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Protein

Cytochrome b559 subunit beta

Gene

psbF

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This b-type cytochrome is tightly associated with the reaction center of photosystem II (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation.UniRule annotation

Cofactori

heme bUniRule annotationNote: With its partner (PsbE) binds heme. PSII binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Iron (heme axial ligand); shared with alpha subunit1 PublicationUniRule annotation

GO - Molecular functioni

  1. electron carrier activity Source: UniProtKB-HAMAP
  2. heme binding Source: InterPro
  3. identical protein binding Source: IntAct
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. photosynthetic electron transport chain Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b559 subunit betaUniRule annotation
Alternative name(s):
PSII reaction center subunit VIUniRule annotation
Gene namesi
Name:psbFUniRule annotation
Ordered Locus Names:smr0006
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis
ProteomesiUP000001425: Chromosome

Subcellular locationi

Cellular thylakoid membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei19 – 3517HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. photosystem II reaction center Source: InterPro
  3. plasma membrane-derived thylakoid photosystem II Source: UniProtKB
  4. thylakoid membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231H → L: Complete loss of PSII activity, decreased accumulation of PSII subunits, altered mobility of the PsbE subunit. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4444Cytochrome b559 subunit betaPRO_0000200478Add
BLAST

Proteomic databases

PaxDbiP09191.

Interactioni

Subunit structurei

Heterodimer of an alpha subunit and a beta subunit. Cyanobacterial PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms dimeric complexes.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-701712,EBI-701712

Protein-protein interaction databases

IntActiP09191. 2 interactions.
MINTiMINT-8416997.
STRINGi1148.smr0006.

Structurei

3D structure databases

ProteinModelPortaliP09191.
SMRiP09191. Positions 8-44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PsbE/PsbF family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG09649.
HOGENOMiHOG000091420.
InParanoidiP09191.
KOiK02708.
OrthoDBiEOG6B0B10.

Family and domain databases

HAMAPiMF_00643. PSII_PsbF.
InterProiIPR006241. PSII_cyt_b559_bsu.
IPR006216. PSII_cyt_b559_CS.
IPR013081. PSII_cyt_b559_N.
[Graphical view]
PfamiPF00283. Cytochrom_B559. 1 hit.
[Graphical view]
PIRSFiPIRSF000037. PsbF. 1 hit.
TIGRFAMsiTIGR01333. cyt_b559_beta. 1 hit.
PROSITEiPS00537. CYTOCHROME_B559. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09191-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40 
MATQNPNQPV TYPIFTVRWL AVHTLAVPSV FFVGAIAAMQ FIQR
Length:44
Mass (Da):4,933
Last modified:January 23, 2007 - v3
Checksum:i9A4E1F762B213436
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33897 Genomic DNA. Translation: AAA27300.1.
BA000022 Genomic DNA. Translation: BAA17093.1.
PIRiS00339. F2YB4.
RefSeqiNP_440413.1. NC_000911.1.
YP_005650471.1. NC_017277.1.
YP_007450297.1. NC_020286.1.

Genome annotation databases

EnsemblBacteriaiBAA17093; BAA17093; BAA17093.
GeneIDi953713.
KEGGisyn:smr0006.
PATRICi23837986. VBISynSp132158_0562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33897 Genomic DNA. Translation: AAA27300.1.
BA000022 Genomic DNA. Translation: BAA17093.1.
PIRiS00339. F2YB4.
RefSeqiNP_440413.1. NC_000911.1.
YP_005650471.1. NC_017277.1.
YP_007450297.1. NC_020286.1.

3D structure databases

ProteinModelPortaliP09191.
SMRiP09191. Positions 8-44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09191. 2 interactions.
MINTiMINT-8416997.
STRINGi1148.smr0006.

Proteomic databases

PaxDbiP09191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA17093; BAA17093; BAA17093.
GeneIDi953713.
KEGGisyn:smr0006.
PATRICi23837986. VBISynSp132158_0562.

Phylogenomic databases

eggNOGiNOG09649.
HOGENOMiHOG000091420.
InParanoidiP09191.
KOiK02708.
OrthoDBiEOG6B0B10.

Family and domain databases

HAMAPiMF_00643. PSII_PsbF.
InterProiIPR006241. PSII_cyt_b559_bsu.
IPR006216. PSII_cyt_b559_CS.
IPR013081. PSII_cyt_b559_N.
[Graphical view]
PfamiPF00283. Cytochrom_B559. 1 hit.
[Graphical view]
PIRSFiPIRSF000037. PsbF. 1 hit.
TIGRFAMsiTIGR01333. cyt_b559_beta. 1 hit.
PROSITEiPS00537. CYTOCHROME_B559. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II."
    Pakrasi H.B., Williams J.G.K., Arntzen C.J.
    EMBO J. 7:325-332(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
    Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.
    , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 6803 / Kazusa.
  3. "Site directed mutagenesis of the heme axial ligands of cytochrome b559 affects the stability of the photosystem II complex."
    Pakrasi H.B., de Ciechi P., Whitmarsh J.
    EMBO J. 10:1619-1627(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-23.

Entry informationi

Entry nameiPSBF_SYNY3
AccessioniPrimary (citable) accession number: P09191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Synechocystis PCC 6803
    Synechocystis (strain PCC 6803): entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.