGRFAMs	TIGR01333. cyt_b559_beta. 1 hit.
PROSITE	PS00537. CYTOCHROME_B559. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSBF_SYNY3

Accession

Primary (citable) accession number: P09191

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program